Adult ; Chemical and Drug Induced Liver Injury ; Dimethylformamide ; poisoning ; Humans ; Male

Objective: To study the biocompatibility of CFR/PEEK composite in bone tissue after implanted in lumbar intervertebral space and to evaluate its role in the interbody fusion compared to the allograft bone. Methods: Thirteen beagles were chosen among which 7 were implanted with the disk-like CFR/PEEK composite in the lumbar intervertebral space and the other 6 were implanted with allograft bone. X-ray, QCT and histological examination were employed at 6, 12 and 24 months postoperatively. Results: The X-ray results of fusion segment were in conformity with the QCT's as well as that of histological results. All animals obtained a complete fusion at 24 months. Histological examination revealed that the anterior soft tissue to the implant exhibited a nonspecific foreign body reaction with connective tissue embed the biomaterials. Carbon fragment were seen in the surrounding tissue and some of the debric were phagocytosed by foreign body giant cell. Histological examination of bone and material revealed that new bone grew along the hole of CFR/PEEK implant. Conclusion: CFR/PEEK has an excellent biocompatibility to bone tissue.

Objective To investigate burnout and stress level and their correlation of nurses at class two psychiatric hospital in Beijing.Methods Burnout Inventory ( MBI ) and work stress scale were used to survey 119 psychiatric nurses at two class two psychiatric hospitals in Beijing.Results Both burnout and stress sources of nurses with different general information showed no statistic difference (P ＞ 0.05).Psychiatric nurses'burnout scores were lower than the dimension of MBI norm ( P ＜0.01).Burnout and emotional exhaustion scores,total scores and the dimensions of nursing stress were significantly positively correlated (P ＜ 0.05 or P ＜ 0.01).The tendency to depersonification was significantly positively correlated with other four items except the workload and time allocation ( P ＜ 0.05 or P ＜ 0.01).Personal sense of achievement was only negatively significantly associated with management and interpersonal relationship ( r =- 0.323,P ＜ 0.01).Sources of work stress of psychiatric nurses in 5 dimensions from high to low is working environment and resources (2.43 ± 0.921),the nursing profession and work ( 2.42 ± 0.839 ),workload and time allocation ( 2.40 ± 0.892 ),patients care (2.37 ±0.673 ),management and interpersonal relationship ( 1.82 ± 0.708 ).Conclusions Hospital managers is recommended to take effective measures to reduce or eliminate sources of stress of nurses,emphasis on the training of coping with the working burnout,relieve the nurses working burnout,and enhance the caring quality.

Adult ; Aged ; Female ; Fluid Therapy ; methods ; Humans ; Intensive Care Units ; Male ; Middle Aged ; Pancreatitis ; mortality ; therapy ; Plasma ; Young Adult

Growth suitability as assessment indicators for medicinal plants cultivation was proposed based on chemical quality determination and ecological factors analysis by Maxent and ArcGIS model. Notopterygium incisum, an endangered Chinese medicinal plant, was analyzed as a case, its potential distribution areas at different suitability grade and regionalization map were formulated based on growth suitability theory. The results showed that the most suitable habitats is Sichuan province, and more than 60% of the most suitable areawas located in the western Sichuan such as Aba and Ganzi prefectures for N. incisum. The results indicated that habitat altitude, average air temperature in September, and vegetation types were the dominant factors contributing to the grade of plant growth, precipitation and slope were the major factors contributing to notopterol accumulation in its underground parts, while isoimperatorin in its underground parts was negatively corelated with precipitation and slope of its habitat. However, slope as a factor influencing chemical components seemed to be a pseudo corelationship. Therefore, there were distinguishing differences between growth suitability and quality suitability for medicinal plants, which was helpful to further research and practice of cultivation regionalization, wild resource monitoring and large-scale cultivation of traditional Chinese medicine plants.
Apiaceae ; growth & development ; China ; Ecosystem ; Environment ; Geographic Information Systems ; Soil ; chemistry ; Temperature

OBJECTIVETo compare the effects of different kind of methods in the management of hydrofluoric acid burn in early postburn stage in rabbits.

METHODSThirty-three rabbits were inflicted with burn by 55% of hydrofluoric acid covering 5% TBSA, and were randomly divided into 3 groups, i.e. A (n = 13, with 5 ml.kg(-1).h(-1)of isotonic saline intravenous infusion), B (n = 10, with isotonic saline and 50 g/L of calcium gluconate infusion in dose of 20 mg/kg at different time points), and C (n = 10, with the same treatment as B group, and with excision of burn wound at 0.5 post burn hour) groups. The serum levels of fluorine and calcium were determined before and after various postburn hours, and the mortality rate was statistically analyzed.

RESULTS(1) The serum level of fluorine in A (8.37 +/- 2.62 mg/L) and B (8.59 +/- 2.25 mg/L) groups reached the peak value at 1 postburn hour (PBH), which was 107 times higher than that before the burn injury. The serum level of fluorine in B group was significantly lower than that in A group at 24 PBH (P < 0.05), while that in C group declined to (6.20 +/- 0.23) mg/L, which was obviously lower than that in A and B groups (P < 0.01). (2) The serum calcium level declined after burns, reaching the lowest level at 8 to 12 PBH. and began to increase at 24 PBH. Compared with normal calcium value, the serum level of calcium in A, B and C groups declined to as much as 46%, 32% and 26%, respectively. Statistically significant difference was found between C and B groups (P < 0.01). (3) The mortality rate in the three groups within 72 PBH were 30.8%, 12.5% and 0.0%, respectively.

CONCLUSIONEarly removal of burn area and calcium supplementation could help quickly decrease blood fluorine, reverse the fatal hypocalcemia and the multiple systemic toxic injury in rabbits inflicted with hydrofluoric acid injury.

Animals ; Burns, Chemical ; drug therapy ; surgery ; Calcium ; blood ; Calcium Gluconate ; therapeutic use ; Disease Models, Animal ; Elective Surgical Procedures ; Fluorine ; blood ; Hydrofluoric Acid ; adverse effects ; Rabbits ; Random Allocation ; Skin Transplantation ; Wound Healing

Since Taiwan is an endemic area for tuberculosis (TB), differential diagnosis between the intestinal TB and Crohn's disease is an important issue. The steering committee of Taiwan Society of Inflammatory Bowel Disease (TSIBD) has arranged a seminar accordingly on May 24th, 2014 and the different point of views by gastroenterologist, radiologist, pathologist and infectious disease specialist were suggested to help the proper diagnosis and management of these two diseases.
Communicable Diseases ; Crohn Disease* ; Diagnosis ; Diagnosis, Differential ; Inflammatory Bowel Diseases* ; Specialization ; Taiwan* ; Tuberculosis*

OBJECTIVETo investigate the effects of surgical treatment and prognosis of aneurysmal bone cyst (ABC) in mobile spine.

METHODSA total of 12 patients with ABC were operated on from 1996 to 2006, and the clinical data were retrospectively reviewed. The patients included 7 male and 5 female, aged from 16 to 52 years (mean, 29 years). Surgical interventions were selected according to WBB criteria. Seven patients underwent total spondylectomy, four underwent resection of posterior arch, one patient received sagittal resection only. Anti-poster or post-lateral approach reconstruction with bone-graft or bone cement and transpedicular screws fixation were performed in the cases. Eight cases received radiotherapy after the operation.

RESULTSThe mean operation blood lose was 3210 ml. The patients were followed-up for 10 to 116 months (mean, 41.8 months). Seven patients got complete recover of spinal cord function, 4 patients experienced local recurrence in 1-2 years post operation. One patient died of multiple metastasis of chondrosarcoma after radiotherapy.

CONCLUSIONSABC in spine is an aggressive disease with high local recurrence rate. Enbloc if possible provides the best result, with excellent prognosis. Radiotherapy should be selected carefully.

Adolescent ; Adult ; Bone Cysts, Aneurysmal ; diagnostic imaging ; surgery ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Prognosis ; Radiography ; Retrospective Studies ; Spinal Diseases ; diagnostic imaging ; surgery ; Treatment Outcome

Midkine is a heparin-binding growth factor, which plays important roles in the regulation of cell growth and differentiation. The non-tagged recombinant human midkine (rhMK) is therefore required to facilitate its functional studies of this important growth factor. In the present work, rhMK was expressed in Escherichia coli (E. coli) BL21 (DE3). The expression of midkine was efficiently induced by isopropyl-beta-D-thiogalactopyranoside (IPTG). After sonication, midkine was recovered in an insoluble form, and was dissolved in guanidine hydrochloride buffer. Renaturation of the denatured protein was carried out in the defined protein refolding buffer, and the refolded protein was purified using S-Sepharose ion-exchange chromatography. The final preparation of the rhMK was greater than 98% pure as measured by sodium dodecylsulfate-polyacrylamid gel electrophoresis (SDS-PAGE) and reverse phase high performance liquid chromatography (RP-HPLC). The purified rhMK enhanced the proliferation of NIH3T3 cells.
Animals ; Base Sequence ; Cell Proliferation ; drug effects ; Cytokines ; biosynthesis ; genetics ; isolation & purification ; pharmacology ; Escherichia coli ; genetics ; Humans ; Mice ; Molecular Sequence Data ; NIH 3T3 Cells ; Recombinant Proteins ; biosynthesis ; isolation & purification

Adolescent ; Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Diabetes Mellitus ; epidemiology ; etiology ; Female ; Glucose Intolerance ; epidemiology ; Glucose Tolerance Test ; Humans ; Hypertension ; complications ; Male ; Mass Screening ; Middle Aged ; Obesity ; complications ; Prevalence ; Rural Health ; Sampling Studies ; Surveys and Questionnaires ; Urban Health