1.Assessment of germ cell apoptosis in cryptorchid rats.
Izzet KOCAK ; Mehmet DUNDAR ; Mine HEKIMGIL ; Pinar OKYAY
Asian Journal of Andrology 2002;4(3):183-186
AIMTo investigate the relationship between germ cell degeneration and apoptosis in cryptorchid rats.
METHODSThirteen 21-day-old Wistar rats were made unilaterally cryptorchid by closing the left inguinal canal. At day 30 (Group 1, n=6) and day 60 (Group 2, n=7) after operation, the testes were removed for histopathological examination. The controls (n=8) were sham operated and were sacrificed at day 60. Germ cell apoptosis was assessed by means of the TUNEL method.
RESULTSSpermatogenesis was arrested and the testicular and seminiferous tubular diameters were significantly reduced In the unilateral undescended testes (UUTs) compared with the contralateral descended testes (CDTs) and the control rats. However, atrophic changes, pathological calcification, necrosis of seminiferous tubule, and absence or sloughing of germ cells were not found in all the animals. The spermatocytes were the main type of germ cells undergoing apoptosis in all the groups. In the UUTs, there was a significant and time-dependent increase in the mean apoptotic index. By 60 days after surgery, increased apoptosis in germ cells was also observed in the CDTs.
CONCLUSIONApoptosis is the predominant mechanism of germ cell death rather than atrophy and necrosis in cryptorchidism.
Animals ; Apoptosis ; Cryptorchidism ; pathology ; In Situ Nick-End Labeling ; Male ; Rats ; Rats, Wistar ; Spermatocytes ; pathology
2.Sclerosing Extramedullary Hematopoietic Tumor Mimicking Intra-abdominal Sarcoma.
Serap KARAARSLAN ; Nalan NESE ; Guray ONCEL ; Nazan OZSAN ; Taner AKALIN ; Hasan KAPLAN ; Filiz BUYUKKECECI ; Mine HEKIMGIL
Journal of Pathology and Translational Medicine 2015;49(4):335-338
No abstract available.
Sarcoma*