1.Effect of Naoxueshu oral liquid on cerebral hemorrhage caused by hypertension
Ming LU ; Ru-ping XIE ; Yu FU ; Daozhen XIE
Chinese Journal of Rehabilitation Theory and Practice 2004;10(5):304-305
ObjectiveTo investigate the effect of Naoxueshu oral liquid on cerebral hemorrhage caused by hypertension. Methods140 patients with cerebral hemorrhage were randomly dividied into two groups with a 1∶1 ratio,treatment group and control group. Patients in treatment group received Naoxueshu oral liquid 30 ml daily,while that in control received Naoxuekang oral liquid 30 ml daily. The changes of score on Chinese medical integration,National Institutes of Health (NIH) Stroke scale and the volume of intracranial hemorrhage were evaluated before and a month after treatment.ResultsThe decrease of NIH score of patients in treatment group was (12.73±3.94),but(4.72±3.01) with the control's (t=13.5327,P<0.001). The decrease of the volume of intracranial hemorrhage of patients in treatment group was (13.28±4.17) ml,but (8.37±7.24) ml with the control's(t=4.9228,P<0.001). ConclusionComparing with Naoxuekang,Naoxueshu can accelerate absorption of hematome and ameliorate the recovery of neurological disability more efficiently.
2.Fermentative technology of Soybean Isofiavone Glucoside Hydrolase-Producing Strain
Ming-Jie XIE ; Chun-Hua XU ; Chang-Jiang LIU ; Ming-Chun LU ; Feng-Xie JIN ;
Microbiology 1992;0(03):-
A high active soybean isoflavone glucoside hydrolase-producing mould strain was isolated from spirit qu. Its optimal hydrolase-producing conditions were as follows: 2.5% wheat bran as carbon source, 1% NaNO3 as nitrogen source, initial pH7. 0, culture medium volume 40mL/250mL, inoculating quantity 8% , culture temperature 30℃, revolutions 160r/min and culture time 84h. The enzyme activity reached 82 U/mL. Cu2+ can inhibit Absidia sp. R strain from producing the hydrolase, the influence of other metal ions was not remarkable on it.
3.Association of subclinical hypothyroidism with dyslipidemia and non-alcoholic fatty liver
Lingding XIE ; Yan-Ming GAO ; Gui-Zhi LU ; Qiu-Ming JIANG ; Xiao-Hui GUO ; Yan GAO
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
Objective To investigate the prevalence of subclinical hypothyroidism and its relation to serum total cholesterol (TC),triglyceride (TG) levels and non-alcoholic fatty liver.Methods Serum levels of TC,TG, TSH,TT_3 and TT_4 were determined in 1 602 subjects which were divided into groups by sex and age (20-39,40- 59 and≥60 years).Fatty liver was diagnosed by type B ultrasonography.Restults ( 1 ) Prevalence of subclinical hypothyroidism was 6.6% in this population.(2) The mean levels of serum TC and TG in subclinical hypothyroidism group and normal group showed no significant difference ( both P>0.05 ).(3) On the whole,the prevalences of subclinical hypothyroidism in hypercholesterolemia group and normal cholesterolemia group showed significant difference (P<0.01 ).(4) In the three age groups of male and few.ale examinees,the prevalences of subclinical hypothyroidism in hypercholesterulemla group and normal cholesterolemia group showed no significant difference ( all P>0.05 ).In the multiple regression analysis,TSH level was not related with raised TC level.(5) In the three age groups of male,there was no significant difference between the prevalences of subclinical hypothyroidism in hypertriglyceridemla group and normal triglyceridemia group ( all P>0.05 ).In female,expect for the elderly group (>60 years old ),the prevalence of subclinical hypothyroidism was higher in hypertriglyceridemia group than that in normal triglyceridemia.However,in the multiple regression analysis, increased TSH level was positively associated with increased serum TG level ( OR = 1.072,P=0.013 and OR = 1.102,P = 0.03 ).(6) The prevalence of subclinical hypothyroidism in non-alcobolic fatty liver group and normal group showed no significant difference ( P>0.05 ).In the multiple regression analysis,TSH level was not the independent risk factor of non-alcoholic fatty liver (P>0.05 ).Conclusion Subclinical hypothyroidism is not asseciated with serum TC level but positively associated with serum TG level.Subclinical hypothyroidism does not apparently increase the prevalence of non-alcoholic fatty liver.
4.Purification and properties of isoflavone-glucosidase.
Ming-Jie XIE ; Ming SONG ; Cui-Xia ZOU ; Chun-Hua XU ; Ming-Chun LU ; Feng-Xie JIN
Chinese Journal of Biotechnology 2006;22(4):635-638
A high activity isoflavone-glucosidase, which hydrolysis glycosides, was obtainde using liquid fermentation from Absidia sp. R strain. The isoflavone-glucosidase was purified 11 folds with yielding rate of 10.9% after ammonium sulfate precipitation and DEAE-Cellocuse (DE-52) ion exchange chromatography. SDS-PAGE results showed that the molecular weight is 53kD. And the optimum temperature, the optimum pH, Km and pI of the enzyme are 50 deegrees C, 5.0, 1.3 x 10(-2) mol/L and 3.2, respectively. The isoflavone-glucosidase is also rather stable under 60 degrees C and in pH range from 5.0 to 7.0. The enzyme can be activated by Co2+ and Ca2+, and be inhibited by Ag+ and Cu2+.
Absidia
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enzymology
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Glucosidases
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isolation & purification
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metabolism
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Hydrogen-Ion Concentration
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Isoflavones
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metabolism
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Temperature
5.The necessity and feasibility of establishing technical specifications for registry of Chinese medicine clinical study.
Peng-Fei LU ; Xing LIAO ; Yan-Ming XIE ; Zhi-Guo WANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(1):14-18
International clinical trials register is one of the global measures to realize transparency in clinical trials and also one of a powerful measure to improve the quality of clinical trials. Many scholars studying the quality of TCM clinical trials find that they are poor in quality and lack transparency. Furthermore, they find that TCM clinical trial registry has many problems. We must base on the successful experiences of WHO and international clinical trial registry to establish technical specifications for registry of traditional Chinese medicine clinical study of their own. Then, it can effectively improve the overall level of TCM clinical studies. We have suggested some concrete and feasible measures to establish technical specifications for registry of traditional Chinese medicine clinical study of their own based on the problems of TCM clinical trial registry.
Biomedical Research
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Humans
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Medicine, Chinese Traditional
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standards
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Registries
6.A study of HIV-related lipodystrophy syndrome in 55 HIV-infected Chinese adult patients
Lu ZHANG ; Ming LI ; Meimei TAO ; Zhifeng QIU ; Jing XIE ; Yang HAN ; Wei YU ; Taisheng LI
Chinese Journal of Internal Medicine 2009;48(2):118-121
Objective To study the prevalence, clinical characteristics and risk factors of HIV-related lipodystrophy syndrome (HIV-LD) in our cohort of HIV-1 infected Chinese adults. Methods In a cross-sectional study, 55 HIV-infected patients were recruited from the HIV clinic of Peking Union Medical College Hospital; most of them were undergoing the first-class highly active antiretroviral therapy (HAART) of today in China. Lipoatrophy or lipohypertrophy was defined if there was concordance between the report of fat change and clinical examination of the participants. Whole body dual-energy X-ray absorptiometry (DEXA) scanning was performed. Results Prevalence of clinical body fat redistribution in the present study was 47.3%. Comparing with non-LD patients, HIV-LD patients had elder age and longer exposure to HAART(P<0.05). HAART exposure and stavudine(d4T) usage were two independent risk factors for HIV-LD. Conclusions HIV-related fat redistribution does exist in Chinese HIV population. Peripheral lipoatrophy occurs commonly in HIV-infected adults but is not associated with increased trunk fat. HAART exposure and especially d4T usage are independent risk factors for HIV-LD.
7.Real-time three-dimensional echocardiographic assessment of left ventricular remodeling index and systolic function in patients with coronary artery disease
Ming CHEN ; Mingxing XIE ; Xinfang WANG ; Qing Lü ; Jing WANG ; Jing ZHANG ; Xiaofang LU
Chinese Journal of Ultrasonography 2008;17(9):737-740
Objective To explore left ventricular remodeling index(LVRI),systolic function and their correlation by real-time three-dimensional echocardiography(RT-3DE)in patients with coronary artery disease(CAD).Methods According to coronary artery angiography,one main vessel stenosis no less than 50% was defined as positive standard,RT-3DE data of 24 patients with left circumflex coronary artery or/and right coronary artery stenosis(LCA/RCA group),21 patients with left anterior descending coronary artery stenosis(LAD group),27 patients with double or triplex coronary arteries stenosis which must included LAD(vessels group)and 22 normal controls(NC group)were acquired.Left ventricular end-diastolic volume(EDV),left ventricular end-diastolic epicardial volume(EDVepi),left ventricular ejection fraction(EF)were automatically measured by RT-3DE analysis software;left ventricular mass[1.05×(EDVepi-EDV)],LVRI(left ventricular mass/EDV)were calculated.The inter-group differences of LVRI,EF and intra-group correlations between LVRI and EF were analyzed.Results LVRI and EF significantly decreased in order(P<0.05 or P<0.01);and their correlation coefficents were r=0.10(P>0.05),0.86(P<0.01),0.83(P<0.01)and 0.77(P<0.01)in NC group,LCA/RCA group,LAD group,vessels group,respectively.Conclusions LVRI measured by RT-3DE can assess left ventricular remodeling,and reflect left ventricular systolic function in different CAD,which can be as a new approach for evaluating left ventricular remodeling in clinic.
8.Recombination of RegIII-proinsulin-pBudCE4.1 plasmid and its therapeutic effect on STZ-induced type 1 diabetes mellitus.
Wenrui HOU ; Shengnan XIE ; Jingli LU ; Wei XI ; Xiang LUO ; Ming XIANG
Acta Pharmaceutica Sinica 2010;45(8):987-94
The aim of this study is to investigate the therapeutic effect of RegIII-proinsulin-pBudCE4.1 plasmid on streptozotocin (STZ)-induced type 1 diabetes mellitus and its underlying mechanisms. The model of type 1 diabetes mellitus was established by intraperitoneal injections of STZ (40 mg kg(-1)) to Balb/c mice for five consecutive days. Then, ten type 1 diabetic mice were intramuscularly injected with 100 microg RegIII-proinsulin-pBudCE4.1 plasmid for 4 weeks (one time/week) and the blood glucose levels were monitored every week; whereas another ten diabetic mice served as negative control group were injected with pBudCE4.1 vector at the same dose. Normal control and model control mice were treated with normal saline at identical volume under the same way. Western blotting, MTT assay, ELISA, HE staining and Tunel assay were applied to explore the underlying mechanisms. Results showed that RegIII-proinsulin-pBudCE4.1 plasmid ameliorated the hyperglycemia symptoms in diabetic mouse remarkably. It induced an immunological tolerance state in type 1 diabetic mice by inhibiting the proliferation of splenic lymphocytes and recovering Th1/Th2 balance evidenced by MTT and ELISA analysis. Furthermore, it elevated insulin concentration in the serum of type 1 diabetic mice and promoted the regeneration of beta cells supported by the results of HE staining and Tunel assay. In conclusion, RegIII-proinsulin-pBudCE4.1 plasmid possesses powerful anti-diabetic ability, which may be involved in the inducing of immunological tolerance and enhancing beta cells recovery.
9.Features of Clinical Register of Chinese Medicine and Pharmacy Based on ClinicalTrials.gov. (USA).
Peng-fei LU ; Xing LIAO ; Yan-ming XIE ; Zhi-guo WANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(11):1388-1392
In recent 10 years, clinical trials of Chinese medicine and pharmacy (cMP) at clinicalTrials.gov.(USA) are gradually increasing. In order to analyze features of CMP clinical register, ClinicalTrials.gov register database were comprehensively retrieved in this study. Included clinical trials were input one item after another using EXCEL. A final of 348 CMP clinical trials were included. Results showed that China occupied the first place in CMP clinical register, followed by USA. CMP clinical trials, sponsored mainly by colleges/universities and hospitals, mostly covered interventional studies on evaluating safety/effectiveness of CMP. The proportions of studies, sponsored by mainland China and companies, recruitment trials and multi-center clinical trials in interventional trials were increasing. The proportions of studies sponsored by Hong Kong and Taiwan, research completed trials, unclear research status, phase III clinical trials, and published research trials in interventional trials were decreasing. Published ratios of CMP clinical trials were quite low. There were more missing types and higher proportions in trial register information.
Biomedical Research
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China
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Clinical Trials as Topic
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Databases, Factual
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Humans
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Medicine, Chinese Traditional
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United States
10.Clinical value of combined detection of serological and biochemical test for Salmonellain
Xianmo WANG ; Zhangyuan YANG ; Liang MING ; Liangcai XIE ; Jinzhi LU ; Lin XIAO ; Yanlin LI
International Journal of Laboratory Medicine 2015;(11):1486-1487
Objective To explore the value of comprehensive biochemical and serological test in Salmonella clinical test .Methods A total of 95 cases of suspected salmonella infection with enteric fever were selected as subjects in this study from April 2013 to A‐pril 2014 ,and 48 cases randomly divided in research group ,47 cases in control group .Regular inspection was conducted in the con‐trol group ,comprehensive biochemical and serological test were conducted in the control group .Compared the results in the two groups .Results The salmonella detection rate in the research group was 93 .75% ,which was significant higher than 68 .09% in the control group (P< 0 .05) .Conclusion Combined detection of comprehensive biochemical and serology test could detect Salmonella effectively ,it is worthy of application and popularization in clinic .