Endothelial Cells ; metabolism ; pathology ; Humans ; Sepsis ; metabolism ; physiopathology

Objective To compare the clinical value of fluid attenuated inversion recovery imaging (FLAIR),magnetic resonance diffusion-weighted imaging(DWI) and magnetic resonance angiography(MRA) in diagnosis of early cerebral infarction.Methods 70 cerebral infarction patients were chosen.They underwent FLAIR,DWI and MRA examination.Results 70 patients with early cerebral infarction mostly were multiple lessions accouting for 60％.Supper actue period had 22 cases and acute period had 48 cases.DWI had the highest detection rate of cerebral infarction,particularly in the hyperacute period the detection rate reached 100％,which was significantly higher than the FLAIR and MRA(x2 =12.4,P ＜ 0.05).Conclusion Especially in the early diagnosis of hyperacute cerebral infarction,DWI is superior to FLAIR and MRA.FLAIR showed better on the cortex and periventricular lesions,MRA can check out the blood vessel lesions in the infarction area.

Accidents, Occupational ; prevention & control ; Emergency Medical Services ; organization & administration ; Humans ; Occupational Exposure ; prevention & control ; Occupational Health ; Poisoning ; prevention & control ; Risk Assessment

Adolescent ; Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; epidemiology ; Young Adult

Objective:To investigate the changes and clinical significances of serum levels of granulocyte macrophage colony-stimulating factor (GM-CSF),interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels in patients with rheumatoid arthritis (RA).Methods:126 cases of patients with RA in our hospital from June 2013 to December 2016 were selected as RA group and divided into two subgroups as active RA group (69 cases) and remission RA group (57 cases) according to the severity of the disease.55 healthy cases who receive physical check in our hospital at same time were selected as the control group.The serum GM-CSF,IL-6 TNF-α levels were detected by enzyme linked immunosorbent assay (ELISA) and compared between different groups,the correlation of serum GM-CSF,IL-6 TNF-α levels with DAS28 were analyzed.Results:The serum GM-CSF,IL-6 TNF-α levels of RA group were significantly higher than those of the remission RA group and control group (P＜0.01),and the serum GM-CSF,IL-6 TNF-α levels of remission RA group were also significantly higher than those of the control group (P＜0.01).Positive correlation between serum GM-CSF,IL-6 and TNF-α levels were found(P＜.0.01).The level of DAS28 was significantly correlated with the levels of serum GM-CSF,IL-6 and TNF-α (r=0.473,0.584,0.675,all P＜0.01).Conclusion:The levels of serum GM-CSF,IL-6 and TNF-α were up-regulated in patients with RA and positively correlated with the activity of RA,which might be of important clinical significance in the early diagnosis,disease evaluation and prognosis of RA.

Objective To investigate the expression of phosphatidylinositol 3-kinase(PI-3K)in adipose tissue of polycystic ovary syndrome patients(PCOS),and explore molecular mechanisms of insulin resistance(IR)in PCOS.Methods Samples from patients with PCOS with IR(n=19),PCOS without IR (n=10)and controls(n=15)were collected.Serum fasting insulin(FIN)and fasting plasma glucose (FPG)were measure& Insulin resistance index was calculated using homeostasis model assessment (HOMA)to analyze the relationship between these markers and IR.Western blot technique was used to detect the PI-3K p85 subunit.Gene expression of PI-3K p85 subunit was detected by reverse transcription polymerase chain reaction(RT-PCR)method.Kinase activity was detected by immunoprecipitation,thin- layer chromatography and gamma scintillation counting.Results(1)The levels of FIN[(25.2?3.8) mU/L]and HOMA-IR(1.6?0.3)in PCOS with IR were significantly higher than those in PCOS without IR[(13.4 +3.8)mU/L,0.9?0.3]and controls[(9.5 +2.6)mU/L,0.5?0.3;all P＜0.05).(2) There was no significant difference in the protein(0.65?0.10)and gene expression(0.92?0.12)of PI-3 K p85 subunit in PCOS with IR compared with PCOS without IR(0.72?0.10,1.01?0.10)and control groups(0.73?0.14,1.00?0.12;P＞0.05).(3)PI-3K activity in PCOS with IR(81%)and PCOS without IR(89%)was significantly decreased(P＜0.01,P＜0.05)and negatively correlated with HOMA- IR(r=-0.69,P＜0.01;r=-0.62,P＜0.05).Conclusions No significant difference in the protein and gene expression of PI-3K p85 subunit in PCOS with IR is found.The decreased PI-3K activity may lead to IR of PCOS.

Hippophae rhamnoides is one of the most representative economy crops for its wide uses of biological diversity and abundance of resource. As the key healthy food development and ecology protection, H. rhamnoides has been developed widely. Meanwhile, the development of H. rhamnoides has obtained great achievements. Nowadays, H. rhamnoides is still a necessary economy crop, while it has great influence on ecology protection. This paper discussed the phytochemistry, pharmacology, clinical application and product development, and propounded some suggestions for future research and economy development to get comprehensive benefit of H. rhamnoides and to serve for well-off society.
Biomedical Research ; methods ; trends ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Flavonoids ; chemistry ; therapeutic use ; Hippophae ; chemistry ; Humans ; Molecular Structure ; Phytotherapy ; methods ; trends ; Polyphenols ; chemistry ; therapeutic use

This article was aimed to study the dynamics of biocontrol endobacteria in roots, stems and leaves of Panax ginseng and soils adjacent roots. Gradient screening and root irrigation methods were used to develop Rif-resistance bacteria and inoculation. Dilution plate method was used to count the number of them in roots, stems and leaves of P. ginseng and soils adjacent roots. The results showed that bacteria which can endure 120 μg·mL-1 Rif was developed, and no change was found on their antagonistic activity against A lternaria panax Whetz and Phytophthora cactorum. In roots and stems of P. ginseng, ge15 and ge25 showed the highest number in the initial.Then, populations of ge15 and ge25 in ginseng roots and stems decreased to a stable status gradually. In ginseng leaves, populations of ge15 and ge25 increased to the highest 3 days after inoculation, then, populations of them were decreased and kept at certain concentration. In ginseng rhizosphere soil, populations of ge15 showed reciprocating change, and which of ge25 showed increase initial, and then decrease to a stable status. It was concluded that endobacteria can keep a certain population in ginseng roots, stems, and leaves. Biocontrol agents based on them will be helpful for ginseng diseases controlling in the field.

By nutrient solution culture method, effects of benzoic acid, diisobutyl phthalate, diisobutyl succinate, palmitic acid, 2,2-bis(4-hydroxyphenyl) propane on root exudates of Panax ginseng were studied. GC-MS results in-dicated that, 15 to 23 components were detected in treatments including control, which were organic acids, alcohol, amino acid esters, phenols, amines, condensed nucleus, saccharine, etc. Through compared with control we found that, phenol and phenolic acids in root exudates increased in most of stress treatments. So we deduced that, the re-laxation of autotoxins from roots to outside environment was stimulated by autotoxins released into soil, and which ac-celerated the autotoxicity to ginseng seedlings.

Objective:To investigate the effect of Bitongling Granule medicated serum on rabbit articular chondrocytes cultured in vitro.Methods:Bitongling Granule(2ml/kg) was given to New Zealand rabbits for 3 days for preparation of containing serum.Rabbit.chondrocytes were divied into Bitongling Granule groups of different concentrations,blank serum group,experimental control group and anti-bone capsule group.To observe the change of nitric oxide synthase and superoxide dismutase in all groups.Results:Bitongling Granule can reduce the activity of NO and increase the level of SOD in sera.Conclusions:Bitongling Granule can prevent and cure experimental osteoarthritis,which is related to the decrease of NO and activation of SOD.