Corydalis amabilis Migo total alkaloid (COAMTA) given in vitro or in vivo caused an inhibition on ADP-induced blood platelet aggregation. In vitro, COAMTA 15.6, 31.3 & 62.5 ?g/ml PRP showed their inhibitory rates of 8.2, 32.0, & 36.8%. In vivo, at 10, 20, 40 min after iv COAMTA ( 0.6 mg/kg ) , the rates of inhibition were 40, 62.8, 24%.The thrombi formed in vitro were measured also. The results showed that COAMTA given in vitro or in vivo reduced the length and weight of thrombus, at same time, reduced rat platelet adhesion.A carotid-jugular extracorporeal shunt was made in the rats. A 6cm length of silk thread was placed in the shunt. 15 min after the restoration of blood flow in the shunt, the Wet weight of thrombus developed on the thread was measured.The thrombus weight was significantly reduced when COAMTA was given iv 0.3, & 0.6 mg/kg, im 0.9 mg/kg & po 1.5 mg/kg. The thrombus weight of control group was 26.1?0.7, but that of the medicated group was 8.9?1.7, 18.0?1.3, 16.4?0,6 a & 19.7?0.7 mg. The inhibitory rates were 66.1, 31.3, 37.2, & 28.4%.

Corydalis amabilis Migo total alkaloids (COAMTA) possessed antihypertensive and vaso-dilatative action in animal experiments. Its effects on the physiological properties in isolated guinea pig papillary muscles & atrium preparations were described in this papar.10mg/L COAMTA markedly prolonged the functional refractory period(FRP) and inhibited epinephrine induced automaticity. Suppression of normal & abnormal automaticity and prolongation of FRP may contribute to the effectiveness of COAMTA on arrhythmia.

Objective:To estimate the prevalence of antiretroviral drug resistance in treatment-naive in-dividuals with human immunodeficiency virus ( HIV ) in China. Methods: Five electronic databases [ Chinese BioMedical Literature Database ( CBM) , Chinese Journal Full-text Database ( CNKI) , Chinese Science-Technology Journal Database ( VIP) , Wanfang Data, and PubMed] were searched for studies of HIV drug resistance in untreated individuals. Drug resistance data were abstracted then pooled using the random effect model. Subgroup analysis was done across sampling time, location, study population ( mean age and infection status) , and sample size. Results: Seventy-six studies were included for our meta-analysis (46 in Chinese, 30 in English). The pooled rates of drug resistance to total, to non-nucleoside reverse transcriptase inhibitor ( NNRTI ) , to nucleoside reverse transcriptase inhibitor (NRTI), and to protease inhibitor ( PI) were 4. 7% (95%CI:4. 0% -5. 4%), 2. 3% (95%CI:1. 8% -2. 8%), 1. 8% (95%CI:1. 3% -2. 3%), and 1. 4% (95%CI:1. 1% -1. 8%), respective-ly. All the rates before 2007 were higher than those for 2008 or later. Meanwhile, significant differences were found in the sample areas (P <0. 05), in which, the rates in South-central and Southwest were both higher than 5%. The difference was complex between mean age and infection status subgroup, and we found the total prevalence in the group under 25 years and the newly infected, and confirmed group was lower than in the others. For sample size, all the rates in the group under 100 samples were higher than in the others, and the difference was significant (P<0. 05). Conclusion: The prevalence of HIV primary drug resistance in China was 4. 7%, which stayed low, but was also close to the line set by WHO. Enhanced surveillance for drug resistance is necessary in high epidemic areas including the South-central and Southwest China whose prevalence has crossed the line.

Genetic Variation ; Humans ; Ticlopidine ; analogs & derivatives ; pharmacokinetics ; pharmacology

Objective To investigate the features of clinical pathology,phenotye and differential diagnosis of thyroid follicular carcinoma-like renal tumor.Methods A 25-year-old female patient was hospitalized in February 28,2011 because of hypertension for 20 months.Physical examination revealed a right renal space-occupying lesions without percussion pain.Blood pressure was 200/130 mm Hg (1 mm Hg =0.133 kPa).Thyroid,heart,lung,and abdominal examination showed no abnormalities.Blood,urine routine,biochemical examination,blood catecholamines,and thyroid function test results were normal.CT examination showed that a visible circular nodules with clear boundary was in the upper pole of the right kidney,and the maximum section about 2.2 cm × 3.0 cm withuneven density andrenal artery was pressed down.Clinical diagnosis was right renal tumor,and right renal tumor was removed under general anesthesia.Intraoperative tumor was located in the right renal hilumwithclear boundary,and the surrounding mild adhesions.Postoperative blood pressure wasnormal.Results General inspection of the specimen:a 2.5 cm diametermasswith intact capsule.The color of the section of the mass is gray red and gray.Multiple small cysts are seen in the section and the cysts contain jelly material.Microscope inspection:the tumor tissue is arranged like thyroid follicular and the follicular contains much acidophil colloid.Immunohistochemistry:the tumor cell is EMA (+),Vimtin (+),CK7 (+),CK20 (+),CD117 (-),TTF-1 (-),TG (-),CgA (-),Syn (-).Conclusions Thyroid follicular carcinoma-like renal tumors is a very rare kidney epithelial cell tumor,which is attributable to any known subtypes of renal cell carcinoma.To improve the understanding of it can avoid misdiagnosis and prevent inappropriate treatment.

Objetive To study the effect of Paeonol on PGI,TXA2,ET and NO in diabetic rats.Methods Streptozocin was used in dosage of 60 mg/kg on rats to make diabetic animal model.Defferent dosages of Paeonol were used on diabetic animal models.6-Keto-PGF1?,TXB2,ET and NO were tested after 30 days. Results Compared to the control group,6-Keto-PGF1?(pg/mL)of Paeonol groups increased from 89.75? 2.75,89.97?7.28,89.97?11.36 to 120.03?13.85,108.34?11.25,105.32?8.85 respectively;TXB2 (pg/mL)decreased from 157.64?10.36,156.64?11.35,153.33?19.40 to 124.46?18.67,136.40?18.15, 138.40?22.20 respectively;ET(pg/mL)decreased from 181.68?5.10,181.27?4.76,181.04?4.19 to 140.55?3.01,150.51?2.22,161.02?3.76.The change of 6-Keto-PGF1?,TXB2 and ET was related to the dosage of Paeonol.NO has no significant change.Conclusions Paeonol can decrease the ET and TXB2 in diabetic rats,and increase 6-Keto-PGF1?in diabetic rats.

Objective:To analyze the results of syphilis specific serological tests among entry personnel in Beijing port during year 201 2 -201 4 in order to provide evidence for the prevention and control of syphilis.Methods:Demographics data and syphilis specific test results of entry personnel were obtained from the information system of Beijing International Travel Healthcare Center and input into Excel 201 6. Database for entry personnel was built up accordingly and was analyzed by IBMSPSS22.0.Results:The number of entry personnel in Beijing port during year 201 2 -201 4 was 1 06 302 person-time.According to the evaluation criteria,71 308 persons were recruited and analyzed,of whom 277 were tested serologi-cally positive and the total prevalence was 0.39% (95% CI:0.34% -0.43%).The prevalence was highest in the persons aged 40 -49 years (0.81 %)and was higher in males (0.50%)than in females (0.23%).The persons from Africa held higher prevalence (1 .27%)than from other five regions.As multivariate logical regression analysis indicated that the risk of syphilis infection was 1 0.38 times more in the persons aged 40 -49 years than in the persons <20 years (95%CI:5.59 -1 9.28),and 1 .85 times more in males than in females (95%CI:1 .40 -2.44).For the people from Africa,South America and Asia,the infection risks were respectively 4.89 times (95%CI:2.97 -8.06),4.82 times (95%CI:2.39 -9.74)and 1 .72 times (95%CI:1 .1 5 -2.59)more than that for the people from North Ameri-ca.Rising trend of the prevalence was observed in both genders by age groups (P value for trend χ2 <0.05).Except for Asia,the prevalences of the people from other five regions were rising slightly by years,however there were no obvious trends (P value for trend χ2 >0.05).Twenty-seven seropositive cases were found within 6 246 entry persons who were tested repeatedly during the research period,9 of which were seroconversion cases so that the cumulative conversion rate was 0.1 4% (9 /6 228).The average conversion time was (6.3 ±3.0)months.There was no statistical significance between the cu-mulative seroconversion rates of the persons with different genders and nationalities (P of Fisher’s exact test >0.05).Conclusion:Serological detection of syphilis for entry personnel in Beijing port and syphi-lis-related health education should be reinforced to reduce the risk of disease importing and spreading, protect susceptible subjects and promote health.

Objective To investigate the molecular regulatory mechanism of glucagon like peptide 1 (GLP-1) on proliferation, differentiation and apoptosis of human umbilical cord blood endothelial progenitor cells (EPCs). Methods EPCs were isolated from the umbilical cord blood of healthy pregnant women and cultured in 6-hole cell plate at 2×105 density in vitro, transfected with empty vector plasmid (control group), pcDNA3-GLP-1 plasmid (GLP-1 group), pcDNA3-GLP-1plasmid+AMD3100 (GLP-1+AMD3100 group) and simple AMD3100 (AMD3100 group). The pcDNA3-GLP-1 was transfected into EPCs. The 25μmol/L AMD3100 was used to block the SDF-1/CXCR4 signal pathway of EPCs for 1 h. The cell proliferation was determined by MTT method. The mRNA expressions of differentiation and apoptosis related genes PPARγ, C/EBPα and Caspase-3 were investigated by RT-PCR, and Caspase-3 activity was determined by Caspase-3 activity assay kit. Results Compared to control group, AMD3100 inhibitor showed no effects on cell proliferation, differentiation and apoptosis, while over-expression of GLP-1 in EPCs obviously promoted cell proliferation, and differentiation related genes PPARγand C/EBPαmRNA expression, but down-regulated mRNA expression and the activity of Caspase-3 significantly (P＜0.05), indicating that GLP-1 increased proliferation and differentiation of EPCs while decreased cell apoptosis. When the SDF-1/CXCR4 signaling pathway was blocked by AMD3100, over-expression of GLP-1 induced promotion of cell proliferation, and the differentiation was decreased significantly and the apoptosis was significantly increased (P＜0.05). Conclusion These data confirm that GLP-1 might promote EPCs proliferation and differentiation, and inhibit cell apoptosis through the regulation of the SDF-1/CXCR4 signaling pathway.

Pressure ulcer care were always the focus and difficulty of nursing work.Timely and effective care were helpful to promote the repair of pressure ulcer.This article described and compared the different stages of system pressure sores assessment.This article mainly summarized the effective nursing methods at every stage of pressure ulcer at present,which were helpful to carry out evidence-based care and improve the quality of nursing.

OBJECTIVE:To compare the efficacy and safety of Jinkui shenqi pill versus Shengjing capsule in the treatment of oligoasthenozoosperimia under behavioral intervention. METHODS:98 patients with oligoasthenozoosperimia were randomly divid-ed into Shengjing capsule group(49 cases)and Jinkui shenqi pill(49 cases). All patients received intervention treatment(cognitive intervention,psychological intervention,diet intervention and exercise intervention,etc.). Based on it,Shengjing capsule group re-ceived 1.6 g Shengjing capsules,orally,3 times a day;Jinkui shenqi pill group received 6 g Jinkui shenqi pill,orally,twice a day. They were treated for 3 months. Clinical efficacy,semen quality (semen volume,sperm concentration,sperm motility rate, sperm motility)and sex hormone levels [testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(HLH),pro-gesterone(P),prolactin(PRL)] before and after treatment,and the incidence of adverse reactions in 2 groups were observed. RE-SULTS:The total effective rate in Shengjing capsule group was significantly higher than Jinkui shenqi pill group,with statistical significance(P<0.05). Before treatment,there were no significant differences in semen quality and sex hormone levels(P>0.05). After treatment,semen quality in 2 groups was significantly higher than before,semen volume and sperm motility in Shengjing cap-sule group were higher than Jinkui shenqi pill group,with statistical significances(P<0.05). T levels in 2 groups were significant-ly higher than before,while Shengjing capsule group was lower than Jinkui shenqi pill group,with statistical significances (P<0.05);and there were no significant differences in FSH,HLH,P and PRL before and after treatment in 2 groups(P>0.05). And there was no significant difference in the incidence of adverse reactions(P>0.05). CONCLUSIONS:Under behavioral interven-tion,Shengjing capsule has better efficacy than Jinkui shenqi pill in the treatment of oligoasthenozoosperimia,it can significantly improve semen quality,while Jinkui shenqi pill is better in terms of improving sex hormone levels;and both show good safety.