1.Study of Teacher's Work Related Stress in the Special Education Field (review)
Chinese Journal of Rehabilitation Theory and Practice 2006;12(12):1083-1085
The author reviewed some researches on teacher's work related stress in China and abroad. The present researches mainly focus on the sources of stress in teachers' work life. Some practices of work related stress in USA and Chinese special education field were discussed in the article. In the end, the author took a perspective on future research of teacher's work related stress in Chinese special education field.
3.Effect of tissue adhesive Histoacryl on cracked wounds on children's craniofacial skin
Ying XU ; Ming CHENG ; Jie GU
Modern Clinical Nursing 2017;16(5):31-34
Objective To compare the effect of the tissue adhesive Histoacryl and traditional suturing treatment on the cracked wounds on children's craniofacial skin. Methods About 152 children patients with cracked wounds children's craniofacial skin from March 2015 to June 2015 were divided into two groups according to their parents' will. Group A, with 77 patients, was treated by the tissue adhesive Histoacryl and Group B, with 75 patients, was treated by traditional suturing. The two groups were compare in terms of the treatment time and effect. Result The treatment time of Group A was significantly shorter than that of Group B (P<0.001), while there was no significant difference between the two groups in the level of wound healing (P>0.05). Conclusions Compared to the traditional suturing, the tissue adhesive Histoacryl is advantageous, for its easy operation, short treatment time, prominent efficacy and beautiful appearance. Moreover, it can relieve the pain of children during the treatment.
4.Mechanisms of inhibiting the proliferation of triple negative breast cancer cell lines by silencing the CD147 gene
Cheng WANG ; Zhigang ZHUANG ; Ming SHAN ; Ming XU
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(3):305-311
Objective · To explore the inhibitive effect of silencing the CD147 gene on the proliferation of triple negative breast cancer cells and relevant mechanisms. Methods · Normal human mammary epithelial cell line HMEC and three triple negative breast cancer cell lines MDA-MB-231, HCC70 and T4-2 were cultured in vitro. mRNA and protein expressions in cells were measured using realtime-PCR and Western blotting, respectively. A siRNA sequence targeting the coding region of human CD147 gene was designed and used to construct a recombinant lentivirus Lv-shRNA-CD147, which was used to infect the three breast cancer cell lines. The negative control group (Lv-NC infected group) and the noninfected cell control group (cell control group) were simultaneously used. The effects of silencing the CD147 gene were measured with real-time PCR and Western blotting. The proliferation and migration of cells were measured with MTT and Transwell assay, respectively. HCC70 cells were collected 72 h after viral infection and proteins related to proliferation, migration and apoptosis of cells (β-catenin, MMP2, MMP9, and Bax) were measured with Western blotting. Results · mRNA and protein expressions of CD147 were significantly higher in three breast cancer cell lines than in HMEC (P<0.01). The Lv-shRNA-CD147 infected group had lower mRNA and protein expressions of CD147, cell proliferation, and cell migration as compared with the Lv-NC infected group and the cell control group,the differences were statistically significant (P<0.01). The Lv-shRNA-CD147 infected group had lower expressions of β-catenin, MMP2, and MMP9, and higher Bax expression in HCC70 cells 72h after viral infection as compared with the Lv-NC infected group and the cell control group, the differences were statistically significant (P<0.01). Conclusion · Silencing the CD147 gene can inhibit the proliferation and migration of triple negative breast cancer cells.
5.Effect of Tanshinone ⅡA on nerve conduction of oxaliplatin induced peripheral neuropathy in rats
Weiting CHENG ; Kai XU ; Zuowei HU ; Shan WANG ; Ming XIA
The Journal of Practical Medicine 2014;(21):3411-3414
Objective To investigate the effect of TanshinoneⅡA on nerve conduction of oxaliplatin induced peripheral neuropathy in rats. Methods Fifty Wistar rats were randomly divided into normal control group, model group, treatment group, prevention group, prevention and treatment group. Except for those in model group, Wistar rats were injected i.p. with oxaliplatin (20 mg/kg). The electrophysiological instrument were employed to detect the sciatic nerve conduction velocity, latency, amplitude 6 h, 24 h, 72 h and 7 d after modeling. Results In the model group, velocity of sciatic nerve conduction slowed, and latency prolonged 24 h after modeling (P<0.05) which continued to slow and prolong until 7 d after modeling (P<0.05). After the application of Tanshinone ⅡA, nerve conduction velocity became faster and latency shorter significantly (P <0.05), especially in the prevention and treatment group, in which no significant difference was found when compared with those in the normal control group (P > 0.05). Conclusions During the chemotherapy with oxaliplatin , TanshinoneⅡA can increase the conduction velocity of sciatic nerve , shorten the disease duration and play a protective role for peripheral nerve.
6.Clinical Characteristics of Norovirus infection with Acute Viral Diarrhea of Yangzhou City
Youjiang ZHANG ; Ming CHENG ; Jianguo HOU ; Jin ZHANG ; Qin XU
Journal of Modern Laboratory Medicine 2014;(5):80-82
Objective To investigate the clinical and epidemiological characteristics in 55 patients with Acute Viral Diarrhea caused by Norovirus.And provide basis for preventing and controlling the spread of the disease.Methods A total of 5 1 3 fe-cal samples of diarrhea cases from 3 surveillance hospitals in 2013 from January to December were collected,analyzed the clinical date and detected Norovirus useing RT-PCR.Results The number of Norovirus positive was 55,positive rate was 10.7%.There were significant differences between Norovirus infection and not infected patients for age (χ2=4.27,P<0.05),then there were no significant differences between Norovirus infection and not infected patients for gender,employ-ment,whether food pathogenic diseases (χ2=0.086~3.21,P>0.05).The clinical symptoms of acute gastroenteritis pa-tients caused by Norovirus infection was fever (χ2=4.6,P<0.05),there were no significant differences for diarrhea (≥5 times/day),bellgache,vomiting,nausea (χ2=0.139~3.636,P>0.05).Conclusion Norovirus is common pathogens of a-cute diarrhoea,which incidence is higher.Clinical symptoms alone is difficult to differentiate with other diarrheaphases.The confirmed diagnosis is depended on the etiology.
7.Clinical value of ultrasonography in typing epididymal tuberculosis
Ruiming CHENG ; Zhaoming LIU ; Ming ZHANG ; Jing XU
Chinese Journal of Ultrasonography 2016;25(2):163-167
Objective To investigate the value of ultrasound in typing of epididymal tuberculosis on the clinical treatment . Methods The sonographic characteristics and treatment outcomes were analyzed in 125 patients with epididymal tuberculosis . Results In 125 patients with epididymal tuberculosis ,5 types were divided:( 1 ) Diffusive type ( 37 cases ) presented images of the epididymal diffuse enlargement , abnormal shape ,not smooth surface and inhomogeneous reduced in echogenicity ,13 cases were treated conservatively and 24 cases underwent surgical treatment after failure of conservative treatment . (2) Mass type (24 cases) showed images of hypoechoic mass lesion of caput epididymidis or cauda epididymidis ,4 cases were treated with surgical operation and 20 cases were treat with conservative therapy . (3) Abscess type ( 33 cases) manifested images of irregular liquid anechoic of caput epididymidis and cauda epididymidis , poor ultrasound penetration ,and unclear border . (4) Ulcer‐fistula type (9 cases) revealed images of liquid hypoechoic caput epididymidis or cauda epididymidis extend to the surface of the scrotum . ( 5) Testicular involvement type (22 cases) detected inhomogeneous hypoechoic mass lesion within the testicle and unclear border . The patients of abscess type ,ulcer‐fistula type and testicular involvement type were all treated surgically . Conclusions Ultrasonic diagnosis of epididymal tuberculosis provided a reliable basis for clinical treatment scheme and it was worth recommending .
8.Application of ISSR-PCR to identification of different Dendrobium Sw. species
Ying SHEN ; Cheng XU ; Xiaofeng WAN ; Ming ZHANG
Chinese Traditional and Herbal Drugs 1994;0(03):-
Objective To identify nine species of Dendrobium Sw. by inter-simple sequence repeats-polymerase chain reaction (ISSR-PCR) method and to investigate the difference among the species of Dendrobium Sw. at DNA molecular level. Methods Ten primers constituted by simple sequence repeats (SSR) were tested for PCR and sepharose electrophoresis of the nine species. Results Seven of the ten primers amplified polymorphic bands. The number of polymorphic band positions of each primer ranged from 7 to 14, and the size of fragments ranged from 220 to 1 260 bp. The amplification patterns of two primers, namely UBC-807 and UBC-864, were higher in terms of polymorphic and amplified band ratio. Each of them was able to distinguish all the examined species. Conclusion ISSR-PCR provides a quick, reliable molecular marker technique for identification of different species of Dendrobium Sw.
9.Clinical research on advanced liver cancer treated with percutaneous RFA cool-tip electrode under ultrasound guidance
Shixi CHENG ; Guowen YING ; Weidong XU ; Wei XI ; Ming ZHANG
Journal of Interventional Radiology 1992;0(01):-
Objective To evaluate the clinical efficacy and complications in the treatment of advance liver cancer under ultrasound guidance with percutaneous RFTI-1 TM-cold cycle RF tumor ablation apparatus produced by Nangjing TianMa high-tech Company limited. Methods 25 patients of advance liver cancer, including 8 cases of metastatic liver cancer, 17 of primary hepatic carcinoma, were treated with percutaneous RFA cool-tip electrode under Ultrasound guidance 1 to 2 times. Eight of the patients prior to RFA were treated with TACE of individually three times for each. The postoperative efficacy was evaluated by enhanced CT. Results 10 lesions were completely necrotized and the majority parts of another 15 lesions were also under necrosis. Follow-up of six months, all patients are still alive with marked improvement of life quality. Conclusion The short-term efficacy of percutaneous Ultrasound-guided RFA with cool-tip electrode for treating advance liver cancer is quite satisfied, worthy to be recommended.
10.Meta analysis of comparison between atropine and cyciopentolate in cycloplegia
Shi-ming, CHENG ; Xia, ZHOU ; Yan, LI ; Ling, XU
Chinese Journal of Experimental Ophthalmology 2012;(12):1135-1138
Background Medical refraction after cycloplegia is the preferable choice for precise measurement of degree of refractive error.Drugs used in China for cycloplegia include atropine and tropicamide,and the use of cyclopentolate is an alternative for ophthalmologist.However,the data for the evaluation and comparison of efficacy of the available drugs in cycloplegia is still lacking.Objective This system analysis was to evaluate the difference between atropine and cyclopentolate in cycloplegia in children.Methods A systematic literature retrieval was conducted in MEDLINE,EMbase,Google residual accommodation after cycloplegia by atropine and cyclopentolate were compared.Statistical analysis was performed using the RevMan 5.1.0 software.Results A total of 7 studies were included in this meta analysis,including 6 cohort study design and 1 randomized,doubleblinded clinical trial and 1232 eyes.For retinoscopic evaluation after cycloplegia,no significant differences were found between cyclopentolate and atropine in children with hyperopia and myopia (WMD =-0.21,95% CI:-0.47-0.06,P=0.13 ; WMD =-0.10,95% CI:-0.36-0.15,P =0.43).For residual accommodation after cycloplegia,no significant difference was seen between cyclopentolate and atropine in ammetropic children (WMD =0.30,95% CI:-0.10-0.71,P =0.15).Conclusions Cyclopentolate shows the same effect on the cycloplegia as atropine in children,and it can take the place of atropine in cycloplegia in childhood.