OBJECTIVE: To study the effect of rabeprazole on the steady-state pharmacokinetics of clopidogrel in rats. METHODS: Eighteen healthy SD rats were divided into three groups, which were intragastricly administered clopidogrel (30 mg · kg-1 middot; d-1), rabeprazole (8 mg · kg-1 middot; d-1) + clopidogrel (30 mg · kg-1 middot; d-1), omeprazole (8 mg · kg-1middot; d-1) + clopidogrel (30 mg · kg-1 middot; d-1) respectively for 7 d. The plasma concentrations of the metabolite of clopidogrel, SR26334, were determined by HPLC-DAD. The pharmacokinetic parameters of SR26334 were obtained with statistical analysis by DAS 3.0. RESULTS: There was no significant difference between the single-drug group and the rabeprazole combination group in the main pharmacokinetic parameters of SR26334, such as AUC0-t, AUC0-∞, MRT0-t, t1/2, CLz/F, ρmax and ρss (P≤0.05), except that the tmax significantly decreased from (1.17±0.41) h in the single-drug group to (0.58±0.20) h in the combination group (P<0.01). Compared with single-drug group, the AUC0-t, and AUC0-∞ of the omeprazole combination group increased for about 20% (P<0.05), MRT0-t significantly prolonged (P<0.01), and CLz/F decreased for about 20% (P<0.05). CONCLUSION: Long-term combinaton treatment with rabeprazole can accelerate the in vivo metabolism of clopidogrel to SR26334, but there is no significant influence on the degree of metabolism.

Animals ; Apolipoproteins E ; deficiency ; Female ; Hyperlipoproteinemia Type III ; blood ; metabolism ; Lipid Metabolism, Inborn Errors ; blood ; metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Receptors, Calcitriol ; metabolism ; Vitamin D ; blood

Objective To construct the luciferase reporter gene vector of cell division cycle 2 (Cdc2) gene promoter and determine its transcriptional activity. Methods Primers were designed based on human Cdc2 promoter sequence from UCSC software. Then Cdc2 promoter from human genome DNA was replicated. After pGL3-Basic vector and Cdc2 promoter were digested with restriction enzymes SacⅠand XhoⅠseparately, Cdc2 promoter was inserted into pGL3-Basic vector. The recombinant plasmid named pGL3-Cdc2-promoter was transiently co-transfected into U2OS cells with control vector pRL-SV40, and then the activity of dual luciferase was detected. Results pGL3-Cdc2-promoter was constructed successfully. The restriction analysis and sequencing proved the entirely correct sequencing results. The luciferase activity was higher in pGL3-Cdc2-promoter/pRL-SV40 group than that of pGL3-Basic/pRL-SV40 group (1.591 5±0.199 8 vs 0.049 9±0.010 4). Conclusion pGL3-Cdc2-promoter can be transcribed and activated in U2OS cells. This study provided an important basis for screening and evaluation of anticancer drugs.

OBJECTIVE To improve the professional ability of nosocomial infection managers.METHODS By analyzing the status quo and questions among nosocomial infection managers,we explored the essentiality of professionalism construction of the team of nosocomial infection managers and brought forward a way for it.RESULTS It is necessary to set up a professional education and culture system,improve the related guarantee system and keep the team of nosocomial infection managers in stabilization for strengthening the professionalism construction of the team.CONCLUSIONS It is the objective requirement to promote the construction and development of nosocomial infection discipline that impels professionalism construction of the team of nosocomial infection managers.

OBJECTIVE To investigate the function of department of nosocomial infection management in practice.METHODS It was analyzed the situation that the functional management of department of nosocomial infection in China could not be fully used at present.As far as the main content of nosocomial infection control was concerned,it brought forward the idea of performing the functional management of department of nosocomial infection in practice.RESULTS According to establishing the function of department of nosocomial infection management,strengthening the professional ability,and improving the service,both the effect and quality of nosocomial infection management could be enhanced.CONCLUSIONS The same as medical treatment,scientific research,nursing and logistic management,nosocomial infection management should stick to the legal management,and then its function can be effectively used.

Administration, Topical ; Aged ; Aged, 80 and over ; Anthracosis ; complications ; Humans ; Male ; Retrospective Studies ; Thoracic Cavity ; Treatment Outcome ; Tuberculosis, Pleural ; complications ; drug therapy

Adult ; Bone Neoplasms ; pathology ; surgery ; Femur ; pathology ; surgery ; Humans ; Male ; Osteochondroma ; pathology ; surgery ; Young Adult

Animals ; Apoptosis ; Cell Hypoxia ; Cell Proliferation ; Cells, Cultured ; Hydrogen-Ion Concentration ; Muscle, Smooth, Vascular ; cytology ; Myocytes, Smooth Muscle ; cytology ; Oxygen ; metabolism ; Rats

Anti-Bacterial Agents ; therapeutic use ; Antitubercular Agents ; therapeutic use ; Coal Mining ; Drug Therapy, Combination ; Follow-Up Studies ; Humans ; Male ; Ofloxacin ; therapeutic use ; Pneumoconiosis ; complications ; Silicotuberculosis ; drug therapy

Cardiovascular System ; GATA4 Transcription Factor ; metabolism ; Humans