Objective:To investigate the expression of Neuropilin-1 (NRP-1) and vascular endothelial growth factor (VEGF) in gastric carcinoma tissue and to examine their relationship with tumor angiogenesis and the biological behavior of gastric carcinoma. Methods: The expressions of NRP-1mRNA and VEGFmRNA were detected by the reverse transcription polymerase chain reaction (RT-PCR) method. Microvascular density (MVD) marked by CD105-positive vascular with SP immunohistochemical staining was also assessed in surgical specimens of gastric carcinoma (GC) and normal gastric tissues (NG). The correlation of NRP-1mRNA, VEGFm-RNA with clinical patholo?gical factors and the relationship among NRP-1mRNA, VEGFmRNA, and MVD were analyzed. Results:The expressions of NRP-1mRNA, VEGFmRNA, and CD105 in GC were significantly higher than those in NG (P<0.01). The expres-sions of NRP-1mRNA and VEGFmRNA were closely correlated with the depth of invasion and lymph node metastasis in GC (P<0.05). A positive correlation was observed between the expressions of NRP-1mRNA and VEGFmRNA (r=0.58, P<0.01). MVD correlated with NRP-1 (r=0.52, P<0.01) and with VEGF (r=0.74, P<0.01). Conclusion:Results indicated that the NRP-1 and VEGF expressions could be involved in the angiogenesis and tumor progression of human gastric carcinoma. The combined detection of NRP-1 and VEGF could also determine the invasion and progression of gastric carcinoma, which plays a particular role in guiding the anti-angiogenic ther-apy of cancer.

By means of the observation of acridine orange-stained lysosomes with fluorescence microscope, we studied the intracellular killing and digestive process after alveolar macrophage (AM) ingested living-yeast. Based on the morphological changes of the cells, we divided the process into three stages: the pre-phagosome-lysosome fusion stage, the phagosome-lysosome fusion and killing stage, and the digestive stage. The results revealed that all the values of phagocytic index, phagocytic rate, fusion index and fusion rate of the isolated AM of rabbits infected with living-BCG were higher than those of normal rabbits (P

Literatures of study on acupoint functional effect of encephalic region with functional magnetic resonance imaging (fMRI) are collected from the database of China National Knowledge Information (CNKI) and Wan fang database. Analysis and comments are made respectively from the status quo of studies on combination of single acupoint, two acupoints and multiple acupoints under both physiological and pathological conditions. The results show that the corresponding encephalic regions can be specifically activated by acupuncture. And different regions can be activated by different acupoints. However, studies on single acupoint, two acupoints and three acupoints are the major researches carried out at present. It is still lack of observation on effect of commonly-used combinations in clinic. Moreover, the reliability of the research results are also affected by disunity of operations protocol, reinforcing or reducing manipulations, intensity or depth of stimulation and duration of needling retention. Therefore, it is necessary to establish a unified and standard operation protocol to strictly control the bias factors which may affect the result of research in the future, and take patients as the study target as much as possible, so as to further explore the central effect mechanism.
Acupuncture Points ; Acupuncture Therapy ; Brain ; diagnostic imaging ; physiology ; Humans ; Magnetic Resonance Imaging ; Radiography

Objective To investigate the specific expression of nucleolin in the development of cap-stage tooth germ of mouse embryo,and detect the possible biological function of nucleolin. Methods In situ hybridization and immunohistochemistry were performed to detect the expression of nucleolin mRNA and protein in the development of tooth germ at embryo day 15(E15).Double staining for apoptotic cells and nucleolin was employed to explore the relationship between nucleoin and apoptosis in tooth germ. Results The expression of nucleolin mRNA and protein was mainly detected in the cervical loop,inner epithelium and the underlying dental papilla.Moreover,nucleolin protein was also located in the primary enamel knot and basement membrane.It was revealed by double staining that the fluorescence images of nucleolin and the location of apoptotic cells were not overlapped. Conclusion The expression pattern of nucleolin mRNA do not completely coincide with that of nucleolin protein at the cap stage of tooth germ.The location for nucleolin protein in the basement membrane suggests that nucleolin may be involved in the reciprocal interactions between the inner epithelium and dental papilla mesenchyme,subsequently affect the morphogenesis of tooth germ.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Combined Modality Therapy ; Female ; Humans ; Middle Aged ; Moxibustion ; Needles ; Postoperative Complications ; etiology ; physiopathology ; therapy ; Scalp ; Treatment Outcome ; Urinary Retention ; etiology ; physiopathology ; therapy ; Urination ; Uterine Cervical Neoplasms ; surgery

CD40 Ligand ; pharmacology ; HL-60 Cells ; drug effects ; Humans ; fas Receptor ; metabolism

Angioplasty ; history ; methods ; Cardiovascular Surgical Procedures ; history ; methods ; Coronary Artery Bypass ; history ; methods ; Coronary Artery Disease ; surgery ; Heart Transplantation ; history ; methods ; History, 20th Century ; History, 21st Century ; Humans ; Stem Cell Transplantation ; history ; methods

Congresses as Topic ; Otolaryngology

Objective:To analyze the metastasis rule of abdominal lymph node from thoracic esophagus carcinoma. Methods:The abdominal lymph node data on 164 patients who had undergone resection of thoracic esophageal carcinoma were analyzed retro-spectively. Grouping was based on the upper, middle, and lower thoracic esophagus. Differences in tumor infiltration depth, differentiat-ed degree, pathological type, pathological stage, and metastasis rate of the abdominal lymph node among the three groups were com-pared. The metastasis rates of the abdominal lymph nodes among the different tumor infiltration depths, differentiated degrees, and path-ological types were also compared. Results:The base condition of tumor infiltration depth, differentiated degree, pathological type, and pathological stage has no statistical significance among the upper, middle, and lower thoracic esophagus. The metastasis rate of the ab-dominal lymph node also has no statistical significance among the three groups (upper, 6.9%;middle, 27.4%;and lower, 39.6%). More-over, the metastasis rate of the abdominal lymph node has no statistical significance among the different tumor infiltration depths, differ-entiated degrees, and pathological types. Conclusion:A special bound lymph node metastasis was present in the esophageal carcinoma. If the tumor in the upper thoracic esophagus infiltrated the submucosa, then it could bound metastasize down to the abdominal lymph node by the lymphatic capillary net. The majority of the esophageal carcinoma was more than T1b period when diagnosed. The tumor has infiltrated the submucosa. Thus, early stage, well-differentiated, and upper thoracic esophageal carcinoma does not indicate mini-mal metastasis of the abdominal lymph node. Routine abdominal lymph node dissection through radical surgery for esophageal carcino-ma was necessary.

Objective To evaluate the second generation micro-endoscopic discectomy (MED) system in the treatment of lumbar disc herniation. Methods Eighty-five patients with single or double level lumbar disc herniation were treated with MED under epidural anesthesia from May 2000 to October 2001.Single disc herniation was in 72 cases, double disc herniation in 13 cases; lateral recess stenosis in 15 cases. Results The average following-up period was 14 months and the successful therapeutic results achieved 92 9%(79/85). the patients were allowed to walk 3 d and discharged 3～5 d after operation. The length of incision was only 1.6 cm. Conclusions MED has short-term satisfactory therapeutic efficacy in the treatment of lumbar disc herniation. The suitable indication and precise technique are the key for the operation.