1.Generation mechanisms and management strategies of adverse reactions to Bevacizumab during cancer treatment.
Chinese Journal of Oncology 2010;32(7):481-486
Angiogenesis Inhibitors
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adverse effects
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therapeutic use
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Angiotensin-Converting Enzyme Inhibitors
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therapeutic use
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Antibodies, Monoclonal
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adverse effects
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therapeutic use
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Antibodies, Monoclonal, Humanized
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Aspirin
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administration & dosage
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therapeutic use
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Bevacizumab
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Hemorrhage
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chemically induced
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Humans
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Hypertension
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chemically induced
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drug therapy
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Intestinal Perforation
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chemically induced
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surgery
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Neoplasms
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drug therapy
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Proteinuria
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chemically induced
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Thromboembolism
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chemically induced
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drug therapy
2.Clinical Observation of Hs-CRP,IL-6,IL-8,TNF-? in Peritoneal Dialysis Patients
Qunhong XU ; Xiao FEI ; Ming WANG
Journal of Medical Research 2006;0(07):-
Objective To compare the different micro inflammatory states between pafients undergone peritoneal dialysis and the pafients of chronic renal failure without dialysis,and to observe the condition of the occurrence of the micro inflammatory state in the peritoneal dialysis patients.Methods This study included 53 peritoneal dialysis patients who were followed-up in our dialysis center,34 chronic renal failure patients without dialysis and 47 normal controls,hs-CRP,IL-6,IL-8,TNF-? in venous blood were defected with empty stomach,and then compare the number of patients in each group.Results Patients'hs-CRP of the group of the PD is lower than that in the group of the chronic renal failure without dialysis,and higher than that in the normal people(P
3.Clinical effect of bicyclol combined with polyene phosphatidyl choline in elderly non-alcoholic fatty liver disease
Ming LI ; Fan YANG ; Fei XU ; Niansong QIAN
Chinese Journal of Postgraduates of Medicine 2014;37(1):1-3
Objective To observe the clinical effect of bicyclol combined with polyene phosphatidyl choline in elderly non-alcoholic fatty liver disease (NAFLD).Methods One hundred and twenty elderly patients with NAFLD were divided into 3 groups by block randomization method,40 cases in each group.Therapeutic group was treated by bicyclol combined with polyene phosphatidyl choline; bicyclol group was only treated by bicyclol; and polyene phosphatidyl choline group was only treated by polyene phosphatidyl choline.The blood biochemical indexes,liver ultrasound score and clinical curative effect of 3 groups were compared after treated for 24 weeks.Results The total cholesterol (TC),triglyceride (TG),alanine aminotransferase (ALT),aspartate aminotransferase (AST) and gamma glutamine transferase (GGT) in 3 groups after treatment were lower than those before treatment,and the difference was statistically significant (P < 0.05) ; TC,TG and ALT levels in therapeutic group after treatment were significantly lower than those in bicyclol group and polyene phosphatidyl choline group [(1.36 ± 0.84) mmol/L vs.(2.77 ± 1.27),(2.84 ±1.35) mmol/L; (1.32 ±0.71) mmol/L vs.(1.89 ±0.87),(1.92 ±0.90) mmol/L; (38.26 ± 12.75) U/L vs.(57.83 ± 16.67),(62.07 ± 18.16) U/L],and the difference was statistically significant (P < 0.05).The liver ultrasound score in 3 groups after treatment was significantly decreased compared with that before treatment,and the difference was statistically significant (P < 0.05).Liver ultrasound scores in therapeutic group after treatment were significantly lower than those in bicyclol group and polyene phosphatidyl choline group [(2.08 ± 0.93) scores vs.(3.17 ± 1.14),(3.34 ± 1.07) scores],and the difference was statistically significant (P < 0.05).The total effective rate in therapeutic group was significantly higher than that in bicyclol group and polyene phosphatidyl choline group [85.0% (34/40) vs.67.5% (27/40),65.0% (26/40)],and the difference was statistically significant (P <0.05).Conclusions Bicyclol combined with polyene phosphatidyl choline has better clinical effect in elderly patients with NAFLD.It is better than single bicyclol and polyene phosphatidyl choline and worth clinical promotion.
4.Recent development of natural and reconstituted lipoprotein based nano drug delivery vehicles.
Ying XU ; Xue-Feng JIN ; Qi-Neng PING ; Hong-Fei LIU ; Mei CHEN ; Xi-Ming XU
Acta Pharmaceutica Sinica 2014;49(1):23-29
Lipoproteins are biological lipids carriers. The natural and reconstituted lipoprotein based drug delivery systems have been extensively developed in recent years. This article reviews the development of natural and reconstituted low-density lipoprotein and high-density lipoprotein based vehicles in the antitumor area.
Animals
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Antineoplastic Agents
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administration & dosage
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chemistry
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Apolipoproteins B
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administration & dosage
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chemistry
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Drug Carriers
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administration & dosage
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chemistry
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Humans
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Lipoproteins
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administration & dosage
;
chemistry
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Lipoproteins, HDL
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administration & dosage
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chemistry
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Lipoproteins, LDL
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administration & dosage
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chemistry
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Nanoparticles
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Neoplasms
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drug therapy
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Peptides
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administration & dosage
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chemistry
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Pharmaceutical Vehicles
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chemistry
5.Enhancing protective immunity effects of the vaccine against Schistosoma japonicum infection through priming with cocktail DNA vaccines and boosting with cocktail protein vaccines
Yang DAI ; Yinchang ZHU ; Xiaoting WANG ; Jianxia TANG ; Fei LU ; Ming XU ; Yongliang XU ; Xiaohong GUAN
Chinese Journal of Schistosomiasis Control 1989;0(01):-
Objective To enhance the protective immunity effects against Schistosoma japonicum infection by priming with cocktail DNA vaccines and boosting with cocktail protein vaccines in infected BALB/c mice.Methods Plasmids and proteins for immunization were prepared and diluted in no bacterial saline solution to final concentration of 1.5 mg/ml,and mixed with pcDNA3.1-SjC23,pcDNA3.1-SjCTPI,pcDNA3.1-(CDR3)6 plasmid DNAs by equal volume to form the cocktail DNA vaccine,and also mixed with recombinant proteins SjC23-HD,SjCTPI,and NP30 by equal volume to form the cocktail protein vaccine.Seventy female BALB/c mice of 4-5 weeks old were randomly divided into 5 groups(A,B,C,D,E).In Group A(control group),each mouse was immunized with 100 ?l saline solution by intramuscular(i.m.);in Group B(pcDNA3.1 control group),each mouse was immunized(i.m.)with 100 ?l pcDNA3.1 for three times at week 0,3,6;in Group C(pcDNA3.1 and cocktail protein group),each mouse was immunized(i.m.)with 100 ?l pcDNA3.1 for three times at week 0,3,6 and immunized with 100 ?l mixed protein vaccines plus 100 ?l FCA by subcutaneous at week 9;in Group D(cocktail DNA vaccines group),each mouse was immunized(i.m.)with 100 ?l mixed DNA vaccines for three times at week 0,3,6;in Group E(cocktail DNA vaccines plus cocktail proteins),each mouse was immunized(i.m.)with 100 ?l mixed DNA vaccines for three times at week 0,3,6 and immunized with 100 ?l mixed protein vaccines plus 100 ?l FCA by subcutaneous at week 9.Four weeks after the last DNA immunization or two weeks after protein boosting,all the mice were challenged with(40?1)cercariae of Schistosoma japonicum by abdominal skin penetration at the same time.Forty-two days post-challenge,the mice were sacrificed and perfused,and the numbers of recovered worms and eggs in liver were counted.The blood was collected from the tail veins of all the mice two days before the first immunization and challenge,respectively,the serum was prepared for detection of IgG,IgG1 and IgG2a.Two days before the challenge,the spleen cells of two mice from each group were cultured and stimulated with ConA and soluble egg antigen(SEA),and the supernatant was collected for detection of IL-2,IL-4 and IFN-?.Results The worm reduction rates in Group C,D and E were 17.70%,32.88% and 45.35%,respectively,compared with the control group.The worm reduction rates in Group D and E were significantly higher than that in Group C(P
6.Establishment of industry promotion technology system in Chinese medicine secondary exploitation based on "component structure theory".
Xu-Dong CHENG ; Liang FENG ; Ming-Hua ZHANG ; Jun-Fei GU ; Xiao-Bin JIA
China Journal of Chinese Materia Medica 2014;39(20):4065-4069
The purpose of the secondary exploitation of Chinese medicine is to improve the quality of Chinese medicine products, enhance core competitiveness, for better use in clinical practice, and more effectively solve the patient suffering. Herbs, extraction, separation, refreshing, preparation and quality control are all involved in the industry promotion of Chinese medicine secondary exploitation of industrial production. The Chinese medicine quality improvement and industry promotion could be realized with the whole process of process optimization, quality control, overall processes improvement. Based on the "component structure theory", "multi-dimensional structure & process dynamic quality control system" and systematic and holistic character of Chinese medicine, impacts of whole process were discussed. Technology systems of Chinese medicine industry promotion was built to provide theoretical basis for improving the quality and efficacy of the secondary development of traditional Chinese medicine products.
Chemistry, Pharmaceutical
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economics
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standards
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China
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Drug and Narcotic Control
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economics
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Drugs, Chinese Herbal
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chemistry
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economics
;
standards
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Humans
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Medicine, Chinese Traditional
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economics
;
standards
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Quality Control
7.Bioactivity of indolylpiperidine-piperazine derivatives on α1 -adrenoceptor-mediated inotropic response
Sufang LI ; Fei LIU ; Wei LIU ; Yue ZHAO ; Zhizhen LU ; Ming XU ; Youyi ZHANG
Chinese Journal of Pharmacology and Toxicology 2012;26(3):276-281
OBJECTIVE To investigate the blocking activities of a series of potential α1-adrenoceptor (α1-AR) antagonists (Compounds B1 -B9) on α1-AR.METHODS ① A series of potential α1-adrenoceptor (α1-AR) antagonists,indolylpiperidine derivative (IPD) and Compounds B1 -B9,with indolylpiperidine moiety and different substitutes were synthesized through the coupling of indolylpiperidine and piperazine derivatives.② Inotropic responses experiment was used to examine blocking effects of IPD and Compounds B1 - B9 in isolated rat atria by phenylephrine (PE) stimulation.③ Blocking effect of IPD and Compounds B1 - B9 on phosphorylation level of extracellular signal-regulated kinase (ERK) in PE treated HEK293 cells was tested by Western blotting.RESULTS ① Potential α1-adrenoceptor (α1-AR) antagonists with indolylpiperidine moiety and different substitutes were synthesized successfully.② PE caused a dose-dependent inotropic response which was inhibited by pre-incubation of phentolamine (Phen),a non-selective α1-AR antagonist,IPD and Compounds B1,B3,B4,B7,B8 and B9,respectively; IPD and Compounds B4 and B8 caused an obvious rightward shift of inotropic response-curve,the pA2 values for IPD and Compounds B4 and B8 were 6.72 ± 0.21,6.86 ± 0.29 and 6.67 ± 0.19,respectively.③ Phosphorylation level of ERK1/2 was inhibited by pre-incubation with Compounds B1,B2,B3,B5,B6,B7,B8 and B9 or IPD in PE treated α1A-AR stably expressed HEK293 cells; PE-stimulated phosphorylation level of ERK1/2 was inhibited by pre-incubation with Compounds B2,B4,B7 or B8 in α1B-AR stably expressed HEK293 cells.CONCLUSION Compound B4 has a selective blocking activity on α1B-AR,and Compounds B1,B3,B5,B6 and B9 or IPD have a selective blocking activity on the phosphorylation level of ERK1/2.
8.Modified hand-assited laparoscopic splenectomy plus pericardial devascularization for the treatment of cirrhotic portal hypertension
Xiaoming SHUAI ; Gaoxiong HAN ; Junhua CHEN ; Fei XU ; Ming CAI ; Kaixiong TAO ; Guobin WANG
Chinese Journal of General Surgery 2012;27(9):706-709
ObjectiveTo evaluate the safety and efficacy of a modified hand-assited laparoscopic splenectomy (HALS) plus pericardial devascularization for the treatment of cirrhotic portal hypertension.MethodsFrom March 2009 to Dec 2011,modified hand-assited laparoscopic splenectomy plus pericardial devascularization was performed on 47 patients with portal hypertension and liver cirrhosis.We performed HALS first, thenconvertedtototallylarparocopicpericardialdevascularizationduring operation.ResultsAll patients received modified HALS plus pericardial devascularization without convertion to open surgery,the mean operative time was ( 154 ± 32) min,the mean intraoperative blood loss was ( 115 ±73) ml,and the mean postoperative hospitalization was (9.2 ± 1.6) days.The perioperative complications included plural effusion in 3 cases,ascites in 4 cases,pancreatic leakage in 1 case and wound dehiscence in 1case. Therewasnoperioperativemortality.ConclusionsModifiedHALSpluspericardial devascularization is a relatively safe and effective procedure in the treatment of portal hypertension due to liver cirrhosis,it has the advantage of hand-assisted and totally laparoscopic procedures.
9.Expression and significance of calcyclin binding protein in rat brain tissues after traumatic brain injury
Ming LIANG ; Xiaosheng HE ; Zhou FEI ; Xiang ZHANG ; Hongyu XU ; Junli HUO ; Xiaoyan CHEN
Chinese Journal of Trauma 2011;27(6):559-562
Objective To investigate the expression and significance of calcyclin binding protein (CacyBP)in the brain of rat model of traumatic brain injury(TBI).Methods Sixty 60 male SD rats were divided randomly into normal control group (n=10) and TBI group (n=50).The TBI model was created by using lateral head rotation device and subdivided into 6 h,24 h,72 h,7 d and 14 d group (10 rats per group).The expression and distribution of CacyBP in the rat brain was investigated immunohistochemically.The presence of the brown stained particles was considered aspositiveand lack of the stained particles agnegative. Results CacyBP was mainly distributed in the hippocampus,dentate gyrus and cortical neuron cytoplasm.Compared with the high level expression of CacyBP in the normal control group,the expression of CacyBP was decreased to the lowest in the rat brain at 6 h post TBI (P<0.01),became stronger gradually at 24 hours and recovered to normal at day 14,with no statistical difference compared with normal control group (P>0.05). Conclusion The lowest level expression of CacyBP after TBI indicates that CacyBP may play an important role in development of brain injury under effect of difierent mechanisms.
10.Application of GeneScan analysis technique in detection of T-cell receptor gene rearrangement in lymphoma.
Jing ZHANG ; Tai-ming ZHANG ; Fei YANG ; Xu CAI ; Xiao-yan ZHOU
Chinese Journal of Pathology 2013;42(9):615-617
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Female
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Gene Rearrangement, T-Lymphocyte
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Humans
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Lymphoma
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genetics
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Lymphoma, Extranodal NK-T-Cell
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genetics
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Lymphoma, Large-Cell, Anaplastic
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genetics
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Lymphoma, T-Cell, Peripheral
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genetics
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Male
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Middle Aged
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Polymerase Chain Reaction
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methods
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Young Adult