Objective To evaluate the efficacy of repeated application of praziquantel in the treatment of hepatic fibrosis due to schistosomiasis. Methods A total of 60 patients with schistosomiasis hepatic fibrosis(clinically diagnosed cases)were selected and divide into a treatment group and control group randomly,with 30 cases each group. The patients in the treatment group were given praziquantel[30 mg/(kg·d)for 2 days]each year for three consecutive years,on the basis of the conventional liver protection therapy and symptomatic treatment. The patients in the control group were given the conventional liver protection therapy and symptomatic treatment. All the treatment duration was 36 months. The clinical symptoms were observed,and the liv-er function,and the levels of HA,LN,IV-C,and PCIII were detected in the two groups before and after the treatment. Re-sults The clinical symptoms and liver function improved,and the HA,LN,IV-C,PCIII levels were decreased in the treat-ment group with varying degrees,and the total effective rate was 93%(26/28). The total effective rate in the control group was 60%(16/27). There was a significant difference between the two groups in the total effective rate(P<0.05). Conclusion The repeated application of praziquantel has a better therapeutic efficacy in the treatment of hepatic fibrosis due to schistosomiasis.

Adult ; Animals ; Cell Differentiation ; Cell Lineage ; Embryo, Mammalian ; cytology ; Hematopoietic Stem Cells ; cytology ; Humans ; Pluripotent Stem Cells ; cytology ; Regeneration ; Stem Cells ; cytology ; Tissue Engineering ; methods

Objective To explore the molecular mechanism of S100A9-induced secretion of vascular endothelial growth factor-A (VEGF-A)by monocytes.Methods Peripheral blood specimen were collected from healthy individuals undergoing physical exami-nation and the CD14 + monocytes were purified by using immunomagnetic beads and the expression of the receptor for advanced gly-cation endproducts (RAGE)was detected by flow cytomertry.In vitro CD14 + monocytes were stimulated by S100A9,and anti-RAGE antibody or NK-κB signal pathway inhibitor were pre-incubated for 1 hour and then stimulated by S100A9,the levels of VEGF-A were detected by using enzyme-linked immunosorbent assay.Results The high level of RAGE was expressed by isolated CD14 + monocytes,after S100A9 stimulation,the secretion of VEGF-A by CD14 + monocytes was significantly increased in a dose and time dependent manner.However,the inducing VEGF-A was significantly decreased(P <0.01 ),while pre-treated with anti-RAGE antibody or NK-κB inhibitor (P <0.01).Conclusion S100A9 inducing the secretion of VEGF-A by monocytes and is de-pended on RAGE-NK-κB signal pathway,suggesting that S100A9 might promote angiogenesis.

Objective To investigate the vascular architecture of subdermal vascular network in antebrachial region, and provide anatomic basis for designing the subdermal vascular network skin flap in the antebrachial region. Methods Anatomical observation and measurement of the blood supply, arterial architecture and venous return of the subdermal vascular network skin flap in anterior antebrachial region were performed on 8 fresh adult specimens perfused with latex and black ink. Results It was found that 4～15 or more fine perforators derived from each the terminal end of the brachial artery and radial or ulnar arteries in anterior antebrachial region, and their diameters were between 0.2～0.9 mm. The anatomical studies showed that the fasciocutaneous perforators supplying the in anterior antebrachial region were found to form rich "spider nevus"arterial networks on the subdermal layer. Moreover, the subdermal vascular networks were also found to give off fine arterial branches into papillary and superficial fascia layers, respectively, to form papillary vascular plexus and superficial fascia vascular network, and all of the vascular networks were anastomosed densely each other. The subdermal venous networks were also anastomosized in plexus and drained into the deep vein. Conclusion Either a random or an axial subdermal vascular network skin flap of the anterior arm can be designed according to the need of recipient defect, and the latter may also be designed as a transfer or a free subdermal vascular network skin flap according to the size of the arterial diameter in the pedicle of skin flap.

Objective To compare the therapeutic efficacy between laparoscopic peritoneal vaginoplasty and sigmoid loop vaginoplasty. Methods A total of 29 cases of congenital absence of vagina received either laparoscopic peritoneal vaginoplasty (Peritoneal Group, 14 cases) or sigmoid loop vaginoplasty (Sigmoid Group, 15 cases) from March 1998 to April 2003. Results The operation failed in 1 case in the Peritoneal Group, requiring a conversion to open sigmoid loop vaginoplasty. Both groups had no surgical complications or postoperative pyrexia. The operating time of the Peritoneal Group (118 9?19 0 min) was significantly shorter than that of the Sigmoid Group (202 0?18 6 min) ( t =-11 674, P =0 000). And the intraoperative blood loss of the former (36 2?12 6 ml) was significantly less than that of the latter (105 3?46 3 ml) ( t =-5 205, P =0 000). The conditions of reconstructed vagina of both groups were basically the same, but the vagina length of the Sigmoid Group (10 8?1 1 cm) was significantly greater than that of the Peritoneal Group (9 0?0 8 cm) ( t =-4 882, P =0 000). Among the Sigmoid Group, 3 cases had somewhat more amount of vaginal secretion and 4 cases had abnormal-smelling secretion. Patients in both groups reported the same satisfactory degree of sexual life. Conclusions Laparoscopic peritoneal vaginoplasty and sigmoid loop vaginoplasty present a similar therapeutic efficacy, but the former gives less surgical invasion than the latter.

Objective To study the relation between the clinical feature and glycoprotein D gene sequence analysis of a wild strain of HSV 2 isolated from one relapsed patient with genital herpes. Methods The partial glycoprotein D gene sequence of the above mentioned strain was amplified and cloned with PCR. Results The comparison of the amino acid sequence of gD gene between the wild strain and HSV 2G strain published showed that there was a mutation at site80 and site159. Conclusion In order to develop effective vaccine which is suitable for China, it is necessary to investigate the differences of the gene structure and function of gD among HSV isolated from China and other countries.

Objective To investigate if corticosteroid could influence periphe ral blood CD3＋、 CD4＋ and CD8＋ cells in secondary syphilis and the prognosis of syphilis. Methods CD3＋、 CD4＋、 CD8＋ cells were detected in 11 patients wh o took corticosteroid and 20 patients who did not. The effects on the therapeuti c effect of lesions were observed. Results The levels of CD3＋ and CD4＋ in peri pheral blood were significantly lower in corticosteroid group than those in cont rols. The proportion of patients whose RPR test turned to negative and lesions c ured was significantly lower in corticosteroid group than those in controls with in the first three months after treatment. Conclusion Corticosteroid administrat ion decreases the levels of CD3＋ and CD4＋ in secondary syphilis and therefore influence the prognosis of syphilis.

Objective: To investigate the growth inhibition effect of caffeic acid and DDP (cisplatin) on human lung adenocarcinoma A549 cells in vitro, and to explore the possible mechanism. Methods: A549 cells were cultured in vitro. The effect of caffeic acid alone or in combination with DDP on proliferation of A549 cells was detected by MTT assay. The effect of caffeic acid alone or in combination with DDP on apoptosis of A549 cells was detected by Hoechst33258 immunofluorescence staining and flow cytometry. The expressions of Bcl-2, Bax and caspase-3 proteins were detected by Western blotting. Results: Caffeic acid or DDP alone or in combination could significantly inhibit the proliferation and induce apoptosis of A549 cells, and the combination of these two drugs had a synergistic effect. Western blotting result showed that the combination of caffeic acid and DDP synergistically inhibited the expression of Bcl-2 protein and enhanced the expression of Bax protein and the activation of caspase-3. Conclusion: Both caffeic acid and cisplatin may improve the growth-inhibitory and apoptotic activities of human lung cancer A549 cells in vitro. These effects are enhanced with the combination of caffeic acid and DDP in a synergistic manner. Copyright © 2012 by TUMOR.

Objective To evaluate the efficacy of uterosacral ligament amputation in combination with lesion electrofulguration under laparoscope in the treatment of endometriosis-related dysmenorrhea. Methods A total of 86 patients with endometriosis-related dysmenorrhea were treated by uterosacral ligament amputation combined with electrofulguration of lesions under laparoscope from March 2000 to August 2004 in this hospital. Among the 86 patients, enucleation of ovarian endometriotic cysts and electrofulguration of pelvic endometriotic lesions were also simultaneously conducted in 37 patients, and electrofulguration of all visible pelvic endometriotic lesions was also performed in the rest of 49 patients. The operation was accomplished with the aid of the placement of an infrared ureteral detector in 3 patients because of the thickening of the uterosacral ligament and local adhesion. Results The 86 patients were followed for 6~48 months (median, 23 months). Dysmenorrheal completely disappeared in 36 patients, partially subsided in 29, and remained unchanged in 21, the effective rate being 75.6% (65/86). Conclusions Uterosacral ligament amputation combined with electrofulguration of lesions under laparoscope is effective for the treatment of endometriosis-related dysmenorrhea.

Pseudorabies is an important infectious disease for many kinds of livestock and wild animals, and causes important economics losses for pig industry. Many kinds of vaccines including attenuated live viruses or inactivated are widely used for vaccination of pigs and other animals. In the present review, research advances on pseudorabies new-type vaccines such as subunit vaccine, DNA vaccine, recombination vaccine, deletion-mutant vaccine is presented and point out the further development of the vaccine.