1.Comparative study between MEWS score, APACHE Ⅱ score and combination of the two scoring systems to predict the emergency medicine prognosis
Ping LI ; Lan DING ; Tingting WEI ; Ming HOU
Chinese Journal of Practical Nursing 2015;31(3):166-168
Objective We sought to compare the ability of MEWS score,APACHE Ⅱ score and combination of the two scoring systems to predict the prognosis of patients in emergency medical department.Methods During January to March 2014,640 patients in emergency medical department who met the criteria were set as the research object.The patients admission was the starting point for clinic observation.The relevant data were collected for carrying on the MEWS and APACHE Ⅱ ratings,tracking the patients' prognosis.The corresponding predictors for prognosis of patients such as sensitivity,specificity,positive predictive value,negative predictive value and ROC curve by MEWS score,APACHE Ⅱ score and combination of the two scoring systems were compared.Results The area under the receiver operating characteristic curve was 0.93,0.79 and 0.93 with MEWS score,APACHE Ⅱ score and combination of the two scoring systems.The comparison of either of the two scoring systems showed significant difference.When death was named as the prediction factor,the sensitivity,specificity,positive predictive value and negative predictive value were 76.92%,91.70%,51.02%,97.23% for MEWS score; 83.08%,62.80%,20.15% and 97.04% for APACHE Ⅱ score; 92.31%,86.43%,43.48% and 97.58% for combination of the two scoring systems.Conclusions Combination of the MEWS and APACHE Ⅱ scoring systems can be used to predict the prognosis of patients in emergency medical department.It posesses high sensitivity,specificity,positive predictive value and negative predictive value,which indicating a high predictive capability.
2.Analysis and evaluation of nutritive elements in aerial part of Panax notoginseng.
Yuan QU ; Ying LIU ; Lu-Qi HUANG ; Lan-Ping GUO ; Xiu-Ming CUI
China Journal of Chinese Materia Medica 2014;39(4):601-605
OBJECTIVETo make full use of the plant resources of Panax notoginseng, nutritional compositions and mineral elements were analyzed in aerial part of P. notoginseng from different areas in Yunnan.
METHODUsing the national standard method, water, ash, crude fat, crude fiber, crude protein and mineral elements were determined in aerial part of P. notoginseng from different growing areas.
RESULTResults showed that there were higher contents of crude fiber and crude protein, and lower content of crude fat in the stems and flowers of P. notoginseng. Meanwhile, a large number of mineral elements were determined in two locations of P. notoginseng, and the contents of Zn, Fe, Mn, Ca and Mg were obvious higher among these mineral elements.
CONCLUSIONThis study showed that the stems and flowers of P. notoginseng were nutritious and suggested that the aerial part may be utilized as new resources foods.
Flowers ; chemistry ; Nutritive Value ; Panax notoginseng ; chemistry ; Plant Extracts ; analysis ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Trace Elements ; analysis
3.Chinese Sanqi industry status and development countermeasures.
Xiu-Ming CUI ; Lu-Qi HUANG ; Lan-Ping GUO ; Da-Hui LIU
China Journal of Chinese Materia Medica 2014;39(4):553-557
Sanqi (Panax notoginseng) is a valuable unique herb, and is also one of the very fast developed varieties of traditional Chinese medicines in recent years with increasing role in traditional Chinese medicine industry. This paper summarized the main experience, industry development and present situation, pointed out the main problems existing in the industry development. On this basis, we put forward the targets and measures for the development of the Sanqi industry in to provide decision-making reference for the sustainable development of the Sanqi industry in China.
China
;
Drug Industry
;
economics
;
trends
;
Drugs, Chinese Herbal
;
analysis
;
economics
;
Medicine, Chinese Traditional
;
economics
;
trends
;
Panax notoginseng
;
chemistry
;
growth & development
4.Study of biomechanical properties of mucosa scars after cleft palate surgery.
Min HOU ; Guang-Yu SHI ; Wei QIU ; Lan-Cheng ZHANG ; Tian-Ping YU ; Chun-Ming LIU
Chinese Journal of Plastic Surgery 2013;29(6):453-456
OBJECTIVETo explore biomechanical properties and stress-strain of mucosa scars after cleft palate surgery.
METHODSAfter the model of mucosa scars was made, the mucosa scars and normal mucosa were excised and examined immediately by tensionometry.
RESULTSThe mucosa scars after cleft palate surgery were compared with normal mucosa. The Poisson's ratio of mucosa scars and normal mucosa was 0.5 and 0.49, respectively, showing no significant difference between the two groups. The ultimate Young's modulus of mucosa scars was about 24.22 MPa, however, it declined to 3.32 Mpa in normal mucosa.
CONCLUSIONSThe mucosa scars after cleft palate surgery are biomechanically weaker than normal mucosa. It can be used for further research, such as maxillary orthognathic surgery, distraction osteogenesis, and orthodontic treatment.
Biomechanical Phenomena ; Cicatrix ; physiopathology ; Cleft Palate ; surgery ; Humans ; Mouth Mucosa ; physiopathology ; surgery ; Osteogenesis, Distraction ; Osteotomy, Le Fort
5.Comprehensive chemical pattern recognition of atractylodis rhizoma.
Fan WANG ; Zhen OUYANG ; Lan-Ping GUO ; Ming ZHAO ; Hua-Sheng PENG ; Jing-Lin LIAO ; Zhong-Ping LIANG
China Journal of Chinese Materia Medica 2014;39(13):2536-2541
A method of comprehensive chemical pattern recognition of Atractylodis Rhizoma was established by GC-MS fingerprint, principal component analysis, cluster analysis and discriminant analysis. A DB-wax column (0.25 mm x 60 m, 0.25 microm) with El ion source and 70 V electron multiplier were used for GC-MS analysis. Using principal component analysis, cluster analysis, and discriminant analysis, 15 common peaks of sample fingerprints for chemical pattern recognition research were analysed. The same results were obtained from the fingerprint, principal component analysis and cluster analysis, which could use to distinguish genuine Atractylodes lancea, ungenuine A. lancea and A. chinensis. Thus, this method could be used for the quality control and comprehensive evaluation of Atractylodis Rhizoma.
Atractylodes
;
chemistry
;
China
;
Discriminant Analysis
;
Drugs, Chinese Herbal
;
chemistry
;
Gas Chromatography-Mass Spectrometry
;
methods
;
Quality Control
;
Rhizome
;
chemistry
6.ShRNA-mediated gene silencing of beta-catenin inhibits growth of human colon cancer cell Colo205 in vitro.
Wen-Sheng HUANG ; Hui PENG ; Ming TAN ; Jin-Ping MA ; Jian-Ping WANG ; Ping LAN
Chinese Journal of Gastrointestinal Surgery 2009;12(3):301-305
OBJECTIVETo observe the gene silencing and disruption of WNT pathway mediated by the specific shRNA targeted against beta-catenin and its effect on cell proliferation of the human colon cancer cell line Colo205.
METHODSThe shRNA plasmid vectors against beta-catenin were constructed and transfected into Colo205 cells with Lipofectamine 2000. The expression of beta-catenin was detected by RT-PCR and Western blot. Immunofluorescence staining was also performed to detect the beta-catenin protein expression in cells. The cell proliferation inhibition was determined by MTT assay and soft agar colony formation assay.
RESULTSThe shRNA vectors targeted against beta-catenin were successfully constructed and efficiently suppressed the expression of beta-catenin mRNA and protein(P<0.05). The expression inhibition rates were 47.89% and 45.26% at the mRNA and protein level respectively. Immunofluorescence microscopy also demonstrated the inhibition of beta-catenin protein induced by these specific shRNAs. The MTT assay indicated that the specific shRNA resulted in significant inhibition of cell growth on the culture plates in time-dependent manner. At 72 h post-transfection, the cell viability of CAT group was 48.5%, which was significantly different as compared with that of blank control group's 91.3%(P<0.05). In the soft agar, there were 9, 46, 43 cell colonies in the CAT, blank, and negative control groups respectively, which were significantly different(P<0.05).
CONCLUSIONSThe specific shRNAs targeted against beta-catenin has a gene silencing effect and blocks the WNT signaling pathway, which can inhibit the growth of Colo205 cells.
Cell Line, Tumor ; Cell Proliferation ; Colonic Neoplasms ; genetics ; metabolism ; Humans ; In Vitro Techniques ; RNA Interference ; Signal Transduction ; Transfection ; Wnt Proteins ; metabolism ; beta Catenin ; genetics
7.A study on quasispecies of HBV precore in liver tissue and serum in fulminant hepatitis B patients.
Xue-lan ZHAO ; Yu-ming WANG ; Lin LAN ; Yan-ping HUANG
Chinese Journal of Hepatology 2005;13(8):612-613
Adult
;
DNA, Viral
;
genetics
;
Hepatitis B virus
;
genetics
;
physiology
;
Hepatitis B, Chronic
;
virology
;
Humans
;
Liver
;
virology
;
Male
;
Severity of Illness Index
;
Viral Core Proteins
;
genetics
;
Virus Replication
8.Study on dynamic change of middle and micro element in Panax notoginseng plant soils with different interval year.
Ye YANG ; Li WANG ; Lan-Ping GUO ; Xiu-Ming CUI ; Hang JIN ; Xin-Yan ZHU ; Da-Hui LIU
China Journal of Chinese Materia Medica 2014;39(4):580-587
OBJECTIVETo study effects of different interval year on Panax notoginseng plant soils middle and micro element content.
METHODThe dynamic change of Ca, Mg, Mn, Cu, Zn, B from Mabai, Matang, Gumu and Panlong were determined under different planting patterns (new soil, interval 5 years soil and continuous cropping soil).
RESULTAll the micro elements (except Ca, Mg) of interval 5 years soil and continuous cropping soil were significantly higher than those of new soil. All the middle and micro elements (except B) of interval 5 years soil were significantly higher than those of the continuous cropping soil. Planting patterns had remarkable influence on the content of Mn, Cu, B, but not Zn Ca, Mg. Cu, Ca under the 3 planting patterns, and Zn under the continuous cropping pattern did not show significant quarter changes. B content increased with the elongation of implantation time. Zn in new soil and interval 5 years also increased with prolonging of planted time. Mg, Mn and Cu content reached to peak value on April next year, and reached to minimum on the end of this experiment. Compared with new soil, the proportion of Mn, Cu in total elements increased by 29%, 114%, Mg, B decreased by 18%, 38%, Zn and Ca changed slightly of interval 5 years soils; In continuous cropping soil, Mn, Cu and B increased by 50%, 120%, 22%, respectively, but Zn, Ca, Mg had no significant change.
CONCLUSIONContinuous cropping pattern could not induce the deficient of soil middle and micro elements, and thereafter might not result in continuous cropping obstacles. But the imbalance proportional of soil middle and micro elements in P. notoginseng plant soils may be one of the main reasons for continuous cropping obstacles.
China ; Kinetics ; Panax notoginseng ; growth & development ; Soil ; chemistry ; Time Factors ; Trace Elements ; chemistry
9.Study on dynamic change law of N, P and K in Panax notoginseng plant soils with different interval year.
Da-Hui LIU ; Li WANG ; Xiu-Ming CUI ; Lan-Ping GUO ; Hang JIN ; Xin-Yan ZHU ; Ye YANG
China Journal of Chinese Materia Medica 2014;39(4):572-579
OBJECTIVETo study effects of different interval year on Panax notoginseng plant soils macro element content.
METHODThe dynamic change of total N, P, K and available N, P, K in soil from Mabai, Matang, Gumu and Panlong was determined under different planting patterns (new soil, interval 5 years soil and continuous cropping soil).
RESULTContents order of soil total N, P and available N, P were interval 5 years soil > continuous cropping soil > new soil. No significant quarter change on soil total N was found, but the other three showed inverted "v" curve, and the peak value appeared on April 2010. Content of soil total K did not change significantly, but the available K content order was new soil > continuous cropping soil > interval 5 years soil, the quarter change was similar as soil available N or P. The soil total N, P, K and available N, P, K were different of the 4 monitoring sites under the 3 interval planting modes. There was a significant correlation between soil total P and available P under all these 3 interval planting modes, but N and K. The propitiation of N-P-K of new soil, interval 5 years soil and continuous cropping soil were 1: 0.4: 2.4, 1: 0.4:1.4, 1:0. 4:2.0, respectively.
CONCLUSIONContinuous cropping pattern induce the accumulation of P, but deficient of K. The imbalance proportion of N, P and K was one of the incentives of continuous cropping induced obstacles. Strengthen the research of optimum proportion of soil N, P and K, and then eliminate continuous cropping obstacles by means of formulated fertilization is the future research direction.
Agriculture ; methods ; Breeding ; China ; Drugs, Chinese Herbal ; analysis ; Fertilizers ; analysis ; Nitrogen ; analysis ; metabolism ; Panax notoginseng ; chemistry ; growth & development ; metabolism ; Phosphorus ; analysis ; metabolism ; Potassium ; analysis ; metabolism ; Quality Control ; Soil ; chemistry
10.Preliminary study on suitability of ozone sterilization in traditional Chinese medicine and its preparation.
Yan-jun HU ; Ya-qi WANG ; Zhen-feng WU ; Ji-ping LAN ; Li-guo ZHANG ; Ming YANG
China Journal of Chinese Materia Medica 2015;40(16):3137-3141
Microbial contamination, growth and reproduction have a great influence on the quality of traditional Chinese medicine (TCM) and its preparation. TCM may be polluted by microbial in the production process due to ambient air, facilities and appliances and operating personnel, which affects the quality and efficacy of the final product. The GMP certification inspection standard of TCM manufacturing enterprises clearly established: "Sterilization methods of Chinese herbal medicine, intermediate products, finished products should follow the principle whether or not to change its quality", "TCM powder that used as medicine directly should follow the principle whether to do microbiological examinations". So it's particularly important for product quality, corporate energy consumption and its efficiency to choose the scientific and effective sterilization techniques and methods. Ozone is a kind of safe, environmentally friendly, efficient and no residue emerging sterilization technology. It has been widely applied in various fields of medical and health care and production and living. This paper mainly analyzed the ozone sterilization technology of TCM, aiming to explore the principle of ozone sterilization, the advantages, application status and existing problems and so on. The management regulations and implementation rules of ozone sterilization were summarized to make sterilization of Chinese medicine in the production, management, quality control and other aspects standardized, reasonable and scientific, and to provide the theoretical reference of the ozone sterilization technology for TCM and its preparation.
Bacteria
;
drug effects
;
growth & development
;
Drug Contamination
;
prevention & control
;
Drugs, Chinese Herbal
;
chemistry
;
Humans
;
Ozone
;
pharmacology
;
Plants, Medicinal
;
microbiology
;
Sterilization
;
methods