The mature seeds and excised embryos of Dalbergia odorifera were used as materials to study the effect of moisture content on their survival, as well as the effect of rapid freezing and vitrification freezing method on seeds and in vitro embryos cryopreservation. The results showed that the germination rate and vigor decreased from 82.67%, 85% to 18.35%, 25% respectively, when the seed moisture content decreased from 15.04% to 8.14%; and the germination rate decreased from 82.67% to 37.50%, 25.37% respectively by vitrification freezing method and rapid freezing method, when the seed moisture content decreased from 15.04% to 9.37%. Among all the moisture content gradient, 12.35% moisture reached the maximal germination rate, which were 63.58% and 50.45% respectively by vitrification freezing and rapid freezing; and when the embryo moisture content was 26.32%, the germination rate decreased from 95.67% to 58.31% and 33.82% respectively by vitrification freezing and rapid freezing. And when the moisture content was in the range of 14.17% -21.34%, the germination rate was a bit of decrease. The experiment results showed that the optimum conditions of seed cryopreservation were: moisture content 12.35%, vitrification freezing; and the optimum conditions of in vitro embryo cryopreservation were: moisture 15.04%, vitrification freezing. In conclusion, the effects of moisture content on germination rate after cryopreservation in D. odorifera seeds and embryo were significant, and vitrification freezing method is much better than rapid freezing method.
Cryopreservation ; methods ; Dalbergia ; chemistry ; growth & development ; Germination ; Seeds ; chemistry ; growth & development ; Water ; analysis

Objective To establish a sensitive,specific,simple and high-throughput method for detection of the epidermal growth factor receptor (EGFR) mutation in the plasma samples of patients with non-small cell lung cancer (NSCLS) by the use of mutant-enriched liquid chip (MEL) assay.Methods The specific probes for the EGFR exon19 E746-750 deletion,exon 21 L858R mutation and wild-type sequence were designed and coupled to the microspheres coding with different fluorescent dye.The probe coupling efficiency was verified by crossing hybridization test with biotin-labeled reverse sequence.A blood-based MEL approach which integrates a sensitive mutant-enriched PCR and quantitative high throughput liquid chip assay for assessment of EGFR mutations was developed.The sensitivity and specificity of MEL was further evaluated using the mixture with different copy numbers of mutant and wild-type plasmids as template.The mutations of exon 19 and 21 of EGFR gene in plasma samples from 201 patients with stage ⅢB or Ⅳ NSCLC who enrolled in the First Affiliated Hospital of Guangzhou Medical College from September 2008 to April 2010 were analyzed by both the MEL and the mutant-enriched PCR assay.The result comparison was made between direct sequencing and MEL in 50 cases whose EGFR gene type had been tested by MEL and mutantenriched PCR.The correlation of EGFR gene mutation and the response to the Geftinib treatment was analyzed in 16 patients with lung adenocarcinoma as well.Results The probes were successfully coupled to the microspheres encoding with different fluorescent dye,and could be specifically recognized by the corresponding target sequence.The MEL was capable of detecting as few as 10 copies of EGFR mutants (sensitivity was 0.1％).Among the enrolled 201 cases of advanced NSCLC,the detection rate of the EGFR exon19 E746-750 del and exon 21 L858R was 55.7％ (112/201) by MEL assay.Conpared with mutantenriched PCR[58.2％ (117/201)],the coincidence rate was 97.5％ (196/201).There was no statistically significant difference between the results of mutant-enriched PCR and MEL (x2 =3.20,P ＞ 0.05).The mutations detection rate was 22.0％ (11/50) by directing sequencing,which was significantly lower than by MEL[50.0％ (25/50),x2 =12.07,P ＜ 0.05].Among the 16 patients treated with Gefitinib,9 cases who had EGFR mutation showed a higher response rate(P =0.041)and prolonged progression-free survival (x2 =6.76,P =0.009) after the treatment compared to those 7 who without EGFR mutation.Conclusions A new method of MEL with accuracy,specificity,fast and high-throughput is established for the detection of EGFR 19 E746-750 deletion and exon 21 L858R mutations in plasma from advanced NSCLC patients.It has the ability to provide the most direct and valuable guidance for clinicians to make decision on EGFR tyrosine kinase inhibitors therapy in the advanced NSCLC paticnts.

Anti-Infective Agents ; therapeutic use ; Burkholderia pseudomallei ; drug effects ; isolation & purification ; Humans ; Male ; Melioidosis ; drug therapy ; microbiology ; pathology ; Middle Aged ; Skin Diseases, Bacterial ; drug therapy ; microbiology ; pathology ; Trimethoprim, Sulfamethoxazole Drug Combination ; therapeutic use

Adult ; Chemical and Drug Induced Liver Injury ; Chronic Disease ; Critical Illness ; Dimethylformamide ; poisoning ; Humans ; Male ; Middle Aged ; Occupational Diseases ; chemically induced ; Occupational Exposure

The self-designed experiments of extract preparations is practiced in pharmacy teach-ing. Students work in groups, first consulting literature material, selecting the prescription and devis-ing a plan. Then, if the plan has carried on the feasible proof, students begin to purchase raw materi-als, complete the extraction and separation of effective components, the preparation of molding and the analysis of experimental results and finally form their self-evaluation. This experiment can strengthen students' experiment skill and promote their comprehensive application ability of all the subjects in the pharmacy field. At the same time it can also play an important role in the improvement of the students' social practice ability and the cultivation of students' creative thinking.

BACKGROUND:In total hip arthroplasty, the accurate placement of the acetabulum is needed to guarantee the survival rate of the prosthesis and improve the prognosis. In order to ensure the accurate placement of the acetabulum, the accurate measurement of the abduction angle and the anteversion angle of the acetabulum is needed. OBJECTIVE:To investigate the application value of C-arm X-ray displacement measurement of acetabular anteversion angle and abduction angle in total hip arthroplasty. METHODS:Total y 63 cases undergoing total hip arthroplasty were divided into two groups according to their wil . Patients in the control group (n=30) were implanted with traditional acetabular prosthesis locator. Patients in the observation group (n=33) were implanted with acetabular prosthesis after C-arm X-ray displacement measurement. Acetabular anteversion angle, abduction angle and pelvic inclination were measured before, during and after arthroplasty. Acetabular anteversion angle and abduction angle were measured in both groups after arthroplasty. Pain score and hip function Harris score were recorded in both groups at different time points. RESULTS AND CONCLUSION:(1) No significant difference in acetabular anteversion angle, abduction angle and pelvic inclination was detected before, during and after arthroplasty (al P>0.05). (2) No significant difference in abduction angle was determined between the two groups after arthroplasty (P>0.05), but acetabular anteversion angle was significantly smal er in the observation group than in the control group (P<0.05). (3) At 7 days after arthroplasty, Visual Analog Scale scores were less in the observation group than in the control group (P<0.05). (4) Compared with that before arthroplasty, Harris score was significantly higher after arthroplasty in both groups (P<0.05). Harris score was higher in the observation group than in the control group at 3 and 12 months after arthroplasty (P<0.05). (5) Results indicated that during total hip arthroplasty, C-arm X-ray machine can measure acetabular anteversion angle and abduction angle, and effectively correct pelvic inclination, internal and external rotation, abduction and adduction, improve the accuracy of acetabular cup placement and the quality of replacement surgery, and improve the prognosis of the patients.

Child ; Humans ; Plasma Exchange ; Thrombotic Microangiopathies ; therapy

Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Scalp ; Sleep Initiation and Maintenance Disorders ; therapy ; Treatment Outcome ; Young Adult

Antibodies ; analysis ; Biopsy ; Child ; Child, Preschool ; Disease Progression ; Drug Resistance ; Female ; Fluorescent Antibody Technique ; Humans ; Immunoglobulin A ; analysis ; Kidney Glomerulus ; immunology ; pathology ; Male ; Nephrosis ; drug therapy ; pathology ; Prognosis ; Steroids ; pharmacology ; therapeutic use

Calcium-Binding Proteins ; metabolism ; Diagnosis, Differential ; Female ; Fibroma ; metabolism ; pathology ; surgery ; Humans ; Inclusion Bodies ; pathology ; Infant ; Microfilament Proteins ; metabolism ; Skin Neoplasms ; metabolism ; pathology ; surgery ; Soft Tissue Neoplasms ; pathology ; Tendons ; pathology ; Toes ; Vimentin ; metabolism