BACKGROUND: Postoperative abdominal adhesion is one of the most urgent surgical problems. In view of the complicated pathological mechanisms and various risk factors of postoperative adhesion, surgical techniques and barrier materials have increasingly become the focus of postoperative prevention of adhesion. OBJECTIVE: To summarize the pathological mechanisms and risk factors of postoperative abdominal adhesions and to review the material source, biological characteristics, current research status, and potential deficiencies of different types of barrier materials. METHODS: We retrieved PubMed, CNKI, WanFang and VIP databases from their inception dates to January 2018, and "postoperative abdominal adhesion, etiology and pathogenesis, risk factors, preventive measures, barrier materials" were used as the keywords in English and Chinese, respectively. Fifty-four articles were included in final analysis. RESULTS AND CONCLUSION: Anti-adhesion materials can isolate the injured area and peripheral tissues through physical barriers in the early stage of adhesion formation, and thus prevent the formation of abdominal adhesion. There are three types of anti-adhesion barrier material at present, including solution, gel and membrane agents. Each kind of material has its own advantages and disadvantages. Therefore we explore the pathological process of postoperative abdominal adhesions, predict the risk factors of postoperative adhesions, improve surgical skills and select appropriate anti-adhesion barrier materials according to actual conditions, which are expected to reduce the formation of postoperative abdominal adhesions.

[摘 要] 目的：探讨环化自erb-b2受体酪氨酸激酶2（ERBB2）基因的circ_0007766分子在前列腺癌（PCa）细胞中的作用及缺氧对其表达调控的影响。方法：qRT-PCR检测circ_0007766分子在PCa细胞及组织中的表达；分别转染circ0007766的siRNA至PCa细胞DU145和PC3中，平板克隆实验、CCK-8实验和Transwell 实验检测circ_0007766敲低对PCa细胞克隆形成、增殖、迁移和侵袭能力的影响；厌氧袋法建立DU145和PC3细胞的缺氧细胞模型，WB法检测缺氧通路关键分子HIF-1α的蛋白表达，qRT-PCR检测缺氧模型细胞中circ_0007766分子的表达，WB检测RNA结合蛋白真核翻译起始因子4A3（EIF4A3）的蛋白水平表达，RNA免疫沉淀（RIP）法检测缺氧条件下EIF4A3与circ_0007766的结合，qRT-PCR进一步检测缺氧条件下敲低EIF4A3对circ_0007766表达的影响。结果：circ_0007766在PCa细胞（P < 0.01）及PCa组织（P < 0.05）中呈高表达；敲低circ_0007766（P < 0.05或P < 0.01）能显著抑制PCa细胞的增殖、迁移和侵袭能力（均P < 0.01）。缺氧条件下HIF-1α蛋白表达增加，表明缺氧细胞模型建立成功。qRT-PCR检测结果显示，相较于常氧组，缺氧组circ_0007766的表达显著升高（P < 0.01），WB法检测结果显示缺氧组细胞中EIF4A3 的蛋白表达增强。RIP实验结果显示，circ_0007766在EIF4A3富集组高度富集（P < 0.01）。qRT-PCR检测结果显示，缺氧可显著促进circ_0007766的表达，同时，敲低EIF4A3分子可显著降低缺氧诱导的circ_0007766的表达（P < 0.05或P < 0.01）。结论: circ_0007766在PCa细胞中扮演着促癌分子的角色，其表达形成与缺氧条件下EIF4A3分子的调控有关。