2.Application of laparoscopy in assisted reproduction techniques
Ping LIN ; Ming WANG ; Maosheng GU
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To investigate the clinical significance of gamete intrafallopian transfer (GIFT) under laparoscope. Methods The gonadotropin was adopted to induce superovulation and the spermatic fluid was managed with the swim-up method. After oviums were dislodged by puncture under laparoscope, gametes were transplanted by intubating the fallopian tube. Results Among a total of 19 patients, 4 were pregnant, the pregnancy rate being 21.1% (4/19). Three women had a delivery at term and 1 had an abortion. No serious complications were seen. Of the 15 unfertilized patients, normal pregnancy was observed within 6 months after surgery in 5 patients, the circle pregnancy rate being 5.6% (5/89). Conclusions GIFT under laparoscope is a practical assisted reproductive technique (ART) that may achieve a relatively high pregnancy rate. Some other pelvic diseases resulting in infertility can be treated simultaneously, which contributes to natural fertilization in the future.
4.Ethics Thought of AIDS Epidemic Tendency and It's Harmfulness
Ming WANG ; Lin XU ; Hong XIAO ;
Chinese Medical Ethics 1994;0(05):-
This paper discussed AIDS epidemic sityation and it's tendency, AIDS harmful-ness to human beings health and social economic development. The dangerous factors of AIDS epidemic in china is pointed out. prsvent and treatment AIDS is urgency.
7.Proventive effects of astaxanthin on metabolic cataract and its mechanism in type 1 diabetic rats
Ming, YANG ; Zhiju, WANG ; Tingting, DENG ; Lin, PAN
Chinese Journal of Experimental Ophthalmology 2017;35(3):217-224
Background The pathogenesis mechanism of diabetic cataract has not been fully elucidated.Researches showed that multiple biological pathways participate in the pathogenesis of diabetic cataract,including oxidative stress.Astaxanthin can inhibit oxidative stress-mediated injury and lipid peroxidation.However,whether astaxanthin has the preventive effects on diabetic cataract is unclear.Objective This study was to investigate the preventive effects of astaxanthin on metabolic cataract in type 1 diabetic rats.Methods Thirty-eight 6-week-old SPF male SD rats were used in this study,and 1% streptozocin was intraperitoneally injected to establish type 1 diabetic models in 30 rats,and 24 successful models were assigned to diabetic model group,low-dose astaxanthin group and high-dose astaxanthin group.Equal volume of normal saline solution was injected in the same way in 8 rats as the normal control group.Mixture foods containing 50 mg/(kg · day) or 100 mg/(kg · day) astaxanthin with olive oil and fodder were used continuously for 3 months in the rats of low-dose astaxanthin group and high-dose astaxanthin group,respectively,and mixture food of olive oil with fodder was used in the diabetic model group.Only fodder was used in the same way in the rats of the normal control group.The opacification of lens was examined by slit lamp section radiography system and graded on a scale of 1-5.The specimen of lens were prepared for the hematoxylin & eosin stain.The expression and lation of advanced glycosylation end products (AGEs) in the lens was examined using immunochemistry.The contents of oxidative stress-related indicators in the lens,such as AGEs,malonydialdehyde (MDA),catalase (CAT),superoxide dismutase (SOD) and mass fraction of glutathione (GSH),were assayed by ELISA.The experimental process complied with the national standard (Laboratory Animal Requirements of Environment and Housing Facilities [GB14925-2001]).Results The blood glucose levels of the rats were significantly higher in the diabetic model group,low-dose astaxanthin group and high-dose astaxanthin group than those in the normal control group at 2,4,6,8,10 and 12 weeks after modeling (all at P<0.05),while the blood glucose levels of rats were not evidently different between low-dose astaxanthin group and high-dose astaxanthin group at various time points(all at P>0.05).The rat lenses were transparent in the normal control group with scale of grade 1,and serious lens opacification was seen in the rats of the diabatic model group,with the scale of grade 5,while the rat lenses in the low-dose astaxanthin group and high-dose astaxanthin group were in grade 3-4.The contents of AGEs in the lenses were (7.23 ±0.50) μg/ml and (7.01 ±0.37) μg/ml,and M DA contents were (1.43 ± 0.22) mmol/L and (1.35±0.16)mmol/L in the low-dose astaxanthin group and high-dose astaxanthin group respectively,which were significantly lower than (7.61± 0.45) μg/ml and (1.62 ±0.42) mmol/L in the normal control group (all at P<0.05).GSH contents in rat lenes were (272.70±12.53) ng/L and (283.52±16.17) ng/L,and SOD coneents were (55.45± 6.47) μmol/(min · L) and (56.73±5.12) μmol/(min · L),and CAT concents were (2.91 ±0.41) μmol/(min · L)and (3.02±0.13)μmol/ (min · L) in the low-dose astaxanthin group and high-dose astaxanthin group respectively,which were significantly higher than (241.52 ± 15.13) ng/L,(51.67 ± 5.45) μmol/(min · L) and (2.72 ± 0.27)μmol/(min · L) in the normal control group (all at P<0.05).The GSH concent and SOD concent in rat lens were lower in the low-dose astaxanthin group than that in the high-dose astaxanthin group (both at P<0.05).Conclusions Astaxanthin can postpone the pathogenesis and development of diabetic cataract in type 1 diabetic rats by antioxydative stress.
8.Advance in Functional Exercise on Low Back Pain (review)
Shaoqing CHEN ; Jianping LIN ; Ming LI ; Shizhong WANG
Chinese Journal of Rehabilitation Theory and Practice 2013;19(10):936-938
The decline in lumbar function is the main reason of lumbago repeated attacks. Although the treatment of low back pain included surgery, drugs and other non-medical treatments, foreign system evaluation has confirmed that functional exercise was better than the conventional treatment for patients with chronic low back pain. This article introduced the mechanism and methods of functional exercise.
9.Pleomorphic adenoma with extensive lipometaplasia in the parotid gland: report of two cases.
Mei KONG ; Qi-lin SHI ; Zhao-ming WANG
Chinese Journal of Pathology 2007;36(5):350-352
Adenoma, Pleomorphic
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metabolism
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pathology
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surgery
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Adipose Tissue
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pathology
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Female
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Follow-Up Studies
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Humans
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Immunohistochemistry
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Male
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Membrane Proteins
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metabolism
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Metaplasia
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pathology
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Middle Aged
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Parotid Gland
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metabolism
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pathology
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surgery
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Parotid Neoplasms
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metabolism
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pathology
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surgery
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S100 Proteins
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metabolism
10.Related factors of death in patients with gas burst induced burn.
Lin-hong LIU ; Wen-jie REN ; Ming-jun WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(10):611-613
Adult
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Burns
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etiology
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mortality
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China
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epidemiology
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Coal Mining
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Explosions
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Female
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Gases
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Humans
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Male
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Middle Aged
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Retrospective Studies
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Young Adult