OBJECTIVE To explore the antagonistic effect of calpain inhibitor Ⅲ MDL 28170 on acute methylmercury (MeHg) neurotoxicity. METHODS The rats were randomly divided into MDL 28170 (50 mg·kg~(-1),ip) control group, MeHg (10 mg·kg~(-1),ig), and MeHg (10 mg·kg~(-1),ig)+MDL 28170 (50 mg·kg~(-1), ip) group. The escape latencies were observed by Morris water maze test. The neurons of positive μ-calpain were determined by immunohistochemical method, the expression of μ-calpain was analyzed by Western blotting, apoptotic neurons were observed by TUNEL method, and microtubule-associated protein 2 (MAP2) expression was detected in neuron by immunohistochemical method. RESULTS MeHg was ig given for 3-7 d, the initial signs of behavioral changes developed in MeHg group, the escape latencies were lengthened in Morris water maze test (P<0.01). The expression and activity of μ-calpain elevated obviously in parietal cerebral neurons(P<0.01), neuronal apoptosis was markedly increased(P<0.01), and the decomposition of MAP2 was reduced in cortical neurons obviously. And the above changes were suppressed obviously by treatment of MDL 28170. CONCLUSION μ-Calpain may correlate with neuronal apoptosis induced by MeHg, MDL 28170 markedly reduces neuronal apoptosis, which might act as therapeutical method for MeHg poison.

Aim To explore the protective effect of SMT on acute cerebral infarction.Methods Focal cerebral infarction was induced by photochemistry.The expression of iNOS was detected in microglia by double fluorescent immunohistochemistry and laser scanning confocal microscopy. NO content at post-ischemic time points was determined by electron spin resonance(ESR) technique and standard addition method.TTC stain was used to determine the infarct volume.Results At ischemic early period,compared with control group,NO content was not remarkably different after 2 h,6 h of ischemia in the treatment groups,infarct volumes also were not obviously changed.At ischemia mid and late period 12 h,24 h,48 h,the expression of iNOS and NO content was suppressed by SMT treatment in microglia.In TTC stain results,infarct volumes were increased obviously after 12 h,and achieved the peak after 24 h,48 h in control groups,infarct volumes in treatment groups were significantly decreased after 12 h,24 h,48 h.Conclusion SMT may play a protective role in acute cerebral infarction by reducing the expression of iNOS and NO content in microglia.

AIM:To explore the regulating mechanism of microglia transplantation in treating cerebral infarction.METHODS:Focal cerebral infarction model was established by photochemistry. The histological and immunohistochemical methods were used to observe the effects of microglia transplantation on cerebral infarction. The expressions of nerve growth factor(NGF) and interleukin-10(IL-10) were detected by double fluorescent immunohistochemistry and Western blotting.RESULTS:Microglia marked with green fluorescence was observed in ischemic penumbra,indicating that microglia can be transplanted across the blood-brain barrier. Infarct volumes and death number of cells were significantly reduced compared to non-transplantation animals. The expressions of NGF and IL-10 were markedly increased in microglia in transplantation group.CONCLUSION:Microglia can be transplanted across the blood-brain barrier. The cells have protective effects in ischemic penumbra by secreting NGF and IL-10,which might serve as therapeutical method for treating cerebral infarction.

Bone and Bones ; blood supply ; Tissue Engineering

Adult ; Carcinoma, Endometrioid ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Hysterectomy ; Ovarian Neoplasms ; pathology ; surgery ; Sex Cord-Gonadal Stromal Tumors ; pathology ; surgery

Aged, 80 and over ; Antigens, CD ; metabolism ; Antigens, Differentiation, Myelomonocytic ; metabolism ; Diagnosis, Differential ; Fasciitis ; pathology ; Fibroblasts ; pathology ; Fibrosarcoma ; metabolism ; pathology ; surgery ; Forearm ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Male ; Myxosarcoma ; metabolism ; pathology ; surgery ; Soft Tissue Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism ; alpha 1-Antichymotrypsin ; metabolism

Histiocytoma, Benign Fibrous ; Humans ; Male ; Middle Aged ; Orbital Neoplasms

Female ; Genital Neoplasms, Female ; classification ; pathology ; Humans ; Ovarian Neoplasms ; pathology ; Papillomavirus Infections ; Precancerous Conditions ; pathology ; Uterine Cervical Neoplasms ; pathology ; virology ; World Health Organization

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The academic connotation of "pathogenic factors" is discussed in this article from three aspects of etiology,diagnosis and treatment.It is considered that endogenous or exogenous pathogenic factors oppose to and destruct healthy energy.TCM pathogenic factors,obtained from "examining syndromes for causes of diseases",are diverged from physical,chemical and biological those in modern medicine.What is meant by "therapy of expelling pathogenic factors" is not a single method of dispelling toxin and killing germs,but the resort of regulating the integrated abilities of the human body fighting against pathogenic factors.