Analyses the results of 14 kinds of drugs in mutational tests by grey cluster analysis were reported. A more rational mathematical model for quantitative evaluating the results was established. According to the value of grey relationship grades, this drugs were classified into 4 groups by "Net-making" cluster: strong mutagen, medium mutagen, weak mutagen and non-mutagen. The mode} could be calculated simply and its calculating results had a high correspondency with special qualitative analysis. In addition the function of CHL test, MN test and Ames test were evaluated by grey superiority analysis.

Flail chest is the common chest trauma, which causes abnormal respiration and mediastinal swing affecting the respiratory and circulatory function after injury. Fixation of ribs of flail chest has been used for half a century, methods and equipment of which varied, and domestic and foreign scholars have verified its efficacy in clinical practice. This article summarizes its main great progresses.

Objective To explore the mechanism of Shuilu erxiandan against male′s infertility and asthenoospermia .Methods Human sperm with normal physiological function were collected ,and then sperm suspension were divided into the normal group ,the model group ,Vitamin C group and the groups of large ,medium and small dose of Shuilu erxiandan .The reactive of species was made from hypoxanthine-xanthine oxidase .In the oxygen environment ,Vitamin C(0 .25 g/L) and different content (1 .0 ,0 .5 ,0 .25 g/mL) of extract from Shuilu erxiandan were hatched with sperm .Then the function of sperm membrane in every group was evalu-ated using hypoosmoticswelling .Meanwhile the content of MDA and SOD were detected .Results Expansion of sperm and sperm motility in all the groups of vitamin C and large ,medium and small dose of Shuilu erxiandan′s extract were significantly higher than that in the model group at time point of 1 ,3 and 6 h (P< 0 .05) .Also ,medium dose of Shuilu erxiandan (0 .5 g/mL) displayed more effective on expansion of sperm and sperm motility compared with that in the vitamin group C (P< 0 .05) .All the groups of vitamin C and large ,medium and small dose of Shuilu erxiandan′s extract significantly indicated descreased MDA content and improved SOD activity compared with that in the model group (P< 0 .05) .Also ,this effect of medium dose of Shuilu erxiandan (0 .5 g/mL ) on MDA and SOD activity were superior to that in vitamin C group ,which showed significant difference (P< 0 .05) .Conclusion Ex-tract from Shuilu erxiandan could significantly protect structure and function of sperm membrane ,which is correlated with interve-ning its lipin peroxidation .

Objective To investigate therapeutic effect,safety and post transplant immunological reconstitution for patients of lymphoma after autologous bone marrow transplantation and occurrence of Rheumatoid Arthritis.Methods The clinical situation,laboratory index and immunological reconstitution was observed for patients of lymphoma after inductive treatment,BCEA of pretreatment,autologous bone marrow transfusion,G-CSF and radiotherapy to lymph node.Results Recovery of hemopoiesis was observed after 28 days.Disappearance of joint swelling and negative ESR,CRP,IgMRF were observed after 2 months.The patients started to work after 3 months. No recurrence of lymphoma and Rheumatoid Arthritis has been observed until today.Conclusion The autologous bone marrow transplantation was safe and effective for the treatment to patient of lymphoma with rheumatoid arthritis,which was a new method for the treatment to lymphoma and other autoallergic diseases.

10.3969/j.issn.2095-4344.2013.25.018

Objective:To explore the influence of diabetic self-management education on blood glucose control and inflammatory factor levels in inpatients with diabetes mellitus (DM).Methods:A total of 168 DM patients,who hospitalized in department of endocrinology in 163rd hospital of Chinese PLA from Jun 2012 to Jun 2013,were ran-domly divided into self-management education group (self-management group,n=88)and routine health education group (routine management group,n = 80).Self-management group received self-management education course namely AADE7TM ,provided by full-time DM health education staff,including healthy eating,being active,monito-ring,taking medication,problem solving,healthy coping and reducing risks,while routine management group re-ceived routine DM health education.Blood glucose control,changes of levels of myeloperoxidase (MPO),tumor necrosis factor-α(TNF-α)and intercellular adhesion molecule-1 (ICAM-1)were examined before and after interven-tion.Results:Compared with before intervention,there were significant reductions in levels of fasting blood glucose (FBG),postprandial blood glucose (PBG)and glycosylated hemoglobin (HbA1c)in both groups after interventions (P <0.05 all).Compared with routine management group,there were significant reductions in levels of FBG [(7.1 ±1.3)mmol/L vs.(6.0±1.1)mmol/L],PBG [(9.5±1.6)mmol/L vs.(8.8±1.4)mmol/L]and HbA1c [(7.9 ±1.5)% vs.(7.2±1.1)%]in self-management group,P <0.05 all.Before discharge,there were significant de-crease in inflammatory factor levels in both groups compared with before intervention (P < 0.05 all).Compared with routine management group after intervention,there were significant decrease in inflammatory factor levels [TNF-α:(201.0±57.3)pg/ml vs.(100.7±49.2)pg/ml,ICAM-1:(25.2±3.6)ng/ml vs.(18.2±2.9)ng/ml] in self-management group,P < 0.05 both.Conclusion:Self-management education provided by full-time diabetic health education staff is superior to routine diabetic health education method in controlling blood glucose and decrea-sing inflammatory factor levels,indicating it can improveβcell function and relieve insulin resistance.

Pathogenic diagnosis is very important for the management of sellar mass.Many reasons can cause sellar mass,which makes it easily to be misdiagnosed and subsequently lead to mismanagement.The aim of this article is aimed to catch physicians′ attention to the differential diagnosis of sellar mass,in order to raise the management level of sellar mass and to reduce its misdiagnosis and mismanagement.

Objective To investigate the clinical characteristic and risk factors of corticosteriod-induced ocular hypertension after vitreoretinal surgery.Methods Retrospective analysis was made based on the clinical data of 866 patients,including 126 corticosteriod-induced ocular hypertension(89 male,37 female,age range 9-70 years old) after vitreoretinal surgery during May 2002 to May 2005.Results All cases used glucocorticoid locally or systemically.Multi-variance logistic analysis showed that male,complicated vitreoretinal surgery,longer hospital stay,therapeutic duration with glucocorticoid,cataracta were the independent predictive factors,while myopia,hypertension,diabetes were not closely related to corticosteroid-induced ocular hypertension.Conclusion The incidence of corticosteriod-induced ocular hypertension after vitreoretinal surgery was 14.55%.The important factor leading to ocular hypertension is long-term application of glucocorticoid.

Objective To explore the relationship between sperm quality and neurtophil elastase (NE) in male infertile patients . Methods the NE concentration was detected in1 30 male infertile patients ,the research subjects were classified into 3 groups based on the neurtophil elastase concentration :neurtophil elastase concentration more than 1 000 ng/mL (group A ) ,250-1 000 ng/mL (group B) less than 250 ng/mL (group C) .Then sperm basic parameters and biochemical markers (phosphatase ,citrate ,zinc and fructose) were detected .Results (1) The sperm density and motility showed statistically significant differences among the group A ,B and C(P<0 .05)moreover neutrophil elastase concentration was negatively correlated with sperm density (r= -0 .43) and sperm motility(r= -0 .37) .Sperm volume and morphology showed no statistically significant differences among three groups (P>0 .05) .(2) There were statistically significant differences in the levels of phosphatase ,citrate and zinc among three groups (P<0 .05) ,moreover the neutrophil elastase concentration had significantly negative correlation with the concentrations of phosphatase , citrate and zinc(r= -0 .19 ,r= -0 .17 ,r= -0 .23) ,while the fructose concentration had no statistical difference among three groups(P>0 .05) .Conclusion The NE level in semen has significant reverse correlation with sperm density ,sperm motility and bi‐ochemical markers (phosphatase ,citrate and zinc) ,implying that semen NE has significantly negative effect on the sperm quality , thus affecting male fertility ability .

Objective Clinical research was made to observe the relationship between idiopathic thrombocytopenic purpura(ITP)and Helicobacter pylori(Hp)and to evaluate the effect of Hp eradication in the treatment of refractory ITP.Methods 13C urea breath test and Hp antibody of serum were made in 48 previously untreated patients with ITP from Department of Hematology of the First Affiliated Hospital of China Medical University during November 2004 to May 2005 years and normal controls consisted of 52 heathy peoples whose gastroscopy was normal.Omeprazole,clarithromycin and amoxicillin were used for Hp eradication to refractory ITP with Hp infection.13C urea breath test,Hp antibody of serum and the level platelet and PAIgG was detected after therapy 4~8 weeks.Results Of 48 cases with ITP,33(68.18%)had Hp infection and 24 of 52(46.12%)normal population had Hp infection,the infection rate of Hp in ITP patients being statistically higher than the control group(P