GH concentration in plasma decline with age. GH and GH response to GHRH are influenced by sex hormones, thereby changing around the menopause. In several aspects, features of aging resemble those of a state of GH deficiency. It has been argued the declining GH function, along with other factors, might be a causal factor in osteoporosis. The purpose of this study was to investigate that postmenopausal osteoporosis in ovariectomized rat could be prevented by rhGH. Fifty-four Sprague-Dawley rats(weight 140-200g) were devided 3 groups. Group 1(n=18) was sham operation; Group 2(n=18) was ovariectomized and received subcutaneous injection with 0.05 cc normal saline; Group 3(n=18) was ovariectomized and received subcutaneous injection with 0.2 IU rhGH. Group 2 and 3 were injected daily, 6 day per week. Each group was devided three subgroups(n=6) and were sacrificed at 6 week, 10 week, 14 week, respectively. Group 2 showed a significant increase in body weight, femur length, serum IGF-1 level, serum PICP and ICTP level at 6 weeks, 10 weeks, 14 weeks and a significant decrease in ash weight of tibia, width of bony spicules, at 14 weeks than Group 1. Group 3 demonstrated a significant increase in body weight, femur length, serum IGF-1, serum PICP and ITCP levels and a insignificant decrease in ash weight of tibia and width of bony spicules, at 6 weeks, 10 weeks, 14 weeks than Group 1. At 14 weeks, Group 3 showed a significant increase in serum IGF-1, Serum PTH, Serum PICP. From these data, we conclude that although rhGH administration leads to an activation of bone turnover and more stimulation of bone formation, it does not prevent a bone loss in ovariectomized rat.
Aging ; Animals ; Body Weight ; Female ; Femur ; Gonadal Steroid Hormones ; Humans* ; Injections, Subcutaneous ; Insulin-Like Growth Factor I ; Menopause ; Osteogenesis ; Osteoporosis* ; Osteoporosis, Postmenopausal ; Ovariectomy ; Plasma ; Rats* ; Rats, Sprague-Dawley ; Tibia

In a population-based study with 4 years of follow up, we evaluated the prevalence of Coxiella burnetii in cattle on Ulleung Island, Korea. In this study, the rates of C. burnetii infection in cattle on Ulleung Island were determined by PCR and were found to be 0.3–1.0% in the period 2011–2014. All 17 C. burnetii partial 16S rRNA gene sequences from PCR-positive cattle were identical and 2 geographic representatives were included in our analysis. The nucleotide sequences of the 2 samples showed high (98.4–100%) identity with C. burnetii sequences obtained from the GenBank. In this long-term tracking study, the number of cattle positive for C. burnetii on Ulleung Island was low. To prevent the transmission of C. burnetii on Ulleung Island, control strategy should include biosecurity improvement in surveillance, livestock management, administering suitable tests before purchasing animals to detect C. burnetii shedders, and restricting movements between herds.
Animals ; Base Sequence ; Cattle ; Coxiella burnetii ; Coxiella ; Databases, Nucleic Acid ; Follow-Up Studies ; Genes, rRNA ; Korea ; Livestock ; Phylogeny ; Polymerase Chain Reaction ; Prevalence

We identified the presence of Toxoplasma gondii in ticks and their host dogs, and assess the potential role of ticks as reservoirs for this pathogen. A total of 1,230 feeding ticks were collected from 340 dogs. The tick species identified included Haemaphysalis longicornis, H. flava, and Ixodes nipponensis. Detection of the T. gondii B1 gene occurred in 2 dogs (0.6%) and 4 tick pools (0.9%). Genotyping confirmed the presence of the I/III genotype. This study is the first to report the molecular detection of T. gondii in both canine ticks and their hosts. Our findings offer important insights into the dynamics of T. gondii transmission between vectors and their hosts.

We identified the presence of Toxoplasma gondii in ticks and their host dogs, and assess the potential role of ticks as reservoirs for this pathogen. A total of 1,230 feeding ticks were collected from 340 dogs. The tick species identified included Haemaphysalis longicornis, H. flava, and Ixodes nipponensis. Detection of the T. gondii B1 gene occurred in 2 dogs (0.6%) and 4 tick pools (0.9%). Genotyping confirmed the presence of the I/III genotype. This study is the first to report the molecular detection of T. gondii in both canine ticks and their hosts. Our findings offer important insights into the dynamics of T. gondii transmission between vectors and their hosts.

We identified the presence of Toxoplasma gondii in ticks and their host dogs, and assess the potential role of ticks as reservoirs for this pathogen. A total of 1,230 feeding ticks were collected from 340 dogs. The tick species identified included Haemaphysalis longicornis, H. flava, and Ixodes nipponensis. Detection of the T. gondii B1 gene occurred in 2 dogs (0.6%) and 4 tick pools (0.9%). Genotyping confirmed the presence of the I/III genotype. This study is the first to report the molecular detection of T. gondii in both canine ticks and their hosts. Our findings offer important insights into the dynamics of T. gondii transmission between vectors and their hosts.

We identified the presence of Toxoplasma gondii in ticks and their host dogs, and assess the potential role of ticks as reservoirs for this pathogen. A total of 1,230 feeding ticks were collected from 340 dogs. The tick species identified included Haemaphysalis longicornis, H. flava, and Ixodes nipponensis. Detection of the T. gondii B1 gene occurred in 2 dogs (0.6%) and 4 tick pools (0.9%). Genotyping confirmed the presence of the I/III genotype. This study is the first to report the molecular detection of T. gondii in both canine ticks and their hosts. Our findings offer important insights into the dynamics of T. gondii transmission between vectors and their hosts.

We identified the presence of Toxoplasma gondii in ticks and their host dogs, and assess the potential role of ticks as reservoirs for this pathogen. A total of 1,230 feeding ticks were collected from 340 dogs. The tick species identified included Haemaphysalis longicornis, H. flava, and Ixodes nipponensis. Detection of the T. gondii B1 gene occurred in 2 dogs (0.6%) and 4 tick pools (0.9%). Genotyping confirmed the presence of the I/III genotype. This study is the first to report the molecular detection of T. gondii in both canine ticks and their hosts. Our findings offer important insights into the dynamics of T. gondii transmission between vectors and their hosts.

Toxoplasma gondii, a common protozoan parasite, poses significant public health risks due to its potential to cause toxoplasmosis in humans and can be contracted from pigs, which are considered its critical intermediate host. The aim of this study is to evaluate the prevalence of T. gondii in slaughtered pigs for human consumption, emphasizing the zoonotic implications and the need for improved biosecurity and monitoring practices in pig farming. A total of 1,526 pig samples (1,051 whole blood samples and 384 lung tissue samples from the local slaughterhouse and 91 aborted fetus samples from local farms) were collected throughout the whole country of Korea in 2020. Among them, 6 (0.4%) were found to be infected with T. gondii by nested PCR. When compared by sample type, the prevalence of T. gondii was significantly higher in the aborted fetus samples (2.2%, 2/91) than in the blood (0.3%, 3/1,051) and lung tissue samples (0.3%, 1/384). The B1 gene sequence of T. gondii was similar (97.9–99.8%) to that of the other T. gondii isolates. This study represents the first molecular genotyping survey of T. gondii in the lung tissue of fattening pigs and aborted fetuses in Korea. Our findings indicated the importance of adopting preventive measures including the implementation of rigorous farm hygiene protocols and the promotion of public awareness about the risks of consuming undercooked pork. By addressing the gaps in current control strategies and encouraging the One Health approach, this study contributes to the development of more effective strategies to mitigate the transmission of T. gondii from pigs to humans, ultimately safeguarding public health.

OBJECTIVE: To evaluate the usefulness of alendronate for prevention and treatment of postmenopausal osteoporosis this study was taken. METHODS: This prospective randomized clinical trial examined the effects of oral alendronate and HRT(conjugated estrogen plus medroxyprogesterone acetate), in combination and seperately, on BMD, biochemical markers of bone turnover in 79 women with low bone mass. Treatment included alendronate(10mg daily) plus HRT(group I, n=38), or HRT(group II, n=41) for 6 months. Bone density measurements were performed at months 0 and 6 at the lumbar spine. Biochemical markers of bone turnover were also measured every three months. RESULTS: Serum Osteocalcin decreased by 19.2% in group I and by 10.0% in group II at 3 months(p<0.05), and by 30.9% in group I and by 19.8% in group II at 6 months(p<0.05). Urinary deoxypyridinoline showed decrease of 19.75%(I) vs. 10.4%(II) at 3 months, 30.1%(I) vs. 20.7%(II) at 6 months, the difference was significant. Percent change of BMD measurements from baseline at 6 months in group I was 6.2% and in group II 0.6% on the lumbar spine(p<0.05). CONCLUSION: The treatment with alendronate is useful to postmenopausal women with osteoporosis by decreasing bone turnover markers, and by increasing the BMD.
Alendronate* ; Biomarkers ; Bone Density ; Estrogens ; Female ; Humans ; Medroxyprogesterone ; Osteocalcin ; Osteoporosis* ; Osteoporosis, Postmenopausal ; Prospective Studies ; Spine

The analysis of regional myocardial function is important for the evaluation of myocardial performance. We evaluated whether the motion images of the coronary bifurcation points can be used for computation of the regional deformation and the regional wall stress. In 5 mongrel dogs, 4 to 7 lead markers were implanted adjacent to the bifurcarion points of coronary artery. Left ventricular pressure curve was obtained, and left ventriculography was performed to measure the major and minor axes of the left ventricle. Afterthen, biplane coronary arteriography was performed. The motion images of the lead markers and the corresponding coronary bifurcation points were used as landmarkers for the kinetic analysis of the regional wall deformation and wall stress. The results of the analysis using two kinds of landmarkers were well correlated(P<0.001). Analysis of the diagnosis biplane coronary cineangiograms of each patient group demonstrated that quite difference wave forms between the normally perfused segment and the underperfused segments. In group I there was no coronary arterial narrowings and no regional wall motion abnormalities. Their mean and peak wall thicking were 10.10+/-9.28% and 47.99+/-20.70%. And their peak and mean values of circumferential and longitudinal wall stresses are as follows; 2.70+/-0.74x10(5)dyn/cm2, 2.34+/-0.71x10(5)dyn/cm2, 0.84+/-0.21x10(5)dyn/cm2, 2.34+/-0.71x10(5)dyn/cm2, 0.84+/-0.21x10(5)dyn/cm2 and 0.64+/-0.13x10(5)dyn/cm2. Of 5 male patients having coronary arterial disease with regional wall motion abnotmalities(Group III), 2patients have single vessel disease, 2 paitents have 2 vessel disease. In subgroup b of group III, there was a significant decrease of regional wall thickening(mean and peak value; 0.54+/-9.91%.14.87+/-12.055; P<0.001 vs. group I), and increasing tendency of regional wall stresses. Using biplane coronary cineangiogram, which is routine diagnostic procedure in coronary artery disease, this method can valuate regional myocardial function. And this method can evaluate regional myocardial function. And this method will be especially useful, when serial coronary cineangiograms are needed for serial evaluation of patients, such as before and after percutaneous transluminal coronary angioplasty, coronary arterial bypass graft surgery, etc.
Angiography ; Angioplasty, Balloon, Coronary ; Animals ; Coronary Artery Disease ; Coronary Vessels ; Diagnosis ; Dogs ; Heart Ventricles ; Humans ; Male ; Transplants ; Ventricular Pressure