Objective To investigate and analyse the status and influential factors of nurses' palliative care knowledge in Shanghai city.Methods Using cluster sampling method,a questionnaire survey using Palliative Care Quiz for Nursing (PCQN) was conducted on 973 nurses in 9 hospitals of Shanghai city.Results 939 nurses completed the survey whose scores were 8.88±2.51.The status quo of palliative care knowledge of Shanghai nurses were not very desirable.The scores of palliative care philosophy and principles and psychological,social and spiritual support were 0.376±0.009 and 0.310±0.008.The scores of knowledge of the pain and symptomcontrol was 0.496±0.005,which was better than the other two aspects.There were differences between dimension 1 and dimension 2,3 (t=-1 1.78,5.465,P＜0.01),and also between dimension 2 and 3 (t=19.673,P＜0.01).Conclusions Lacking of education and training,insufficient recognition of related institutions and the limit of traditional ideas caused the defect in the knowledge structure of palliative nursing.

Immunoglobulin preparations;SARS-CoV-2;Inactivated vaccine;Neutralization test;Neutralizing antibody Objective To evaluate the neutralizing antibody level of the population to SARS-CoV-2 and its variants before and after vaccination.Methods A total of 37 samples of immunoglobulin preparations were collected. According to the time of plasma collection, taking the time of SARS-CoV-2 vaccine intensive inoculation(September 2021) and the time of epidemic prevention policy change(December 2022) as nodes, the immunoglobulin preparations produced in different time periods were divided into three groups: 19 samples of plasma collected from February 2019 to October 2021 in group 1, 8 samples of plasma collected from March 2022 to December 7, 2022 in group 2, and 10 samples of plasma collected from January 2023 to February 2023 in group 3. The neutralizing antibody levels of the wild strain, Delta strain, Omicron BA.5,BQ.1 and XBB variant strains were measured by micro neutralizing antibody assay, and the geometric mean titers(GMTs) of the antibodies were calculated.Results The SARS-CoV-2 wild strain neutralizing antibody was detected in only 5 of the 19immunoglobulin preparations prepared by plasma collected before 2021, with the titer of not more than 1 ∶ 20, and no neutralizing antibody against the other four variants was detected. From March to December 2022, the neutralizing antibody titers of eight immunoglobulin preparations against the five SARS-CoV-2 strains increased significantly, of which the wild strain(1∶2 560) and Delta strain(1∶1 810) had the highest titers, followed by the Omicron strains(GMT_(BA.5)=1∶830;GMT_(XBB)=1∶104; GMT_(BQ.1)=1∶22). Among the 10 immunoglobulin preparations prepared by plasma collected in 2023, the neutralizing antibody titers of the five strains showed little change. The GMTs of the five strains of antibodies were∶wild strain(1∶2 079), Delta strain(1∶1 114), Omicron BA.5(1∶970), Omicron BQ.1(1∶69), and Omicron XBB(1∶121). There was no significant difference in the GMTs of the five strains between the two groups of immunoglobulin preparations(t=1. 249, 1. 495, 0. 370 2, 1. 160 and 0. 502 8, respectively, each P > 0. 05).Conclusion The neutralizing antibody level of SARS-CoV-2 has significantly increased in immunoglobulin products after the implementation of enhanced inoculation of SARS-CoV-2 vaccine in China, while compared with Omicron and its branch strains, inactivated SARS-CoV-2 vaccine has better protection effect on SARS-CoV-2 wild strain and Delta strain

Objective To investigate the effect of early rehabilitation treatment in the patients with small cerebral hemorrhage and its possible mechanisms .Methods 133 patients with cerebral hemorrhage were randomly divided into the routine treatment , non-early rehabilitation and early rehabilitation groups and given the routine drug treatment .The early rehabilitation group and the non-early rehabilitation group were additionally given the rehabilitative training after 2 d and 14 d respectively .The scores of Fugl-Meyer reassessment and the Barthel index were assessed before training and after 4-week training ,and the serum levels of circulat-ing endotheial progenitor cells(EPCs) and vascular endotheial cell growth factors(VEGF) were detected .Results Compared with the routine treatment group and the non-early rehabilitation group ,the scores of Fugl-Meyer reassessment and the Barthel index af-ter treatment in the early rehabilitation group were significantly increased (P< 0 .01) .The serum levels of circulating EPCs and VEGF in the early rehabilitation group were also significantly increased compared with the routine treatment group control (P<0 .01) .Conclusion Early rehabilitation treatment can obviously improve the limb movement function and the daily living ability in the patients with small cerebral hemorrhage ,its mechanisms may be involved with the increase of circulating EPCs and VEGF .

Objective: To examine and analyze the expression of two isoforms of livin (livin α/livin β), a novel inhibitor of apoptosis protein (IAP) family member, in tumor cell lines and malignant tumor tissues and analyze the relationship between livin expression and clinical pathological features of different tumors. Methods: Livin mBNA expression was detected by reverse transcription polymerase chain reaction (BT-PCR) in 12 malignant tumor cell lines, 50 different malignant tumor tissues, and 20 cases of normal tissues. Results: Livin mBNA was highly expressed in seven cell lines (Hela, SPEA-1, SBE-2, PC14, MKN45, LOVO, and HHCC) and poor expressed in A549 cells, It had negative expression in other four kinds of cell lines ( HL-7702, Hep-2, 7721 and K562). Livin mRNA was positively expressed in 32 of 50 malignant tumor cases (64.0%). The expression of livin mRNA was at low level in paracancerous tumors tissues (2%). Livin expression had no significant association with differentiation degree, lymph node metastasis and TNM staging of tumor patients. Conclusion: Livin expression in tumor cell lines and malignant tumor tissues provides stronger evidence for further investigating the relationship between livin and tumor initiation and development. It may serve as a new target for malignant tumor diagnosis and therapy.

Cells, Cultured ; Humans ; Neovascularization, Physiologic ; immunology ; Pericytes ; metabolism ; physiology

Objective To study on the immunogenicity of SAK and its mutant using PAb.Methods 4 New Zealand white rabbits subcutaneously injected with corresponding antigens were grouped into group SAK involving 2 rabbits and group SAK2 involving 2 rabbits.The antiserums were collected 1 week after third injection.The rabbit IgG fraction was precipitated with saturated(NH4)2SO4 and purified by DEAE-Sepharose column chromatography,and then the antibody titer was determined by ELISA.The immunoreactivity of antigen to polyclonal antibody against SAK and SAK2 was tested using ELISA.Results The immunoreactivity of SAK2 to polyclonal antibody against SAK was sharply reduced to a very low level determined by ELISA,which indicated that some epitopes of SAK had been deleted.Conclusions The immunoreactivity of SAK to polyclonal antibody against SAK2 did not get a dramatically change compared with SAK2,which indicated that the reconstruction of epitope did not create new epitope.

[Objective]To compare the clinical results between allograft and autograft for treatment of scoliosis in the adolescent.[Method]A retrospective study was made in 63 cases of adolescent patients with scoliosis who were operated on with posterior instrumentation from 1996~2006.These patients were divided into two groups.In group A,32 patients aged 10 to 15 years with an average of 12.2 years,had a mean Cobbs angle of 62?(ranged,38? to 113?),were received allograft bone grafting.The patients were followed up for at least 18 months with an average of 26 months.Clinical and radiographic evaluations were performed.[Results]Operating time and blood loss in group A were less than that in group B(P

Objective To investigate molecular changes in growing rats masticatory muscle after functional mandibular protrusion.Methods SD rats were randomly divided into eight groups as control 3d,7d,14d,21d and experimental 3d,7d,14d,21d.The RTPCR and qPCR methods were used to find expression and any differences of mRNA of MMPs in growing rats masticatory muscle after functional mandibular protrusion. (Results)(① Expression) of MMP-2,MMP-9 mRNA expressed in all growing rats masticatory muscle.② Expression differences of MMP-2 and MMP-9 mRNA in 3d,21d were found between control and experimental groups after functional mandibular protrusion.Conclusion ①MMP-2 and MMP-9 mRNA participate in masticatory muscle normal development.(② MMPs) mRNA involve changes in growing rats masticatory muscle after functional mandibular protrusion.

AIM: To construct the eukaryotic expression vector CD80-IgG by fusing the cDNA encoding extracellular portion of murine CD80 to the 5'-terminus of cDNA encoding Fc fragment of murine immunoglobulin G1 and to express the fusion protein in Chinese hamster ovary (CHO) cells. METHODS: The two cDNAs was amplified by PCR respectively from plasmid pcDNA/B7 containing the full-length cDNA of murine CD80 from murine spleen cells, and cloned to the eukaryotic expression vector pcDNA3.0 by directional cloning. The resultant recombinant plasmid pcDNA/CD80-IgG was transfected into CHO cells with liposome transfection reagent. The stably expressing cells were obtained by G418 screening. Western blot, Dot ELISA, and flow cytometry were used to detect the expression of the fusion protein and its immunological activity. RESULTS: DNA sequencing verified the correction of the construction of recombinant plasmid pcDNA/CD80-IgG. The expressed fusion protein was detected in the supernatant of transfected CHO cells and the molecular weight of the protein was similar to what we expected. Its immunological activity was also established. CONCLUSION: The recombinant plasmid pcDNA/CD80-IgG was successfully constructed and it expressed the fusion protein CD80-IgG.