1.Effects of Spasticity Therapeutic Apparatus on Talipes Varus after Stroke
Dayong YE ; Ying LIU ; Xin LEI ; Hualian ZHANG ; Minbo ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2010;16(5):459-460
ObjectiveTo observe the effect of Spasticity Therapeutic Apparatus on the talipes varus in ptients with stroke. Methods60 patients were divided into treatment group and control group. Patients in the control group accepted routine therapy, while the treatment group were treated with the Spasticity Therapeutic Apparatus in addition. ResultsThe Clinic Spasticity Index (CSI) of ankle significantly improved after treatment (P<0.05) in both groups, and more in the treatment group than in the control group (P<0.05). ConclusionThe Spasticity Therapeutic Apparatus can facilitate the recovery of talipes varus of the patients with stroke.
2.Optimium extraction of polysaccharides from folium Artemisia argyi by response surface methodology
Xia SHEN ; Yanhong ZHANG ; Huihui YUAN ; Minbo LAN
Chinese Traditional Patent Medicine 2010;(1):48-51
AIM: To optimize the extraction of folium Artemisia argyi polysaccharides by the use of response surface methodoloy.METHODS : Experiment factors and levels were first selected on the ground of one factor test.Along with the central composite experimental design principles,the response surface methodoloy with three factors and three levels was adopted in search of multiple quadratic linear regression.Response surface and contour were finally chosen as the extraction rate and the response value respectively.RESULTS: The optimum extraction conditions of the polysaccharides from folium Artemisia argyi were concluded as follows : extraction temperature was at 99 ℃,extraction time was 2.3 hours,ratio of 20.CONCLUSION : Under these conditions,the yield of folium Artemisia argyi polysaecarides is up to 3.017%,extraction rate of the predictive best polysaccharides is 3.096%,and the relative error is 2.6%.
3.Physical properties and biocompatibility of 3D printed bone microparticle/poly (lactic-co-glycolic acid) scaffold
Minbo ZHANG ; Qifeng PENG ; Yaping MA ; Weijun KONG ; Wenbo LIAO
Chinese Journal of Tissue Engineering Research 2019;23(14):2215-2222
BACKGROUND: 3D printing technology provides possibility for the preparation of an ideal bone tissue engineering scaffold with high individualization, precise regulation of the porosity, pore size and inter-aperture connectivity. OBJECTIVE: To study the physical properties and biocompatibility of allogeneic bone microparticle/poly (lactic-co-glycolic acid) composite absorbable scaffolds fabricated via fused deposition modeling 3D printing. METHODS: 3D printed bone microparticle/poly (lactic-co-glycolic acid) composite absorbable scaffolds were fabricated via fused deposition modeling. The porosity, water absorption, water contact angle and in vitro degradation rate of the scaffold were measured. (1) Cytotoxicity test: Rabbit adipose-derived mesenchymal stem cells were cultured in normal medium (negative control group) and composite scaffold extract (experimental group) for 1, 3, 5, 7 days, and cell counting kit-8 was then used to detect cell proliferation. (2) Hemolysis test: Rabbit anti-clotting was added to physiological saline, distilled water and composite scaffold extract, and the hemolysis rate was detected after 50 minutes. (3) Acute toxicity test: The physiological saline and composite scaffold extracts were intraperitoneally injected into the rabbits respectively to observe the general condition of rabbits and the pathological changes of important organs. RESULTS AND CONCLUSION: (1) The porosity, water absorption and water contact angle of the composite scaffold were (60.86±2.88) %, (53.98±2.04) % and (76.27±0.34) °, respectively. (2) After in vitro immersion in PBS for 4 weeks, the absorbable composite scaffold degraded relatively slowly, and the scaffold morphology remained stable, while the degradation rate accelerated after 4 weeks. The scaffold was almost completely absorbed at about 11 weeks, and it is a relatively ideal absorbable scaffold material. (3) There was no difference in cell proliferation between the experimental group and the negative control group at different time points (P> 0.05) . The cytotoxicity of the absorbable composite scaffold was grade 1. (4) The hemolysis rate of the absorbable composite scaffold was 3.8%, which met the hemolytic requirement of biomedical materials (the hemolysis rate of less than 5%) . (5) Within 72 hours after injection of absorbable scaffold extract, the rabbits showed no acute toxic reaction, as well as no pathological changes in the liver, heart and kidney were observed after 7 days of injection. (6) To conclude, the 3D printed allogeneic bone microparticle/poly (lactic-co-glycolic acid) fabricated via fused deposition modeling 3D printing has preferable physical properties and biocompatibility.
4.Pathologic Diagnosis of a Pituicytoma in a Han-Wistar Rat
Minbo HOU ; Tiantian CUI ; Naying SU ; Miaomiao ZHANG ; Yongmin JIAO ; Jianyan YAN ; Xijie WANG ; Ohira TOKO
Laboratory Animal and Comparative Medicine 2023;43(6):654-658
A case of pituicytoma was observed in a Han-Wistar rat from the control group of a 2-year carcinogenicity study. No obvious abnormality were found in clinical observation and necropsy. Hematoxylin and Eosin (HE) staining results showed that nodular hyperplasia in the pars nervosa of the pituitary, which was well demarcated and compressed the adjacent normal tissue. The tumor cells were similar to the glial cells with round or oval nuclei, cytoplasm rich in eosinophilic or vacuole. The tumor cells differentiated well, with no obvious cell pleomorphism and visible mitotic figures. Some tumor cells were arranged in a pseudorosette formation. Immunohistochemical staining (IHC) analysis confirmed positive expression of Glial fibrillary acidic protein (GFAP) and S-100 protein. The tumor was diagnosed as the spontaneous benign pituicytoma combining the HE and IHC staining results.