Objective To evaluate the clinical efficacy of neo-adjuvant chemotherapy in the treatment of cervical cancer. Methods A total of 32 patients,of which 12 cases of Ⅰ b2,8 cases of Ⅱ a,5 case of Ⅱ b and 7 cases of Ⅲ, were all treated with 2 - 3 cycles of PF chemotherapy before treatment with cisplatin 100 mg/m2, d 1;5-fluorouracil 1000 mg/m2 ,d1-5. The chemotherapy was performed for 3 weeks followed by three weeks rest as one cycle. Results Short-term effect was 81.3% (26/32), with 3 cases of Ⅰ b2 and 1 cases of Ⅱ a had been completely relieved after 2 - 3 cycles of chemotherapies. Simultaneously, chemotherapy improved the removal rate of surgery. Among these 32 patients, there were 4 cases achieved complete response (CR) ,22 cases achieved partial response (PR) ,5 case of stable disease ( SD), 1 cases of progressive disease(PD). The overall response rate( CR +PR) was 81.3% (26/32) ,and the control rate ( CR + PR + SD) was 96. 9% (31/32). The main adverse events included myelosuppression and gastrointestinal response, which were all tolerable. Conclusions The neo-adjuvant chemotherapy reduced the size of cancer,which can improve removal rate of surgery and the quality of life.

Objective To explore the effects of hypericin on inhibition of hepatitis 15 virus (HBV) replication.Methods The concentration gradient hypericin was added to HepG2.2.15 cell culture system and lamivudine was used as control.Enzyme-linked immunosorbent assay (ELISA) and Southern blot were used to examine HBsAg,HBeAg and HBV DNA level in the culture supernatant,respectively.Half inhibitory concentration (IC50) and half effective concentration (EC50) of hypericin were calculated.The effects of hypericin on HBV DNA polymerase were detected by 32p marked deoxy-ribonucleoside triphosphate as the substrate. Independent sample t-test and single factor analysis of variance were used to compare the data between two groups and among multiple groups.Results The inhibition rates of hypericin on HBsAg, HBeAg were enhanced with hypericin concentration increasing and those were higher than lamivudine control group when the concentration was higher than 0.5 μmol/L (t=-0.127,P＜0.05).Southern blot confirmed that hypericin was stronger in inhibition of HBV DNA (EC50=0.2 μmol/L) than lamivudine (t=-0.058,P＜0.05).Hypericin was non-toxic on HepG2.2.15 cells in the range of test with EC50 of 0.2 μmol/L and IC50 of 200 μmol/L.Hypericin did not act on HBV DNA polymerase which was quite different from lamivudine.Conclusions Hypericin can effectively inhibit HBV replication as well as antigen synthesis and is non-toxic on HepG2.2.15 cell.The anti-HBV target of hypericin is different from nucleos(t)ide analogues.

Dental anxiety (DA) is the psychology obstacle of the dental patients in the dental treatment. Recently, dental anxiety becomes prevalent in the world and it greatly impacts physical health and the quality of life. The text discussed the relationship between doctors and patients in the treatment of DA

Objective To study HP infection and expression of COX-2 in gastric cancer and gastritis in order to investigate the change of COX-2 in the development of gastric cancer and the role of HP infection in the expression of COX-2.Methods A total of 180 biopsy samples from December 2004 to April 2005 were collected.We use immunohistochemistry technology to study HP infection and expression of COX-2 in gastric cancer and gastritis.Results The level of COX-2 expression in gastric cancer and atrophic gastritis complicated with dysplasia was significantly higher than in superficial gastritis(P

Objective To investigate visceral perception and gallbladder contraction function in IBS patients.Methods A total of 76 cases were selected in the First Affiliated Hospital of China Medical University which were diagnosed as IBS cases.They were divided into 2 groups(40 and 36)according to the presence of abdominal pain;they were also divided into 3 groups according to symptomatic types,18 in diarrhea group,30 in constipation and 28 in alternative diarrhea and constipation.The control group included 30 healthy volunteers.Measure the first sensation thresholds and the urge sensation of rectum water pocket volume.Use real-time supersonic image to measure the gallbladder volume before and after meal.Results Compared with normal groups,the first sensation thresholds of diarrhea-predominant IBS group was(33.00?2.02)mL vs(56.00?4.04)mL,and the urge sensation was(86.00?4.53)mL vs(135.00?3.55)mL,P

Objective To detect the normal value of the fetal middle cerebral artery peak systolic velocity (MCA-PSV) with Doppler ultrasonography, and to investigate the association between MCA-PSV and fetal anemia. Methods Doppler ultrasonography was performed in 535 normal pregnant women from the 14th to the 40th week of gestation. MCA-PSV was measured, and the standard value of the normal fetal MCA-PSV was established. Fetuses with abnormal high MCA-PSV were assessed with a standard of MCA-PSV＞mean vale of gestational weeks+2 standard deviation or 1.5 multiples of mean value of gestational weeks. Results The value of normal fetal MCA-PSV correlated well with the gestational weeks (linear regression equation was: MCA-PSV＝-9.955+1.561×weeks; r＝0.795, P<0.0001). Using 1.5 multiples of the mean of different gestational weeks to diagnose the moderate and severe fetal anemia, 7 fetuses were screened out of 209 of obstetric ultrasonography; 4 of them had MCA-PSV＞1.5 multiples of mean value of gestational weeks and were proved with induced labor. Conclusion The value of 1.5 multiples of mean value of gestational weeks of fetal MCA-PSV in prenatal ultrasonography might be an important clue for the diagnosis of fetal anemia.

Objective To investigate the clinical application and diagnostic value of impulse oscillometry lung function in children with bronchial asthma.Methods Thirty-two children (19 children in acute attack group,13 children in clinical remission group) with bronchial asthma were selected,and 20 healthy children as control group.The lung function simation by Master Screen impulse oscillometry device was detected.Results The outcome of the R5,R20,R5-R20,the negative of X5 and Fres in acute attack group (21.90±2.70) Hz]were higher than those in clinical remission group [(7.75±0.97),(3.82±0.50),outcome of the R5,R5-R20 and Fres in clinical remission group were higher than those in control group (P＜0.05),but there were no statistical significance in R20 and X5 in the two groups (P＞0.05).Conclusion Impulse oscillometry can provide various objective indicators to bronchial asthma in acute attack and clinical remission stage.which can be used as a method in detecting pulmonary function and monitoring therapy of bronchial asthmatic children.

Objective To review the clinical characteristics of adult-onset Still's disease (AOSD) patients with special pulmonary complications.Methods Medical records of 506 AOSD inpatients,who were admitted in Peking Union Medical Hospital from October 1997 through March 2015,were retrospectively reviewed.The clinical features,biochemical parameters,radiology,pulmonary function tests,ultrasound,treatments and prognosis of AOSD patients with special pulmonary complications were analyzed.Results The incidence of special pulmonary complications in AOSD inpatients was 2.2％ (11/ 506),including 3 cases of interstitial lung disease and 8 cases of pulmonary artery hypertension.All 11 patients had common manifestations of AOSD,including fever (11/11),arthralgia/arthritis (11/11),rash (9/11),lymphadenopathy and/or splenomegaly (8/11),serositis (10/11),abnormal liver function (9/ 11),neutrophilia (11/11),and hyperferritinemia (11/11).The patients with interstitial lung disease had no or mild symptom,and were diagnosed by HRCT and pulmonary function test.The patients with pulmonary artery hypertension had various symptoms including dyspnea of exertion (8/8),cough (4/8),chest suppression (2/8),and chest pain (2/8).Four patients died from severe infection,including 3 cases of pulmonary artery hypertension and 1 case of interstitial lung disease.Conclusions Interstitial lung disease and pulmonary artery hypertension are life-threatening complications of AOSD patients.Earlier recognition and prompt treatment should be made to improve patient outcomes.

Excessive and fast rise of medical expenses in China recent years has worsened the financial burden of both the government and individuals alike,making it a hotspot issue countrywide.To alleviate the difficulties of "accessibility to medical services" and "poverty or repoverty resulting from sickness" faced by the Chinese people at large,we built a multi-factor analysis model to identify key factors contributing to such a fast rise of medical expenses based on an analysis of the medical expenses in Shandong Province.In addition,we discussed the causes for GDP and headcount of medical staff in relation to the rise of medical expenses,and the strategies to curb expenses.

Objective To explore the substantial results of using comprehensive practical teaching mode and holistic nursing thinking method in the course of teaching fundamentals of nursing science,and then improve the vocational quality and ability of nursing students.Methods Randomly determined two classes as objectives: experiment class and control class.control class was adopt traditional practical teaching mode,experiment class was adopt comprehensive practical teaching mode in small community.Results Ability of standard operation,communication and knowledge were significant higher in experiment class than those of in control class.Conclusions Comprehensive practical teaching mode in small community has actual significance for strengthen the comprehendsive ability of nursing students.