Fusion PCR, which employs chimeric primers to generate PCR products with complementary ends in its amplifications, is a rapid and flexible method in joining different DNA fragments. This method can assemble DNA fragments without the treatment of restriction endonucleases and T4 DNA ligase. It offered a shortcut for the construction of homologous recombinant fragments. Through assembling three recombinant fragments successfully, fusion PCR procedure was improved and manipulation essentials of fusion PCR were described in detail. The results indicated that the improved procedure can assemble three or four fragments simultaneously, and the length of each fusion product is above 4.5 kb. The result recombinants were proposed to be use in further experiments, which structures had been confirmed by sequencing.

Based on a ?-tubulin gene EST sequence from Trichoderma harzianum mycelium cDNA library,a 1.74kb ?-tubulin gene coding region,together with a 1.5kb 5'-flanking region and a 1.0kb 3'-flanking region was cloned from T.harzianum by IPCR methods.The gene encodes a 446 amino acid polypeptide,which shows a high degree of homology with other fungal ?-tubulins.A three-dimensional model of the T.harzianum ?-tubulin was built based on the crystal structure of bovine tubulin using homology modeling method.Tertiary protein structure of the ?-tubulin should provide a basis for understanding a significant body of research on tubulin's properties and interactions with antimicrotubule agents.

Forkhead box Q1 (FoxQ1) is a transcription factor protein with the function of cell differentiation regulation.Recently,increasing evidence has demonstrated that FoxQ1 is significantly associated with pathogenesis of digestive system tumors.This article systematically reviewed the expression and the role of FoxQ1 in the pathogenesis of digestive system tumors.Potential therapeutic target of FoxQ1 was discussed as well.

Objective To evaluate the diagnosis and treatment of cryptorchidism and its complications,and to emphasize the appropriate surgical treatment age of cryptorchidism. Methods The data of diagnosis and treatment of 230 cases of cryptorchidism (a total of 299 undescended testicles) were reviewed.Their ages varied from 1 to 59 years and the mean age was (9.5?9.0)years.88 cases had cryptorchidism on the left side;73 cases on the right side;and 69 cases on both sides.Cryptorchidism was complicated by hypospadias in 11 cases and by latent penis in 2 cases. Results In these series only 25 cases (10.9%) underwent surgical treatment within 2 years of age.Surgical operations were performed in these cases (289 sides) and the locations of the undescended testicles were determined.Of them 273 ones underwent primary descending and fixation,15 ones underwent resection and histopathological examinations for mahgnant or shrinked tendency 1 was absent during the detection,and they were followed up for 4 months to 5 years with the success rate being 98.9 %.Of these cases 260 ones(90.0%) were external abdominal;25 ones (8.7%) were intra-abdominal;4 ones were missing (1.4%).For external abdominal testicles the positive ratio of detections by B-ultrasonography was 86.3%(126/146) and that of physical examination was 63.0% (92/146),which showed statistically significant difference ( P

This article summarizes the experience for the application of digital medical imaging devices. Such aspects have to be emphasized on as the cultivation of compound personnel for application & maintenance of digital medical imaging devices, optimization of filing, transmission and management of digital images as well as the preventive maintenance of digital imaging devices.

Maximal oxygen uptake (VO2max) is an important indicator for cardiac function. This paper reviewed the VO2max and related index applied in the physical training.

blossam.This can be used as reference for cultivating and application of Lonicara Japonica.

Abstract On October 13 2023, the Beijing Economic and Technological Development Area Center for Disease Control and Prevention recAAAAAeived a report from a school indicating an outbreak of fever among students in Class 2, Grade 4. An on-site epidemiological investigation was immediately conducted. A total of 14 cases were reported, all of whom were students, with an attack rate of 34.15%. The onset of illness was concentrated between October 6 and 15, with the main clinical symptoms being fever, cough and fatigue. There were no cases of hospitalization, severe illness or death. Seven cases tested positive for Mycoplasma pneumoniae P1-Ⅱ. Based on the on-site investigation and laboratory test results, it was an outbreak of influenza-like illness caused by Mycoplasma pneumoniae infection. The likely cause of the spread was cases infecting other classmates through respiratory droplets while attending class. The outbreak occurred only in one class and did not spread to neighboring classes. Schools should actively carry out health education to raise awareness among parents for reporting infectious diseases, and strengthen morning and afternoon screenings to improve the sensitivity of identification.

Objective: To study the effects of different temperature conditions (10, 15, 20, 25, and 30 °C) and different concentration of gibberellin (GA3) and naphthalene acetic acid (NAA) on the germination responses of Dioscorea nipponica seeds. Methods: The seed germination rate and germination vigor were recorded and analyzed statistically, through setting conditions including cold stratification and soaking treatment with different concentration of GA3/NAA in culture dishes. Results: After sand stratification at 4 °C, the germination percentage and germination vigor of D. nipponica seeds were the highest of 80.00% and 53.33% at 25 °C, respectively. After soaking in 100 mg/L GA3 for 24 h without stratification, the germination percentage and vigor of D. nipponica seeds were 66.65% and 48.35%, respectively; After soaking in 1 mg/L NAA for 12 h, the germination percentage and germination vigor were 58.35% and 3.35%, respectively. The germination process treated with low temperature sand stratification was the fastest. The seeds began to germinate at the day 6, and germinated completely within 12 d. Conclusion: The seeds should germinate at 25 °C after sand stratification at 4 °C for 60 d, or the seeds without stratification would germinate after being treated with 100 mg/L GA3.

Polypoidal choroidal vasculopathy(PCV) is a common disease of the retina,the disease is more found in colored race.In China,the mainly nosogenic population is the female about 60 years old.The pathogenesis of PCV is unknown and there is no specific medicine at present.The funduscopic present is orangered lesions,bleed and exudation.Indocyanine green angiography(ICGA) could make a definite diagnosis of PCV.In recent,the main treatment of PCV includes photodynamic therapy,intravitreal anti-VEGF therapy,surgery for treatment of complications and combined therapy.We review the treatment and diagnosis of PCV,and propose the further research direction.