1.Neutrophil to lymphocyte ratio and its relationship with the chemotherapy effect and the prognosis in patients with diffuse large B-cell lymphoma
Ming ZHU ; Yanli LI ; Zhitao WANG ; Qianshan TAO ; Zhimin ZHAI
Journal of Leukemia & Lymphoma 2015;24(8):460-463
Objective To explore the neutrophil to lymphocyte ratio (NLR) and its relationship with the effect of chemotherapy and prognosis in patients with diffuse large B-cell lymphoma (DLBCL).Methods The clinicopathological characteristics and outcome of 51 patients with DLBCL diagnosed by pathological biopsy and immunohistochemistry who received CHOP or R-CHOP regimen were collected and reviewed.According to the median of NLR,the patients were divided into low NLR group (NLR≤2.32) and high NLR group (NLR>2.32).The prognostic influence of the NLR on overall survival (OS) was studied by Kaplan-Meier method and Log-rank test.To evaluate the independent prognostic relevance of NLR,univariate and multivariate Cox regression models were applied.Results The complete response (CR) rates of the low and high NLR groups were 71.4 % (20/28) and 39.1% (9/23),respectively (P =0.02).The OS in the low NLR group was significantly better than that in the high NLR group (1,2 and 3-year OSs were 96.4 %,90.4 % and 72.3 % vs 63.9 %,52.7 % and 42.2 %,respectively,P =0.009).Univariate and multivariate Cox regression models analysis showed that NLR > 2.32 was an independent prognostic factor (P =0.016).Conclusion An elevated NLR before treatment indicates the poor effect of chemotherapy and prognosis of patients.NLR is an independent prognostic factor for DLBCL.
2.The research actualities and developing trend of nitric oxide (NO) inhalation systems.
Hong-tao ZHANG ; Zhong-ming LIU ; Xue-feng ZHU ; Jiang-tao WANG
Chinese Journal of Medical Instrumentation 2005;29(4):289-291
This paper discusses the limitations of current NO inhalation systems, based on the research in collocation of NO, inspection of NO/NO2 and synchronous working of NO inhalation systems with ventilators. And then, the developing trend of NO inhalation systems is put forward here too.
Administration, Inhalation
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Nitric Oxide
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administration & dosage
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Ventilators, Mechanical
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trends
3.Primary synovial sarcoma of pericardium: report of a case.
Li-yang TAO ; Miao-xia HE ; Chen-guang BAI ; Hui JIANG ; Ting FENG ; Jian-ming ZHENG ; Ming-hua ZHU
Chinese Journal of Pathology 2012;41(10):704-705
12E7 Antigen
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Antigens, CD
;
metabolism
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Cell Adhesion Molecules
;
metabolism
;
Diagnosis, Differential
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Fibrosarcoma
;
metabolism
;
pathology
;
Heart Neoplasms
;
genetics
;
metabolism
;
pathology
;
surgery
;
Humans
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Male
;
Mesothelioma
;
genetics
;
metabolism
;
pathology
;
Middle Aged
;
Mucin-1
;
metabolism
;
Oncogene Proteins, Fusion
;
metabolism
;
Pericardiectomy
;
Pericardium
;
pathology
;
Sarcoma
;
metabolism
;
pathology
;
Sarcoma, Synovial
;
genetics
;
metabolism
;
pathology
;
surgery
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Translocation, Genetic
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Vimentin
;
metabolism
4.Inhibition of cardiac hypertrophy by mitofusin 2 in neonatal rat cardiomyocytes
Chunlei CHEN ; Tao SHEN ; Ming ZHENG ; Yanhong GUO ; Xiaojun ZHU ; Guanghui CHEN
Journal of Peking University(Health Sciences) 2003;0(05):-
Objective:To study the expression and function of Mitofusin 2(Mfn2)in cultured neonatal rat cardiomyocytes during PE-induced hypertrophy.Methods:The hypertrophy neonatal rat cardiomyocytes model was induced by 0.01 mol/L PE.RT-PCR and Western blot were applied to assess Mfn2 mRNA expression and protein level respectively.Cultured neonatal rat cardiomyocytes were treated by PE after Ad GFP or Ad Mfn2 infection,the protein synthesis was determined by 3H-leucine incorporation assay.Results:PE led to ANF mRNA level(by~1 fold,P
5.Comparative study on different methods for quantitative analysis of oral common microorganisms
ming-zhu, ZHANG ; chao-lun, LI ; yun-tao, JIANG ; wei, JIANG ; jing-ping, LIANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(02):-
flow cytometry.A strong linear relationship was observed in the standard curve of real-time PCR of each bacteria. Conclusion These three non-culture methods can be used in the quantitative analysis of oral microorganisms.Real-time PCR and laser scanning confocal microscopy are better than the traditional culture-based CFU count,and real-time PCR is the most sensitive method.
6.Differential gene expression between Streptococcus sobrinus 6715 and its fluoride-resistant strains
wei, JIANG ; jing-ping, LIANG ; chao-lun, LI ; yun-tao, JIANG ; ming-zhu, ZHANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(02):-
Objective To detect the differential gene expression between Streptococcus sobrinus(S.sobrinus) 6715 and its fluoride-resistant strains. Methods The fluoride-resistant strains of S.sobrinus 6715 was induced by increasing the concentration of fluoride step by step.Total RNA of both S.sobrinus 6715 and its fluoride-resistant strains was extracted,mRNA was separated and purificated,and then cDNA was obtained by reversed transcription.Suppression subtractive hybridization(SSH) technology was used to detect the differential gene expression between them.The differential gene expression fragments were cloned and compared with the GenBank by BLAST.Results After comparing with the GenBank by BLAST,it was identified that there were two differential gene expression fragments,fruA and SMU.438c. Conclusion The cDNA subtractive lib of differential gene expression between S.sobrinus 6715 and its fluoride-resistant strains was successfully constructed through SSH,which paves a way for the further study of fluoride-resistant mechanism.
7.Establishment and application of medical method for quantification of genomic DNA methylation in methotrexate drug resistance cells
Ming LI ; Shilian HU ; Xiaodong HE ; Shaoneng TAO ; Lin DONG ; Yuanyuan ZHU ; Jianfeng WU ; Zuojun SHEN
Chinese Journal of Laboratory Medicine 2009;32(2):204-208
Objective To establish a rapid and convenient method for determination of genomic DNA methylation in cells.Methods Five standard substances (dC, mdC, dA, dT and dG) were separated by high-performance capillary electrophoresis.Bare fused-silica capillary was used and eletrophoresis buffer was 48 mmol/L NaHCO3 with 60 mmol/L SDS, pH 9.6.The temperature of separation was controlled at 25 ℃ and a voltage of 20 kV was applied.The separation of the mixture was performed at a wavelength of 256 nm with UV-Vis detection and injection time was 5 seconds at 0.7 psi.Under optimal condition,genomic DNA methylation in methotrexate drug-resistant A549 cells was detected.Results The optimal condition was made by adjusting SDS concentration(40, 60, 80 mmol/L), pH value of running buffer(9.4,9.6, 9.8), voltage(15, 17, 19, 20, 22 kV), injection time(5, 10, 15, 20, 30 s) and capillary temperature(15, 20, 25, 30 ℃).The method for determination of genomic DNA methylation in cells was established.Five substances were completely separated by high-performance capillary electrophoresis in 10 mins.Intra-day coefficient of variation was less than 0.2% and inter-day coefficient of variation was less than 2%.The minimal detection limit was 2 μmol/L.Percentage of mdC in A549 parent cells was (4.80 ±0.52) %.Percentage of mdC in 15, 30, 40 μmol/L methotrexate drug-resistant A549 cells were (4.20±0.44) %, ( 3.70 ± 0.36 ) %, (3.10±0.35 ) %, respectively.Conclusions Genomic DNA methylation can be quantificated by high-performance capillary electrophoresis efficiently, rapidly, conveniently and sensitively.Genomic DNA methylation in methotrexate drug resistance cells decreases significantly.
8.Detection of folylpolyglutamate synthetase in methotrexate enantiomer induced resistant A549 cells by capillary electrophoresis-laser-induced fluorescence
Renzhi FAN ; Xiaodong HE ; Yuanyuan ZHU ; Ming LI ; Shaoneng TAO ; Lin DONG ; Zuojun SHEN
Chinese Journal of Laboratory Medicine 2008;31(8):924-928
Objective To establish a method for detection folylpolyglutamate syntbetase (FPGS),explore the change of FPGS in the drug-resistant A549 cells induced by methotrexate(MTX) enantiomer,and provide new tools to further investigate drug resistant mechanism. Methods A549 cell lines induced by L-( + )-MTX and D-( - )-MTX (25 μmol/L) were chosen to raise three cell lines as compared with MTX-sensitive cell line. Then FPGS were extracted for the CEIA-LIF and western blot was performed. After validation, FPGS antibodies were labeled by fluoreacein isothiocyanate (FITC) and produced a immune response with former-extracted FPGS. CELA-LIF can separate and detect labeled proteins according ruination time of the protein with different size and detect FPGS in drug resistant cell lines induced with L-(+)-MTX and D-(- )-MTX. The accuracy was evaluated as compared with western blot assay. Results The separation time of CEIA-LIF for labeled FPGS antibody and the immune complexes were7. 1 min and 8.9 min, and the resolving power was 4. 5. The process of protein separation and detection can be accomplished in less than 10 minutes. Western blot analysis showed there was no non-specific bands appears in the extract of these three cell lines after the freeze-thaw in liquid nitrogen. The minimum detection level in sensitive cell strains was 0. 68 mg/μl. The consent of FPGS in L-(+)-MTX and D-( - )-MTX induced cells were 46. 59% and 48. 36% compared with drug sensitive cell strains with CELA-LIF. Conclusions CELA-LIF was established in this experiment. It is efficient and sensitive for detecting of FPGS, which is similar to western blot method. The level of FPGS in L-( + )-MTX and D-( - )-MTX induced drug resistant cell lines is significantly lower, indicating the expression of FPGS is damaged.
9.Correlation between upper airway MRI parameters and the severity of patients with obstructive sleep apnea hypopnea syndrome
Chuanchuan LIU ; Zhichun HUANG ; Ming YANG ; Bin LIU ; Feng TAO ; Xin ZHU ; Xiaoqiong DING ; Ting CHEN
Chinese Archives of Otolaryngology-Head and Neck Surgery 2016;23(4):201-204
[ABSTRACT]OBJECTIVETo investigate the relationship between upper airway MRI parameters and the severity of patients with obstructive sleep apnea hypopnea syndrome (OSAHS). Then the values of upper airway MRI parameters in waking state were assessed.METHODS The MRI upper airway parameters of 37 OSAHS patients and 18 nonsnoring normal subjects were measured and compared. Then data analysis were carried out to find out significant correlations between the MRI upper airway parameters and the severity of patients (the apnea hypopnea index, AHI; the lowest oxygen saturation, LSaO2; the oxygen desaturation index, ODI) with OSAHS.RESULTSThe correlation between cross-sectional areas and left-right diameter in the narrowest retropalatal region, the thickness of lateral pharyngeal wall, the length of the tongue, the length and thickness of the soft palate and the cross-sectional areas of the soft palate on midsagittal planes and severity of OSAHS were found (P<0.05).CONCLUSIONThe MRI upper airway parameters can be used in evaluating the severity of OSAHS in some ways, locating the obstruction sites and choosing the best treatment planning.
10.Cost-effectiveness Analysis of Risperidone and Quetiapine in the Treatment of Schizophrenia
Jianqing TAO ; Jie SHI ; Hong KANG ; Jia LIANG ; Ming YE ; Lan ZHU ; Qiang ZENG
China Pharmacy 2001;0(09):-
OBJECTIVE:To study the econamics effect of risperidone and quetiapine in treating of schizophrenia.METH ODS:64schizophrenia patients were randomly divided into risperidone group(34cases)and quetiapine group(30cases).The effects were evaluated based on the reduction of scores determined by brief psychiatric rating scale(BPRS);the adverse effects were evaluated by treatment emergent symptom scale(TESS).And then the cost-effectiveness analysis was carried through.RESULTS:The per capita costs for risperidone and quetiapine were3598.85yuan and3778.63yuan respectively;the re-duction of BPRS scores were(36.09?15.52)and(25.03?14.45)respectvely;the respective cost-effect ratio was99.72yuan and150.96yuan;the increment of the cost-effect ratio for the quetiapine group was-16.25compared with the risperidone group.CONCLUSION:Risperidone is more economical than quetiapine in the treatment of schizophrenia.