The pffect of 5 drugs on myocardial nutritional blood flow of micehave been determined with ~(86)Rb. It was shown that the root of xanthiumsibiricam, caesulpinia sepiaria and the branch of cinnamomum cassia couldincrease myocardial nutritional blood flow in mice The Pharmacologicalactions of these drugs have heen reviewed briefly.

Acupuncture Therapy ; Acupuncture, Ear ; Adolescent ; Adult ; Child ; Conjunctivitis ; therapy ; Female ; Humans ; Male ; Young Adult

Objective To summarize treatment experiences of microsurgical clipping for anterior communicating artery aneurysm via pterional approach.Methods Clinical data of 82 cases undergoing microsurgical clipping for anterior communicating artery aneurysm via pterional approach in the first affiliated hospital of Kunming Medical University from October 2008 to December 2014 were collected and retrospectively analyzed.The patients were divided into different groups by Hunt-Hess illness grading scale,with 11 cases for level 0,7 cases for level Ⅰ,30 cases for level Ⅱ,25 cases for level Ⅲ,8 cases for level Ⅳ,and 1 cases for level Ⅴ.Twenty-one patients underwent operation at early stage of SAH (＜3d),15 at late stage of SAH (4d～2W),and 35 at prolonged stage of SAH (＞2W).The prognosis of patients was evaluated according to GOS classification criteria at discharge.Results A total of 85 ACoAA were found in 82 patients and all of them were clipped and,at the same time,3 aneurysms were resected and 11 thrombuses were punctured,cut and removed.In the operation,15 (17.6％) aneurysms ruptured again and temporary blocking happened for 73 times,with the shortest blocking time of 2 rmin,the longest of 40 rmin,and the average of 9.26min.According to GOS score,good recovery rate was 79.3％ (65/82),moderate disability rate was 12.2％ (10/82),severe disability rate was 3.7％ (3/82),vegetative state rate was 0％,and death rate was 4.9％ (4/82).Good recovery rates for the operations at early,late and prolonged stage of SAH were 85.7％,73.3％ and 82.3％ respectively and for level 0 to level Ⅴ were 90.9％,85.6％,86.7％ and 84.0％,25.0％ and 0.0％ respectively.Results of DSA or CTA re-examination upon 55 patients followed-up at discharge or three months after discharge showed that tumor pedicle were clipped and aneurysm disappeared.Fifty cases were followed up from four months to seven years after the operation,with one case of aneurysm recurrence.Another thirty-two cases were lost to follow-up.ConclusiornS Microsurgical techniques and microdissection are keys to successful operation.Pterional approach can guarantee successful clipping of aneurysms with different directions,locations and sizes.It is also an easy,effective and reliable approach with less postoperative complications.Operation for anterior communicating artery aneurysm should be performed as early as possible.

Objective To determine whether vascular endothelial growth factor (VEGF) could be used as a tumor marker by detecting the VEGF levels in serum and effusion from malignant tumor patients. Methods VEGF concentrations were measured using an enzyme-linked immunosorbent assay in the serum from the healthy donors and in the serum and the malignant effusion from the patients. Results The serum VEGF levels from the malignant tumor patients was higher than that from the healthy donors, and there was a significant difference ( P

Objective To find out if different doses of melatonin have different roles in the cause or treatment of idiopathic scoliosis, and to illuminate whether melatonin can be a substitution in the treatment of pinealectomized chickens. Methods Fifty Little-Shaoxing chickens, in which pinealectomy was performed when 3-day-old, were divided into five groups. Ten served as control group, were kept in light-dark (12h: 12h) cycle, 500 lx in daytime and 0-5 lx in nighttime after surgery. Ten served as M-5 group, were given 5 mg/kg (5 milligrams per kilogram of body weight) melatonin twice every day. Ten as M-10 group, given 10 mg/kg melatonin abdominally twice every day, ten as M-20 group, given 20 mg/kg melatonin twice every day, and ten as M-40 group, given 40 mg/kg melatonin twice every day. The light-dark (12h: 12h) cycle was the same as control group. Radiological examinations were performed on all chicken spines for scoliosis when at 3 months old. Measured of the angle of the scoliosis was taken by Cobb's technique, and the data was analyzed by statistical software. Results Overall, there was 25 chickens developed to scoliosis in all when the chickens were 3 months. The attack rate was only 56%; this rate was consistent throughout all experimental groups. In the control group, 6 chickens had obvious scoliosis, and 5 in M-5 group, 5 in M-10 group, 4 in M-20 group, and 5 in M-40 Group. And the average Cobb's angle of control group was 26.7?. There were 23.5?, 21.7?, 24.5? and 23.2? in M-5, M-10, M-20 and M-40 respectively. There was no statistical significance in the average Cobb's angle and the incidence of scoliosis among all groups. It was determined that neither the prevalence nor the pattern of the scoliosis was affected by the therapy in any of the experimental groups. Conclusion Different dose of melatonin can't reduce the rate of scoliosis in the pinealectomized chickens, and also can't prevent the development of the experimental scoliosis. The results of this study raise doubts regarding the role of melatonin in the development of scoliosis after pinealectomy in the young chicken. Melatonin acting as a vicarious therapeutic tool in the treatment of idiopathic scoliosis is premature, deserved further research.

OBJECTIVE:To prepare Capsaicin liposome and to investigate its stability.METHODS:0.075%capsaicin li-posome(W/V)was prepared with thin-film dispersion and micro-fluidic technology.The stability indices such as grain size,surface potential and encapsulation efficiency were investigated.RESULTS:The prepared liposome was characterized by small and steady grain size.No obvious changes were found in appearance,grain size,surface potential and encapsulation efficiency of liposome under the airtight storage for9days or stored at4℃for10months.CONCLUSIONS:The technology of liposome can encapsulate capsaicin,and maintain good stability in the short period of time.

[Objective] To introduce a method of arthroscopic synovectomy in chronic synovitis of the knee in patients with hemophilia A and to evaluate its clinical value.[Method]Arthroscopic synovectomy was performed in 25 patients with hemophilia A from Sept.2002 to Jan.2007.Sufficient factor replacement was used and systematic rehabilitation exercise was emphasized.HSS scores were used to evaluate the efficiency of the operation.Insall standard was used to determine the difference between groups according to Outerbridge grading of the cartilage.[Result]Twenty-three patients were followed up.The mean period of follow-up was 27 months.The average frequency of hemarthrosis reduced from 23.4 times per year preoperatively to 9.8 times per year postoperatively(P

[Objective]To compare the changes in femoral bone tunnel after arthroscopically assisted hamstring ACL reconstruction with different graft fixations and investigate the relation with clinical effect.[Method]Eighty-eight cases in three groups were included and underwent hamstring ACL reconstruction with different femoral graft fixations for average 16.5 months follow-up evaluation.Twenty-six cases in group A received Endobutton fixation,twenty-two cases(group B) received interference screw,and forty cases(group C)received Rigidfix system.Femoral bone tunnel diameters at the articular line level,the widest point,1 cm away from tunnel aperture on sagittal MRI were compared at one week postoperatively.Lysholm score was used to evaluate the clinical effect.[Result]Tunnel enlargement after ACL surgery was noted in the three groups.At the femoral widest point and 1 cm away from tunnel aperture,the tunnel enlargement was larger in group B and smaller in group C.At the femoral articular line level,the bone tunnel enlargement in group C was smallest.There was no difference between those of groups A and B.The shape of femoral tunnel was lineal in groups A and C,and conical in group B.No significant difference was found by Lysholm score.[Conclusion]The type of graft fixation is a major factor in the development of tunnel enlargement after ACL surgery.No correlation between graft fixation,bone tunnel enlargement and clinical effect has been found.

Objective To study the expression and significance of indoleamine-2,3-dioxygenase(IDO)in non-small-cell lung cancer(NSCLC).Methods Thirty-eight cases of lung cancer tissues and 10 cases of paracancerous normal lung tissue were collected.The semiquantitative immunohistochemistry was used to detect the positive rate of IDO and fluorescent quantitative PCR to detect the level of IDO mRNA in the lung tissues respectively.Results The positive rate of IDO and the level of IDO mRNA in NSCLC group were significantly higher than in paracancerous normal lung tissues(P＜0.05).There was no significant difference in the positive rate of IDO and the level of IDO mRNA between the adenocarcinoma group and the squamous cell carcinoma group(P＞0.05).Statistically,there was significant difference in positive rate of IDO and the level of IDO mRNA among low,middle and high differentiation groups(P＜0.05).There was significant difference in positive rate of IDO and the level of IDO mRNA between the lymph node metastasis and non-metastasis groups(P＜0.05).Conclusion The high expression of IDO in NSCLC is correlated with the degree of differentiation and the metastasis of lymph nodes,but not with the pathological types.

In order to investigate the effect of Polo-like kinase-1 (Plk1) depletion on cell cycle progression and cell growth in lung cancer cells, a recombinant plasmid containing antisense RNA targeting Plk1 (pcDNA3-Plk1) was transfected into A549 cells by lipofectine. RT-PCR and Western-blot were used to detect the Plk1 gene expression. Cell proliferation was evaluated by direct cell counting and bromodeoxyuridine (BrdU) labeling. Cell cycle distribution and apoptosis were examined by flow cytometry, and the inhibition rate (IR) by vinorebline (NVB) was determined by MTT assay. The results showed that after transfection of pcDNA3-Plk1 into A549 cells, the expression levels of Plk1 mRNA and protein were greatly decreased. In pcDNA3-Plk1 transfected groups, abnormal morphological changes of cells and growth inhibition were observed, and the BrdU labeling index was significantly lower than in the control groups (P<0.05). Cells in pcDNA3-Plk1 transfected groups were arresed in G2/M phase and apoptosis was detectable 72 h post transfection. IR induced by vinorebline in pcDNA3-Plk1 transfected groups was significantly higher than in other groups. These data suggested that antisense RNA targeting Plk1 could suppress the Plk1 expression, and therefore, significantly inhibit cell proliferation and induce cell cycle arrest and apoptosis. Moreover, it sensitized lung cancer cells to chemotherapy.