OBJECTIVETo explore the effect of Tanshinone II A on severe acute pancreatitis (SAP) lung injury (ALI) rats and its possible mechanism.

METHODSSD rats were injected with sodium taurocholate to induce SAP group, and then intervened with sodium tanshinone II A sulfonate ( STS group). Simultaneously a sham-operation group (SO group) was set up. There were 24 rats in each group. The survival state and wet-to-dry weight ratio of lung tissues were observed. Activities of myeloperoxidase (MPO) in lung were determined by MPO reagent kit. Pathologic changes of lung tissues were determined by Hofbuaer method. Expression levels of three cytokines, TNF-alpha, IL-1beta, and intercellular cell adhesion molecule-1 (ICAM-1) were detected by ELISA.

RESULTSThe survival state of rats in the SAP group was deteriorated. The wet-to-dry weight ratio, MPO activities, pathologic changes in lung tissues, and expression levels of TNF-alpha, IL-1beta, and ICAM-1 increased significantly more in the SAP group than in the SO group (P < 0.05). Compared with those in the SAP group, the survival state of rats in the STS group was improved; the wet-to-dry weight ratio, MPO activities, pathologic changes in lung tissues, and expression levels of TNF-alpha, IL-1beta, and ICAM-1 obviously decreased in the STS group (P < 0.05).

CONCLUSIONTanshinone II A had remarkable effect on SPA LI rats, which might be associated with changing cytokines levels and attenuating infiltration of lung inflammatory cells.

Acute Lung Injury ; drug therapy ; Animals ; Cytokines ; metabolism ; Diterpenes, Abietane ; pharmacology ; therapeutic use ; Intercellular Adhesion Molecule-1 ; Interleukin-1beta ; Lung ; Pancreatitis ; drug therapy ; Peroxidase ; Rats ; Rats, Sprague-Dawley ; Taurocholic Acid ; Tumor Necrosis Factor-alpha

Objective: To study the inhibition and the mechanism of Hygrophorus lucorum polysaccharide (HLP) on H22 transplanted tumor in mice. Methods: The H22 transplanted models of mice were established. Sixty mice were divided into six groups: control, model, cytoxan (CTX, 200 mg/kg) positive control, low-, mid-, and high-dose (50, 100, and 200 mg/kg) HLP groups. On the day 2 after being inoculated with H22 tumor cells, mice were ig given HLP once daily for consecutive 10 d. And then the body weight change, tumor growth inhibitory rate, and spleen and thymus indexes were calculated; the serum levels of TNF-α, IL-2, VEGF, and albumin (Alb) were determined. The activities of SOD, GSH-Px, CAT, and the contents of GSH and MDA in liver homogenates, as well as the number of WBC were detected. Results: The tumor growth inhibitory rate of HLP was over 30%. The treatment with HLP significantly increased the body weight, spleen index, and the number of WBC, elevated the serum levels of IL-2, reduced the VEGF, promoted the hepatic SOD, GSH-Px, CAT activities, and GSH content, and decreased the MDA in liver homogenates. Conclusion: HLP has an antitumor effect on H22 transplanted tumor in mice, and the possible mechanisms may be due to its antioxidant activity, regulation of immunofunction, and anti-angiogenetic action.

Aged ; Diagnosis, Differential ; Gastrectomy ; methods ; Gastrointestinal Stromal Tumors ; metabolism ; pathology ; Humans ; Lipoma ; pathology ; Liposarcoma ; metabolism ; pathology ; surgery ; Male ; S100 Proteins ; metabolism ; Stomach Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective To investigate the effects and mechanisms of selective angiotensinⅡtype 1 receptor antagonist ZD7155 on the inhibition of pancreatic cancer in vitro.Methods MTT assays were used to determine the inhibition of pancreatic cancer cell line PaTu8988s by ZD7155 in different concen- trations and at different time.PaTu8988s cell cycle and cell apoptosis were detected by flow cytometry. Transmission electron microscope was used to investigate the apoptosis of PaTu8988s before and after the incubation with ZD7155 under different concentrations.PaTu8988s cell morphology was observed be- fore and after the incubation with ZD7155.Results MTT showed that the increase of inhibition of pan- creatic cancer cell by ZD7155 was in agreement with the increase of the concentrations of ZD7155 and the time of the incubation with ZD7155.The inhibition rates of PaTu8988s cells were 9%,18%,30%, 51%,60% and 78% by ZD7155 with the concentrations of 5?10~(-11),5?10~(-10),5?10~(-9),5?10~(-8),5?10~(-7) and 5?10~(-6) mol/L,respectively.The inhibition rates of PaTu8988s cells were 15%,25%, 36%,51%,67% and 85% by ZD7155 with the same concentration(5.0?10~(-8) mol/L)at 12,24,36, 48,60 and 72 hours,respectively.ZD7155 could also inhibit PaTu8988s cell cycle significantly and was dose-dependent.Cell electron microscopy showed that there were chromatin margination and apoptotic body in the cell nucleus when the cells were incubated with ZD7155,and these changes were increase with the concentrations of ZD7155.The morphology of PaTu8988s cell didn't have any change after in- cubation with ZD7155.Conclusions ZD7155 can inhibit the growth of pancreatic cancer cells in vitro by suppressing the S-phase of cell cycle and induce cell apoptosis without visible cell toxic effects.

Objective:To investigate the regulation of acupuncture on γ-interferon (INF-γ) and tumor necrosis factor (TNF) of lung cancer-operative cases. Methods: to determine the INF-γ and TNF contents in the blood serum of lung cancer patients by double antibody sandwich immuno-enzymatic method (ELISA); to measure the INF-γ and TNF contents of 30 lung cancer patients in the acupuncture anesthesia group and 30 lung cancer patients in general anesthesia group before the operation and at the 8th days, the 12th day after the operation respectively and make comparison between the two groups. Results:The pre-operation INF-γ contents of the two groups showed no significant difference (P＞0.05); the post-operation INF-γ contents of the two groups showed significant difference at 8th day and 12th day after the operation (P＜0.05); the acupuncture anesthesia group was superior to the general anesthesia group; the self-comparison of the anesthesia group showed significant difference at the 12th day and 8th day after the operation (P＜0.05); the pre-operation TNF contents of the two groups showed no significant difference (P＞0.05) and the post-operation TNF contents of the two groups showed significant difference at the 8th day and 12th day after the operation (P＜0.05). Conclusion:Acupuncture can increase the serum INF-γ and TNF contents of lung cancer patients and therefore regulate the immunity of the patients.

Studying the industry standard of electroacupuncture therapy device (YY 0780-2010), collaborating with the clinical practice of acupuncture and moxibustion, in view of academy and safety, the comments and suggestions are proposed on the content of the standard. The standard describes manipulation norms, terms and definitions, dianjizhen and the output energy of single pulse, etc. It is expected that these comments and suggestions can be taken in consideration in the revision of industry standard or the development of national standard so as to improve the scientific level and feasibility of the technique standard of electroacupuncture therapy device.
Cooperative Behavior ; Electroacupuncture ; instrumentation ; methods ; Equipment and Supplies ; standards ; Humans

Objective:To investigate the inhibitory effect of selective angiotensinⅡtype 1 receptor antagonist ZD7155 on pancreatic cancer xenografts of nude mice.Methods:Sixty nude mice were given subcutaneous injections of PaTu8988s cells to establish the pancreatic cancer xenograft models;then the animal models were evenly randomized into 3 groups:low-dose (10 mg?kg~(-1)?d~(-1))ZD7155,high-dose(20 mg?kg~(-1)?d~(-1))ZD7155 and normal saline groups.Ten mice in each group were sacrificed 10 d after treatment and the tumor sizes and body weights were measured.The microvessel density(MVD)was assessed by immunostaining of endothelial cells for CD31 and the cell apoptoses were observed by transmission electron microscope.Another thirty mice were treated for 30 days;the survival period of mice and toxicity of ZD7155 were observed till the 49th day of treatment.Results:Ten days after treatment,the mean tumor volumes in the control,low-dose and high-dose groups were(35.8?6.7)cm~3,(21.5?6.1)cm~3 and (10.7?4.1)cm~3,respectively(P

The structural gene encoding mature peptide of extracellular ? amylase was amplified from the genome DNA of hyperthermophilic archaeon Pyrococcus furiosus by PCR The recombinant plasmid pUC19 amy was constructed by inserting the amplified segment into vector pUC19 The recombinant vector pYX212 amy was constructed by ligate the heterogeneous fragment of pUC19 amy into the multiple cloning site of pYX212, an expression vector of yeast Saccharomyces cerevisiae W303 A1 were transformed with pYX212 amy by electroporation The transformant expressed the activity of the thermophilic ? amylase successfully The recombinant enzyme has the similar enzymatic properties as the extracellular ? amylase produced by Pyrococcus furiosus : it shows an enzymatic activity optimum at pH 5 0, and its optimal temperature for enzymatic activity is about 90℃, more than 50% of its initial enzymatic activity is still detectable after it was incubated at 121℃ for 30 minutes

Biopsy, Fine-Needle ; Carcinoma ; pathology ; Endosonography ; Giant Cells ; pathology ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Osteoclasts ; pathology ; Pancreas ; diagnostic imaging ; pathology ; Pancreatic Neoplasms ; pathology

OBJECTIVETo investigate the changes in the levels of monocarboxylate transporter-2 in spinal cord horn in a rat model of chronic inflammatory pain.

METHODSMale SD rats weighting 180 - 220 g were randomly divided into two groups(n = 48): normal saline group (NS group), complete Freund's adjuvant group (CFA group). Rats were given injections of CFA 100 µl in left hind paw in group CFA, and an equal volume of saline was given injection in group NS. Mechanical withdraw threshold(MWT) and thermal withdraw latency(TWL) were measured at before injection(T0 and 3 h, 1 d, 3 d, 7 d, 14 d, and 21 d after injection(T1-7). Four rats were chosen from each group at T0-7 and sacrificed, and L4-5 segments of the spinal cord horn were removed for measurement of the expression of monocarboxylate transporter-2 by Western blot analysis.

RESULTSIn CFA group, mechanical hyperalgesia and allodynia appeared on the 3 h after CFA injection, then until the day 14. The expression of monocarboxylate transporter-2 in the spinal dorsal horn of rats in CFA group was significantly higher than that in normal control group at T1-6(P <0.05). The protein level of monocarboxylate transporter-2 was apparently correlated with MWT and TWL(P <0.01 and P <0.05) in CFA group.

CONCLUSIONThe level of monocarboxylate transporter-2 in spinal dorsal horn is significantly increased in a rat model of chronic inflammatory pain and the change may involve in the formation and maintenance of central sensitization in spinal cord of chronic inflammatory uain.

Animals ; Disease Models, Animal ; Freund's Adjuvant ; Hyperalgesia ; chemically induced ; Inflammation ; chemically induced ; metabolism ; Male ; Monocarboxylic Acid Transporters ; metabolism ; Pain ; chemically induced ; metabolism ; Rats ; Rats, Sprague-Dawley ; Spinal Cord ; metabolism ; physiopathology