2.Clinical Observations on Jin’s Eye Three-needle Acupuncture as Main Therapy for Acquired Paralytic Strabismus
Rong YANG ; Ming CHEN ; Liuzhi ZENG ; Min MAO
Shanghai Journal of Acupuncture and Moxibustion 2015;(10):978-980
ObjectiveTo investigate the clinical efficacy of Jin’s eye three-needle acupuncture as main therapy for acquired paralytic strabismus.MethodSeventy patients with acquired paralytic strabismus were randomly allocated to acupuncture and Western medication groups. Both groups of enrolled patients took prednisone,methycobal, vitamin B1and vitamin B12. In addition, theacupuncture group received Jin’s eye three-needle acupuncture as main therapy. The clinical therapeutic effects and adverse reactions were observed after four and eight weeks of treatment.ResultThe total efficacy rate was 75.0% in the acupuncture group and 51.6% in the Western medication group after four weeks of treatment and 90.6% in the acupuncture group and 77.4% in the Western medication group after eight weeks of treatment. There were statistically significant differences between the two groups (P<0.05). Patients in the acupuncture group occasionally had acupuncture syncope and local blood stasis. Both groups had no special discomforts.ConclusionJin’s eye three-needle acupuncture has a quick and definite therapeutic effect on acquired paralytic strabismus.
3.Multi-slice Spiral CT Manifestations of the Arrhythmogenic Right Ventricular Dysplasia
Lin QI ; Dingbiao MAO ; Ming LI ; Yang CHEN ; Yanqing HUA
Chinese Journal of Medical Imaging 2013;(11):834-836,840
Purpose To investigate the diagnostic value of the arrhythmogenic right ventricular dysplasia (ARVD) using multi-slice spiral CT (MSCT). Materials and Methods Thirty-four patients who were suspected as ARVD received right ventricular radiography, including 16 ARVD patients and 15 non-ARVD patients regarded as control group. The structural and shaped change of heart on reconstructed images of long axis, short axis and four cavity surface and analyze were observed, and MSCT features of right ventricular radiography characteristics were analyzed for ARVD patients. Results Sixteen cases of ARVD were correctly diagnosed by MSCT, and 14 cases had fatty infiltration including 11 cases of apex of heart, 8 cases of inferior wall, 5 cases of anterior wall, 5 cases of anterior wall of funnel area, 3 cases of diagram, 4 cases of papillary muscle, 6 cases of muscular trabecula and moderator band and 1 case of whole right ventricular free wall. Five cases showed scallop sign, 16 cases excessive trabecular change, 11 cases thinned changes, and 16 cases enlarged changes of right ventricular wall. Conclusion MSCT features of right ventricular for ARVD have relative diagnostic characteristics, and the scallop sign and excessive trabecular change of right ventricular wall are its specific imaging characteristics.
4.Intrauterine Growth Retardation Models Caused by Maternal Food Restriction and Its Effect on Important Organs
hui-ming, YANG ; meng, MAO ; su-fei, YANG ; fan, YANG ; tao, YU
Journal of Applied Clinical Pediatrics 2006;0(19):-
Objective To compare the effects of different maternal rats food restriction on newborn rats.Methods Pregnant rats were randomly divided into 4 groups:3 model groups and control group.The model groups were given middling food restriction throughout pregnancy,severe food restriction from pregnant day 14,severe food restriction from pregnant day 7,respectively.Effects of maternal rats food restriction on newborn rats in growth,main organs weight,and small for gestational age(SGA) occurrence were compared.And his-(tiocyte) morphology of cerebra and stomach were observed.Results The weight,height,trail length,and weight of cerebra,heart,lung,(liver),stomach,spleen,and kidney of newborn rats in model groups were significantly different from those in control group(all P
5.Clear cell chondrosarcoma without osteoclast-like giant cells: report of a case.
Rong-jun MAO ; Hui-qiong FANG ; Qi-ming LI ; Ke-fei YANG
Chinese Journal of Pathology 2011;40(6):410-411
Adult
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Chondrosarcoma
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metabolism
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pathology
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Collagen Type II
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metabolism
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Diagnosis, Differential
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Femoral Neoplasms
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metabolism
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pathology
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Giant Cells
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pathology
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Humans
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Male
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Osteoclasts
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pathology
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Osteosarcoma
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metabolism
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pathology
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S100 Proteins
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metabolism
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Vimentin
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metabolism
6.Efects of fennel extracts on gastrointestinal movement of atropine-induced gastrointestinal motility disorder in mice
Guangshou TENG ; Ming QIN ; Fengfeng MAO ; Yan ZHANG ; Xingyou LIU ; Jianrong HE ; Peng YANG ; Manling LIU
International Journal of Traditional Chinese Medicine 2011;33(8):695-697
Objective To observe the effects of fennel essential oil and water extracts (distilled oil is not included) on gastrointestinal motility disorder caused by atropine in mice.Methods Kunming mice were randomly divided into blank control group, model group atropine, water extracts group, fennel essential oil group, mosapride group. Blank control group and model group atropine were orally administered with normal saline of 0.2 ml/10 g. Water extracts group was orally administered with Water extracts (75 mg/ml) of 0.2 ml/10 g. Fennel essential oil group was orally administered with Essential oil of 300 mg/kg. Mosapride group was orally administered with mosapride(15 mg/ml). Subjects were orally treated for 3 d. After fasting for 18 h, blank control group was intraperitoneally injected saline on the fourth day, and other groups were injected atropine sulfate injection to induce animal model of gastrointestinal motility disorder. Blue dextran(BD)2000 was used to observe the gastric emptying rate and rate of intestinal propulsion. Results Gastric emptying rates of fennel essential oil group, mosapride group, water extracts group and model group atropine were respectively(91.97±4.42)%, (90.26±5.81)%, (80.01±6.27)%,(72.88±9.13)%,and intestinal pushing rates were respectively(53.32±7.49)%,(53.02±9.13)%,(44.16±7.68)%,(37.52±6.19)%.Fennel essential oil, mosapride and water extracts enhanced the gastric emptying and intestinal propulsion in gastrointestinal motility disorder animal caused by atropine(P values were 0.004、0.001、0.004、0.003、0.025、0.015),where Fennel essential oil and mosapride were superior to the water,extracts(P values were 0.000、0.002、0.001、0.001).Conclusion Fennel extracts may promote gastrointestinal movement of atropine-induced gastrointestinal motility disorder in mice and fennel essential oil is the main active ingredient.
7.Research progress on stable gene transfection methods of Plasmodium falciparum
LI Xiao-song ; PAN Mao-hua ; HUANG Ya-ming ; YANG Zhao-qing
China Tropical Medicine 2023;23(2):186-
Abstract: Transfection of Plasmodium falciparum is helpful to study the function of its genes, such as drug resistance. However, transgenic manipulation has been very challenging, mainly due to the high A/T base sequence structure (A+T content of about 82%) and low transfection efficiency of the Plasmodium genome. Electroporation-based transfection of Plasmodium falciparum has been successfully applied in the study of certain genes, and electroporation by preloading is currently the preferred method for introducing foreign DNA into Plasmodium falciparum. The site-directed editing of Plasmodium genes mostly adopts the method of two-plasmid transfection. It is generally believed that successful transfection of Plasmodium requires a large amount of high-purity plasmid DNA and an accurate transfection system. In addition to the evaluation of the current commonly used electrotransfection methods, this paper also introduces a new transfection method, namely lyse-reseal erythrocytes for transfection (LyRET). This paper also review the role of factors such as plasmid DNA concentration, the use of transfection reagents, the setting of transfection parameters, the addition of fresh red blood cells, and the markers of successful transfection in improving the success rate and efficiency of Plasmodium transfection, in the hope of providing a reference for study in this field.
8.Design of multi-channel dry type biochemistry sensors based on fiber bundles
Ming YU ; Feng CHEN ; Chao LI ; Biao GU ; Zijian YANG ; Jiawen MAO ; Liangzhe LI ; Taihu WU
Military Medical Sciences 2015;(8):582-586
Objective To develop a multi-channel dry type biochemistry sensor with a compact structure and high measurement accuracy.Methods The principle of double beam compensation based on reference LED was applied to improve the measurement accuracy.The complex splitting system was replaced by MXN fiber bundle and free-form surface lens to make the instrument more compact and lightweight.Use of the adaptive amplification photoelectric detection improved the measurement accuracy while simplifying the process.Results and Conclusion It has been proved by experiments that this sensor has the advantages of high measurement accuracy, little interference and compact construction. This sensor may well meet the requirements of dry type biochemistry analysis.
9.Perspective and application of metabonomics in modern study of traditional Chinese medicine.
Kun-Ming QIN ; Bin WANG ; Lin-Wei CHEN ; Mao-Sen ZHANG ; Guang-Ming YANG ; Ya-Chun SHU ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2014;39(16):3010-3017
Metabonomics is a new method to study on the metabolic network and the relationship between body and environment, which conforms to the way of traditional Chinese medicine (TCM) research. In the study process of modernization of traditional Chinese medicine, effectively conjunction with metabonomics method will facilitate the integration of TCM with modern biological science and technology, and promote the modernization of TCM. This paper introduce the application of metabonomics in the research of toxicity mechanism of TCM, compatibility mechanism of TCM formula, pharmacology effect of TCM and processing mechanism of TCM. This paper summarize the problems in the TCM metabonomics research and prospect its bright future.
Animals
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Drug Therapy
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Drugs, Chinese Herbal
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adverse effects
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analysis
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metabolism
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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methods
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trends
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Metabolomics
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methods
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trends
10.Isoflavonoids from Caragana changduensis and their nitric oxideinhibitory activities.
Xiao-dong SUN ; Shi-ming FANG ; Mao-dan ZANG ; Cheng-xiong YANG ; He-ran LI ; Susumu KITANAKA ; Xue-dong YANG
China Journal of Chinese Materia Medica 2015;40(16):3220-3223
Ten isoflavonoids were isolated from the heartwoods of Caragana changduensis Lion f. by means of various column chromatographic techniques. Based on the detailed spectral data analysis (MS and NMR), as well as comparison with the literatures, their chemical structures were determined as 7,2'-dihydroxy-8,4'-dimethoxyisoflavone (1), 4'-hydroxy-7,3'-dimethoxyisoflavone (2), 5, 7, 4'-trihydroxy-2',5'-dimethoxyisoflavone (3), prunetin (4), afrormosin (5), odoratin (6), genistein (7), texasin (8), pratensein (9), and 6,7,3'-trihydroxy-4'-methoxyisoflavone (10). Among them, compounds 1-3 and 9-10 were isolated from the Caragana genus for the first time. All the compounds were obtained from this species for the first time. In the preliminary assays, compounds 1, 2, 6, and 7 possessed significant inhibitory effects on NO production, with IC50 values of 48.12, 25.32, 62.71, 43.59 μmol x L(-1), respectively.
Animals
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Caragana
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chemistry
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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pharmacology
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Isoflavones
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chemistry
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isolation & purification
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pharmacology
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Macrophages
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drug effects
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metabolism
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Mice
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Molecular Structure
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Nitric Oxide
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antagonists & inhibitors
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metabolism
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RAW 264.7 Cells