Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Drug-Related Side Effects and Adverse Reactions ; epidemiology ; Female ; Humans ; Infant ; Male ; Middle Aged ; Pesticides ; poisoning ; Young Adult

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; China ; epidemiology ; Drug Overdose ; epidemiology ; Female ; Hospitals, General ; statistics & numerical data ; Humans ; Infant ; Male ; Middle Aged ; Poisoning ; epidemiology ; Young Adult

Animals ; Biomarkers ; metabolism ; Body Fluids ; metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs ; metabolism ; physiology ; Neoplasms ; metabolism ; physiopathology ; Reverse Transcriptase Polymerase Chain Reaction

Animals ; Humans ; Spectrometry, Mass, Electrospray Ionization ; Spectrophotometry, Atomic ; Thallium ; analysis ; metabolism

ObjectiveTo obsrve the clinical efficacy of acupuncture, herbal medicine, plus Ethinylestradiol and Cyproterone Acetate tablets in treating infertility due to obesity-type polycystic ovary syndrome (PCOS).MethodA hundred elegible patients were divided into a treatment group and a control group according to their visiting sequence and random number table. The two groups both received oral administration of Ethinylestradiol and Cyproterone Acetate tables for 3 months. Afterwords, the treatment group was given Chinese herbal medication and acupuncture, while the control group received oral administration of Metforminand Clomiphene plus muscular injection of human menopausal gonadotropin(HMG) and human choionic gonadotropin (HCG). Both groups received a 3-month course. Before and 3 months after the intervention, serum sex hormones were detected, including:follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), estrodiol (E2), and prolactin (PRL); fasting sugar, sugar tolerance, and insulin release were measured; ovulation, pregnance rate, and total effective rate were calculated.ResultAfter intervention, there were significant differences in comparing LH, LH/FSH, ovulation, and the incidence of follicle hyperstimulating syndrome between the two groups (P<0.05); there were no significant differences in comparing sugar metabolism, insulin release, comprehensive efficacy, dominant follicle count, and pregnancy rate(P>0.05). After intervention, the LH and LH/FSH levels were significantly changed in the treatment group, while the LH and LH/FSH levels, ovulation, and dominant follicle count were significantly changed in the control group (P<0.05), but pregnancy rate and follicle hyperstimulating syndrome were insignificantly changed in both groups (P>0.05).ConclusionCombination of acupuncture, Chinese herbal medicine, and Ehinylestradiol and Cyproterone Acetate tablets can down-regulate the LH and LH/FSH levels, andenhance ovulation, without causing follicle hyperstimulating syndrome.

Azabicyclo Compounds ; blood ; Chromatography, Gas ; Humans ; Piperazines ; blood ; Serum ; chemistry

Objective To understand the psychological health status of the middle and primary school students,and to provide some advice of their mental health care.Methods In 11 611 middle and primary school students in 18 schools in Maojian area of Shiyan city,the students,teachers and parents at 5 schods were investigated a randomely,targets were examined by Achenbach Child Behavior Checklist(CBCL),Pupil Rating Scale Revised Screening for Learning(PRS),Conner's Parent Symptom Questionnaire(PSQ)and Conner's Teacher Rating Scale(TRS)and self-established questionnaires for general information.Results The investigation demonstrated:89.29% of students possessed very promising dreams and sublime pursuits,and had correct outlook on life and values;10.71% of students had comparatively severe psychological problems.The major problems appeared to be indefinite purpose of study,shorted of mental endurance and severe impulsivetrend.Moreover,an-xiety,emotion instability and solitary trend caused indifference,sheepish ness and difficulty in their communication.Conclusion Schools,families and society pay close attention to students,mental health and help the youngsters develop in a healthily psychdogical way.

Objective To understand the mechanism of cerebral energy failure after hypoxia ischemia at the molecular level and to establish the protocol for the safe and effective treatment of hypoxic-ischemic encephalopathy(HIE).Methods One hundred neonatal rats were divided into normal control group and hypoxic-ischemic(HI) group. SD rats of both groups were decapitated at the time of 2 h,24 h,48 h,72 h and 7 d after HI.These tissues of cerebrum,cortex and hippocampus were taken out to explore the influence of HI on the expression of GLUT1 and GLUT3 genes with the method of RT-PCR.Results There was an enhancement in the expression of GLUT1 and GLUT3 genes with the increasing of day age. The expression was more intense in hippocampus than that in cortex. However, HI could significantly enhance the expression of GLUT genes. The expression was higher in cortex than that in hippocampus. The expression of two genes reached the peak at 24 h after HI, but was significantly lower than that in control group at 7 d after HI.Conclusion The increased expression of GLUT genes can maintain the energy supplement for the brain and delay a cascade reaction of cerebral energy failure.

Objective To evaluate the efficacy of gentamicin solution in transurethral ureteroscopic lithotripsy to prevent postop- erative infection.Methods Prospective clinical randomized control study was conducted.From July 2003 to June 2006,116 ca- ses of ureteral stones at high risk of postoperative infection were randomized into control group or gentamicin group.Patients in gentamicin group received gentamicin solution for washing in the operation.All the patients undergoing operation were followed up for 2 weeks after operation.Diagnosis of postoperative infection was based on clinical manifestations.Results A total of 109 patients received operation in all the 116 cases,including 58 cases in gentamicin group and 51 cases in control group.Thirteen cases of postoperative infection were identified in all the patients receiving operation (11.93%),3 cases in gentamicin group and 10 in control group.The incidence of postoperative infection was significantly different between the two groups (X~2= 5.3342,P=0.0209).Eight cases had positive bacterial culture.Of the microbiological isolates,2 were gram-positive bacteria, 5 gram-negative bacteria and 1 Candida albicans.Conclusions The most common pathogen causing postoperative infection after transurethral ureteroscopic lithotripsy is gram-negative bacteria.The use of gentamicin solution for washing in the operation can reduce the incidence of postoperative infection.

Lipopeptides produced by Bacillus subtilis JA antagonized a broad spectrum of fungal pathogens. Crude lipopeptides were extracted with methanol from the precipitate which was obtained by adding 6 mol/L HCl to the cell-free culture broth.The crude extract was run on Diamonsil C_(18)column(5?m,250 mm?4.6 mm) in reverse phase HPLC system to purify the lipopeptides.Inhibitory ability and IC_(50)values of lipopeptides towards various microorganisms were determined by agar diffusion method.The results showed lipopeptides exhibited strong inhibitory activity against some important plant pathogenic fungi,including R.solani and F.oxysporum.The ability of B.subtilis JA to antagonize against the growth of the post-harvest pathogen -B,cinerea was tested in vitro.Spore germination of B.cinerea was strongly inhibited in the presence of JA cell suspension.Furthermore,B.subtilis JA can produce antifungal volatiles which strongly inhibited the spore germination and mycelial growth of B.cinerea.As a biocontrol agent,the synergic effect of lipopeptides and volatiles may play a major role in controlling the pathogens by B.subtilis JA.