Humans ; Occupational Diseases ; prevention & control ; Occupational Health Services ; statistics & numerical data

Presently,tremendous progress has been achieved in the research of acute pancreatitis (AP),which lead to significant changes in many important aspects of the diagnosis and treatment for AP.In 2014,the Pancreatic Surgery Group of Surgery Branch of Chinese Medical Association revised the Guidelines for the Diagnosis and Treatment of Severe Acute Pancreatitis which was published in 2007.In the revised guidelines,the severity of AP is classified as mild acute pancreatitis (MAP),moderately severe acutepancreatitis (MSAP) and severe acute pancreatitis (SAP).The definition of SAP or MSAP depends on the duration of organ failure,which is persistent (＞48 hours) in SAP but is transient (≤48 hours) in MSAP.Modified CT severity index (MCTSI) is used as the imaging evaluation.Acute peripancreatic fluid collection (APFC),acute necrotic collection (ANC),walled-off necrosis (WON) and pancreatic pseudocyst are the local complications.The disease course is divided into 3 stages,including early stage (acute phase),middle stage (evolution phase) and late stage (infection phase).Infected necrosis is an indication for the surgical treatment,but the surgery should not be performed in sterile necrosis.Meanwhile,surgical treatment should follow the delayed principle.Surgical methods that include percutaneous drainage (PCD),minimally invasive surgery and open surgery could be selected separately orjointly.

Diabetic retinopathy is one of the most common and serious microvascular complications of diabetes, and it is a very complex pathological process, displaying multi-factor, multi-stage characteristic in the outcome. Recent studies have shown that the pathogenesis of diabetic retinopathy is closely related to oxidative stress, and antioxidant therapy can effectively improve the condition. Heme oxygenase-1(HO-1)is a widely existing antioxidant defense enzyme, which can reduce the injury caused by oxidative stress and plays an important physiological role. Studies have indicated that in retina HO-1 can be highly induced by hyperglycemia. Moreover, the expression level of HO-1 by man-made changes can accelerate or postpone the diseases progression. That means HO-1 for the diagnosis and treatment of diabetic retinopathy has satisfactory applied foreground. In this article we would like to review the role of HO-1 in diabetic retinopathy.

Adult ; Dry Eye Syndromes ; Female ; Humans ; Male ; Occupational Diseases ; Welding ; Young Adult

Abscess ; diagnosis ; microbiology ; surgery ; Bacteria, Aerobic ; isolation & purification ; Child, Preschool ; Female ; Fever ; Humans ; Prognosis ; Spleen ; pathology ; surgery ; Splenic Diseases ; diagnosis ; surgery ; Treatment Outcome

ObjectiveTo observe the effect of community-based rehabilitation (CBR) on older stroke patients in ability of activities of daily living (ADL).Methods50 older stroke patients were randomly divided into the rehabilitation group and control group. The rehabilitation group was treated with motor function exercise and ADL training, while the control group only took medicine. Two groups were evaluated with Barthel index before and after treatment. ResultsScores of Barthel index on the rehabilitation group were higher than that on the control group after treatment, and there was a significantly difference between two groups (P<0.01). Conclusions CBR has the significant effect on improving ADL in older stroke patients.

Objective To investigate the effects of Kr üppel-like factor 2 ( KLF2 ) on oxidized low density lipoprotein (ox-LDL) induced expression of microRNA-146a (miR-146a) and proinflammatory cyto-kines (MCP-1 and IL-6) in human umbilical vein endothelial cells (HUVECs).Methods Human umbili-cal vein endothelial cells (HUVECs) were cultured and then stimulated with 50μg/ml ox-LDL for 24 hours. HUVECs were infected with adenovirus vectors over-expressing human KLF2 at an appropriate multiplicity of infection.KLF2-siRNA duplexes were transfected into HUVECs to silence the gene expression .HUVECs were collected at time points of 0 h, 3 h, 6 h, 12 h, 24 h and 48 h after infection.Real-time quantitative-PCR was performed to measure the expression of miR-146a at mRNA level.Silenced endogenous miR-146a using LNA-anti-miR-146 a was transfected into HUVECs with lipofectamine 2000 .The levels of MCP-1 and IL-6 in supernatants were detected by ELISA .Results KLF2 remarkably inhibited the expression of miR-146 a in unstimulated and ox-LDL-stimulated HUVECs in a time-dependent manner .The ox-LDL induced ex-pression of miR-146a, MCP-1 and IL-6 in HUVECs were significantly decreased by KLF2.Silenced expres-sion of miR-146a downr-egulated the ox -LDL induced expression of MCP-1 and IL-6 in HUVECs.Moreover, silenced miR-146 a could partly reverse the inhibitory effects of KLF 2 on ox-LDL induced expression of MCP-1 and IL-6 in HUVECs.Conclusion KLF2 inhibited ox-LDL induced expression of MCP-1 and IL-6 in HUVECs partly through down-regulating the expression of miR-146a.

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Objective To study the expression of acid secreted proteins that are rich in cysteine protein (SPARC protein)in gastric adenocarcinoma tissue and its relationship with the prognosis of patients with gastric cancer.Methods In order to detect SPARC protein expression levels in gastric adenocarcinoma ,104 primary gastric adenocarcinoma samples as well as 30 normal gastric tissues adjacent to carcinoma were studied by immu-nohistochemistry .Results The positive rate of SPARC protein in 30 cases of gastric mucosa adjacent to carcino-ma is 20%,and with weak expression or no expression;The positive rate in 104 cases of gastric cancer tissues was 76.1%,the difference was significant(P<0.01).The high expression rate of SPARC and lymph node metastasis in gastric adenocarcinoma were positively correlated (P<0.01).The SPARC expression rate of well and moderate differentiation of gastric adenocarcinoma were significantly lower than that of the low differentiation ( P<0 .05 ) . The average survival time of gastric cancer patients with high SPARC expression was 27.4 months.However,the average survival time of the patients with low SPARC expression was 40.9 months.The difference was significant (P<0.05).Conclusion SPARC protein is mainly high expressed in gastric cancer stromal tissue cells and fi-broblasts .The expression level of SPARC protein is significantly associated with lymph node metastasis and tumor differentiation degree .The high expression of SPARC patients survival time is short and the prognosis is poor . However ,SPARC protein is not an independent prognostic factor .