1.Determination of ganciclover in human plasma by reversed-phase high performance liquid chromatography
Ming JIN ; Qiangfeng LI ; Fei HUANG ; Liwei GAO ; Jin LIU
Chinese Journal of Forensic Medicine 2010;25(1):30-32
Objective To develop a method for the determination of ganciclover in human plasma by RPHPLC.Methods Plasma containing ganciclover was extracted with methanol and methylene chloride,qualitative and quantitative analysis was carried out directly.Working curve,linear range,recovery,precision and so on was obtained according to the sample pre-processing method and analysis state.The HPLC method has been taken to investigate the plasma concentration of ganciclover for 12 volunteers.Results The relationship of the peak area of ganciclover concentration in plasma linear within the range of 0.05 μg/mL~1.60 μg/mL(r=0.9999).The lowest detection limit was 0.01 μg/mL(S/N≥13).The intra and inter-day RSD were less than 5.1%respectively.The recovery is about 90.0%~95.4%.Conclusion The established method in the article was shown to be sensitive,accurate and simple for the determination of ganciclover level.It is suitable for clinical detection of ganciclover and forensic medicine and toxicology analysis.
3.Sacral Meningeal Cysts:Evaluation with MR Imaging
Fei WANG ; Bolang YU ; Ming ZHANG ; Chenwang JIN
Journal of Practical Radiology 2000;0(02):-
Objective To study the clinical manifestat ions, MR diagnosis and differential diagnosis of sacral meningeal cyst.Methods 25 patients with sacral meningeal cyst proved by operation and pathology, 9 cases of male, 16 cases of female; the age ranged fro m 18 to 56 years old, average age is 40.3. All cases were performed on MR examin ation. Results The shape of cysts in 19 cases were elliptic,long marsupial shape in 3 cases,irregular in 1 case,string shap in 2 cases.The edge of cysts is clear and the wall of cysts is very thin.MR signal in tensity of fl uid in sacral meningeal cysts exhibits similarity to cerebrospinal fluid,which s hows uniform low signal in T 1WI and high signal in T 2WI.There are 8 cases sc an whose MR signal of cysts fluid is much higher than that of cerebrospinal flui d in T 2WI. In 7 cases thin strip nerve root can be found through the cysts in T 1WI and T 2WI. The antrast enhancement was made in 8 cases and there were no any enhancement both in content and wall of cyst.Conclusion MRI is one of the best examing methods for dia gnosiing of sacral meningeal cyst. Correct mastery of MR manifestations can not only help to give right dignosis, but also provide guidance for choices of clini cal treatment.
4.Diagnosis and treatment of cerebral schistosomiasis:a report of 166 cases
Fei ZHU ; Xin HUANG ; Ming WU ; Weixin JIN ; Kui XIE
Chinese Journal of Schistosomiasis Control 2014;(6):695-696
Objective To discuss the clinical features diagnosis and treatment of cerebral schistosomiasis. Methods A to?tal of 166 patients with cerebral schistosomiasis were treated and their clinical data were collected and analyzed retrospectively. Results In 166 cases of cerebral schistosomiasis the confirmative diagnoses of 156 cases were diagnosed according to the clin?ical manifestation etiology immunology and auxiliary examination CT MRI .In among 74 cases were confirmed by pathologi?cal examination 10 cases were diagnosed through to the diagnostic treatment. Totally 102 patients received the oral medication of praziquantel and they all improved and discharged 14?16 days later 64 patients received the craniotomy and praziquantel medication after the operation and 48 patients significantly improved others did not improve or aggravated. There was no opera?tive mortality. Conclusions Neuroimaging and laboratory tests are valuable in the diagnosis of cerebral schistosomiasis. The praziquantel treatment is selected firstly when the diagnosis was established. However in the case of serious intracranial hyper?tension intractable epilepsy and praziquantel treatment fails the surgical treatment is required.
5.Egr-1 mediates Si0(2)-driven transcription of membrane type I matrix metalloproteinase in macrophages.
Fei, XIANG ; Ming, BAI ; Yang, JIN ; Wanli, MA ; Jianbao, XIN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(1):13-6
The up-regulation mechanism of membrane type I matrix metalloproteinase (MT1-MMP) in macrophages stimulated by silica in vitro and the contribution of early growth response 1 (Egr-1) transcription factor in the gene expression pathway were investigated. Macrophages stimulated by silica were treated with Egr-1 antibody or Egr-1 decoy oligodeoxynucleotides (ODN). The levels of MT1-MMP proteins were determined by Western blot and the expression of MT1-MMP mRNAs was detected by RT-PCR. The results showed as compared with control macrophages, silica-stimulated group showed up-regulated gene expression of MT1-MMP via Egr-1 (P<0.01). Compared with silica-stimulated macrophages untreated with antibody, the cells treated with 5 microg/mL Egr-1 antibody were associated with reduced expression of MT1-MMP protein (P<0.01) and mRNA (P<0.01). Compared with silica-stimulated untransfected group, the Egr-1 "decoy" ODN group was associated with reduction in the expression of MT1-MMP protein and mRNA (P<0.01). It was concluded gene expression of MT1-MMP which may play a critical role in silicosis was up-regulated by silica in macrophages. Egr-1 participated in the expression of MT1-MMP and positively regulated the expression. Both Egr-1 antibody and Egr-1 decoy ODN suppressed the expression of MT1-MMP through the Egr-1 pathway and may become a potential therapeutic tool in the management of silicosis in the future.
6.Enhance the Expression of B. subtillis Fibrinolysis Enzyme by degQ Gene
Ming-Fei JIN ; Xin-Hua ZHU ; Li JIN ; Hui-Fang BIAN ; Zi-Rong WU ;
Microbiology 1992;0(02):-
The degQ gene, amplified from Bacillus subtilis by PCR, was cloned to pUBS (sucrose induced secretion vector). After transformed into DB403, recombination named DB403(pUBSD) was formed. The results of the fermentation showed that degQ gene enhanced the expression of B. subtilis fibrin enzyme. The activity of the enzyme was increased to 2.2 times as the original one. In this article, the effects of different conditions, such as different kinds of sugar, different concentration of sucrose and different induced time were also be investigated and compared.
7.Diagnosis and prediction of lung cancer through different classification techniques with tumor markers.
Guang-jin NIE ; Fei-fei FENG ; Yong-jun WU ; Yi-ming WU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(5):257-261
OBJECTIVETo study which classification model was most suitable for establishing a multi-tumor markers lung cancer prediction model, through established logistic regression model, decision trees model and artificial neural network model.
METHODSRIA analysis, ELISA, spectrophotometry, high-performance liquid chromatography (HPLC) and atomic absorption spectrometry were used to measure the serum CEA, CA125, gastrin, NSE, beta2-MG, Sil-6 receptors, sialic acid, nitric oxide, Cu, Zn, Ca and the pseudo-urine nucleoside of urine samples in lung cancer patients, benign lung disease patients and healthy controls. The lung cancer diagnosis models were established by logistic regression analysis, decision tree analysis and artificial neural network training.
RESULTSThe diagnosis sensitivities of the logistic regression analysis, decision tree analysis and artificial neural network model with 12 tumor markers in lung cancer were 94.00%, 100.00% and 100.00%; the specificity were 100.00%, 98.89% and 100.00%; the total accurate 94.29%, 95.00% and 90.00%, respectively.
CONCLUSIONThe results of three classification models with 12 tumor markers in diagnosis of lung cancer are ideal. Especially the C5.0 decision tree model and the artificial neural network model are more suitable for the prediction and diagnosis of the lung cancer.
Aged ; Biomarkers, Tumor ; analysis ; Decision Trees ; Female ; Humans ; Logistic Models ; Lung Neoplasms ; diagnosis ; Male ; Middle Aged ; Neural Networks (Computer)
8.Effects of forepaw sensorimotor deprivation in early life on spatial learning and memory in rats
Yuan-yuan, ZHANG ; Fei, LI ; Xiao-hua, CAO ; Xing-ming, JIN ; Chong-huai, YAN ; Ying, TIAN ; Xiao-ming, SHEN
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(7):767-771
Objective To explore the effects of forepaw sensorimotor deprivation in early life on hippocampus-dependent spatial reference learning and memory in rats. Methods Newborn SD rats were randomly assigned to experiment group (deprivation of forepaw sensorimotor function, n=53) and control group(n=55). Rats of postnatal day 13 (PN13) in experiment group were seleeted, and models of forepaw sensorimotor deprivation were established by microsurgical technique. Open field tests and Morris water maze tests were performed during the time periods of PN25(PN21-31), PN35 (PN31-39), PN45(PN41-50) and PN60(PN56-64) to evaluate the locomotor activity and spatial reference learning and memory, respectively. Results In open field tests, there was no significant difference in parameters of locomotor activity and exploratory behavior between the two groups (P>0.05). In Morris water maze tests, eontrol group performed significantly better than experiment group during training sessions and probe tests on PN25 and PN35 (P<0.05). While on PN45, although there was no significant difference between the two groups during training sessions, control group performed significantly better than experiment group during probe tests (P<0.05). Conclusion The deprivation of forepaw sensorimotor in early life has no signifieant effect on the locomotor activity and exploratory behavior of rats, but can impair the spatial reference learning and memory.
9.Effect of p38MAPK inhibitor on the soft palate reconstruction of the rats with chronic intermittent hypoxia
Liqiao GE ; Ting MING ; Jing YAN ; Jin HOU ; Nan GONG ; Lin ZHAO ; Fei WANG
Chinese Archives of Otolaryngology-Head and Neck Surgery 2016;23(1):49-52
OBJECTIVE To explore the role of P38MAPK inhibitor on the soft palate reconstruction of the rats with chronic intermittent hypoxia. METHODS The animals were divided into normal control group, hypoxia control group and SB203580+hypoxia group (every group 20 rats). After 5 weeks, the expressions of p38MAPK and p-p38MAPK protein on the soft palate of the rats were detected with immunohistochemical techniques and western blot. RESULTS Compared with the normal control group, the soft palate tissue thickness of the hypoxia group were increased most obviously; Levels of p38MAPK were increased in hypoxia control group; Compared with the hypoxia control group, the levels of p-p38MAPK group were decreased in SB203580+hypoxia group. CONCLUSION p38MAPK may play important roles in the soft palate reconstruction of the rats with chronic intermittent hypoxia.
10.Vacuum sealing drainage combined with free skin graft in repairing cutaneous deficiency of traumatic shank amputation stump.
Xiao-fei ZHAO ; Chun-you LI ; Guo-qiang JIN ; Xiao-feng MING ; Guo-jie WANG
China Journal of Orthopaedics and Traumatology 2014;27(12):1036-1039
OBJECTIVETo observe clinical efficacy in treating cutaneous deficiency of traumatic shank amputation stump with full-thickness skin graft combined with vacuum sealing drainage.
METHODSFrom September 2009 to December 2012, 15 patients with cutaneous deficiency of traumatic shank amputation stump were treated with full-thickness skin graft combined with vacuum sealing drainage. Among patients, there were 11 males and 4 females with an average age of 41.5 (ranged from 25 to 62) years old. Ten cases were caused by traffic accident and 5 cases were caused by heavy object, 9 cases on left and 6 cases on right. Six patients with smashed wound were treated with debridement and amputation, combined with vacuum aspiration in-emergency; 9 patients caused by infection and necrosis were treated with debridement and amputation, combined with vacuum aspiration, and full-thickness skin graft were performed at stage II. The skin defect area of residual limbs ranged from 40 cm x 20 cm to 25 cm x 15 cm.
RESULTSAll patients were followed up from 3 months to 1 year. Full-thickness skin graft of residual limbs were survived,and obtained satisfactory walking function with prosthetic. Residual skin increased thicken, wearproof without rupture and pain.
CONCLUSIONFull-thickness skin graft combined with vacuum sealing drainage in treating cutaneous deficiency of traumatic shank amputation stump could reserve the length of residual limbs, increase survival rate of skin graft with less scar of survival skin, get good wearability and it is conducive to prosthetic wear. It is a simple and easy treatment method.
Adult ; Amputation Stumps ; surgery ; Female ; Humans ; Leg Injuries ; surgery ; Male ; Middle Aged ; Negative-Pressure Wound Therapy ; methods ; Skin ; injuries ; Skin Transplantation