Trial was made on a new way of administering drugs topically for eye conditions using a collagen corneal shield to deliver the agents. A 4mm perforating incision was made at the center of the rabbit's cornea. Thirty rabbits were used which were devided at random into 3 groups. The first group served as control, the second group was given basic fibroblastic growth factor (bFGF) as eyedrops, and the third groedup collgsu aen shields which had been soaked in bFGF. After 8 days, the maximum pressures that the corneas could stand were significantly different among the 3 groups (p

Such issues vital for the high availability of database are presented as data management,data backup and performance adjustment.With the issues classified,the solutions are also put forward.

Bone and Bones ; blood supply ; Tissue Engineering

Apolipoprotein M-sphingosine-1-phosphate axis ( apoM-S1P axis ) signaling pathway consists of apolipoproteinM (apoM), sphingosine-1-phosphate (S1P) and sphingosine-1-phosphate receptor (S1PR).Plasma apoM belongs to lipocalinsuperfamily members , and is mainly associated to high density lipoprotein( HDL), whereas HDL-cholesterol correlates inversely with cardiovascular risk .The ability of apoM to bind S1P is due to a lipophilic binding pocket within the lipocalin structure of the apoM molecule . S1P, a bioactive mediator of phospholipid metabolism , predominantly abound in HDL among all lipoproteins.S1P can not only be used as intracellular second messengers , but also as intercellular signal molecules, activating of G protein-coupled receptors (S1PR) to mediate various physiological functions.It′s clear that apoM protects human beings from atherosclerosis .Furthermore, recent studies showed that S1P has a significant impact on atherosclerosis , and ApoM-S1P axis may play a important role in the pathogenesis or progression of atherosclerosis .

AIM To explore whether or not the function of insulin like growth factor (IGF I) in promoting osteopontin gene expression and protein synthesis of rat vascular smooth muscle cell (VSMC). METHODS Cultured cells were incubated with different concentration of IGF I for different hours, then RT PCR and Western blotting analysis were used to observed the effects of IGF I on osteopontin expression of rat vascular smooth muscle cells RESULTS IGF I (20 ?g?L -1 ) could obviously induce the gene expression and protein synthesis of osteopontin, RT PCR results showed that the ratio of mRNA of osteopontin and ? actin in cells incubated with IGF I (20 ?g?L -1 ) for 3, 24 and 48 h increased by 39 70%,40 44% and 54 05% respectively, as compared with that of the control ( P

Objective: Our aim was to investigate the relationship between tumor doubling time (TD) and the prognosis of patients with lung cancer.Methods:According to the diameter of tumor or its shadow,the TD of tumor was calculated and postoperative survival time (PST) were predicted based on the Geddes' nomogram.The statistic analysis was performed after comparison with the actual survival time (AST).Results:There was no significant difference of TD among tumors of different histological types (P>0.05).The accumulated survival in patients with TD>100 days was higher than those with TD<100 days, with a significant difference (P<0.05).The AST in patients underwent surgery was markedly higher than the PST,indicating that the operation was effective.Conclusion:Tumor TD may be a valuable parameter for evaluating the prognosis and surgical treatment in patients with lung cancer.

Purpose: To evaluate the diagnostic utility of detection of Cytokeratin 19 (CK19), b-100 protein and epithelial membrane antigen ( EMA) in thyroid papillary carcinoma, follicular carcinoma and papillary hyperplasia. Methods: 65 thyroid lesions were stained immunohistochemically for CK19, S-100 protein and EMA. Results: CK19, S-100 and EMA are negative in all papillary hyperplasia. CK19 expression was strong and diffuse in 100% of papillary carcinoma including the follicular variant of papillary carcinoma, and was positive in 22% of follicular carcinoma. S-100 and EMA were respectively present in 61% and 100% of classic papillary carcinoma, in 38% and 54% of follicular variant of papillary carcinoma and in 44% and 11 % of follicular carcinoma. Conclusions: Our observations suggest that the expression of CK19, in combination with S-100 and EMA expression, is helpful in the diagnosis of thyroid lesions. CK19 is a valuable marker in the distinction of papillary carcinomas from papillary hyperplasia.

The diagnosis of post-concussion symptoms (PCS) has been a controversial issue because of that its pathogenesis is often involved in both neural damage and psychosocial factors. Recent researches have examined the diagnostic and evaluating value of several available methods or tools, including clinical interview, conventional CT and magnetic resonance imaging (MRI), neuropsychologic testing and neurochemical test. In mild traumatic brain injury (MTBI), there exist diffusive damages to white matter distributed particularly near the gray matter-white matter boundary and in the long white-matter tracts in midline structures. The conventional imaging techniques are unable to detect any positive evidence due to the diffusive nature of these injuries. This paper is to review epidemiologic study, diagnostic criteria and relevant disputes, conventional diagnostic strategy and new MRI techniques, and then evaluate the application and prospective value of them in the diagnosis and rating of PCS and MTBI. Non-invasively quantitative assessments of modern brain imaging technique may be developed to sensitive and promising tools in the diagnosis and forensic evaluation of PCS.

Objective To investigate the effect of matrix metalloproteinase-9 (MMP-9) on the prognosis of transient ischemic attack(TIA). Methods Sixty-four patients with first-onset TIA(TIA group)and 64 age and sex matched cases without any cerebrovascular disease (control group) were involved in this study. The level of MMP-9 was measured by ELISA method,and the subsequent vascular events and death rate were determined in both groups over 4 years. Results The level of MMP-9 in TIA group was significantly higher than that in control group [(5.25 ± 0.51 ) μg/L vs. (3.25 ± 0.39 ) μg/L, t = 7.830, P <0.05]. Furthermore, in TIA group, the level of MMP-9 was significantly higher in patients with primary endpoint events than that in the patients without primary endpoint events [(5.78 ± 0.48) μg/L vs. (3.15 ±0.29) μg/L, t = 3.250, P < 0.05]. Compared with the patients with lower MMP-9 level ( < 2.5 μg/L), the relative risk of primary endpoint events in higher MMP-9 level patients ( > 4.5 μg/L) was 2.612 (95% CI 1.557-5.463 ,P < 0.01 ) in TIA group. This increased relative risk for primary endpoint events in TIA group persisted after the adjustment for age, sex, body mass index, blood fat level and other cardiovascular risk factors such as hypertension, diabetes and smoking (OR =2.346,95% CI 1.418-5.216,P =0.006).Conclusions The level of MMP-9 in TIA patients has relationship with prognosis and inflammation may play an important role in TIA. MMP-9 may be a marker in predicting prognosis.

The objective of this paper is to build a real-time and highly efficient platform for medical information in wartime.The different characteristics of the wartime medical information are analyzed.Based on the hospital information system,and centering on rescue timeliness,continuity,and quality as well as the coordination of medical service command,a wartime medical information platform is designed,which is significant to the medical work in wartime.