1.Proposal for standardized authors' name citing in original plant Latin name listed in the Chinese Pharmacopoeia.
China Journal of Chinese Materia Medica 2014;39(9):1743-1748
In 2010, Chinese Pharmacopoeia Committee officially enacted Chinese Pharmacopoeia (2010 edition). The Volume 1 of the pharmacopoeia is comprised of the medicinal materials and the decoction pieces, the essential oils and extracts of medicinal plants, prescription preparations and single preparation, etc., which not only provides Latin names of Chinese medicinal materials, also provided Latin names of the original medicinal plants to effectively control the quality of Chinese medicinal materials. In order to raise awareness of correctly citation and maintain the authority and standardization of Chinese Pharmacopoeia, this paper briefly describes abbreviations rules of authors' name of plant scientific name according to the 'International Code of Botanical Nomenclature, ICBN'. Through comparing with the rules of ICBN, 'Flora of China' (Chinese edition and English edition), and authority international plant catalogue databases, the authors made statistic and analysis of the non-standard cited authors' names phenomena of the original plant scientific names recorded in the Chinese Pharmacopoeia (2010 edition), and the revision suggestions are proposed.
Asian Continental Ancestry Group
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Authorship
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China
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Databases, Factual
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Drugs, Chinese Herbal
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classification
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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methods
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Pharmacopoeias as Topic
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standards
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Plants, Medicinal
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chemistry
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classification
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Reference Standards
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Terminology as Topic
3.Herbal textural research on species of Xanthii Fructus.
Dong-mei XIE ; Min-jian QIN ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(9):1842-1844
Xanthii Fructus is a traditional medicine for the treatment of nasal diseases in clinic, mainly come from the burs of Xanthium sibiricum with a worldwide distribution. By sorting and studying literature of Chinese medicine and comparing different figures recorded with the morphological description of several species from Xanthium (Asteraceae) in the Flora of China, combining the biological investigation in resource survey, the article pointed out that the burs or the whole herbs of X. mongolicum, as well as X. sibiricum, has been used by the traditional Chinese medicine in ancient time. It provides a reference for further studies in the future.
China
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Herbal Medicine
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history
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History, Ancient
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Medicine in Literature
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Medicine, Chinese Traditional
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history
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Xanthium
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anatomy & histology
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classification
5.Fermentation of Bacillus subtilis ge25 strain and preliminary study on its antagonistic substances.
Chen-Yun HU ; Yong LI ; Min LIU ; Wan-Long DING ; Min-Jian QIN
China Journal of Chinese Materia Medica 2014;39(14):2624-2628
Panax ginseng is one of the most important traditional Chinese herbal medicine, soil borne diseases influenced the yield and quality severely. In our previous work, endophytic Bacillus subtilis ge25 strain was isolated from ginseng root, and which showed significant antagonistic activity against several most destructive ginseng phytopathogens. In the present work, crude protein and lipopeptid extracts were prepared from LB and Landy supernate by salting out, acid precipitation methods respectively. The antagonistic activity of crude extracts and stability to temperature and protease digestion were examined by ginseng phytopathogen Alternaria panax. Results showed that, the antagonistic activity of crude protein extracts from LB culture was complete and partially lost when treated by high temperature and proteinase K. However, crude lipopeptid from Landy culture showed significant stabile antagonistic activity to them. Acid-hydrolyzation and TLC-bioautography analysis showed, that the crude lipopeptide contained at least one cyclic lipopeptide. In consideration of the stability and perfect antagonistic activity of ge25, further researches will promote the biocontrol of ginseng diseases in the field.
Alternaria
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drug effects
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physiology
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Bacillus subtilis
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metabolism
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physiology
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Bacterial Proteins
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isolation & purification
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metabolism
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pharmacology
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Endopeptidase K
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metabolism
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Endophytes
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metabolism
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physiology
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Fermentation
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Lipopeptides
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isolation & purification
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pharmacology
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Panax
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microbiology
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Plant Roots
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microbiology
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Temperature
6.Change of ?-amyloid precursor protein processing in platelet of Alzheimer's disease patients
Xiao-Qin HUANG ; Jian-Ping JIA ; Chun-Qiu FAN ; Xiu-Min DONG
Chinese Journal of Neurology 1999;0(06):-
Objective To investigate the characteristic of ?-amyloid precursor protein (A?) processing in activated platelet in AD.Methods Thirty-six sporadic AD patients and 30 control subjects were included in this study.Blood was collected from the subjects to separate platelets.After treated by thrombin,the soluble amyloid precursor protein (APP) level in the snpernatants of platelets from 36 were analyzed by means of western blot with a specific antibody recognizing soluble APP.Meanwhile A? level was measured by radioimmunoassay.Results After treated with thrombin,the level of soluble APP in the supernatants of platelets in patients with AD decreased by 31.0% (P
7.Clinical significance of detecting serum CYFRA21-1,VEGF and PDGF in patients with non-small cell lung cancer
Yan DU ; Jing-Min ZHOU ; Jian-Wen QIN ; Shu-Ping MA ; Dong-Sheng SHI ;
Cancer Research and Clinic 2006;0(12):-
Objective To explore the diagnosis and TNM stage effect of serum CYFRA21-1,VEGF and PDGF in patients with non-small cell lung cancer.Methods The electrochemiluminescence immunoas- say was used to detect serum CYFRA21-1,and the sandwich enzyme-linked immunoabsorbentassay(ELISA) was used to detect serum VEGF and PDGF in patients with non-small cell lung cancer and 30 normal healthy controls.Results Compared with healthy control group,the level of serum CYFRA21-1,VEGF and PDGF in non-small cell lung cancer group were much higher(P0.05).The serum CYFRA21-1 level was positively correlated with VEGF and PDGF(P
8.Comparison of abdominal CT and pathological findings in chronic schistosomiasis
Tie LIU ; Min-Fang SONG ; Ji-Shun DONG ; Jian HE ; Ke-Qin ZHU ; Hai-Feng QIAN ;
Chinese Journal of Radiology 2000;0(11):-
Objective To retrospectivel y analyze the abdominal CT findings and pathological results of the chronic schist osomiasis so as to improve the diagnostic accuracy of the disease. M ethods The plain abdominal CT scanning was performed in 103 cases an d enhanced CT scanning in 81 cases. The pathological specimen which was consist ent with the section of CT scan was obtained in each cases. Results On CT scanning, liver cirrhosis was seen in 84 cases, various calci fication in liver in 71 cases, liver cancer in 12 cases, enlargement of sple en in 78 cases, calcification in spleen in 13 cases, wall-thickening in colon i n 27 cases, calcification in colon in 31 cases, and colon cancer in 9 cases. Pa thological examination revealed various fibrosis and formation of pseudolobule. The eggs and calcification could be seen in pseudolobule and septa, colonic sub mucosa, and regional lymph nodes. Fibrous hyperplasia in colonic wall and hyper plasia in mucous membrane were obvious. Fibrous hyperplasia and calcification w ere seen in spleen, but the eggs were not found. Conclusion The liver and colon are the major organs affected by chronic schistosomias is in abdomen, and the CT findings are obvious too. The pathological features o f spleen are accompanied with liver cirrhosis. CT is the important imaging meth od in diagnosing chronic schistosomiasis and pathological changes.
9.Effect of andrographolide on the mRNA expression of MexAB-OprM efflux pump of Pseudomonas aeruginosa PAOI strain
Hong-Tao LI ; Chun-Ming WU ; Hui-Min QIN ; Guo-Jun LI ; Jian-Xin SONG ;
Chinese Journal of Infectious Diseases 2001;0(06):-
Objective To develop a real-time polymerase chain reaction(PCR)system to determine transcriptional level of MexAB-OprM multidrug efflux pump gene and to investigate the impact of androgra- pholide on MexAB-OprM gene transcription in Pseudomonas aeruginosa.Methods The fragments of mexB gene of mexAB-oprM operon and 30S rRNA gene rpsL were amplified and cloned into two plas- mids respectively.These plasmids were used as external standards for real-time PCR.Real-time PCR was applied to measure the mRNA transcripition of mexB and rpsL gene in Pseudomonas aeruginosa growing in medium with different concentrations of andrographolide.Results The plasmids for standard curve were constructed successfully.The relative mexB mRNA expressions in 50,100,150 and 200?g/mL andrographolide were 0.04?0.03,0.06?0.07,0.09?0.03 and 0.04?0.03 respectively, which were significantly lower than that in the control(0.24?0.04,P0.05).Conclusion Andrographolide can reduce the transcriptional level of MexAB-OprM,which may he one mechanism for its anti-infection effect.
10.Preliminary evaluation on the detection of serum level of antibody to Bacillus anthracis by enzyme-linked immunosorbent assay using crude antigen
Jian-chun, WEI ; Hui-juan, ZHANG ; Feng-qin, MA ; En-min, ZHANG ; Dong-zheng, YU
Chinese Journal of Endemiology 2008;27(3):329-331
Objective To evaluate the method of detecting antibodies to Bacillus anthracis by enzymelinked immunosorbent assay(ELISA)using crude antigen.Methods The anti-Bacillus anthracis antibody levels in sera of 42 healthy people and 42 patients were detected by indirect ELISA.Standard curve was plotted using the data from positive controls,based on which the relative content of each serum was calculated and compared with the result of rLF.Results The median of antibody's relative content in patient group and healthy people group are 1.19 and 0.24,the differences being statistically significant(uc=7.643,P<0.05).The result of crude antigen is in concordance with rLF(but not parallel absolutely).Conclusions Crude antigen can distinguish most of patients with healthy population effectively.The results suggested that crude antigen is applicable in anti-Bacillus anthracis antibody surveillance.