1.Effect of static magnetic field on the proliferation and differentiation of rat osteoblasts
Lihong QIU ; Yang BAO ; Ming ZHONG
Journal of Practical Stomatology 2000;0(05):-
Objective:To Study the effect of static magnetic field on the proliferation and differentiation of osteoblasts.Methods:In vitro cultured rat calvarial osteoblasts were seeded into 96-well culture plate and exposed to the different magnetic intensity field for 24,48 and 72 h respectively.MTT assay was applied to study the cell proliferation. ALP was measured by a microplate reader.Results:When the magnetic exposure time extended to 48 h or 72 h, the corresponding MTT value of osteoblasts with magnetic treatment of 40 mT or 62 mT increased obviously(P0.05). ALP activity increased after 24 h exposure to static magnetic field, the effect of static magnetic field intensity of 62 mT and 83 mT showed the strongest promotion effect(P
2.Clinical analysis of seven acute phosphine poisoning.
Tao CHEN ; Ran SHI ; Xue-zhong YANG ; Xue-zhong YANG ; Ming-jiang QIAN ; Hua-jun CHEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(3):223-225
Acute Disease
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Adult
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Humans
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Male
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Occupational Diseases
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diagnosis
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therapy
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Phosphines
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poisoning
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Poisoning
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diagnosis
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therapy
3.Experimental comparative study of CTA and DSA in detecting delayed cerebral vasospasm in rabbit model
Yong WANG ; Ming ZHONG ; Xianxi TAN ; Yunjun YANG ; Weijian CHEN
Journal of Interventional Radiology 2006;0(09):-
Objective To analyze the accuracy,sensitivity and safety of multidetector CT angiography(MDCTA) and DSA;furthermore to explore the clinical value of MDCTA in studying the delayed cerebral vasospasm(DCVS) .Methods Delayed cerebral vasospasm was induced in 17 rabbits by injection of autologuous blood into the cisterna magna and followed by a second injection 24 hours later.MDCTA and DSA were carried out at the 7th day before and after the procedure in order to obtain the data of vascular diameter changes for comparative study.Results The basilar artery diameters detected by MDCTA were shown preoperatively as(1.55 ? 0.14) mm and postoperatively as(0.95 ? 0.20) mm;and detected by DSA as(1.61 ? 0.19) mm and(1.00 ? 0.17) mm postoperatively;showing statistically equivalence between the two methods.Conclusions MDCTA is recommended as a reliable,rapid,and minimally invasive diagnostic method,providing a new technique for the delayed cerebral vasospasm research.
4.Three-dimensional finite element analysis of stress distribution in necrotic femoral head before and after tantalum rod implantation
Gang ZHU ; Ligui ZHANG ; Zhong ZHENG ; Mingjie XU ; Ming YANG
Chinese Journal of Tissue Engineering Research 2016;20(26):3883-3889
BACKGROUND:Tantalum rod implant technology is a new method of early osteonecrosis treatment. Current research on stress distribution before and after tantalum rod implant in different sizes of femoral head necrosis area is few. OBJECTIVE:To analyze the stress distribution before and after tantalum rod implantation in different sizes of necrotic femoral head area using three-dimensional finite element method. METHODS:Three-dimensional finite element models of normal femoral head and necrotic femoral head of 15, 20 and 30 mm diameterwere constructed. Eight measuring points were chosen on two tiers of each necrotic model to detect the stress distribution and its alteration before and after tantalum rod implantation. RESULTS AND CONCLUSION:(1) Stress concentration werefound on every necrotic femoral head, most pronounced on the one with 30 mm lesion. (2) Tantalum implant appeared to reduce the stress concentration generaly. Comparison of the peak points of these models indicated most significant benefit in 15 mm lesion, next in 30 mm lesion, last in 20 mm lesion. (3) Results indicate that larger lesion entails more concentrated stress distribution and more likely to colapse. Tantalum rod implantation can delay the development of necrosis of the femoral head, andismost effective in smal lesion.
5.Investigations on the audible third heart sound subjects under stress state.
Li-sha ZHONG ; Xing-ming GUO ; Yong YANG ; Shou-zhong XIAO
Chinese Journal of Applied Physiology 2010;26(2):255-256
Exercise Test
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Female
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Heart Sounds
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physiology
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Humans
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Male
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Phonocardiography
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methods
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Pregnancy
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Stress, Physiological
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physiology
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Young Adult
6.Cloning and Expression Analysis of two Endosperm Promoters in Rice
Yong-Lan CUI ; Xiao-Li ZHONG ; Yong-Ming ZHANG ; Zhong-Nan YANG ;
China Biotechnology 2006;0(08):-
The cloning of promoter is important for studying the genetic engineering and the regulation of gene expression in plants. Two promoters Os772 and Os359, which are predicted to be highly expressed in the endosperm of rice from the EST database were cloned. After construction of the Os772∶∶GUS and Os359∶∶GUS expression vectors, they were transformed into rice. X-Gluc staining of transgenic plants showed that Os772 and Os359 can promote GUS gene expression in matured endosperm but not in root, stem, leaf and flower. This result indicates Os772 and Os359 are two rice endosperm-specific promoters.
7.A multilevel analysis on the association between socioeconomic status and psychiatric disorders
Ya-Ming GU ; Fang-Zhong XU ; Ting-Zhong YANG ; Lu LI
Chinese Journal of Epidemiology 2010;31(2):171-174
Objective To explore the association between psychiatric disorders and socioeconomic status such as education,income,employment and area of community settings in Zhejiang province.Methods Data were from the epidemiological survey of mental illnesses aged 15 and older in 2001(n=14 632).Psychiatric disorders were measured by GHQ-12 with a cut-point 4.Logistic multilevel analysis was the main statistical method being used.Results The overall prevalence of psychiatric disorders was 18.5%(95% confidence intervals,17.9% to 19.1%),higher than the data from Hebei province(u=4.39,P<0.001).Annual household income and employment were strongly associated with psychiatric disorders especially for those with low income(odds ratio 3.45,95%CI:1.72-6.67)and unemployment(odds ratio 2.03,95%CI:1.73-2.40).Factors as regional effect and education level showed weak or inconsistent association with psychiatric disorders after controlling on other indicators.Conclusion Inverse gradient relation between psychiatric disorders and SES seemed as the main result of low SES individual characteristics and some minor effects at the county level.
8.Inhibitory effects of Porphyromonas endodontalis lipopolysaccharides on proliferation and differentiation of mouse osteoblast.
Ren LI ; Li-hong QIU ; Di YANG ; Ming XUE ; Ming ZHONG
Chinese Journal of Stomatology 2011;46(3):162-164
OBJECTIVETo observe the effect of lipopolysaccharides (LPS) extracted from Porphyromonas endodontalis (Pe) on osteoblast cell proliferation and the activity of alkaline phosphatase (ALP) and interleukin (IL)-6 secretion and to investigate the role of Pe-LPS in osteoblast proliferation and differentiation.
METHODSMC3T3-E1 cells were treated with different concentrations of Pe-LPS (10, 25, 50 mg/L) respectively. The relative growth rate (RGR) was detected by methyl thiazolyl tetrazolium (MTT) at different time point (12, 24, 48, 72 h). MC3T3-E1 cells were also stimulated with 10, 25 or 50 mg/L Pe-LPS for 6, 12, 24 and 48 h. The activity of ALP was detected by enzyme kinetics assay and the secretion of IL-6 was detected by enzyme-linked immunosorbent assay (ELISA).
RESULTSAfter the stimulation with 25 or 50 mg/L Pe-LPS for 72 h, the RGR of MC3T3-E1 cells descend to 87.46% and 71.12%. The ALP activities of MC3T3-E1 cells were inhibited obviously (P < 0.05) after the stimulation of different concentrations (10, 25, 50 mg/L) Pe-LPS for more than 24 hours. ELISA result showed that IL-6 increased to 32.21 ng/L treated with the 25 mg/L Pe-LPS for 6 h, 25 mg/L Pe-LPS gradually increased the expression of IL-6 from the ELISA results.
CONCLUSIONSPe-LPS can induce the secretion of IL-6 in MC3T3-E1 and decrease the ALP activities of MC3T3-E1, the differentiation of osteoblasts was inhibited. with the long-time toxicity action of Pe-LPS, the proliferation rate of MC3T3-E1 also markedly decreased.
Alkaline Phosphatase ; metabolism ; Animals ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Interleukin-6 ; secretion ; Lipopolysaccharides ; isolation & purification ; pharmacology ; Mice ; Osteoblasts ; cytology ; metabolism ; Porphyromonas endodontalis ; chemistry
9.Efficacy of combined pranoprofen eye drops and artificial tears on the treatment of mild to moderate dry eye syndrome after trabbeculectomy
Guang-Ming, ZHAO ; Ping, ZHAO ; Bao-Ling, NI ; Zhong-Gang, YI ; Cheng, YANG ; Jing-Jing, ZHENG
International Eye Science 2015;(2):370-372
AlM:To evaluate the efficacy of combined pranoprofen eye drops and artificial tears on the treatment of mild to moderate dry eye syndrome after trabbeculectomy.METHODS:This prospective case control study included 63 cases (63 eyes) of patients with mild to moderate dry eye syndrome after trabbeculectomy in our hospital from November 2013 to June 2013. All subjects were randomly divided into two groups. Observation group was treated with combined pranoprofen eye drops and artificial tears and control group received simple artificial tears marking the eyes at 1, 2, 4wk. The patient's symptoms, signs, BUT, S▏t, and FL were observed before treatment and 1, 2, 4wk after treatment.RESULTS: After 2wk, the symptoms of observation group were improved, there was statistically significant difference (P<0. 05). FL difference of each group was statistically significant ( P<0. 05 ); After 4wk, symptoms and signs were improved. There was statistically significant difference ( P < 0. 05 ). The BUT of the observation group and corneal FL scores of two groups showed significant differences (P<0. 05). CONCLUSlON: Artificial tears joint pranoprofen eye drops has good curative effect in the treatment of mild to moderate dry eye syndrome after trabbeculectomy.
10.Congenital malignant melanoma in a neonate.
Hai-ping ZHANG ; Shan ZHONG ; Xian-yi JIANG ; Ming-ni YANG ; Pei-qiong CHEN
Chinese Journal of Pathology 2005;34(1):57-58
Follow-Up Studies
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Forearm
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Humans
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Infant, Newborn
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Male
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Melanoma
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congenital
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surgery
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Skin Neoplasms
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congenital
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surgery