1.The influence of silencing miRNA-155 on proliferation and apoptosis of human cutaneous squamous cell carcinoma cell line A431
Tianjin Medical Journal 2017;45(9):902-906
Objective To investigate the effects of antisense oligonucleotide against miRNA-155 (AS-miRNA-155) on proliferation,apoptosis and invasion and migration abilities of human cutaneous squamous cell carcinoma cell line A431. Methods AS-miRNA-155 was transfected into human cutaneous squamous cell carcinoma A431 cells by using LipofectamineTM 2000. Blank control without transfection and transfected with non-sense sequence were used as non-sense sequence control. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miRNA-155 in A431 cells. Cell proliferation was analyzed using dimethyl thiazolyldiphenyl tetrazolium (MTT) assay. Cell cycle arrest and apoptosis were studied by flow cytometry (FCM). Invasion and migration were measured by Transwell chamber assays. Results The relative expression of miRNA-155 mRNA was lower in the transfection group than that in the blank control group and the negative control group (F=634.57, P<0.01), but there was no significant difference between the blank control group and the negative control group. After 72 h transfection, the survival rate was significantly lower in the transfection group than that of the blank control group and the negative control group, and the transfection rate decreased significantly by 120 h (P<0.05). Cells of G0/G1 phase increased, Cells of S phase reduced, the overall PI value decreased in transfection group, and the apoptosis rate of A431 cells, migration and invasion of cells increased (P<0.05). There was no significant difference in G2/M cycle between transfection group, blank control group and negative control group. There were no significant differences in A431 cell apoptosis rate, cell migration and invasive ability between blank control group and negative control group. Conclusion Antisense oligonucleotide against miRNA-155 can inhibit the expression of miRNA-155, the proliferation and promote the apoptosis of human cutaneous squamous cell carcinoma A431 cells, which indicates that miRNA-155 may become a new target for the regulation of gene expression in cutaneous squamous cell carcinoma.
2.Progress on cervical muscle strength and soft tissue stiffness testing.
China Journal of Orthopaedics and Traumatology 2015;28(8):771-775
Biomechanical evaluation of neck muscles has important significance in the diagnosis and treatment for cervical spondylosis, the neck muscle strength and soft tissue stiffness test is two aspects of biomechanical testing. Isometric muscle testing operation is relatively simple, the cost is lower, which can evaluate the muscle force below grade 3. However, isokinetic muscle strength testing can assess the muscle strength of joint motion in any position. It is hard to distinguish stiffness difference in different soft tissues when the load-displacement curve is used to evaluate the local soft tissue stiffness. Elasticity imaging technique can not only show the elastic differences of different tissues by images, but also quantify the elastic modulus of subcutaneous tissues and muscles respectively. Nevertheless, it is difficult to observe the flexibility of the cervical spine by means of the analysis of the whole neck stiffness. In a word, a variety of test method will conduce not only the biomechanical evaluation of neck muscles, but also making an effective biomechanics mathematical model of neck muscles. Besides, isokinetic muscle testing and the elasticity imaging technology still need further validation and optimization before they are better applied to neck muscles biomechanical testing.
Biomechanical Phenomena
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Humans
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Muscle Strength
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Neck Muscles
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physiology
7.Application of Internal Amplification Control in the PCR Detection Method for Food-borne Salmonella
Bin LIU ; Xian-Ming SHI ;
Microbiology 1992;0(02):-
An internal amplification control, which could be co-amplified with the invA target gene of Salmonella in the PCR system, was constructed in order to indicate possible PCR inhibitors derived from food samples. Specificity of this PCR system was tested with 9 Salmonella strains and 15 non-Salmonella strains, and the results showed that there was a 374 bp amplicon resulted from all Salmonella strains, while only a 513 bp IAC amplicon appeared after the amplification for all non-Salmonella strains. The detection sensitivity of this PCR system was 12.8 fg/?L for purified target DNA, and the detection limit for artificially inoculated milks was 8 cfu /25g if they were enriched for 8h in buffered peptone water. Salmonella in 80 samples of seriously contaminated milks was detected by the PCR method developed in this study, and the experiments demonstrated that it could successfully eliminate false-negative results.
8. Bioequivalence study of paroxetine hydrochloride film-coated tablets
Chinese Pharmaceutical Journal 2012;47(5):367-370
OBJECTIVE: To evaluate the relative bioavailibility and bioequivalence of paroxetine hydrochloride film-coated tablets. METHODS: In a randomized crossover study, 24 healthy Chinese male subjects received a single oral dose (20 mg) of either test or reference paroxetine hydrochloride tablets after an overnight fast. The plasma concentrations of paroxetine were determined by a validated LC-MS/MS method. The pharmacokinetic parameters, the relative bioavailability and bioequivalence of two formulations were evaluated by DAS 2.0 software. RESULTS: After a single oral dose of 20 mg test or reference paroxetine tablets, the pharmacokinetic parameters of paroxetine were as follows: ρmax(5.102 ± 2.955) and (5.396 ± 2.852) μg · L-1; tmax (5.22 ± 1.83) and (5.35 ± 0.78) h; t1/2(11.76 ± 2.91) and (11.98 ± 3.57) h; AUC0~96h(118.1 ± 90.2) and (118.9 ± 86.0) μg · h · L-1; AUC0-∞ (120.2 ± 91.0) and (121.5 ± 87.6) μg · h · L-1, respectively. CONCLUSION: The relative bioavailability of the test paroxetine hydrochloride film-coated tablets is (100.6 ± 22.0)%. The two preparations are bioequivalent. Copyright 2012 by the Chinese Pharmaceutical Association.
9.The Role of Selectins in Ischemia-Reperfusion Injury of Brain
International Journal of Cerebrovascular Diseases 2006;0(11):-
Selectins are a family of adhesion molecules,including P-,L-,E-selectins.The three adhesion molecules all participate in the inflammatory processes of ischemia-reperfusion injury of brain.P-selectin is expressed on activated platelets as well as on endothelial cells.E-selectin is only expressed on endothelial cells.P-and E-selectin mediate the adhesion of the leukocytes,platelets and endothelial cells.L-selectin is mainly expressed on leukocytes and mediates leukocytes rolling contact with microvascular endothelial cells.