Objective:To summarize the experience of retroperitoneoscopic renal pedicle lymphatic disconnection in the management of chyluria,and to investigate a new minimally invasive treatment of chyluria. Methods:9 cases of chyluria were treated by retroperitoneoscopic renal pedicle lymphatic disconnection from Jan 2005 to Dec 2008 with 7 male ones and 2 female ones. The average age was 48 years. 8 of 9 cases obtained success and 1 of 9 cases was converted to open surgery. Operation time, blooding volume, postoperative complication and hospitalization stay, and the operative effect were observed. Results:The operation time was from 65 to 105 min,with the average of 70 min. The blooding volume was from 50 to 100ml, with the average of 60ml. Chyluria disappeared immediately after operation in all patients. Postoperative stay was from 5 to 7d and no complication happened. Conclusion:Retroperitoneoscopic renal pedicle lymphatic disconnection for chyluria is a effective and efficienl method in the management of chyluria. And it is also safe , minimally invasive , with less blood loss, fewer complications , and short hospital stay .

Objective:To evaluate the application of chromogranin A(CgA) as a marker in the diagnosis of prostate cancer. Methods:Serum CgA and serum prostate-specific antigen (PSA) were detected by immunoradiometric essay in 27 cases of prostatic carcinoma ,27 cases of benign prostate hyperplasia (BPH)and 5 cases of healthy subjects. Results:Serum CgA(98.7?69.42) in patients with prostatic carcinoma was significantly higher than that in healthy subjects(32.68?26.58) and in patient with BPH(44.7?40.86)(P

Entry of enveloped viruses into host cells requires fusion of the viral envelope with a cellular membrane. This step is mediated by viral glycoproteins that undergo a dramatic conformational change. Recent advances in structure and function of the fusion proteins of the class Ⅱ viruses, Rhabdoviruses and Herpesviruses were described. Proteomics computational analyses to locate the functional domain of fusion protein were introduced. The fusion proteins of class Ⅱ and class Ⅰ viruses differ radically in their initial structures but refold toward similar final conformation (trimer of hairpin). The Rhabdoviruses and Herpesviruses have a novel fold combining features of fusion proteins from class Ⅰ and class Ⅱ. The fusion proteins of these viruses have a different conformation change and mediate a different fusion process, therefore, the proteins belong to a novel class of fusion proteins. The potent inhibitor of virus entry should be new strategies for developing antiviral drugs.

Objective To investigate the prevalent characteristics of children with viral pneumonia in different areas and years.Methods The viral antigen of cells of nasopharyngeal secretions was detected by the method of alkaline phosphatasea anti-alkalinephhosphatase in viral pneumonia children in different areas and years.Results Among 627cases , there were 290 positive cases and 14 cases were infected by 2 viral strains. Three hundred and four positive viral strains,71 cases (23.35%) were RS virus infection, 47 cases (15.46%) were AD virus infection, 74 cases (24.34%) were FLU-A virus infection,50 cases (16.44%) were FLU-B virus infection, 47 cases (15.46%) were PIV1,3 virus infection, and 15 cases (4.93%) were PIV2 virus infection. But in different areas, the kind of the viral strain of the highest infection rate was different. The viral detection rate in autumn and winter was the highest and in summer was the lowest. Conclusions FLU-A virus is the main etiological agent of children viral pneumonia,but the main etiological agent in different areas is different. In autumn and winter, the viral infection rate is high. The chance of viral infection of lower respiratory tract of big children is reduced.

Electricity ; Electrosurgery ; instrumentation ; Equipment Design ; Equipment Safety ; methods ; standards ; Guidelines as Topic ; Medical Errors ; prevention & control ; Surgical Equipment ; standards

Humans ; Occupational Diseases ; prevention & control ; Occupational Health Services ; statistics & numerical data

Objective To determine the value of 18F-FDG PET-CT in detecting the primary origin of cancer of unknown primary (CUP).Methods A total of 48 patients (age range 41-82 years,mean age of 57±16 years, 20 females) were retrospectively selected. All patients were diagnosed as CUP and were examined by 18F-FDG PET-CT scan.The patients were divided into cervical lymph node metastasis group (28 cases) and extra-cervical lymph node metastasis group (20 cases) according to different metastasis site.Sensitivity of 18F-FDG PET-CT to detect primary tumor site was compared between these two groups.Results 18F-FDG PET-CT detected primary tumors in 32 of 48 patients (66.7 ％), the cervical lymph node metastasis tumors in 22 of 28 patients (78.6 ％), and extra-cervical lymph node metastasis tumors in 10 of 20 patients (50.0 ％).There was significant difference between cervical lymph node metastasis group and extra-cervical lymph node metastasis group (x2 =4.286,P ＜ 0.05).Conclusion 18F-FDG PET-CT has a high sensitivity to detect primary origin of CUP,especially for patients with cervical lymph node metastasis.

Gastrointestinal stromal tumors (GISTs) is a subset of mesenchymal tumors and represents the most common mesenchymal neoplasm of gastrointestinal tract.In the last decade,GISTs has become well known because of the effectiveness of imatinib mesylate.The molecular targets for tyrosine kinase receptor inhibitor are not only of importance for the treatment of patients but also useful for the development of a novel drug modalities and new strategies in basic cancer therapy.The improved understanding of the molecular mechanism of GISTs has made its diagnosis standardized.Many multicenter phase Ⅱor phase Ⅲ clinical trials have been completed to establish the role of adjuvant and neoadjuvant therapy.It is important to select the correct management strategy,which is multidisciplinary,integrated,and individualized.This paper provides an overview of the rational treatment of GISTs at present.

Diabetic cardiovascular autonomic neuropathy is the most common and troublesome complication of diabetes mellitus.Diabetic cardiovascular autonomic neuropathy contributes greatly to the morbidity,mortality.Factors in the pathogenesis of these complications are altered metabolism,vascular insufficiency,loss of growth factor trophism,and autoimmune destruction of nerves in a visceral and cutaneous distribution.There are studies in progress that suggest cardiovascular autonomic nerves can be induced to regenerate,and the future for patients with diabetic cardiovascular autonomic neuropathy is brighter.

Objective:To study the role of adenoviral-mediated PTEN gene transfection in reversing drug resistance of trastuzumab-resistant human breast cancer.Methods:Recombinant adenoviruses carrying PTEN gene(AdPTEN) were constructed and transfected into trastuzumab-resistant human breast cancer cell line BT474.Proliferation and apoptosis of BT474 cells treated with AdPTEN and trastuzumab were measured by MTT assay and FCM.Time course of BT474 cells apoptosis induced by AdPTEN was analyzed by detection of DNA fragmentation.Western blotting was employed to measure phosphorylated-Akt expression levels in AdPTEN treated BT474 cells.The nude mice model of BT474 cells was employed to observe the effects of AdPTEN and trastuzumab on trastuzumab-resistant human breast cancer in vivo.The expression of PTEN gene,cancer cells apoptosis and ultrastructural changes were evaluated after the mice were sacrificed.Results:We successfully constructed recombinant AdPTEN;and the titer of the recombinant adenovirus was about 4.2?1011TCID50/ml.PTEN gene expression was identified by PCR,RT-PCR and Western blotting assay.Combinatorial AdPTEN and trastuzumab significantly suppressed the proliferation of BT474 cells and induced the apoptosis.The percentage of apopotosis cells treated with AdPTEN was(20.7?5.82)%,companied by cells cycle arrest in G1 phrase(P