1.Several problems in diagnosis and treatment of chronic cough in children: intensive reading of the guideline for diagnosis and treatment of chronic cough in pediatrics (III).
Chinese Journal of Pediatrics 2008;46(12):939-941
Child
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Chronic Disease
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Cough
;
diagnosis
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therapy
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Guidelines as Topic
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Humans
;
Pediatrics
3.Rational management of refractory Mycoplasma pneumoniae pneumonia.
Chinese Journal of Pediatrics 2013;51(10):724-728
Anti-Bacterial Agents
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administration & dosage
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therapeutic use
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Anti-Inflammatory Agents
;
administration & dosage
;
therapeutic use
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Bronchoscopy
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Child
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Glucocorticoids
;
administration & dosage
;
therapeutic use
;
Humans
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Mycoplasma pneumoniae
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drug effects
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Pneumonia, Mycoplasma
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pathology
;
therapy
4.Airway Neutrophil Activation and Protease Imbalance in Airway of Children with Foreign-Body Aspiration
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To observe neutrophil activation and protease imbalance in airway of children after foreign-body aspiration(FBA).Methods Bronchoalveolar lavage fluid(BALF) was obtained through fiberoptic bronchoscopy.The supernatant was assayed for the concentration of neutrophil elastase(NE) using enzyme-linked immunosorbent assay(ELISA) and elastase inhibition capacity(EIC)/free elastase activity using a colorimetric assay.The cell pellet were smeared for the expression of ?1-antitrypsin(?1-AT) using cellular immunohisto-chemistry and cytologic analysis by Wright-Giemsa stain.Results Percentage of neutrophil,concentration of NE and the expression of ?1-AT in BLAF group,pair group and pneumonia group significantly increased compared with that of control group(Pa0.05).The ratio of Lib NE in FBA,par and peumcria group was 30.3%,27.3% and 22.7%.Conclusions FBA induces similar airway neutrophil activation and protease imbalance to bacterial pneumonia.The contralateral airway showes the same change as the foreign body side.
5.The cloning and expression of an anti-Alzheimer's disease neuroprotective peptide gene humanin
Benyan LUO ; Min YUAN ; Min TANG ; Zhi CHEN
Chinese Journal of Neurology 2001;0(03):-
Objective To use gene engineering technique to express the humanin.Methods Humanin segment was synthesized and cloned into the prokaryocyte expression vector pGEMEX-1 and pet44a. The plasmid was transformed to E. coli BL21(DE3) by screening and identifying to obtain the high expressing positive clone.Results The humanin segment was cloned into vector successfully,and expressed efficiently with the inducement of IPTG. The molecular weight of the expressed recombinant protein of pGEMEX-1-humanin was about 28 kd while it was 63 kd in pet44a-humanin,and the expressed quantity of fusion protein was 40 percentage of the total bacterial protein while it was 20 percent in pet44a-humanin.Conclusion The humanin has been cloned and expressed successfully and the high efficiently expressed recombinant protein might contain the intact important structure domain of the humanin.
6.Treatment of 7 patients with medial meniscal cyst under arthroscopy.
Yan-guo HE ; Min WEI ; Zhi-qiang ZHANG ; Chao CHEN
China Journal of Orthopaedics and Traumatology 2014;27(8):642-644
OBJECTIVETo investigate clinical effects of arthroscopy in the treatment of medial meniscal cyst.
METHODSFrom June 2011 to January 2013, 7 patients with medial meniscal cyst were treated with arthroscopy. There were 3 males and 4 females,ranging in age from 27 to 63 years old,with a mean age of (43.93±2.10) years old. The cysts have been discovered for 3 to 30 months,with a mean time of (10.6±1.3) months. All the patients complained of knee pain,especially in the medial joint gap. The Pisani sign, Caklin sign and medial McMurray sign were all positive. Preoperative MRI examination confirmed the diagnosis. Lysholm score changes and clinical efficacy were observed through a six-month follow-up.
RESULTSThe postoperative Lysholm scores were all significantly higher than the preoperative scores. According to Sarimo standard, 6 patients got an excellent result, and 1 good.
CONCLUSIONArthroscopic treatment of medial meniscal cyst has replaced the traditional method, which could retain the normal meniscus as much as possible and repair the meniscus injury simultaneously, as well as get a good curative effect and a good recovery of knee function. This method is worthy of clinical application.
Adult ; Arthroscopy ; methods ; Cysts ; physiopathology ; surgery ; Female ; Humans ; Male ; Menisci, Tibial ; physiopathology ; surgery ; Middle Aged
7.CD23 and CD19 expression on peripheral blood lymphocytes in asthmatic children.
Yong-qin ZHU ; Man-li KANG ; Zhi-min CHEN
Chinese Journal of Pediatrics 2003;41(7):554-554
Antigens, CD19
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analysis
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Asthma
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blood
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Child
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Child, Preschool
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Female
;
Flow Cytometry
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Humans
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Immunoglobulin E
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blood
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Lymphocytes
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immunology
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Male
;
Receptors, IgE
;
analysis
8.Clinical and neuroimagings analysis in 10 patients with lupus encephalopathy
Zhi LIU ; Lin CHEN ; Liying CUI ; Min QIAN ; Xiaoguang LI
Chinese Journal of Neurology 2012;45(9):664-668
Objective To report specific clinico-radiological syndromes in neuropsychiatric systemic lupus erythematosus (NPSLE).Methods Ten patients with NPSLE in Peking Union Medical College Hospital from 2005 to 2011 were studied retrospectively with magnetic resonance imaging, computer tomography or positron emission tomography. Results Posterior reversible encephalopathy syndrome was diagnosed in 2 patients with radiological features,headache and tonic-clonic seizure;3 patients with bilateral diffuse leukoencephalopathy,cognitive disorder and acute confusional state; 1 Fahr' s disease patient,with cognitive disorder and psychiatric symptom,movement disorder; 2 Parkinsonism patients with tremor and cogwheel rigidity,and 2 chorea patients. Conclusions The emergence of diffuse brain calcinosis,leukoencephalopathy and edema may happen in lupus encephalopathy particularly.Autoantibody reaction and vascular disease may play an important role in movement disorder including Parkinsonism and chorea.
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