Objective To explore the diagnostic value of Reid Scores under colposcopy in cervical human papillomavirus (HPV)infection. Methods Cervical biopsy under colposcopy was carried out for cervical diseases in 528 patients.With pathological findings as criteria,the value of Reid Scores under colposcopy in diagnosis of HPV infection was analysed.Results The coincidence of Reid Scores under colposcopy in diagnosis of HPV infection and cervical intra-epithelial neoplasia(CIN)was 52.66%,with the sensitivity of 51.05%in diagnosis of low-grade cervical diseases(HPV infection and CIN I)and that of 66.67%in diagnosis of high-grade cervical diseases(CIN Ⅱ and CIN Ⅲ).For the patients with diagnosis of HPV infection examined by pathology,the scores of items of boundary,color and vessels were positively related to the total Reid Score,while the scores of items of boundary and color were not related to that of vessels. Conclusion There may exist clinical significance in diagnosis of HPV infection by colposcopy in combination with RCI Scores.

Objective To establish the method of content determination of icariin in Jiawei Wuzi Yanzong Pill. Method The HPLC method was used to determine the content of icariin from Jiawei Wuzi Yanzong Pill. The analysis was carried out on Lichrospher C18 column (250 mm?4.6 mm, 5 ?m) with acetonitrile-water (30∶70) as mobile phase, flow-rate of 1.0 mL/min and wave-length at 270 nm at 30 ℃. Results The liner range of icariin was 0.28～2.60 ?g with a correlation(r) of 0.999 9. The recovery was 100.86% with RSD of 0.71%. Conclusion The method had good reproducibility and sensitivity to determine the content of icariin from Jiawei Wuzi Yanzong Pill.

Objective:Combined laparoscopy and hysteroscopy in the diagnosis and therapy of the female infertity is and advanced means in the gynecologcial field.We analysed the causes and the treatments of the 71 infertile patients.Pelvic adhesions,endometrosis and salpingtis were the main causes of infertity in our 71 cases.Of 71 cases,29 cases were primary infertity,in whichc endometriosis,PCOS and anatomic uterine defect were the main causes,and 42 cases were secondary infertity,in which 35 cases (83%) had previous intrauterine operations.All the patients were treated for the causes.We concluded that combined laparoscopy and hysteroscopy can diagnose the causes of infertity and can observe the tubal patency by hysteroscopic tubal cathertetization and hydrotubation,which will direct the treatments and estimate the results of the therapy.Methods:Infertity;Combined Laparoscopy and Hysteroscopy.

Objective To analyze the features of menopausal syndrome and its risk factors in patients from menopausal outpatient clinic,and further to evaluate menopausal syndrome and standardization managenent pathway of treatment.Methods The objects were enrolled in the menopausal outpatient clinic from Apr.2010 to May.2015,completing the self-designed questionnaires through one-by-one interview,including demographic information,and modified Kupperman Index.Results The average age of 907 menopausal women was 51.74+4.94 (aging from 30 to 70 years old).The incidence of (peri-)menopause syndrome was 95.7％,increasing with the prolonging of menopausal duration.The incidence and severity of surgery-related menopausal syndrome were higher than those of natural menopause.The five most frequent symptoms were:fatigue (84.6％) ＞ insomnia (77.3％) ＞ hot flashes sweating (76.3％) ＞ mood swings (72.3％) ＞ joint pains and heart palpitations (72.1％).Other factors such asage,menopausal status etc.also affected the menopausal symptoms.Conclusions Prevalence of (peri-) menopausal syndromes was high and it has many influencing factors.The incidence and severity of surgery-related menopausal syndrome are higher than those of natural menopause.

BACKGROUND: Nanohydroxyapatite reinforced polyamide 66 (n-HA/PA66) prepared using the novel process based on nanohydroxyapatite (n-HA) sol may promote the biocompatibility due to the well distribution of n-HA in the polyamide66 (PA66) matrix and chemical bond at organic-inorganic interface, n-HA/PA66 composite has been proposed as a premising bone repair biomaterial.OBJECTIVE: To investigate the biocompatibility of the novel material both in vitro and in vivo.METHODS: Primarily cultured osteoblasts were co-cultured with n-HA/PA66 and PA66. The cell attachment and morphology were studied using phase contrast light microscope (PCLM) and field-emission scanning electron microscope (FE-SEM).Moreover, n-HA/PA66 pins, with PA66 pins as control, were implanted into the right and left (control group) shinbone shafts of the rabbits, respectively. Materials were harvested at weeks 2, 8 and tissue sections were observed.RESULTS AND CONCLUSION- n-HA/PA66 and PA66 exhibited no cytotoxicity with osteoblasts, while the cellular morphology near the samples was better in the test group than in the control one. Moreover, the cell density attached on n-HA/PA66 was higher than that on PA66 and the cell number were especially different after co-culturing for 3 days (P < 0.01 ). The osteoblasts at the interface between the host bone and n-HA/PA66 also had a higher activity during the earlier period after implantation.Meanwhile the bone formation process was faster and effective in the experimental group. Results suggested that n-HA/PA66 prepared base on the n-HA sol has a better biocompatibility as compared with PA66.

Objective To investigate the expression pattern and significance of two importantoocyte-secreted factors:growth differentiation factor 9(GDF9)and bone morphogenetic protein 15(BMP15)during oocyte maturation in women with polycystic ovary syndrome(PCOS)and infertile women due to husband factors.Methods Total of 25 oocytes[9 at germinal vesicle GV stage,9 at M Ⅰ stage and 7 at M Ⅱ stage]were obtained from 12 patients with PCOS and 82 oocytes(29 at GV stage,26 at M Ⅰ stage and 27 at M Ⅱ stage)were from 56 controls.The nested quantitative real time(RT)PCR was uscd to detect the abundance of GDF9 and BMP15 mRNA in each oocyte.Results(1)The expression level of GDF9 mRNA at the GV stage,M Ⅰ stage and M Ⅱ stage in PCOS group were 44.8(4.2-529.0),27.6(9.8-172.7)and 49.0(0.2-65.9)respectively,the expression in were 149.9(55.4-387.9),29.9(2.5-205.8)and 657.8(149.4-1376.2)in control group,respectively.The expression of GDF9 mRNA at M Ⅱ stage was significantly lower in PCOS group than in controls(P ＜ 0.01),however,the differences didn't reach statistical significant at GV or M Ⅰ stage between the two groups(P ＞ 0.05).The expression of GDF9 mRNA displayed some changes at different maturation stage in controls(P ＜ 0.05,P ＜ 0.01),however,the expression didn't demonstrate any dynamic changes in PCOS group(P ＞ 0.05).(2)The expression level BMP15 mRNA at the GV stage,M Ⅰ stage and M Ⅱ stage in PCOS group were 0.1(0.1-22.0),3.2(0.6-55.0)and 6.4(3.2-8.5),respectively,the expression were 41.6(6.5-96.1),4.0(2.0-10.4)and 49.7(2.3-139.5)in control group,respectively.The expression of BMP15 mRNA at GV stage was significantly lower in PCOS group than in controls(P ＜ 0.01),however,the differences were not significant at M Ⅰ or M Ⅱ stage between the two groups(P ＞0.05).The expression of BMP15 mRNA also displayed some changes at different maturation stage in controls(P ＜ 0.05),however,the level didn't demonstrate any dynamic changes in PCOS group(P ＞ 0.05).Conclusion It was suggested that the low expression of oocyte secreted factors in mature oocytes from PCOS patients might be associated with impaired oocyte quality and developmental competence in PCOS.

Objective To investigate condition of single drug or multiple drug resistance and sensitivities of different combinations of antibiotics in Helicobacter pylori (H.pylori) infected patients of different ages in Jiaxing City,Zhejiang Province.Methods From January 2007 to December 2011,a total of 6280 patients underwent gastroendoscopy examination were enrolled,the average age of them was 50.8 years old.Among them,129 cases were less than 20 years old,1802 cases were between 20 and 40,3016 cases were between 40 and 60,and 1333 cases were over 60.The mucosa of gastric antrum was collected for H.pylori culture.Drug sensitivity of isolated H.pylori strains was tested with metronidazole,amoxicillin and gentamycin,furazolidone,clarithromycin and levofloxacin.x2 test was performed for the comparison of drug resistance among different age groups.Results Among 6280 specimens,H.pylori culture of 2311 cases was positive (positive rate 36.80％).The positive rate of patients aged between 40 and 60 was the highest (38.43％,1159/3016),and that of patients over 60 was the lowest (33.76％,450/1333).The drug resistance rate of metronidazole in patients between 20 and 40 years reached 98.02％ (644/657) and was the highest.The drug resistance rate of clarithromycin in patents over 60 years old was the highest,which was 22.67％(102/450).The drug resistance rates of the levofloxacin,furazolidone,amoxicillin and gentamicin were low of all ages.Mixed resistance of two antibiotic appeared in 586 strains,mixed resistance rate of metronidazole and clarithromycin was the highest (259 strains,15.23 ％).Mixed resistance of three drugs appeared in 49 strains,mixed resistance rate of metronidazole,clarithromycin and levofloxacin was the highest (32 strains,12.88％).A total of 1691 strains were sensitive to the combination of amoxicillin and gentamycin,the sensitive was rate 99.41％.The sensitive rate of the combination of amoxicillin and furazolidone,gentamycin and furazolidone was both 94.24％.Conclusions Metronidazole presented high drug resistance of all ages,and should not be chosen.The combination use of amoxicillin and gentamicin is the ideal antibiotic combination for different ages.The combination of gentamycin and furazolidone is recommended for patients who are allergic to penicillin.

Objective To explore the effect of pre-filled catheter flushing device on the sealing of venous indwelling needle. Methods One hundred and sixteen patients with venous indwelling needle were assigned into observation group, in which pre-filled catheter flushing device was used to seal the needle tube and another 109 with venous indwelling needle as control group, in which the needle tube was sealed with 50U/mL heparin saline.The two groups were compared in terms of blockage of the tube, phlebitis, catheter-associated infection, indwelling time of the needle and time for nurses to seal it.Results The rates of blockage of catheter, phlebitis and catheter-associated infection of the observation group were all lower than those of the control group.The indwelling time of the needle was significantly longer than that in the control group.The time for nurses to seal the catheter was significantly shorter than that in the control group(all P<0.05). Conclusion Pre-filled catheter flushing device can reduce complication rate and improve working efficiency of nurses.

OBJECTIVE: To study the expression of angiopoietin receptor Tie-2 in the renal tissue of diabetic rats and the effects of Astragalus. METHODS: SD rats were randomly divided into normal control group, diabetes group and Astragalus-treated group. The expression of receptor Tie-2 in the renal tissue was assessed by using real-time quantitative polymerase chain reaction and immunohistochemical method. RESULTS: Glomerule Tie-2 protein expression was significantly elevated in the diabetes group as compared with the normal control group (P<0.01). Glomerule Tie-2 protein expression in the Astragalus-treated group was decreased as compared with the diabetes group (P<0.01). CONCLUSION: Tie-2 may play an important role in the pathogenesis of the early stage diabetic renal injury. The reno-protection effect of Astragalus may be mediated by down-regulating the expression of Tie-2 in the kidney tissue of diabetic rats.

Background:Resistance of Helicobacter pylori(Hp)to antibiotics is the primary reason for failure of Hp eradication therapies. It has been reported that there are regional differences in the resistance rate of Hp to commonly used antibiotics. Monitoring the regional Hp resistance status is helpful for improving the eradication rate in local area. Aims:To investigate the resistant spectrum of clinical Hp strains to commonly used antibiotics in Jiaxing Area,Zhejiang Province. Methods:A total of 17 402 patients who underwent gastroscopy from Jan. 2009 to Dec. 2013 at Jiaxing First Hospital were recruited for Hp culturing by using gastric antral biopsies. Then a drug sensitive test was performed for the Hp strains obtained from the culturing. Sensitivity of these Hp strains to metronidazole,amoxicillin,gentamicin,furazolidone,clarithromycin and levofloxacin was determined. Results:Among the 17 402 cases of gastric antral biopsies,5 898(33. 9% )were positive for Hp culturing. Drug sensitive test revealed that the resistance rate of metronidazole was extremely high in all year and age groups;the resistance rates of clarithromycin and levofloxacin were increasing by years;and the resistance rates of furazolidone,amoxicillin and gentamicin were considerably low in all year and age groups. When two antibiotics were combined,amoxicillin and gentamicin accounted for the lowest resistance rate,and the next were amoxicillin and furazolidone,and gentamicin and furazolidone,respectively. Conclusions:The clinical Hp strains isolated from all age groups in Jiaxing Area,Zhejiang Province are highly resistant to metronidazole,therefore it is not recommended to be used in first line eradication regimen. Amoxicillin combined with gentamicin/ furazolidone is an ideal antibiotic combination for patients with Hp infection in all age groups;and combination of gentamicin and furazolidone is recommended to patients who are allergic to penicillin.