Dimethyltrilobine iodide (DMT) 1.5 M-M increased the contractile force of isolated guinea pig left atria. This positive inotropic action could not be an-tagonized by either propranolol, cimetidine or diphen-hydramine. DMT 1.5 nM also increased the contractile force of isolated guinea pig left atria exposed to low concentration of calcium or to verapamil.

The pffect of 5 drugs on myocardial nutritional blood flow of micehave been determined with ~(86)Rb. It was shown that the root of xanthiumsibiricam, caesulpinia sepiaria and the branch of cinnamomum cassia couldincrease myocardial nutritional blood flow in mice The Pharmacologicalactions of these drugs have heen reviewed briefly.

Anal Canal ; pathology ; surgery ; Humans ; Laparoscopy ; methods ; Rectal Neoplasms ; pathology ; surgery

Objective:To develop an HPLC method for the content determination of paeoniflorin, tetrahydropalmatine and osthole in Gujinwan capsules. Methods:The determination was performed on an Eclipse XDB-C18 column (250 mm × 4. 6 mm,5 μm) with the mobile phase consisting of 0. 1％ phosphoric acid (adjusting pH to 6. 6 with triethylamine)-acetonitrile with gradient elution. The flow rate was 1. 0 ml·min-1 . The column temperature was 30 ℃. The detection wavelengths were set at 230 nm and 280 nm. Re-sults:The calibration curves were found to be linear within the range of 9. 56-33. 65 μg· ml-1 for paeoniflorin(r=0. 9998), 3. 65-12. 86 μg· ml-1for tetrahydropalmatine(r=0. 9999) and 5. 81-20. 45 μg· ml-1for osthole(r=1. 0000). The average recovery of paeoniflorin, tetrahydropalmatine and osthole was 98. 8％(RSD=1. 1％), 98. 4％(RSD=0. 8％) and 99. 1％(RSD=1. 4％)(n=6),respectively. Conclusion:The method is simple, accurate and reproducible, which can be used for the quality control of Gujinwan capsules.

Thin basement membrane nephropathy(TBMN) is one of the most common disorders of the kidney,affecting at least 1% of the population.It seems to be a disease of the adult glomerular basement membrane(GBM) type Ⅳ collagen trimer ?3∶?4∶?5.Genetic evidence indicates that autosomal TBMN is caused by heterozygous mutations in either COL4A3 or COL4A4, whereas homozygous or combined heterozygous mutations in the same genes lead to autosomal recessive Alport syndrome.The author summarized the epidemiology,clinical features,renal biopsy,genetics,pathogenesis,diagnosis and therapy of TBMN.

Objective:To study the content of polysaccharide and its antitumor activity in the edible part and the disused part of asparagus. Methods:A orthogonal design of 4 factors and 3 levels was applied to optimize the solvent concentration, solid-liquid ratio, extraction duration and temperature that influencing the extraction efficiency. The difference in the polysaccharides content between the two parts was compared, and the difference in the antitumor activity against MCF-7 cells was observed with SPSS software. Results:The optimum extraction technology was used. The difference in the polysaccharide content between the edible part and disused part of asparagus was significant (P<0. 05). The antitumor activity of the two refined polysaccharides against MCF-7 cells was without nota-ble difference (P>0. 05). Conclusion:There is significant difference in the content of polysaccharide in the edible part and the dis-used part of asparagus, however, there is no significant difference in the antitumor activity in the two parts of asparagus.

New investigations indicated that half of the people in the world had the risk of vitamin D deficiency.The main reasons for vitamin D deficiency was that people ignored the importance of using vitamin D supplement in special ages and seasons.Children were potentially at high risk for vitamin D deficiency.Vitamin D deficiency during childhood could cause rickets,growth retardation,skeletal deformities and might increase the risk of osteoporosis and hip fracture in later life.Vitamin D deficiency were also related with cancer,autoimmune disease,endocrine system disease,nervous system disease,hypertension and infection.

Objective: To search for sensitive indicators for the voice assessment of patients with larynx leukoplakia and to analyze the clinical relevance of voice assessment in diagnosis of larynx leukoplakia. Methods: Sixty-three patients with larynx leukoplakia (including 47 males and 16 females) were subjected to voice assessment by Dr. Speech software and the values of Jitter, Shimmer, normalized noise energy (NNE), harmonics-to-noise ratios (HNR), and fundamental frequency (Fo) were calculated. The results of the 47 male leukoplakia patients and 16 females were compared with those of normal controls (male 30, female 30) and of patients with vocal cord polyp (male 30, female 30). Results: The Jitter and Shimmer values of male leukoplakia patients were obviously higher than those of normal controls and patients with vocal cord polyp; their HNR value was lower than those of normal controls and higher than those of patients with vocal cord polyp; their NNE value was lower than those of normal controls and patients with vocal cord polyp (all P<0.05); and their Fo value was similar to those of normal controls and patients with vocal cord polyp. Female leukoplakia patients had a similar result to the male ones. Conclusion: The Jitter, Shimmer, NNE, and HNR values can be used as sensitive indicators for voice assessment of patients with larynx leukoplakia; among them the Jitter and Shimmer values have the strongest sensitivity and can be used as indicators for diagnosis and prognosis of larynx leukoplakia.

Objective To study the glucose-6-phosphate dehydrogenase(G6PD)genotypes in the children of viral hepatitis with G6PD deficiency and investigate the relationship between G6PD deficiency and viral hepatitis.Method DNA samples of 18 children with viral hepatitis/ G6PD deficiency were studied for the 3 common mutations by using the natural primers or mismatched primers mediated PCR followed by restriction enzyme analysis and their clinical manifestation was analyzed.Results Among 18 cases,8 cases were G1388A,and 4 cases were G1376T,and 1 case was A95G.The 3 mutations of G1388A,G1376T and A95G added up to 72.22%.There were no significant difference between the patients with viral hepatitis/ G6PD deficiency and the children only with G6PD deficiency.The incidence rates of acute hemolysis and acute renal failure in the viral hepatitis/G6PD deficiency patients were higher than those in children with G6PD deficiency only.Conclusions It is supposed that the G6PD deficiency is primary in the patients of viral hepatitis with G6PD deficiency.The children of viral hepatitis with G6PD deficiency have more severe pathogenetic conditions and more complications than the children with G6PD deficiency only.