1.Blistering moxibustion for 96 cases of cervical spondylosis.
Chinese Acupuncture & Moxibustion 2014;34(9):867-868
Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Moxibustion
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Spondylosis
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therapy
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Treatment Outcome
2.Exploration on‘generality and individuality’mode in clinical teaching of congenital heart disease
Tiewei LV ; Min CHENG ; Yanhong LUO ; Jihong DAI ; Leyuan JIANG ; Hong TAN ; Jie YU ; Jie TIAN ; Sujuan RAN
Chinese Journal of Medical Education Research 2012;(11):1145-1148
This study primarily explored and discussed‘generality and individuality’mode in clinical teaching of congenital heart disease(Congenital heart disease,CHD)from the aspects of teaching methods,comparison with traditional method,requirements for teachers and problems existed under present conditions.At last the paper summarized advantages and disadvantages of this model in clinical teaching of CHD and offered a new approach for clinical teaching of cardiology.
3.Cultivation and isolation of the bone marrow mesenchymal stem cells
Guo-Ping AI ; Yong-Ping SU ; Guo-He YAN ; Xing-Ze RAN ; Xiao-Hong LIU ; Cheng-Ji LUO ; Tian-Min CHENG
Journal of Third Military Medical University 2001;23(5):553-555
Objective To observe some biological features of bone marrow mesenchymal stem cells and explore the best conditions for isolatin g and culturing in vitro. Methods Common cell culture techn ique, light and electron microscopy were used to study the effects of the growth , proliferation, morphology of the bone marrow mesenchymal stem cells in differe nt adherent time, concentration of serum and cell density. Results The best culture condition in vitro for growth was 4-24 hours adherent time, 5%-10% fetal bovine serum, (4-8)×104/ml cell density. The cells were sp indle in shape and had a strong ability of proliferation. The time for cell duplication was 3 to 4 days. The cells showed the characteristics of stem cell s in electron microscope. Conclusion The best condition for iso lation and culture of bone marrow mesemchymal stem cells was successfully establ ished and some biological features were obserred. It found a base for further in vestigation and using of mesenchymal stem cells.
4.Identification of factors associated with YMDD mutation in patients with chronic hepatitis B receiving lamivudine treatment.
Xin-xian CAO ; Jia LI ; Long-min QIU ; Ya-wen LUO ; Ying-hua CHEN ; Yan RAN
Chinese Journal of Hepatology 2009;17(9):641-644
OBJECTIVETo identify factors associated with YMDD mutation in patients with chronic hepatitis B before and after lamivudine treatment in Zunyi region.
METHODS53 patients with chronic hepatitis B were enrolled in this study, HBV DNA,HBV markers, ALT, AST, TBil, albumin in the serum were examined at 0, 3, 6, 12, 18 and 24 months after lamivudine treatment. HBV genotype and YMDD mutation were determined by sequencing before lamivudine treatment. YMDD mutation was checked again if serum HBV DNA rebound to more than 1 x 10(4) copies/ml after the initial decrease.
RESULTSHBV genotype in Zunyi region is constitute of B, C and B+C genotype. YMDD mutation occurred in 18 cases after lamivudine treatment, the rate of YMDD mutation was 15.1%, and 34.0% after 1 year and 2 years treatment. There are four types of mutation: rtL180M/M204V, rtL180M/M204I, rtM204I, rtL180M. rtM204V mutation in C gene was always accompanied by rtL180M mutation (100%). The rate of rtL180M/M204V mutation in genotype C group was significantly higher than that in genotype B group (77.8% to 25.0%), the same was true for the rtL180M/ M204I mutation (22.2% to 12.5%). There was no point mutation in genotype C group. The point mutation of rtM204I and rtL180M appeared only in genotype B group. Gender, nation, family history of hepatitis B and HBeAg were not associated with YMDD mutation (P more than 0.05), while the mutation rate was associated with the disease course and severity of disease. YMDD mutation did not occur in patients with low HBV DNA level (less than 10(5) copies/ml).
CONCLUSIONYMDD mutation after lamivudine therapy is associated with HBV genotype and P gene mutation type, and prolonged treatment increases the the mutation rate. In order to reduce the incidence of YMDD mutation, patients with shorter disease course, lower HBV DNA level, more serious liver damage should be treated with lamivudine.
Adult ; Alanine Transaminase ; blood ; Antiviral Agents ; pharmacology ; therapeutic use ; Aspartate Aminotransferases ; blood ; China ; epidemiology ; DNA Mutational Analysis ; DNA Primers ; DNA, Viral ; blood ; genetics ; Drug Resistance, Viral ; Female ; Genotype ; Hepatitis B virus ; genetics ; Hepatitis B, Chronic ; blood ; drug therapy ; virology ; Humans ; Lamivudine ; pharmacology ; therapeutic use ; Male ; Mutation ; Polymerase Chain Reaction
5.Effects of Ziwuliuzhu combined selection of the source point and the collateral point on athletic injuries and the state of channels.
Bo-Ying DENG ; Jian-Fei ZHANG ; Min-Ran LUO ; Liu LIANG
Chinese Acupuncture & Moxibustion 2006;26(10):707-709
OBJECTIVETo probe into therapeutic effects of Ziwuliuzhu combined selection of the source point and the collateral point on athletic injuries and the state of channels.
METHODSNinety cases meeting diagnostic criteria were randomly divided into a group of Ziwuliuzhu combined selection of the source point and the collateral point (group A), a group of routine selection of acupoint (group B) and a group of external application of medicine (group C), 30 cases in each group. The electric conduction amount on source points of 12 channels were determined before and after treatment with a point diagnosis and treatment instrument. Changes of the state of channels and clinical therapeutic effects in the 3 groups were investigated after treatment.
RESULTSThe therapeutic effect in the group A was better than those in the other two groups (P < 0.05).
CONCLUSIONZiwuliuzhu combined selection of the source point and the collateral point has good effect on pain and other clinical symptoms of athletic injuries, and makes channels of imbalance tend to balance or recover balance.
Acupuncture Points ; Acupuncture Therapy ; Athletic Injuries ; Humans ; Medicine ; Pain
6.Metabolite Chemical Composition of the Bletilla striata (Thunb.) Reichb. f. Endophyte Penicillium oxalicum
Ran LIU ; Xuehua HAN ; Jing GAO ; Min LUO ; Dale GUO ; Guangzhi WANG
Mycobiology 2023;51(3):148-156
Penicillium oxalicum strain can be isolated from the Bletilla striata (Thunb.) Reichb. f. tubers.Its solid-state fermentation products are concentrated by percolation extraction. Separation and purification have been conducted to the ethyl acetate extracts by preparative HPLC.Based on the use of spectrometry, we have determined 17 known compounds, 12,13-dihydroxy-fumitremorgin C (1), pseurotin A (2), tyrosol (3), cyclo-(L-Pro-L-Val) (4), cis-4-hydroxy-8-O-methylmellein (5), uracil (6), cyclo-(L-Pro-L-Ala) (7), 1,2,3,4-tetrahydro-4-hydroxy-4-quinolin carboxylic acid (8), cyclo-(Gly-L-Pro) (9), 2’-deoxyuridine (10), 1-(β-D-ribofuranosyl)thymine (11), cyclo-(L-Val-Gly) (12), 2’-deoxythymidine (13), cyclo-(Gly-D-Phe) (14), cyclo-L-(4-hydroxyprolinyl)-D-leucine (15), cyclo-(L)-4-hydroxy-Pro-(L)-Phe (16), uridine (17). Here, we report compounds 1–3, 5, 7–8, 11–12, 14–17 are first found and isolated from this endophyte.
7.End-binding protein 1 stimulates paclitaxel sensitivity in breast cancer by promoting its actions toward microtubule assembly and stability.
Youguang LUO ; Dengwen LI ; Jie RAN ; Bing YAN ; Jie CHEN ; Xin DONG ; Zhu LIU ; Ruming LIU ; Jun ZHOU ; Min LIU
Protein & Cell 2014;5(6):469-479
Paclitaxel is a microtubule-targeting agent widely used for the treatment of many solid tumors. However, patients show variable sensitivity to this drug, and effective diagnostic tests predicting drug sensitivity remain to be investigated. Herein, we show that the expression of end-binding protein 1 (EB1), a regulator of microtubule dynamics involved in multiple cellular activities, in breast tumor tissues correlates with the pathological response of tumors to paclitaxel-based chemotherapy. In vitro cell proliferation assays reveal that EB1 stimulates paclitaxel sensitivity in breast cancer cell lines. Our data further demonstrate that EB1 increases the activity of paclitaxel to cause mitotic arrest and apoptosis in cancer cells. In addition, microtubule binding affinity analysis and polymerization/depolymerization assays show that EB1 enhances paclitaxel binding to microtubules and stimulates the ability of paclitaxel to promote microtubule assembly and stabilization. These findings thus reveal EB1 as a critical regulator of paclitaxel sensitivity and have important implications in breast cancer chemotherapy.
Antineoplastic Agents, Phytogenic
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pharmacology
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therapeutic use
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Apoptosis
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drug effects
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Breast Neoplasms
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drug therapy
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metabolism
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pathology
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Cell Cycle Checkpoints
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drug effects
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Cell Line, Tumor
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Female
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Humans
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MCF-7 Cells
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Microtubule-Associated Proteins
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antagonists & inhibitors
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genetics
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metabolism
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Microtubules
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chemistry
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metabolism
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Paclitaxel
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pharmacology
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therapeutic use
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RNA Interference
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RNA, Small Interfering
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metabolism
8.Phosphoregulation of the dimerization and functions of end-binding protein 1.
Jie CHEN ; Youguang LUO ; Lixin LI ; Jie RAN ; Xincheng WANG ; Siqi GAO ; Min LIU ; Dengwen LI ; Wenqing SHUI ; Jun ZHOU
Protein & Cell 2014;5(10):795-799
Adenomatous Polyposis Coli Protein
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chemistry
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metabolism
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Amino Acid Sequence
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Chromatography, High Pressure Liquid
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HeLa Cells
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Humans
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Kinesin
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chemistry
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metabolism
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Microtubule-Associated Proteins
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chemistry
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metabolism
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Microtubules
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metabolism
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Molecular Sequence Data
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Phosphopeptides
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analysis
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Phosphorylation
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Protein Multimerization
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Tandem Mass Spectrometry
9.Virological response to direct-acting antiviral therapy and changes in liver fibrosis indices in chronic hepatitis C patients with different alanine aminotransferase and aspartate aminotransferase levels in a real-world setting
Hongyu CHEN ; Qian KANG ; Hao LUO ; Ning TAN ; Jiali PAN ; Ran CHENG ; Yifan HAN ; Yuqing YANG ; Dan LIU ; Hongli XI ; Min YU ; Xiaoyuan XU
Journal of Clinical Hepatology 2021;37(2):314-317
ObjectiveTo investigate the virologic response to direct-acting antiviral (DAA) therapy and the changes in liver stiffness measurement (LSM), fibrosis-4 (FIB-4), and aspartate aminotransferase-to-platelet ratio index (APRI) after treatment in chronic hepatitis C (CHC) patients with different alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels at baseline in a real-world setting. MethodsCHC patients who attended the outpatient service of Department of Infectious Diseases, Peking University First Hospital, from December 2017 to May 2020 were enrolled, and virologic response rate was calculated. The Wilcoxon rank-sum test was used to compare LSM, FIB-4, and APRI between groups at baseline and at 12 weeks after treatment, and the chi-square test was used for comparison of categorical data between groups. ResultsA total of 48 CHC patients were enrolled, among whom 33.3% had abnormal ALT or AST at baseline. Among these patients, the virologic response rate was 85.4% at week 4 of treatment and 100% at the end of treatment and at 12, 24, and 48 weeks after treatment, and there were significant changes from baseline to 12 weeks after treatment in LSM [6.1 (51-12.4) kPa vs 8.6 (5.7-16.9) kPa, Z=-1.676, P=0.043] and APRI [0.24(0.19-0.48) vs 0.42(0.23-1.17), Z=-2.050, P=0027]. From baseline to 12 weeks after treatment, the patients with abnormal ALT or AST at baseline had significant changes in LSM [89(5.6-13.1) kPa vs 14.4(8.0-28.2) kPa, Z=-1.679, P=0.047] and APRI [0.44(0.25-0.50) vs 1.29(0.99-2.09), Z=-3.427, P=0.001]. ConclusionCHC patients achieve a high sustained virologic response rate after DAA therapy, and the patients with abnormal ALT or AST at baseline tend to have more significant improvements in LSM and APRI than those without such abnormality.
10.Experimental study on preventive and therapeutic effec ts of 5 radioprotectants on combined radiation-burn injured mice
Chao-Hua GUO ; Pei-Yan KONG ; Zhong-Min ZOU ; Yan-Hong ZHOU ; Jin-Ming ZHOU ; Yong ZHANG ; Cun-Meng SHI ; Xin-Ze RAN ; Cheng-Ji LUO
Journal of Third Military Medical University 2001;23(5):544-546
Objective To compare the irradiation-protective and inter-synergestic effects of E838,WR-2721, Rubia cordifolia, cystamin e hydrochloride and ethinyl estradiol on radiation and combined radiation-burn injury. Methods Above-mentioned drugs were given to the mice i ntraperitoneally, or intragastrcally, then, the mortality and the average surviv al d for 30 d were observed before and after the administration of the drug s. Results ①When drugs were before injury , the survival rate and the average survival d of the radiation and combined radiation-burn injured mice were increased obviously with the best effect in E838 and WR-2721. ②When drugs were given after injury, E838 and R. cordifolia also kept the effect. ③Combined appling WR-2721(pre) and E838(post)displayed a significant syner gistic reaction. Conclusion E838 and WR-2721 are more e ffective than the others in the prevention of radiation.