1.Phenolic glycosides from the stems and twigs of Strychnos cathayensis and their biological activities
Qi-ming PAN ; Shuang-gang MA ; Yong LI ; Dan ZHANG ; Hua SUN ; Qi HOU ; Xiao-guang CHEN ; Shi-shan YU
Acta Pharmaceutica Sinica 2023;58(1):170-179
Fourteen compounds were isolated from the
3.Gene expression data classification using consensus independent component analysis.
Chun-Hou ZHENG ; De-Shuang HUANG ; Xiang-Zhen KONG ; Xing-Ming ZHAO
Genomics, Proteomics & Bioinformatics 2008;6(2):74-82
We propose a new method for tumor classification from gene expression data, which mainly contains three steps. Firstly, the original DNA microarray gene expression data are modeled by independent component analysis (ICA). Secondly, the most discriminant eigenassays extracted by ICA are selected by the sequential floating forward selection technique. Finally, support vector machine is used to classify the modeling data. To show the validity of the proposed method, we applied it to classify three DNA microarray datasets involving various human normal and tumor tissue samples. The experimental results show that the method is efficient and feasible.
Artificial Intelligence
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Colonic Neoplasms
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classification
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genetics
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Computational Biology
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Data Interpretation, Statistical
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Databases, Genetic
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Discriminant Analysis
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Gene Expression Profiling
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statistics & numerical data
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Glioma
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classification
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genetics
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Humans
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Leukemia
;
classification
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genetics
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Models, Statistical
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Neoplasms
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classification
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genetics
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Oligonucleotide Array Sequence Analysis
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statistics & numerical data
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Principal Component Analysis
4.A study of paternity testing with considering mutation.
Yi-ping HOU ; Jin WU ; Jia-yi HOU ; Jin YAN ; Ying-bi LI ; Shuang GAO ; Xiao-ming SUN ; Hai-bo LUO
Chinese Journal of Medical Genetics 2007;24(4):417-421
OBJECTIVETo formulate recommendations in the evaluation of results of genetic analyses in paternity testing under considering mutations.
METHODSA total of 15 short tandem repeat(STR) loci were employed for this study, which were included CSF1PO, FGA, TH01, TPOX, VWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, PentaD and PentaE. Both 100 cases of true trio and 100 cases of false trio were investigated.
RESULTSThe numbers of mismatch alleles in different STR loci were observed in 100 cases of false trio. The different distributions of paternity index were obtained, including the changes of paternity index in each case of true trio under simulated mutations.
CONCLUSIONIn order to avoid the effect of mutations, the exclusion of paternity was never considered on the basis of a single locus. The threshold values of the combined probability of exclusion and the paternity index were important for both exclusion and inclusion of paternity. The scientific evidence for paternity testing can be obtained when both the combined probability of exclusion and the paternity index meet the threshold values. However, when either the combined probability of exclusion or the paternity index can not meet the threshold values, more genetic markers should be added.
Female ; Forensic Genetics ; methods ; Humans ; Male ; Microsatellite Repeats ; genetics ; Mutation ; Nuclear Family ; Paternity ; Reproducibility of Results
5.Neuroprotective effects and mechanisms of Cerebroprotein Hydrolysate for Injection (Ⅰ) on vascular dementia in rats
long Xiao JING ; Li MA ; ming Lei REN ; bao Yan HOU ; Yan HUANG ; yong Shuang SUN ; Jing LIU ; ping Xiu SHEN
Drug Evaluation Research 2017;40(8):1078-1081
Objective To investigate the neuroprotective mechanisms of Cerebroprotein Hydrolysate for Injection (Ⅰ) on vascular dementia in rats.Method The rat vascular dementia model was prepared using an improved two-vessel occlusion method,and the common carotid artery was only isolated but not blocked in sham group.Rats were randomly divided into sham group,model group,Cerebroprotein Hydrolysate for Injection (Ⅰ) groups with low,medium and high dose (5,10,20 mg/kg) and Cerebroprotein Hydrolysate Injection group (Cerebrolysin,Positive drug,10 mg/kg).The drug was administered by iv injection of rat tail vein once a day for two weeks,while the same volume of saline was administered in sham and model group.At the end of administration,the plasma was collected through abdominal aorta to separate serum,and rat cortex was isolated to prepare homogenate.The levels of nerve growth factor (NGF) and insulin-like growth factor 2 (IGF-2) in serum and level of gamma-aminobutyric acid (GABA) in cortex were detected by ELISA.Level of glutamate (Glu) in cortex of VaD rats was detected by colorimetry.Results Compared with model group,levels of NGF and IGF-2 in the serum of VaD rats and level of GABA in cortex were significantly increased,while level of Glu in cortex was significantly decreased after administration of Cerebroprotein Hydrolysate for Injection (Ⅰ).The increased IGF-2 and GABA levels by Cerebroprotein Hydrolysate for Injection (Ⅰ) were significantly higher than that of Cerebrolysin at same dose.Conclusion The mechanisms underlying the increased leaming and memory ability of VaD rats by Cerebroprotein Hydrolysate for Injection (Ⅰ),are possibly related to the increased levels of NGF and IGF-2 in body and a regulation of the balance between excitatory and inhibitory amino acid neurotransmitters.
6.Clinical application of extracorporeal membrane oxygenation for treatment of adult refractory cardiogenic shock.
Liu-sheng HOU ; Gang XIE ; Jian-wei LI ; Chong-hui JIANG ; Yong YUAN ; Bin-fei LI ; Hai-ming JIANG ; Ye NING ; Gui-shen WU ; Wei-hua ZHENG ; Shuang-biao ZHAO
Chinese Journal of Cardiology 2013;41(7):590-593
OBJECTIVETo summarize the clinical experience of extracorporeal membrane oxygenation (ECMO) treatment for adult refractory cardiogenic shock.
METHODSFrom January 2003 to January 2011, patients with refractory cardiogenic shock required veno-arterial ECMO by failure of conventional therapy and intra-aortic balloon pump counterpulsation therapy were retrospectively studied. Patients with severe traumatic brain injury, advanced malignancies and multiple organ failure were excluded. Patients were divided into weaned group (n = 31) and not weaned group (n = 23) according to the ECMO weaning.
RESULTSThe duration of ECMO was 24.16 (14.12, 56.75) hours. Twenty-two out of 31 patients in the weaned group survived and were discharged, 9 patients died after successfully weaned from ECMO (5 due to multisystem organ failure, 2 due to reoccurred cardiogenic shock, 1 due to infectious shock and 1 due to disseminated or diffuse intravascular coagulation). Pre-ECMO mean arterial pressure, ejection fraction, the duration of ECMO were significantly higher while pre-ECMO blood lactate [(8.64 ± 3.17) vs. (14.44 ± 2.52) , P < 0.01], the duration of ROSC [ (16.70 ± 5.29) vs. (35.64 ± 5.89), P < 0.01] and multisystem organ failure [0 vs. 17.4% (4/23) , P < 0.05] were lower in weaned group than in not wean group.
CONCLUSIONSECMO is an effective mechanical assistant therapy strategy for adult refractory cardiogenic shock patients. Timely applying this strategy on suitable patients is crucial for the success of ECMO. Cardiac function and reversibility of heart failure are key factors determine the fate of weaned or not weaned ECMO in adult refractory cardiogenic shock patients.
Adult ; Extracorporeal Membrane Oxygenation ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Shock, Cardiogenic ; therapy
7.The Molecular Mechanism of LncRNA-CASC2/miR-155-5p/APC Axis Regulating the Progression of Non-Hodgkin Lymphoma.
Chun-Jiao ZHENG ; Zhi-Ming WU ; Yan-Shuang WANG ; Yun-Xiu HOU
Journal of Experimental Hematology 2020;28(6):1939-1945
OBJECTIVE:
To investigate the effect of lncRNA-CASC2 (CASC2) /miR-155-5p/APC axis to the progression of non-Hodgikn lymphoma (NHL).
METHODS:
The expression level of CASC2 and miR-155-5p in NHL cell lines were examined by qRT-PCR. Dual-luciferase reporter gene assay was used to verify the relationship between miR-155-5p, CASC2 and APC. The effects of CASC/miR-155-5p/APC axis to the proliferation, invasion and apoptosis of NK-92 cells were detected by MTT, Transwell assay and flow cytometry assay, respectively.
RESULTS:
CASC2 was downregulated in NHL cell lines. Overexpression of CASC2 could inhibit the proliferation and invasion of NK-92 cells, and promote its apoptosis. Dual-luciferase reporter gene assay confirmed that there was a targeting relationship between miR-155-5p, CASC2 and APC. The restoration experiments proved that knockdown of both miR-155-5p and CASC2 or APC could restore the inhibitory effect of miR-155-5p silencing to the biological behavior of NK-92 cells.
CONCLUSION
Overexpression of CASC2 suppresses the proliferation and invasion of NK-92 cells, promote the apoptosis of NK-92 cells via targeting miR-155-5p and upregulating APC expression.
Cell Line, Tumor
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Gene Expression Regulation, Neoplastic
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Humans
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Lymphoma, Non-Hodgkin/genetics*
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MicroRNAs/genetics*
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RNA, Long Noncoding/genetics*
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Tumor Suppressor Proteins/genetics*
8. Research progress of MicroRNA in hypertension and its target organ damage
Jing XU ; Qian-Qian CHEN ; Ming-Shuang HOU ; Hong-Ying LYU ; Guan-Jun JIA ; Yu-Shun KOU ; Lin YI ; Jing XU ; Qian-Qian CHEN ; Ming-Shuang HOU ; Hong-Ying LYU ; Guan-Jun JIA ; Yu-Shun KOU ; Lei ZHAO
Chinese Pharmacological Bulletin 2023;39(10):1824-1828
Hypertension is a risk factor for a variety of cardiovascular diseases, which is an important public health problem in the world today. MiRNAs are a class of highly conserved non-coding small RNAs. In recent years, studies have found that miRNAs are involved in the occurrence and development of hypertension through a variety of ways, causing damage to the important target organs of hypertension, such as heart, brain and kidney. This article reviews the research progress of miRNA in hypertension in recent years, in order to clarify its role in the process of hypertension and target organ damage, and provide ideas for exploring new therapeutic targets of hypertension.
9.Application of a novel porous tantalum implant in rabbit anterior lumbar spine fusion model: in vitro and in vivo experiments.
Ming LU ; Song XU ; Zi-Xiong LEI ; Dong LU ; Wei CAO ; Marko HUTTULA ; Chang-He HOU ; Shao-Hua DU ; Wei CHEN ; Shuang-Wu DAI ; Hao-Miao LI ; Da-Di JIN
Chinese Medical Journal 2019;132(1):51-62
BACKGROUND:
Some porous materials have been developed to enhance biologic fusion of the implants to bone in spine fusion surgeries. However, there are several inherent limitations. In this study, a novel biomedical porous tantalum was applied to in vitro and in vivo experiments to test its biocompatibility and osteocompatibility.
METHODS:
Bone marrow-derived mesenchymal stem cells (BMSCs) were cultured on porous tantalum implant. Scanning electron microscope (SEM) and Cell Counting Kit-8 assay were used to evaluate the cell toxicity and biocompatibility. Twenty-four rabbits were performed discectomy only (control group), discectomy with autologous bone implanted (autograft group), and discectomy with porous tantalum implanted (tantalum group) at 3 levels: L3-L4, L4-L5, and L5-L6 in random order. All the 24 rabbits were randomly sacrificed at the different post-operative times (2, 4, 6, and 12 months; n = 6 at each time point). Histologic examination and micro-computed tomography scans were done to evaluate the fusion process. Comparison of fusion index scores between groups was analyzed using one-way analysis of variance. Other comparisons of numerical variables between groups were made by Student t test.
RESULTS:
All rabbits survived and recovered without any symptoms of nerve injury. Radiographic fusion index scores at 12 months post-operatively between autograft and tantalum groups showed no significant difference (2.89 ± 0.32 vs. 2.83 ± 0.38, F = 244.60, P = 0.709). Cell Counting Kit-8 assay showed no significant difference of absorbance values between the leaching liquor group and control group (1.25 ± 0.06 vs. 1.23 ± 0.04, t = -0.644, P = 0.545), which indicated the BMSC proliferation without toxicity. SEM images showed that these cells had irregular shapes with long spindles adhered to the surface of tantalum implant. No implant degradation, wear debris, or osteolysis was observed. Histologic results showed solid fusion in the porous tantalum and autologous bone implanted intervertebral spaces.
CONCLUSION
This novel porous tantalum implant showed a good biocompatibility and osteocompatibility, which could be a valid biomaterial for interbody fusion cages.
Animals
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Cell Proliferation
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physiology
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Diskectomy
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Lumbar Vertebrae
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surgery
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Microscopy, Electron, Scanning
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Prostheses and Implants
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Rabbits
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Spinal Fusion
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Tantalum
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chemistry
10.Monitoring Value of Multimodal Magnetic Resonance Imaging in Disease Progression of Amyotrophic Lateral Sclerosis: A Prospective Observational Study.
Dong-Chao SHEN ; Yin-Yan XU ; Bo HOU ; Hong-Fei TAI ; Kang ZHANG ; Shuang-Wu LIU ; Zhi-Li WANG ; Feng FENG ; Ming-Sheng LIU ; Li-Ying CUI
Chinese Medical Journal 2018;131(24):2904-2909
Background:
Ongoing efforts have been made to identify new neuroimaging markers to track amyotrophic lateral sclerosis (ALS) progression. This study aimed to explore the monitoring value of multimodal magnetic resonance imaging (MRI) in the disease progression of ALS.
Methods:
From September 2015 to March 2017, ten patients diagnosed with ALS in Peking Union Medical College Hospital completed head MRI scans at baseline and during follow-up. Multimodal MRI analyses, including gray matter (GM) volume measured by voxel-based morphometry; cerebral blood flow (CBF) evaluated by arterial spin labeling; functional connectivity, including low-frequency fluctuation (fALFF) and regional homogeneity (ReHo), measured by resting-state functional MRI; and integrity of white-matter (WM) fiber tracts evaluated by diffusion tensor imaging, were performed in these patients. Comparisons of imaging metrics were made between baseline and follow-up using paired t-test.
Results:
In the longitudinal comparisons, the brain structure (GM volume of the right precentral gyri, left postcentral gyri, and right thalami) and perfusion (CBF of the bilateral temporal poles, left precentral gyri, postcentral gyri, and right middle temporal gyri) in both motor and extramotor areas at follow-up were impaired to different extents when compared with those at baseline (all P < 0.05, false discovery rate adjusted). Functional connectivity was increased in the motor areas (fALFF of the right precentral gyri and superior frontal gyri, and ReHo of right precentral gyri) and decreased in the extramotor areas (fALFF of the bilateral middle frontal gyri and ReHo of the right precuneus and cingulate gyri) (all P < 0.001, unadjusted). No significant changes were detected in terms of brain WM measures.
Conclusion
Multimodal MRI could be used to monitor short-term brain changes in ALS patients.
Adult
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Amyotrophic Lateral Sclerosis
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diagnostic imaging
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physiopathology
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Brain
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diagnostic imaging
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Cerebrovascular Circulation
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Disease Progression
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Female
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Humans
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Magnetic Resonance Imaging
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methods
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Male
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Middle Aged
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Multimodal Imaging
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methods
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Prospective Studies