1.Advance of the origin recognition complex role in cell cycle
Journal of Medical Postgraduates 2004;0(01):-
Studies on ORC in the cell cycle were limited,this paper is to explore origin recognition complex(ORC)in the cell cycle,and elucidate the relation between them.
2.Study on the mechanism of S100A9-induced secretion of vascular endothelial growth factor-A by monocytes
Chunlong LI ; Hong LIAO ; Ming JIANG
International Journal of Laboratory Medicine 2015;(13):1816-1817,1820
Objective To explore the molecular mechanism of S100A9-induced secretion of vascular endothelial growth factor-A (VEGF-A)by monocytes.Methods Peripheral blood specimen were collected from healthy individuals undergoing physical exami-nation and the CD14 + monocytes were purified by using immunomagnetic beads and the expression of the receptor for advanced gly-cation endproducts (RAGE)was detected by flow cytomertry.In vitro CD14 + monocytes were stimulated by S100A9,and anti-RAGE antibody or NK-κB signal pathway inhibitor were pre-incubated for 1 hour and then stimulated by S100A9,the levels of VEGF-A were detected by using enzyme-linked immunosorbent assay.Results The high level of RAGE was expressed by isolated CD14 + monocytes,after S100A9 stimulation,the secretion of VEGF-A by CD14 + monocytes was significantly increased in a dose and time dependent manner.However,the inducing VEGF-A was significantly decreased(P <0.01 ),while pre-treated with anti-RAGE antibody or NK-κB inhibitor (P <0.01).Conclusion S100A9 inducing the secretion of VEGF-A by monocytes and is de-pended on RAGE-NK-κB signal pathway,suggesting that S100A9 might promote angiogenesis.
3.The Clinical Significance of LDH and ?_2-MG Measuring in Patients with non-Hodgkins Lymphoma
Hong JIANG ; Peizhang LI ; Ming LAO
Journal of Medical Research 2006;0(10):-
Objective To evaluate the clinical significance of the serum lactic dehydrogenate(LDH) and ?2-Microglobulin(?2-MG) measuring in N0n-Hodgkins Lymphoma(NHL) Patients.Methods The LDH and ?2-MG are collected from 126 NHL patients and measured by rate method and radioimmunoassay respectively.Results The 2-year survial,4-year survial and alleviation rate of Patientsinnormat LDH and ?2-MG level group were obviously higher than those intevel increasing group.Difference is of significance(p
4.Protective Effects of Neurotrophin-3 on The Excitotoxic Damage of Cochlear Afferent Neurons in Guinea Pigs
Yongquan ZHANG ; Ming JIANG ; Hong SUN
Journal of Audiology and Speech Pathology 2004;0(05):-
0.05). Conclusion 1. Exogenous glutamate application via scala tympani can result in a rapid excitotoxic damage of cochlear typeⅠspiral ganglion cells of guinea pigs. 2. NT-3 can protect typeⅠspiral ganglion cell from glutamate neurotoxicity even applied 60 minutes after the occurrence of glutamate induced cochlear impairment. The result indicates that NT-3 may be an ideal candidate for the treatment of a series of acute neural hearing loss.It may lead to a better outcome when NT-3 gene therapy is actilized.
5.Monostotic fibrous dysplasia of the sphenoid sinus: two cases report and literature review
Yu CHEN ; Hong SUN ; Guangxiang HE ; Ming JIANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;(23):1071-1073
objective:To study the etiopathogenisis、clinical features、diagnosis and treatments of monostotic fibrous dysplasia of the sphenoid sinus. Method:Two cases of monostotic fibrous dysplasia of the sphenoid sinus without any symptoms was reported with relevant literature review.Result: No aggravation was found after 6 months-follow-up.Conclusion:The cranial fibrous displasia has low incidence rate with non-specific symptoms and high rate of misdiagnosis. The monostotic fibrous dysplasia of the sphenoid sinus without any symptom is rarely seen clinically. Imageological examination,for example,CT and MRI,is valuable for the diagnosis of this disease. The histopathological evidence is absolutely necessary to make definite diagnosis. The conservative treatment may be chosen for the asymptomatic cases. Nasal Endoscopic surgery can not only remove the diseased region but also make diagnosis.The long-term follow-up should be carried out in all of these patients.
6.Strengthening Quality Education in the Fermentation Engineering Experiment Teaching
Qun JIANG ; Li-Ming HE ; Xue-Hong ZHANG ;
Microbiology 1992;0(04):-
Some measures were adopted in the fermentation engineering experiment teaching to explore its important role in the quality education.Good effect is achieved in the aspects of cultivating the ability of combining theory with practice,creativeness,thoroughness,and the spirit of hard-working and team coop- eration of the students.
7.The Clinical Significance of Detecting Nonthyroid Specific Antibodies in Patients with Graves' Diseases
Jiang-Hua JU ; Zhi-Hong GAO ; Ming-Cai QIU
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(06):-
Objective To study the level of immunoglobulin and the prevalence of ANA in patients with Graves' diseases(GD).To explore the correlation between GD and other systemic autoimmune disorders.Methods Data of 145 patients with GD and 45 healthy subjects were collected.All cases were detected on the presence of ANA and the level of immunoglobulin,FT3,FT4,and thyroid specific antibodies.Results The presencerate of ANA and the level of IgG in patients with GD were higher than that in healthy controls [(28.28% vs 4.55%);(70.96?26.14 vs 60.41?11.01) mmol/L](P
8.2D-PAGE Analysis of Chinese Rose Leaf Protein Under Heat Shock Stress
Rui JIANG ; Yong-Hong HU ; Chang-Hua JIANG ; Hong-Wei ZHAO ; Shang-Lian HU ; Feng MING ;
China Biotechnology 2006;0(04):-
Proteins extracted from two varieties of Chinese roses leaves were separated by two- dimensional polyacrylamide gel electrophoresis (2-DE) with immobilized pH gradient (IPG). Many difference proteins were isolated with molecular weights ranging 10-30 kDa and pI5-6. Three proteins of high levels observed in a gel were excised and identified using peptide mass fingerprinting and MS-MS. A summary of the identified proteins and their putative functions are presented. They are identified as eIF-5A、LEA protein and Hsp17. 5. Functions of these proteins in plant tolerance to high temperature were discussed.
9.Comparison of liquid-based and conventional preparations in cytologic diagnosis of pancreatobiliary lesions.
Hong JIANG ; Zi-juan QI ; Ming DU ; Xu REN ; He-ming WU ; Lin LIU
Chinese Journal of Pathology 2013;42(4):269-270
Adult
;
Aged
;
Aged, 80 and over
;
Bile Duct Diseases
;
diagnosis
;
Bile Duct Neoplasms
;
diagnosis
;
Cholangiopancreatography, Endoscopic Retrograde
;
Cytodiagnosis
;
Cytological Techniques
;
methods
;
Female
;
Humans
;
Male
;
Middle Aged
;
Pancreatic Diseases
;
diagnosis
;
Pancreatic Neoplasms
;
diagnosis
10.Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis.
Chang-Ming WANG ; Juan CHEN ; Ming JIANG ; Xiu-Ping XUAN ; Hong-Xiu LI
Acta Pharmaceutica Sinica 2014;49(1):142-147
This study is to investigate the effect of artesunate on transforming growth factor-beta1 (TGF-beta1) induced epithelial-mesenchymal transition (EMT) and its possible mechanism. After the in vitro cultured RLE-6TN cells were treated with TGF-beta1 then artesunate intervened on it, after 24 h, expression of the markers of mesenchymal cell was assayed using Western blotting and real-time PCR analysis. Western blotting was also used to detect the effect of TGF-beta1 on the Smad3 and Smad7 expressions of RLE-6TN cells. Morphological alterations were examined by phase-contrast microscope, and ultrastructure changes by electron microscope. Incubation of RLE-6TN cells with TGF-beta1 resulted in the up-regulation of the expression of the mesenchymal cell markers, after artesunate intervened on it, resulted in the down-regulation of the expression. Meanwhile, incubation with artesunate intervened on RLE-6TN cells could lead to the apparent down-regulation of the expression of Smad3 and up-regulation of Samd7 and the transition of RLE-6TN cells to mesenchymal-like by TGF-beta1 induction, after artesunate intervened on it, RLE-6TN cells to epithelial-like. TGF-beta1 induced epithelial-mesenchymal transition process; artesunate can inhibit TGF-beta1-induced epithelial-mesenchymal transition process, the possible mechanism is up-regulation of the expression of Smad7 and down-regulation of the expression of Smad3, meanwhile inhibits phosphorylation of Smad3.
Actins
;
genetics
;
metabolism
;
Animals
;
Artemisia
;
chemistry
;
Artemisinins
;
isolation & purification
;
pharmacology
;
Cell Line
;
Cell Proliferation
;
drug effects
;
Epithelial Cells
;
cytology
;
metabolism
;
Epithelial-Mesenchymal Transition
;
drug effects
;
Idiopathic Pulmonary Fibrosis
;
pathology
;
Plants, Medicinal
;
chemistry
;
Pulmonary Alveoli
;
cytology
;
RNA, Messenger
;
metabolism
;
Rats
;
Smad3 Protein
;
genetics
;
metabolism
;
Smad7 Protein
;
genetics
;
metabolism
;
Transforming Growth Factor beta1
;
pharmacology
;
Vimentin
;
genetics
;
metabolism