Objective To explore the effect of oral motor therapy on dysarthria in children with cerebral palsy. Methods 65 cerebral palsychildren with dysarthria were divided into observation group and control group. All the children received basic rehabilitation, and the observation group received oral motor therapy additionally. The articulation of children was observed before and after treatment. Results Theefficiency rate of the observation group (84.8%) was significantly higher than that of the control group (15.6%) (P<0.001). Conclusion Oral motor therapy is effective on dysarthria in children with cerebral palsy.

Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Humans ; Medicine, Chinese Traditional ; methods ; Neoplasms

OBJECTIVETo study the effect of beta-hydroxyisovaleryl shikonin (beta-HIVS) combined cisplatin on activities of ovarian cancer cell line SKOV3 in vivo and its possible mechanisms.

METHODSCells were divided into the blank control group and six beta-HIVS groups (2 - 30 micromol/L). Effect of beta-HIVS at different concentrations on the activities of ovarian cancer cell line SKOV3 was detected using MTT assay. SKOV3 cells were treated with cisplatin (10, 20, and 40 micromol/L) and beta-HIVS (0.25, 1, and 2.5 micromol/L) combined cisplatin. Effect of beta-HIVS combined cisplatin on the activities of ovarian cancer cell line SKOV3 was determined by MTT assay. The expression of Bcl-2 and Bax after treated by different concentrations of beta-HIVS was detected by Western blot.

RESULTSThe activities of SKOV3 were inhibited by different concentrations of beta-HIVS dose-dependently. The 50% inhibition rate (IC50) was 7.37 micromol/L. There was statistical difference in IC50 between each concentration beta-HIVS group and the blank control group (P < 0.05). There was statistical difference in IC50 between the beta-HIVS (1 and 2.5 micromol/L) combined cisplatin groups and the cisplatin group (P < 0.05, P < 0.01). The synergistic effect on beta-HIVS showed dose-dependent manner. Results of Western blot showed beta-HIVS at different concentrations (5, 7.5, and 10 micromol/L) could obviously up-regulate the expression level of Bax protein and inhibit the expression level of Bcl-2 protein, showing statistical difference when compared with the control group (P < 0.01). CONCLUSIONS; HIVS could obviously inhibit in vitro growth of SKOV3 in a dose-dependent manner. With the range of concentration, beta-HIVS showed synergetic effect with cisplatin. Besides, along with increasing beta-HIVS concentrations, the synergetic effect was more significant. The synergetic effect might accelerate the apoptosis of SKOV3 through up-regulating Bax expression and inhibiting Bcl-2 expression.

Apoptosis ; drug effects ; Cell Line, Tumor ; Cisplatin ; pharmacology ; Female ; Humans ; Naphthoquinones ; pharmacology ; Ovarian Neoplasms ; metabolism ; pathology ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; bcl-2-Associated X Protein ; metabolism

OBJECTIVE: To study the mechanism of herbal application along meridians for treatment and prevention of asthma by using serum pharmacological test to observe the effects of serum containing herbs against the constriction of tracheal spiral strips induced by acetylcholine chloride (Ach). METHODS: Guinea pigs were randomly divided into normal control group, normal saline (NS) application group, aminophylline application group, aminophylline injection group, 1-day herb application group, 7-day herb application group and 14-day herb application group. Asthma was induced by Hutson's method in guinea pigs except the normal control group. Guinea pigs in herb application groups were treated by external application of a compound herbal medicine 60 min once every day. Guinea pigs in NS application group were treated by external application of NS. Guinea pigs in the two aminophylline-treated groups were treated by external application and intraperitoneal injection of aminophylline at a dose of 400 mg/kg, respectively. The guinea pigs were killed and the sera were obtained after 1-day, 7-day and 14-day treatment in the three herb application groups, 7-day treatment in the NS application group, the aminophylline application and injection groups, respectively. Serum pharmacological method was used to do the experiment, the effects of different sera on the constriction of tracheal strips were observed, and the constriction rates were calculated. RESULT: The serum containing herbs had an effect in reducing the constriction of tracheal spiral strips induced by Ach, and the effect was similar to that of the serum obtained from the aminophylline injection group. The constriction rate of the tracheal spiral strips was decreased when herbal application treatment was prolonged within a period of time, and it became stable when herbal application treatment was between 7-14 days. The constriction of tracheal spiral strips induced by Ach could be reduced remarkably when it was previously treated by serum containing herbs. CONCLUSION: The anti-acetylcholine function with a time-dependent effect is one of the mechanisms of herbal application treatment along meridians for asthma, and furthermore, herbal application treatment along meridians might be useful for preventing asthma.

Objective To observe the effect of psychological support therapy on anxiety resulted from pain after stroke. Methods 40 patients with anxiety after stroke were divided equally into psychological support treatment group and control group. They were assessed with Hamilton Anxiety Scale (HAMA) and Visual Analogue Scale (VAS) of pain before and after treatment. Results The scores of HAMA and VAS improved more in treatment group than in control group (P<.01). Conclusion The psychological support therapy can reduce the anxiety and pain after stroke.

Occupational therapy is an important part of rehabilitation medicine. There is movement, feel, swallowing, speech, vision,cognitive, psychological and social relations dysfunction in stroke patients. Occupational therapy play an important part in the whole processof intervention for stroke patients, and help them improve their quality of life.

ObjectiveTo study the effect of Tetrax balance test and exercise on the balance function of stroke patients with hemiplegia. Methods50 stroke patients with hemiplegia and with disorder of balance function were divided into treatment group (25 cases) and control group (25 cases). The patients in both groups received regularly rehabilitation train and medical therapy. The treatment group was given balance train and motor function train responded to the analysis of the Tetrax balance assessment as well. They were evaluated with Berg balance scale, motor function of lower extreme in Fugl-Meyer Assessment (FMA) and activities of daily living (ADL) before and 4 weeks, 8 weeks, 12 weeks after treatment respectively. ResultsThere was no difference in balance function and motor function of lower extreme and ADL between treatment group and control group before treatment (P>0.05), but 4 week, 8 week, 12 week after treatment, there were significant improvement for balance function and motor function of lower extreme in both groups (P<0.01), and the treatment group improved more significantly than those of control group (P<0.05).ConclusionTexrax balance assessment can help understand the obstacle in balance function and formulate more suitable rehabilitation treatment.

ObjectiveTo assess the effects of constraint-induced movement therapy(CIMT) on sub-acute stroke patients with upper extremity motor dysfunction.Methods63 patients with hemiplegia after stroke were divided into two groups: control group(31 cases) and CIMT group(32 casese). The two groups received the regular rehabilitation training for 14 d. Then the control group went on the the regular rehabilitation training, while the CIMT group received CIMT for 14 d. All patients were assessed by the Action Research Arm Test(ARAT) and Fugl-Meyer(FMA) on the first day, the 15th day and the 30th day after the treatment.ResultsARAT score and FMA scores were higher in CIMT group than in the control group 30 d after the treatment and in CIMT group 15 days after the treatment(P<0.001).ConclusionCIMT is more effective to improve the upper limb motor function of the sub-acute stroke patients than the regular rehabilitation training.

To study the chemical constituents from the bark of Myrica rubra, fourteen compounds were isolated from the methanolic extract using various chromatographic techniques, including silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified on the basis of chemical properties and spectroscopic data, as 3, 5-dimethoxy-4-hydroxymyricanol (1), myricanol (2), myricanone (3), myricanol 11-sulfate (4), myricitrin (5), quercetin (6), quercetin-3-rhamnoside (7), tamarixol (8), uvaol (9), ursolic acid (10), taraxerol (11), myricadiol (12), β-sitosterol (13) and β-daucosterol (14). Among them, compound 1 is a new compound, named as 3, 5-dimethoxy-4-hydroxymyricanol, compounds 8, 9 were isolated from the genus Myrica for the first time.
Diarylheptanoids ; chemistry ; isolation & purification ; Myrica ; chemistry ; Phytochemicals ; chemistry ; isolation & purification ; Plant Bark ; chemistry

Objective To observe the influence of hemoperfusion(HP) on microinflammatory state and atherosclerosis in uremic patients .Methods Thirty‐six patients with uremia were randomly assigned into 2 groups ,18 cases in eath group .The hemo‐dialysis(HD) group took hemodialysis for 3 times per week ,4 h per time;the HP+ HD group took once HP per week on the basis of HD .The levels of C‐reactive protein(CRP) ,total cholesterol(TC) ,triglyceride(TG) ,urea nitrogen(BUN) and serum creatinine (Cr) were measured before therapy and in six months after therapy .The atherosclerotic plaque size was detected by ultrasound with fine resolution .Results The levels of CRP ,TG and TC after treatment in the HP+ HD group were significantly decreased com‐pared with those before treatment and the HD group (P<0 .05) .The number of therosclerotic plaques in the HP+ HD group had no increase .BUN and Cr had no statistical differences between the two groups(P>0 .05) .Conclusion HP can alleviate the inflam‐matory reaction and decrease the atherosclerosis occurrence .