1.Interleukin-10 and interleukin-5 balance in patients with active asthma, those in remission, and healthy controls
Minako TOMIITA ; Eduardo CAMPOS-ALBERTO ; Masayuki SHIMA ; Masanobu NAMIKI ; Kazuo SUGIMOTO ; Hiroyuki KOJIMA ; Hiroko WATANABE ; Kunio SEKINE ; Toshiyuki NISHIMUTA ; Yoichi KOHNO ; Naoki SHIMOJO
Asia Pacific Allergy 2015;5(4):210-215
BACKGROUND: The immunological mechanisms of asthma remission remain unclear although several reports have suggested that balance between T helper (Th) 2 cytokines and regulatory cytokines is related. OBJECTIVE: To study the balance between interleukin (IL) 10 and IL-5 in asthma clinical remission. METHODS: We measured the numbers of IL-5 and IL-10 producing cells in peripheral blood mononuclear cells stimulated with mite antigen obtained from patients with active asthma (group A, n = 18), patients in clinical remission (group R, n = 15) and nonatopic healthy controls (group H, n = 14). RESULTS: The numbers of IL-5 producing cells in groups A and R were significantly higher than in group H. The number of IL-5 producing cells was lower in group R than in group A, although the difference was not statistically significant. The number of IL-10 producing cells was higher in group R than in group A, although again the difference was not statistically significant. There was a significant difference in the number of IL-10 producing cells between groups A and H but not between groups R and H. The ratio of the number of IL-10 to IL-5 producing cells was highest in group H followed by groups R and A, and the differences were statistically significant for each pair of groups. CONCLUSION: Our study suggests that the IL-10/IL-5 balance is related to clinical asthma. The balance differs between patients in clinical remission and healthy controls, suggesting that allergic inflammation may continue even after clinical asthma remission.
Asthma
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Child
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Cytokines
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Humans
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Inflammation
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Interleukin-10
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Interleukin-5
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Interleukins
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Mites
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Prognosis
2.Immediate systemic allergic reaction in an infant to fish allergen ingested through breast milk
Takayasu ARIMA ; Eduardo CAMPOS-ALBERTO ; Hiraku FUNAKOSHI ; Yuzaburo INOUE ; Minako TOMIITA ; Yoichi KOHNO ; Naoki SHIMOJO
Asia Pacific Allergy 2016;6(4):257-259
This is a rare case report of systemic allergic reaction to fish allergen ingested through breast milk. Mother ate raw fish more than 3 times a week. Her consumption of fish was associated with urticaria and wheeze in an infant via breast-feeding. Fish-specific IgE antibodies were detected by skin prick test but not by in vitro IgE test. This case demonstrates that fish protein ingested by mother can cause an immediate systemic allergic reaction in offspring through breast-feeding. Although fish intake is generally recommended for prevention of allergy, one should be aware that frequent intake of fish by a lactating mother may sensitize the baby and induce an allergic reaction through breast-feeding.
Allergens
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Antibodies
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Breast
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Fishes
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Humans
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Hypersensitivity
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Immunoglobulin E
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In Vitro Techniques
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Infant
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Milk, Human
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Mothers
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Skin
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Urticaria