AIM:To investigate the effects of ursolic acid ( UA) on the migration and invasion of human lung cancer cell line A549, and to explore its mechanism .METHODS:The cell viability was detected by MTT assay .The ex-pression of miRNA-133a was detected in the A549 cells treated with UA by real-time PCR.The miRNA-133a mimics and inhibitor were transfected into the A 549 cells, and the transfection efficiency was analyzed by real-time PCR.The cell mi-gratory and invasive abilities were determined by wound healing and Transwell methods , respectively .RESULTS:The via-bility of the human lung cancer A549 cells was significantly inhibited by UA in a dose-dependent manner (P<0.05).IC50 of UA (24 h) for lung cancer A549 cells was 31.04 μmol/L.UA treatment significantly inhibited the migratory and inva-sive abilities of A549 cells in a concentration-dependent manner , accompanied by significantly elevation of miRNA-133a expression.The mimics and inhibitor of miRNA-133a significantly upregulated and downregulated the expression of miRNA -133a in the transfected A549 cells, respectively.In addition, the viability of the A549 cells was decreased extremely after tansfected with the miRNA-133a mimics (P<0.01), so did the results of the cell migration and invasion test .The A549 cells tansfected with the miRNA-133a inhibitor showed an opposite changes of the cell viability , migration and invasion . CONCLUSION:UA inhibited the viability , migration and invasion of lung cancer A 549 cells by elevating the expression of miRNA-133a.

Objective To evaluate the safety, efficacy and morbidity of transurethral electrovaporization of superficial bladder tumor (TVBt). Methods TVBt was performed in seventy-three pat iens (mean age 66 years) with superficial bladder carcinoma,twelve of them trans urethral electrovaporization of the prostate (TVP) was carried out simultaneousl y for concomitant BPH. Results The tumor was resected in one-stage for 72 cases,in two-stage for multiple tumors in one patient. The mean operative time was 29 minutes in simple TVBt group, and 78 minutes in the group performing both TVBt and TVP. Intraoperative bladde r perforation occurred in 1 case, No patient need blood transfusion for bleeding . The mean duration of an indwelling urethral catheter was 46h. All patients wer e followed up for twenty-four months, and the recurrence rate was 21% within t wo years after the operation. Conclusions TVBt is a simp le, effective and safe surgery for the treatment of superficial bladder cancer.

Objective To evaluate endoscopic management of bladder neck obstruction (BNO). Methods 44 patients suffering from BNO have been treated by transurethral resection of bladder neck (TURN). Results All patients have been followed up for 6～84 months with a mean of 48 months.The success rate has been 84%.Recurrence was observed in 4 (11%) for all of which TURN was carried out twice or three times with success.The causes of the 7 failures were neurogenic bladder, detrusor-extra-sphincter dyssynergy, BNO with chronic prostatitis (CP) or BNO with chronic cystitis in female patients. Conclusions Endoscopic management is a safe and efficient treatment for BNO.Careful consideration should be undertaken in case there is CP or for female patients.Postoperative adjunctive hormone supplement therapy and long-period urethral dilatation might be needed for preventing recurrence.

The hemodynamic effects of deliberated hypotension induced by PGE1 or SNP on 14 dogs anesthetized by ketamine were studied separately.MAP was decreased by 30%~40% after infusing PGE,or SNP Hemodynamic variables were measured before and 15 min 30 min after hypotension and 15 min after the discontinuation of infusion.The results indicated that SVRI, PVRI, MPAP, LVSWI and RVSWI all decreased significantly during PGE1-or SNP-induced hypotension. CVP decreased and HR increased significantly during SNP-induced hypotension, but with no significant change during PGE1-induced hypotension and with SVI. CI. PCWP remained unchanged in these two groups throughout the experiments.

Objective To observe the therapeutic effect of pericarpium zanthoxylum oil on experimental hyperlipemia in mice. Methods Hyperlipemia model was reproduced in mice, and then pericarpium zanthoxylum oil was garaged for 4 weeks. Blood samples were obtained from retro-ocular vessel. The levels of blood lipids were determined, the hemorrheology parameters were determined by hemorrheometer, and the pathological changes in the liver of mice were observed microscopically. Results Zanthoxylum oil could obviously reduce contents of TC, TG, LDL-C (P

AIM:To investigate the regulation of miR-222 on BCL2L13 gene and its effect on the growth and apoptosis of HBx-HepG2 cells, and to explore the underlying molecular mechanisms .METHODS:The expression level of miR-222 was detected by RT-qPCR.The HBx-HepG2 cell growth was examined by MTT and colony formation assays .The cell cycle and apoptosis were analyzed by flow cytometry .The recombination vector pmirGLO-BCL2L13 was constructed, and dual-luciferase reporter experiment was performed to validate the target of miR-222.RESULTS:The expression level of miR-222 in the HBx-HepG2 cells was significantly higher than that in the L 02 cells ( P<0.05 ) .Over-expression of miR-222 enhanced HBx-HepG2 cell growth, changed cell cycle, and inhibited apoptosis (P<0.05).Knockdown of miR-222 reduced HBx-HepG2 cell growth, changed cell cycle, and increased cell apoptotic rate (P<0.05).BCL2L13 was down-regulated in the HBx-HepG2 cells as compared with L02 cells (P<0.05), and knockdown of miR-222 in the HBx-HepG2 cells increased the expression level of BCL2L13 (P<0.05).The results of dual-luciferase reporter assay and re-store experiment showed that miR-222 negatively regulated the expression of BCL2L13 via targeting 3’ UTR of BCL2L13, resulting in the promotion of HBx-HepG2 cell growth .CONCLUSION: miR-222 enhances HBx-HepG2 cell growth via down-regulation of BCL2L13.

Objective To evaluate the effectiveness of biofeedback for anxiety patients by carrying out a Meta-analysis.Methods We searched CNKI,VIP,CBM(1989 to 2013) to identify relevant randomized-control trials applying biofeedback for anxiety patients.We used Review Management (RevMan) 5.0 software (provided by the Cochrane collaboration) to conduct Meta-analysis.Results We included 11 studies.Meta-analysis for the biofeedback group vs.the control group showed that biofeedback was significantly superior to control (SMD=-3.00,95％CI:-4.40～-1.61); Meta-analysis for the biofeedback plus drug group vs.drug group showed that biofeedback plus drug was significantly superior to drug (SMD=-1.33,95％CI:-1.82～-0.85).Conclusions We found evidence that biofeedback (applying alone or combined with drugs) is effective in improving anxiety symptom.But all the studies had methodological limitations,our conclusion needs further studies with strict design,high quality and consistent assessment tools to be expounded and proven.

Objective To investigate the localization,zone and activation intensity of olfactory center in young versus elderly healthy volunteers by functional magnetic resonance imaging (BOLD-fMRI),so as to elucidate the effect of age on olfactory center in healthy population.Methods Thirteen right-handed healthy adult volunteers were recruited and divided into two groups:young group (5 males and 3 females,mean aged 23 years) and elderly group (2 males and 3 females,mean aged 69.2 years).The olfactory stimulus was r-undecalactone,and it was given according to a block design.The fMRI detection was performed on Philips Achieva 3.0 T MR scanner,and data of BOLE-fMRI was processed and analyzed to get cerebration image by using SPM2.Results In groupaveraged maps,both young and elderly group showed significant olfactory activation in right parahippocampal gyrus,left hippocampal sulcus,right and left superior temporal gyrus,etc,subcortical activation in right thalamus,dorsal pons,and cerebellum activation in cerebellar vermis.Activations in right inferior frontal gyrus,right middle frontal gyrus,right medial occipito-temporal gyrus and right fimbria of hippocampus were observed only in young group,while activation in bilateral middle temporal gyrus was observed only in elderly group.Activation area was apparently smaller and activation degree was lower in elderly group than in young group.Activation intensity in right superior parietal lobule and bilateral superior temporal gyri was higher in male group than in female group (t=13.7,6.08,5.36,respectively,all P＜0.001).Conclusions The intensity of activation in olfactory center is lower in the elderly than in the young,and absence of part of the active regions is found in the elderly,which demonstrates the regression of olfactory center in the elderly.The olfactory center shows right-predominant activation,and olfactory activation intensity in some cortical regions is higher in males than in females.

Objective To evaluate the effect of procaine on prevention of skin slap necrosis after modified radical mastectomy in breast cancer.Methods 106 breast cancer patients were randomly divided into treatment and control groups.In the treatment group,procaine(1% 100ml diluted in 300ml mormal saline,42℃)were compressed under the skin flap in operation,the control group received same amount of normal salin instead.The rates and sizes of skin flap necrosis were obeserved and compared.Results There were 13 cases suffured from skin flap necrosis among 106 breast cancer cases who received surgery operation.The rate of the flap necrosis in treatment group was5.76%(3/52),and the control group was 18.51%(10/54),there was significant difference(P

Objective To evaluate the clinical efficacy of 1 aparoscopic anatomical hepatectomy (LaHt)in the treatment of liver cancer by comparing with Open precise hepatectomy (OHt).Methods Forty-six cases of liver tumor hepatectomy were collected by the none randomized controlled trails (non-RCTs) from January 2011 to December 2013 in Department of Hepato-Biliary-Pancreatic Surgery,Nanjing Drum Tower Hospital.According to the operation method,they were divided into two groups,including 19 cases of LaHt group and 27 cases of OHt group.Compare differences of their operation time,intraoperative blood loss,time of Pringle maneuver,and postoperative hospital stay,time of postoperative gastrointestinal function recovery,Serum liver function index,pathological tumor margin,postoperative complications,total hospitalization expenditures.Results (1) No perioperative mortality was observed in the 2 groups.(2) Compared with OHt group,LaHt group had shorter Pringle maneuver time with statistical significance (P ＜ 0.05,F =8.662) ; while,LaHt group also had less hemorrhage and shorter operation time than OHt group,although statistical significance did not existed (P ＞ 0.05,F =1.481,F =0.539).(3) Compare with OHt group,postoperative gastrointestinal function recovery of LaHt group was faster while postoperative length of hospital stay of LaHt group was shorter with obviously statistical significances existed (P ＜ 0.01,F =7.691,F =11.408).(4) The liver function index serum ALT,AST of LaHt group is lower compared to that of OHtgroup postoperative dayl and 3,however,only the day 3 AST had statistical significance (P ＜ 0.05,F =4.226).LaHt group had lower CRP level than OHt group on both day 1 and 3 after surgery,but there was no statistical significance(P ＞ 0.05,F =0.792,F =0.007).Serum TB and DB varied little between LaHt and OHt group on both day 1 and 3 after surgery without statistical significance (P ＞ 0.05,F =2.002,F =0.021,F =0.442,F =1.392).(5) The average hospital charges for the patients in LaHt group is higher than that in OH group,but there was still no statistical significance (P ＞ 0.05,F =0.046).(6) Although there was no significant difference between LaHt and OH in positive/negative excision margin ratio (P ＞0.05,x2 =1.453),there was 1 case of excision margin residues in LaHt group which would have negative effects on the prognoses.Conclusions Laparoscopic hepatectomy is a safe and feasible surgical approach,with minimally invasive advantage for treatment of liver cancer.However,compared with open precise hepatectomy,there are still some deficiencies in terms of radical curative aspect.