Objective To investigate the therapeutic role of hyperbaric oxygen(HBO) on neonatal hearing impairment induced by hypoxic-ischemic encephalopathy(HIE),by observing the brain stem auditory evoked potential(BAEP) changes of newborn infants with HIE.Methods One hundred and six cases of HIE in newborn infants(in which 82 cases were mild and 24 cases were severe) treated with HBO and conventional therapy were chosen as HBO treatment group,while other 42 cases of HIE in newborn infants(in which 38 cases were mild and 4 cases were severe) treated with conventional therapy only were served as control group.BAEP was measured at the 3rd to 7th day on patients before treatment and 42 days after birth with or without HBO therapy respectively,and the differences of BAEP changes were compared between the both groups.Results 1.In control group,the rate of abnormality was 47.4% and 31.6%,respectively before and after therapy in mild HIE cases(P=0.240),and 75.0%,50.0% in the moderate-severe HIE cases(P=0.462).2.In HBO group,the abnormal rate was 45.1% and 25.6%,respectively in mild HIE cases before and after therapy,while 75.0% and 41.7% in moderate-severe HIE cases(Pa=0.014,0.039).3.There was obvious difference between control group and HBO group in mild HIE cases(P=0.013),while there was no significant change between the 2 groups in moderate-severe HIE cases(P=0.717).Conclusions Treatment with HBO combined with conventional therapy may improve the abnormal rate of BAEP in patients with HIE according to the present test,especially in mild HIE cases,thus earlier HBO therapy may be used to mitigate hearing impairment and improve prognosis induced by the disease.

OBJECTIVE To study infection and drug resistance of Mycoplasma from female genital tract.METHODS Mycoplasma in cervical secretion from inflammed female genital tract were identified by cultivation,and the sensitivities to antibiotics were also performed.RESULTS Mycoplasma were detected in 318 specimens among 588 patients with genital tract inflammation(54.1%),among which Ureaplasma urealyticum(Uu) accounted for 312(98.1%),and Mycoplasma hominis was 6(1.9%).The resistance to 12 kinds of antibiotics indicated that the sensitivities to 8 kinds of antibiotics were higher than 80% and the sensitivities to erythromycin,spiramycin and tetracycline were the lowest.CONCLUSIONS The infectious rates of Uu are on big rise in female genital tract with inflammation and the resistant strains increase continuously.

Actins ; metabolism ; Angiomyolipoma ; pathology ; Carcinoma, Renal Cell ; pathology ; Diagnosis, Differential ; Epithelioid Cells ; metabolism ; pathology ; Female ; Gastrointestinal Neoplasms ; pathology ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Kidney Neoplasms ; metabolism ; pathology ; Lung Neoplasms ; pathology ; Lymphangioleiomyomatosis ; pathology ; Male ; Melanoma ; pathology ; Melanoma-Specific Antigens ; metabolism ; Pancreatic Neoplasms ; pathology ; Perivascular Epithelioid Cell Neoplasms ; metabolism ; pathology ; Sarcoma, Clear Cell ; pathology ; Skin Neoplasms ; pathology ; Uterine Neoplasms ; pathology

Objective To study the distribution characteristics of TCM constitutions of hormone-dependent breast cancer patients; To provide references for TCM diagnosis and treatment.Methods TCM constitution differentiation in the 210 patients with hormone-dependent breast cancer were investigated by a questionnaire survey, which is made according to the Classification and Determination of TCM Constitutions invented by professor Wang Qi and four methods of diagnosis of TCM, with a purpose to investigate the correlation of TCM constitutions and TCM syndrome differentiation typing.Results There was statistical significance among TCM constitutions of the 210 patients with hormone-dependent breast cancer (P<0.001), with yin deficiency 43.8% (92 cases), qi stagnation 15.2% (32 cases), qi deficiency 11.4% (24 cases), blood stasis 7.6% (16 cases), and yang deficiency 6.7% (14 cases). There was statistical significance among TCM syndromes (P<0.001), with dysfunction of Chong and Ren channels 63.8% (134 cases), stagnation of liver qi syndrome 16.2% (34 cases), weakness of the spleen and stomach syndrome 11.4% (24 cases). There was statistical significance between dysfunction of Chong and Ren channels and correlation of TCM constitutions (P<0.001).Conclusion Most hormone-dependent breast cancer patients are accompanied with yin deficiency pattern and the syndrome differentiation typing of dysfunction of Chong and Ren channels. There is correlation between dysfunction of Chong and Ren channels and TCM constitutions.

Cell swelling activates or upregulates a number of anion channels. Of the volume-activated or-regulated anion channels, the volume-sensitive outwardly rectifying anion channel (VSOR) is most prominently activated and ubiquitously expressed. Volume regulated chloride channels play significant roles in a variety of physiological processes including volume regulation, proliferation, differentiation, migration,apoptosis and resistance of tumor cells. Molecular identification and activation mechanisms of VSOR are poorly understood. Many signaling molecules have been shown to regulate VSOR activity in research, playing permissive or modulatory roles. Therefore, VSOR may be a new anticancer target by identifying its signaling molecules and activation mechanisms.

BACKGROUND: Much attention has been focused on mandibular condyle fracture, which has long been considered as a severe injury to the temporomandibular joint(TMJ). Restricted by diagnostic methods, many studies have been conducted on transverse fracture whereas little attention has been paid to longitudinal fracture of the mandibular condyle. Even less is known about longitudinal fracture in young children.OBJECTIVE: To evaluate the effects of childhood longitudinal fracture of the mandibular condyle on secondary ankylosis of TMJ.DESIGN: A randomized controlled study.SETTING: Department of Stomatology, General Hospital of Chinese PLA.MATERIALS: The experiment was completed in the Center for Experimental Animals, General Hospital of Chinese PLA. Twelve young Chinese experimental miniature pigs bred by the Institute of Experimental Animals, Beijing Agriculture University, aged 2 -3 months and weighing(5 -5.5) kg, were used and raised with mixed feed, and then divided into 3 groups randomly.METHODS: Food and water was forbidden for the miniature pigs 12 hours before operation. Under anesthesia with(5.0 - 15.0) mg/kg ketamine/xylazine abdominally injected, conventional disinfection was performed in the right lateral decubitus position and layer-by-layer preauricular incisions were made on the left side of the miniature pigs. The joint cyst was incised transversely and the lower cavity of TMJ was exposed and dragged downwards. The mandibular condyle was cleaved into two vertically along the inner 1/3 part of it with a 5.0 mm-wide bone chisel to cause inferior and posterior sagittal fractures. The miniature pigs were executed 3 months and 6 months after operation, and their condyles were cut for observation.MAIN OUTCOME MEASURES:①The morphological changes of mandibular condyle specimens in the three groups observed with the naked eyes.②The pathological changes of mandibular condyle specimens under light microscope.RESULTS: The articular disc was adhered to the mandibular condyle after 3months. Temporal fossa adhered to the articular disc and mandibular condyle could still be observed in one specimen. Bifid condylar deformity was found in two specimens and no disc perforation was found. The adhesions became more severe after 6 months and bifid condylar deformity was formed in all the 3 specimens, with distinctly thickened discs. Light microscope observation:Three months after fracture, the adhesion between disc and condyle was obvious. There was no clear boundary between the surrounding tissues. The adhesion was composed of fibro-connective tissues, with a large number of chondrocytes in it. Fibro-connective tissues tightly adhered to the trabeculae were found in some adhesions. The fiber of disc became deranged and blood vessels and fatty cells were seen. Six months after fracture, blood vessels, a large number of fibroblasts and sparse chondrocytes were seen in the adhesions. The collagen fibers of the discs were arranged in whirlpool shape. There were a large number of fatty cells and blood vessels within it. The fiber-cartilage of the condylar surface was found thickened and the fiber deranged in the unadhered tissues. Proliferative changes were seen on disc surfaces.CONCLUSION: Childhood longitudinal fracture of the mandibular condyle causes severe secondary injury to TMJ, suggesting a close correlation between longitudinal fracture of the mandibular condyle and TMJ ankylosis.

Objective To optimize the extracting technology of total flavones in powder of Fagopyrum tataricum stem and leaf by enzymatic treatment. Methods The powder was treated by cellulase before extracted using water, the effects of enzyme dosage, treatment temperature, treatment time, and pH value on the extract rate of total flavones were studied. Results The optimum extracting technology was as follows: enzymatic treatment temperature: 55℃, enzyme dosage: 3.0 ?L, pH value: 6.5, treatment time: 90 min, extracting 3 times at: 90℃ for 30 min once. The extract rate of total flavones was 1.47% by this technology. Conclusion Cellulase could be applied in the assisstant extraction of total flavones in powder of F. tataricum stem and leaf.

The animal models of hypoxic-ischemic brain injury have been established inmany animals, such as monkeys, dogs, rats, mice, rabbits, and pigs. These models have provideda great deal of important information for neonatal hypoxic-ischemic brain injury. Howerver, thedifferent species vary in their susceptibility to the various types of ischemic insults. This articlereviews the animal models of hypoxic-ischemic brain injury in different species.

Organ-specific tumor cell adhesion to extracellular matrix (ECM) components and cell migration into host organs often involve integrin-mediated cellular processes. Direct integrin-mediated cell adhesion to ECM components in the space of Disse appears to be required for the successful liver metastatic formation of colon cancer. In the present study, human colon cancer HT-29 cells were transfected by liposome with integrin-beta(1) antisense oligodeoxynucleotide (ASODN). The integrin-beta(1) gene expression in HT-29 cells was significantly down-regulated. The migration of HT-29 cells was assayed using transwell cell culture chambers in vitro. The number of migrating HT-29 cells in experimental group was far less than that in control group (P<0.05). The models of hepatic metastasis in nude mice were established by the intrasplenic injection of transfected HT-29 cells. Thirty days later, the nude mice were killed and the average number of hepatic metastases (4.00+/-0.93 per mouse), average volume (10.10+/-6.50 mm(3) per mouse), average weight (0.0440+/-0.0008 g per mouse) in experimental group were remarkably reduced as compared with those in control group (P<0.05). Integrin-beta(1) expression in the hepatic metastasis was studied by immunohistochemistry (SP). Positive cell percentage of hepatic metastases in experimental group was markedly decreased as compared with that in control group (P<0.05). It was concluded that integrin-beta(1) may take part in hepatic metastasis, and down-regulation of integrin-beta(1) expression may play a key role in decreasing migration and hepatic metastasis of human colon carcinoma cells (HT-29).

Objective To establish a murine model of haploidentical peripheral blood transplantation and evaluate the characteristics of hematopoietic and immunological recovery. Methods BDF1 recipient mice conditioned with high dose total body irradiation were transplanted with neonatal peripheral blood from DBA/2 mice through tail vein.Hematopoietic recovery and graft-versus-host disease(GVHD) were observed,and the engraftment level of neutrophils and T cells were further detected by double-color flow cytometry. Results After transplantation,the levels of white blood cell(WBC),platelet(PLT) and haemoglobin(Hb) were increased significantly on day 18,21 and 18,respectively.WBC and PLT recovered within normal range on day 29 and 36,respectively,while Hb had just been partially retrieved until day 50.Long term survival was achieved in 90% of the recipient mice,and there was no evidence of GVHD.It was revealed by flow cytometry on day 50 that the proportion of donor cells in nucleated cells of recipient peripheral blood was 88.1%,including 33.92% donor neutrophils and 16.83% donor T cells. Conclusion Each newborn peripheral blood unit yields enough progenitor cells to recover the hematopoietic and immunologic system in haploidentical mice without causing GVHD.The close to full donor type chimerism was achieved,and the neutrophil was totally engrafted.