2.Application of laparoscopy in assisted reproduction techniques
Ping LIN ; Ming WANG ; Maosheng GU
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To investigate the clinical significance of gamete intrafallopian transfer (GIFT) under laparoscope. Methods The gonadotropin was adopted to induce superovulation and the spermatic fluid was managed with the swim-up method. After oviums were dislodged by puncture under laparoscope, gametes were transplanted by intubating the fallopian tube. Results Among a total of 19 patients, 4 were pregnant, the pregnancy rate being 21.1% (4/19). Three women had a delivery at term and 1 had an abortion. No serious complications were seen. Of the 15 unfertilized patients, normal pregnancy was observed within 6 months after surgery in 5 patients, the circle pregnancy rate being 5.6% (5/89). Conclusions GIFT under laparoscope is a practical assisted reproductive technique (ART) that may achieve a relatively high pregnancy rate. Some other pelvic diseases resulting in infertility can be treated simultaneously, which contributes to natural fertilization in the future.
4.Ethics Thought of AIDS Epidemic Tendency and It's Harmfulness
Ming WANG ; Lin XU ; Hong XIAO ;
Chinese Medical Ethics 1994;0(05):-
This paper discussed AIDS epidemic sityation and it's tendency, AIDS harmful-ness to human beings health and social economic development. The dangerous factors of AIDS epidemic in china is pointed out. prsvent and treatment AIDS is urgency.
5.Novel mutation of low density lipoprotein receptor gene associated with familial hypercholesterolemia
Liwei CHEN ; Ming YANG ; Jie LIN ; Lvya WANG
Chinese Journal of Postgraduates of Medicine 2010;33(22):1-4
Objective To analyse the mutation of low density lipeprotein receptor (LDLR) gene associated with familial hypercholesterolemia (FH) and make a discussion on the relationship between genotype and phenotype. Methods The blood fat, electrocardiogram, heart and great vessels color Doppler were examined in propositus and family member. The promoter and all eighteen exons of LDLR gene were investigated by polymerase chain reaction (PCR),degenerate high performance liquid chromatogram (DHPLC) and DNA sequence analysis. The results were compared with the normal sequences in GenBank and FH database (www.ucl.uk/fh) to find the mutation. In addition,the apolipoprotein B100(ApoB100) gene for the known mutations(Q3500R) that cause familial defective ApoB100(FDB) was detected by directed screening.Results Two novel heterozygous c.1864 G→A (Asp622Asn) and c.1959 C→T(Val 653Val) mutations in the exon 13 of LDLR in promoter were detected. And Asp622Asn mutation segregated with the disease. No mutation Q3500R of ApeB100 was observed. Conclusions The heterozygous c.1864 G→A (Asp622Asn)mutation of LDLR gene is firstly determined in China. The heterozygous c.1864 G→A (Asp622Asn)mutation of LDLR gene is probably responsible for FH. Perhaps it is a particular pathogenesis for Chinese people. PCB-DHPLC could be used for detecting the mutation.
6.CT Study of the Internal Occipital Protuberance
Lin LUO ; Bolang YU ; Zezhong WANG ; Ming ZHANG
Journal of Practical Radiology 1992;0(11):-
Objective To study the development and shape of the occipital protuberance and its clinical significance with normal cranial CT scans.Methods 3000 cases of normal cranial CT images were selected.There were 1500 males and 1500 females,which were divided into nine age groups.The internal occipital protuberances were observed and measured on bone-window.Results The internal occipital protuberance was seen earlist at four-month-old-infant,and growth with age.The shape of the internal occipital protuberance was classified into four types:single-peak-type,plane-protusion-type,tropezoid-type and irregular-type.The single-peak-type was seen most commonly.Big semilunar depression of the internal occipital protuberance could be seen in patient with magna citern arachnoid cyst.Conclusion The height and shape of normal internal occipital protuberance have many diversities.Big semilunar depression of the internal protuberance is a important sign in diagnosis of magna cistern arachnoid cyst.
7.Content determination of dencichine in Panax Notoginseng by a reversed phase ion-pair chromatography.
Lin LI ; Cheng-xiao WANG ; Yuan QU ; Xiu-ming CUI
China Journal of Chinese Materia Medica 2015;40(20):4026-4030
To build a reversed phase ion-pair chromatography to determination content of Dencichine from Panax notoginseng. Using Tetrabutyl ammonium hydroxide ions by the combination of reagent and HPLC method without derivatization to test the content of dencichine directly. The optimum conditions of supersonic extraction were solid-to-liquid ratio 1: 20, Continuous ultrasonic extraction: twice, each time 15 minutes; 3,500 r · min⁻¹, then centrifuging 15 minutes. Dencichine in different age, place, part and the different Processing mode were examined. The method is simple with sound separation degree and stability, which can facilitate the determination of dencichine content directly and provide the basis in quality standard of raw material.
Amino Acids, Diamino
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analysis
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Chromatography, Reverse-Phase
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methods
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Drugs, Chinese Herbal
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analysis
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Panax notoginseng
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chemistry
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Plant Roots
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chemistry
8.Related factors of death in patients with gas burst induced burn.
Lin-hong LIU ; Wen-jie REN ; Ming-jun WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(10):611-613
Adult
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Burns
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etiology
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mortality
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China
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epidemiology
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Coal Mining
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Explosions
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Female
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Gases
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Humans
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Male
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Middle Aged
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Retrospective Studies
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Young Adult
9.Clinical analysis of 221 cases of inhalation injury induced by gas explosion.
Lin-hong LIU ; Wen-jie REN ; Ming-jun WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(8):494-495
Adolescent
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Adult
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Burns, Inhalation
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etiology
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therapy
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Explosions
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Female
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Gases
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Humans
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Male
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Retrospective Studies
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Young Adult
10.Pleomorphic adenoma with extensive lipometaplasia in the parotid gland: report of two cases.
Mei KONG ; Qi-lin SHI ; Zhao-ming WANG
Chinese Journal of Pathology 2007;36(5):350-352
Adenoma, Pleomorphic
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metabolism
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pathology
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surgery
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Adipose Tissue
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pathology
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Female
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Follow-Up Studies
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Humans
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Immunohistochemistry
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Male
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Membrane Proteins
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metabolism
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Metaplasia
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pathology
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Middle Aged
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Parotid Gland
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metabolism
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pathology
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surgery
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Parotid Neoplasms
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metabolism
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pathology
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surgery
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S100 Proteins
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metabolism