1.Preparation Techniques of Ginkgolides Drop Pills by Orthogonal Design
Shengrong SHAO ; Jun SUN ; Ming WU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(07):-
Objective To optimize the preparation condition of Ginkgolides drop pill. Methods The technique of preparation drop pill were optimized by orthogonal test. Results The optimized technique to prepare Ginkgolides drop pills:PEG4000∶PEG6000=1∶1,the temperature for the aqueous mixture was 85 ℃,the temperature for cool aqueous was 5 ℃,active ingredients∶excipient was 1∶3. Conclusion good quality of Ginkgolides drop pill can produced.
2.High Efficiency Expression of the Gene From Herpes Simplex Virus Type 1(HSV-1)Glycoprotein D
Jun-Bo YI ; Ming-Jun LEI ; Shao-Juan CHEN ; DE-XIN ; Ling-Yun LI ;
China Biotechnology 2006;0(05):-
Objective:To obtain the high expression of Herpes Simplex Virus Type 1(HSV-1)Glycoprotein D gene. Methods:The Herpes Simplex Virus Type 1(HSV-1)Glycoprotein D(gD1) gene fragment containing dominant antigen epitopes confirmed by computer analysis was cloned by PCR technical and inserted into plasmid vector pTrxA. Then the recombinant plasmid was transformed into Rosetta. The expressed product was analyze by SDS-PAGE. Results:930 bp gene fragment was amplified by PCR as anticipated. Nucleotide sequencing showed a 100 % homology with that of the published sequence in GenBank. The molecular weight of the expressed protein was about 48kDa, Western blotting indicated that the antigenicity of the protein was good. Conclusion:The plasmid pTrxA-gd1 was constructed and a high efficiency expression of the gd1 gene from Herpes Simplex Virus Type 1(HSV-1)strain was made. The expressed product shows a good antigenicity.
3.Chromosomal aberration in human keloid analyzed by comparative genomic hybridization.
Gang ZHANG ; Shao-jun LUO ; Shao-ming TANG ; Jie LIANG
Chinese Journal of Plastic Surgery 2005;21(1):29-31
OBJECTIVETo identify the genetic alteration in human keloid.
METHODSComparative genomic hybridization was applied in 6 cases of keloid to investigate the genomic imbalance (the gain or loss of genetic material).
RESULTSThe study showed that the loss of chromosome DNA copies included chromosome, 1,7,9,13,16,17,18,19,20,22. Among them, the frequently detected chromosome loss was chromosome 1 p(66.7%), 16 (83.3%), 20 (83.3%) and 22 (83.3%). The minimum overlapping regions were 1 pter-32.2,16p13.2p11.l,20q11.1-q13.2 and 16p13.2-p11.1. Frequent gain of DNA copy numbers was not found in the special regions.
CONCLUSIONSThe mapping of DNA copy variation frequency in keloid showed that there may be inhibitory genes in chromosomes 1p,16,20,22. The loss of these genes may be involved in the development and progress of keloid.
Adult ; Chromosome Aberrations ; Chromosomes, Human, Pair 1 ; genetics ; Comparative Genomic Hybridization ; DNA Probes ; Female ; Humans ; Keloid ; genetics ; Male ; Middle Aged ; Young Adult
4.Comparative genomic hybridization analysis of nonsyndromic cleft lip with palate.
Wen-Guang ZHANG ; Shao-Jun LUO ; Shao-Fang ZHU ; Shao-Ming TANG
Chinese Journal of Plastic Surgery 2008;24(2):123-125
OBJECTIVETo identify the genetic alterations in nonsyndromic cleft lip and palate (NSCLP).
METHODSComparative genomic hybridization was applied to investigate the genomic imbalance (the gain or loss of genetic material) in 7 cases of NSCLP.
RESULTSIt showed that the loss of chromosome DNA copies happened in chromosome 6, 7, 10, 13, 14, 16, 20, 22 and the gain of chromosome DNA copies happened in chromosome 5, 15, 18, 19. Conclusions 13q had a high frequency (71.4%) of chromosome loss.
CONCLUSIONSAbnormal chromosome DNA copies happen in all the patients with NSCLP. Most of the patients have chromosome DNA copies loss. It suggests that loss of inhibitory gene may be related to the NSCLP. The related inhibitory gene may locate in 13q.
Adolescent ; Adult ; Child ; Child, Preschool ; China ; ethnology ; Cleft Lip ; genetics ; Cleft Palate ; genetics ; Comparative Genomic Hybridization ; DNA ; Genetic Variation ; Genotype ; Humans ; Infant ; Mutation ; Phenotype ; Young Adult
5.Synthesis and characterization of 9-O-alkyl substituted palmatine derivatives.
Zi-Ming LV ; Shao-Hua ZHAO ; Jun-Qing LIANG ; Peng-Fei TU
China Journal of Chinese Materia Medica 2014;39(4):699-703
For the establishment of chemical library of protoberberines provided for the bio-activity screening, the target compounds were synthesized by thermal degradation and nucleophilic substitution reactions with the bio-active alkaloid, palmatine (1), as the raw material, and their structures were identified and conformed by 1H-NMR and MS spectra. Among them, 13 compounds were new.
Berberine Alkaloids
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chemical synthesis
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chemistry
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Drugs, Chinese Herbal
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chemical synthesis
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chemistry
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Molecular Structure
6.Effect of tensile stress on human heel skin fibroblast proliferation in vitro.
Jun-hua LI ; Zhong-ming HUANG ; Shao-hua DU ; Li-gang HUANG
China Journal of Orthopaedics and Traumatology 2014;27(10):838-842
OBJECTIVETo observe the effect of tensile stress on human heel skin fibroblast proliferation in vitro, providing a theoretical basis for preventing the wound edge skin necrosis and nonunion after calcaneal fracture surgery.
METHODSFibroblast cells were taken from lateral heel skin of a 40 year-old-man, then cultured and subcultured in vitro. After that, they were divided into three groups: 0 hours group, 6 hours group and 24 hours group and were tested by tensile stress testing. The levels of TGF-β1 and IL-6 in nutrient fluid were measured. Transmission electron microscope and light microscope was applied for observe mitochondria and nucleus.
RESULTSUnder 10% of the tensile stress, mitochondria decreased, the levels of TGF-β1 and IL-6 in nutrient fluid were decreased and cell proliferation was inhibited gradually with time increasing.
CONCLUSIONThe human lateral heel skin in a long-time tensile stress state is an important cause of wound edge skin necrosis and nonunion after calcaneus fracture surgery.
Adult ; Cell Proliferation ; Cells, Cultured ; Fibroblasts ; chemistry ; cytology ; Heel ; physiology ; Humans ; In Vitro Techniques ; Interleukin-6 ; metabolism ; Male ; Skin ; chemistry ; cytology ; metabolism ; Tensile Strength ; Transforming Growth Factor beta1 ; metabolism
7.Multi-dimensional structure quality control over Salvia miltiorrhiza injection based on component structure theory.
Shao-Ying HU ; Liang FENG ; Ming-Hua ZHANG ; Jun-Fei GU ; Xiao-Bin JIA
China Journal of Chinese Materia Medica 2013;38(24):4375-4378
As the preparation process from Salvia miltiorrhiz herbs to S. miltiorrhiz injection involves complicated technology and has relatively more factors impacting quality safety, the overall quality control is required for its effectiveness and safety. On the basis of the component structure theory, and according to the material basis of S. miltiorrhiz injection, we discussed the multi-dimensional structure and process dynamic quality control technology system of the preparation, in order to achieve the quality control over the material basis with safety and effectiveness of S. miltiorrhiz injection, and provide new ideas and methods for production quality standardization of S. miltiorrhis injection.
Drug Compounding
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Drugs, Chinese Herbal
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administration & dosage
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adverse effects
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chemistry
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Injections
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Quality Control
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Safety
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Salvia miltiorrhiza
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chemistry
9.Significance of examine of??T and NKT cells in non-small-cell lung cancer
Shao-Yun ZHANG ; Jun-Ping ZHANG ; Su-Ying ZHANG ; ZHAO-PEI ; Ming-Ying SHI ;
Cancer Research and Clinic 2001;0(04):-
Objective To open out new method,we probe into immune function of??ST and NKT cells in NSCLC.Methods The peripheral blood cells were stained with antibodies labeled with fluorescence in NSCLC,??T,NKT and their set group cells were counted with flow cytometry.Results The absolute counts of??T,CD~+_(56)??T,CD~-_(56)??T,NKT and??T~+ NKT cells in NSCLC was significantly lower than that of normal controls.The relative counts of NKT,??T~+ NKT,??T~-NKT and CD~+_56??T cells in NSCLC was not sig- nificantly lower than that of normal control.The absolute counts of??T cells in NSCLC positively correlated to the number of NKT cells,(r=0.426,P=0.009).Conclusion The absolute counts of??T,NKT and their set ground cells in NSCLC was significantly lower than that of normal controls.In NSCLC the relation of the??T and NKT had positive correlation.
10.DSA diagnosis and embolization therapy of gastrointestinal hemorrhage
Ru-Ming ZHOU ; Shui-Bo QIU ; Min-Hua LIU ; Hui-Jun YANG ; Shao-Yu ZHUANG ;
Chinese Journal of Radiology 1999;0(10):-
Objective To evaluate the effect of digital subtraction angiography and transcatheter embolization for gastrointestinal hemorrhage.Methods Twenty patients with gastrointestinal hemorrhage received celiac arteries,superior mesenteric arteries and inferior mesenteric arteries angiography. Superselective angiography were performed when the arteries were suspicious by clinic or angiogrraphy.Ten patients with definite diagnosis and manifestation of hemorrhagic arteries by angiography were embolized after superseleetive catheterization with gelfoam particles,gelfoam particles and coils,polyvinyl alcohol particles. Results The positive signs were observed in 13 cases.The DSA features including contrast medium accumulation in the gastrointestinal tract outside vascular,aneurysm,tumorous vascularization and staining, artery affect and local vasospasm.The bleedings were stopped immediately in 8 patients.No rebleeding and intestinal ischaemia or necrosis were observed in 30 days.One patient died in the second day after embolization from multiple organ failure.Rebleeding occurred 3 days after embolization in another patient, and was recovered after surgical operation.Conclusion DSA is more effective for the diagnosis of gastrointestinal vascular malformation and tumors complicating acute bleeding.Transcatheter embolization is effective and safe to control the hemorrhage.