Objective:To investigate pharmacodynamics and safety of clobetasol propionate(CP)liposomes on guinea pigs with chronic dermatitis-eczema.Methods:Right ears of guinea pigs were repeatedly challenged with a small dose of dinitrochlorobenzene(DNCB)for the establishment of model of chronic dermatitis-eczema.The curative effect of CP liposomes was evaluated by cellular infiltrates in dermis and ear swelling thickening.Guinea pigs were given CP liposomes and CP cream on the right abdominal once a day for eight weeks,and the epidermal cell layers was calculated and compared.Results:CP liposomes could effectively reduce the number of cellular infiltrates in dermis and ear swelling thickening.This preparation for the application of eight weeks did not cause reduction of epidermal cells layers. Conclusion:CP liposomes is more effective on guinea pigs model of chronic dermatitis-eczema with less side effects.

Apolipoprotein M-sphingosine-1-phosphate axis ( apoM-S1P axis ) signaling pathway consists of apolipoproteinM (apoM), sphingosine-1-phosphate (S1P) and sphingosine-1-phosphate receptor (S1PR).Plasma apoM belongs to lipocalinsuperfamily members , and is mainly associated to high density lipoprotein( HDL), whereas HDL-cholesterol correlates inversely with cardiovascular risk .The ability of apoM to bind S1P is due to a lipophilic binding pocket within the lipocalin structure of the apoM molecule . S1P, a bioactive mediator of phospholipid metabolism , predominantly abound in HDL among all lipoproteins.S1P can not only be used as intracellular second messengers , but also as intercellular signal molecules, activating of G protein-coupled receptors (S1PR) to mediate various physiological functions.It′s clear that apoM protects human beings from atherosclerosis .Furthermore, recent studies showed that S1P has a significant impact on atherosclerosis , and ApoM-S1P axis may play a important role in the pathogenesis or progression of atherosclerosis .

Objective To observe the effect of aerosol inhalation of ambroxol combined with budesonide on neonatal pneumonia .Methods 66 infants with pneumonia treated were enrolled in this study.The patients were divided into observation group ( n =33 ) and control group ( n =33 ) according to the random number method.The treatment group were treated with ambroxol combined with budesonide and the control group were treated with budesonide.The two groups of infant lung function, clinical outcomes and blood flow were compared.Results The total effective rate (93.94%) in the observation group was significantly higher than that in the control group (45.45%), there was significant difference between the two groups (P<0.05).After treatment, FEV1 and PEF in the two groups were significantly higher than those before treatment, the observation group [(95.21 ± 7.69)%, (93.21 ±7.89)%] were significantly higher than those of the control group[(87.03 ±7.23)%, (84.55 ±7.45)%], the differences between the two groups were statistically significant (P<0.05).The OI of the two groups was significantly higher than that of the control group and the OI of the observation group was significantly higher than that of the control group.PaCO2 of the two groups decreased significantly and the PaCO2 of the observation group was significantly lower than that of the control group, the difference was statistically significant ( P<0.05 ).Conclusion The combination of ambroxol and budesonide is effective in the treatment of neonatal pneumonia.It can effectively improve the infant pulmonary function, shorten the treatment time and improve the hemodynamics of infants .

Anniversaries and Special Events ; Genomics ; Humans ; Internationality ; Mammography ; utilization ; Neoplasms ; diagnosis ; drug therapy ; genetics ; prevention & control

Objective To investigate the effect of small dose of doxepin combined with psychological behav-ior intervention in the treatment of insomnia.Methods 21 patients with insomnia were selected as the study sub-jects,took doxepin 25mg before going to bed at every night,to the research object and psychological behavior interven-tion by hand,continuous treatment for 8 weeks.Used the method of questionnaire survey before and after two periods of time,assessed by using pittsburgh Sleep Quality mder(PSQI)scale,analysed the correlation between sleep quality and sleep beliefs attitudes.Results After treatment with 8 weeks,subjected sleep quality,sleep difficulties,sleep time,sleep efficiency,sleep disturbance,hypnotic drug use,and daytime functional scores were lower than before treat-ment (t =17.34,16.65,10.54,13.37,11.65,7.66,11.57,all P <0.05 ),the difference of the score of PSQI between after treatment[(5.80 ±0.97)points]and before treatment[(13.34 ±2.28)points]was significant(t =21.27,P <0.01).Conclusion Small dose of doxipin combined with psychological behavior intervention in treating insomnia can improve the sleep quality of patients.

Bone Transplantation ; Humans ; Mandible ; transplantation ; Transplantation, Homologous

Objective To investigate the coordinate repression of angiostatin(AS)and Fas gene on human colon carcinoma LOVO.Methods Plasmid pcDNA3-AS and pcDNA3-Fas were constructed,and AS,Fas,AS and Fas gene were transfected to human colon carcinoma LOVO cells by liposome method.The expressions of target protein were detected by Western blot.The effects of transfection of AS and Fas gene on the growth of human colon carcinoma cells were detected by MTr methods.AS,Fas,AS and Fas gene were transfected to the human colon carcinoma subcutaneously implanted in nude mice by direct injection into the tumor.The tumor sizes were detected after 14 days of the first gene transfection.Results The effect of gene transfeetion in LOVO cell after twelve hours,24 h,48 h and 72 h were observed and compared with control group,co-transfection of Fas and AS gene group and Fas group significantly inhibited the growth of human colon carcinoma LOVO line cells(P＜0.01).Fourteen days after plasmid transfection,the tumor volume in group Fas and group co-transfection were signifieandy smaller than that of control,AS and Fas group(P＜0.05).The tumor volume in AS group and Fas group were significantly smaller than that of control(P＜0.05).Conclusion Angiostatin and Fas gene have coordinate repression effect on human colon carcinoma in vivo.

Acupuncture Therapy ; Attention ; Child, Preschool ; Down Syndrome ; physiopathology ; psychology ; therapy ; Humans ; Language ; Male ; Walking

Objective To explore the relationship among professional commitment, learning burnout and academic self-efficacy of undergraduate nursing students. Methods 506 undergraduate nursing students were investigated with the three Questionnaires of college students: professional commitment questionnaire, learning burnout inventory and academic self-efficacy scale. Results The scores of affective commitment, ideal commitment, normative commitment and continued commitment of the undergraduate nursing students were (28.229 ± 5.327), (20.348 ±4. 507), (19. 405 ± 3. 730), (18. 148 ± 2. 954) and( 86. 130 ± 13. 421), respectively. And the scores of depression, misconduct, low sense of accomplishment, total bumrnut of the undergraduate nursing students were(21.733 ±5.338), (18.459 ±3. 918) , (17.083 ±3. 596)and(57. 275 ±9. 766) , respectively. As well as the scores of learning ability of self-efficacy, learning behavior of self-efficacy and total self-efficacy were(35.765 ±5.835), (35.247 ±4.840)and (71.012 ±9.659), respectively. The affective commitment,ideal commitment and normative commitment of professional commitment were negatively correlated with the dimensions of learning bumout. And the continued commitment was significant correlated with the low sense of accomplishment. Otherwise, the dimensions of professional commitment were positive correlated with the dimensions of academic self-efficacy. However, the dimensions of academic self-efficacy were significant negative correlated with the dimensions of learning burnout (P＜ 0. 01). Conclusions The professional commitment, learning bumout of undergraduate nursing students are closely related with the academic self-efficacy of undergraduate nursing students. The higher the levels of professional commitment and academic self-efficacy are, the lower the level of learning bumout is.

Objective: To investigate the effect of combined acupuncture and rehabilitation on intelligence and motor development of high risk infants with perinatal brain injuries. Methods: Ninety-seven survived cases in the neonatal intensive care unit (NICU) were allocated into a treatment group of 53 cases and a control group of 44 cases following the consent of the parents. Cases in the treatment group were treated by combined acupuncture and rehabilitation; whereas cases in the control group were treated by conventional child care methods. Systemic follow-up has been made for 2 years. Then the infants' intelligence was evaluated respectively in the 6th, 12th and 34th months. Results: Compared with the control group, the mental development indexes (MDI) and physical development indexes (PDI) in the treatment group were increased by 14.96 and 9.82 respectively. There was a significant difference (X2=8.1659, P<0.01) in the abnormal intelligence rates between the treatment group (15.1%) and the control group (40.9%,). Conclusion: Combined acupuncture and rehabilitation can effectively improve the mental and physical development of high-risk infants with perinatal brain injuries. Additionally, this therapy can decrease the disability rates and increase the infants' quality of life.