Object To provide an economical and practical technique for planting of Radix Gentianae in large scale Methods Indoor artificial pregermination, insemination in carboxymethyl cellulose suspension, irrigation with micro sprayer and no shelter growing technique of seedling Results The seedling cost is 60% lower than that of conventional technique In each square meter 3 000 seedlings were grown Conclusion This technique can be widely applied.

Object To investigate the reason of the low germination and sprout rates by studying on inhibitory activities of the crude extracts of the seed of the dried root of Saposhnikovia divaricata (Turcz.) Schischk (DRSD) on Brassica, wheat and the seed of DRSD. Methods The ether extract and residue of the crude extract were prepared and treated by paper chromatography. The inhibitory activities of different fractions with different Rf value were determined. Effect of steeping the seed in warm water at 41 ℃ and 45 ℃ for different periods of time was also studied, respectively. Results There are intrinsic inhibitor in the seed of DRSD with strong activity. The portion of its ether extract with Rf 0.6 showed the strongest inhibitory activity for the germination of Brassica seed. The crude extract of the seed of DRSD also has the stronger inhibitory activity to the germination and growth of tender root of S. divaricata and has the same effect on aerial part of wheat sprouts. Steeping with warm water and solvent can remove most of the intrinsic inhibitor. Conclusion Intrinsic inhibitor in the seed of DRSD is the main factor that results in the low germination and sprouting slowly after seeding.

10 adult female rats were used to study blast injuries of the air-way and lung. The conclusions are as follows. 1. There was significant difference in arterial blood gas analysis between the normal control and injury group. 2. The une-venness in the lung damage, bleeding, tear of the alveolar septa induced by the air shock wave was predominant, by scanning electron microscopy (SEM) as well as by the light microscopy (LM) . The bleeding may be due to the capillary rupture of alveolar septa. The' rope-like' structures seen might represent the fibre component in the septa which had more resistance to the local force and remained after the other components were wiped away by explosion. 3. Striping and fissure of the epithelium of trachea and bronchi of the injured group were detected. 4. The pathological changes observed with SEM were compared to those with LM.

The morphological changes of the rat lung with blast injury are quantitified with a stereological method. After blast the pulmonary capillary volume and internal surface area decreased significantly, arithrnatic and harmonic mean thickness of the air-blood barrier, the volumes of P'MN and platelet in capillury increased remarkably. The morphometric lung diffusion capacity of oxygen (DLO2) is reduced markedly mainly due to the decrease of diffusion capacity of air-blood barrier and erythrocyte. The change of DLO2 is closely correlated with the arterial blood oxygen pressure, indicated that the damage of pulmonary capillary is one of the main causes of alveolar bleeding and the early respiratory insufficiency. A mathematic relation can be established between the intensity of the blast wave and the corresponding morphological changes of the injured lungs.

Semaphorins is the one of guidance molecule families.It is proved to have wide physiological function in the development of neutron and play important role in the pathogenesis of retinal degenerative diseases,gangliocyte apoptosis-related diseases and neovascularization.Previous studies showed that Semaphorins distribute in the cornea,lens and retina.Semaphorins play important role in the development of retina,visual system,cornea nerve and reduce the death of retinal ganglion cells(RGCs)caused by glaucoma,retinal detachment and hypoxic ischemic optic neuropathy.Semaphorin family-related gene mutation may be associated with retinal degenerative diseases.Some of the members could also inhibit angiogenesis.Since semaphorins and its receptors participate in the development of the vasculature and nervous systems,they are implied to be the potential new target of ophthalmopathic treatment.The current ptogress in structure and functions of the semaphorin family along with their relationship with ophthalmopathy were summarized in this study.

Cardiomyopathies ; chemically induced ; Chlorine Compounds ; poisoning ; Female ; Humans ; Middle Aged ; Oxides ; poisoning

Objective To study the role of ARHI protein and COX-2 protein and to explore their correlation in pathogenesis of adenocarcinoma of the uterine cervix.Methods The expression of ARHI and COX-2 protein were detected both in 40 cases of adenocarcinoma of the uterine cervix and in 30 cases of normal cervix by immunohistochemistry.Results The positive rate of the expression of ARHI protein in adenocarcinoma of the uterine cervix was 50.0％ (20/40),which lower than that in normol cervix controls 90.0％ (27/30),the difference was significant ( x2 =12.43,P ＜ 0.05 ) ; The positive rate of the expression of COX-2 protein in adenocarcinoma of the uterine cervix was 82.5 ％ (33/40),which higher than that in normol cervix controls 0 (0/30),the difference was significant (x2 =46.16,P ＜ 0.05 ) ;The positive expression rate of ARHI was negatively correlated with the clinical stage of cervical adenocarcinoma ( x2 =3.96,P ＜ 0.05 ) disease and pathological grade (x2 =7.04,P ＜ 0.05 ).COX-2 protein was positively correlated with pathological grade (x2 =7.04,P ＜0.05 ),but had no correlation with the clinical stage of cervical adenocarcinomacervical adenocarcinoma stage of diseas(P ＞ 0.05) ;The expression of ARHI protein and COX-2 protein wasn't correlated with lymph nodeatic meatastasis(P ＞ 0.05);The expression of ARHI protein had correlation with the expression of COX-2 protein (r =0.31,P ＜ 0.05 ).Conclusion Inactivation of ARHI gene and the abnormal expression of COX-2 protein are involved in the origin of cervical adenocarcinoma,their synergistic reaction may play an important role in tumor origin of cervical adenocarcinoma.

OBJECTIVE:To provide reference for the standardization of online pharmacies pharmacist service. METHODS:Lit-erature research method,online entity investigation method and the method of mysterious customer were adopted to investigate 10 well-known online pharmacies,then described the analysis of current online pharmacies pharmacist service in China. RESULTS:There were 5 online pharmacies showing pharmacist service,but only 1 pharmacy could provide pharmacist registration certificate;on average each online pharmacy was allocated with 1.9 licensed pharmacists;only 2 online pharmacies provided 24 hours of phar-macist service;8 online pharmacies provided more than 2 consulting method;messagebox would pop actively when clicking the“Buy”button in 6 online pharmacies;the correct rate of pharmacist’s guidance was less than 60%,only 20% pharmacists could provide right explanation. CONCLUSIONS:The reasons of the lack of online pharmacies pharmacist service include online pharma-cies imperfect laws and regulations and supervision,lack of licensed pharmacists quantity and quality is not high enough,online pharmacies,pharmacists consulting system is not mature. Online pharmacies can improve relevant laws and regulations,strengthen the government supervision,reasonable and effective use of licensed pharmacists resources,perfect the pharmacist online consult-ing service water system and improve the licensed pharmacists to improve pharmacists service function status.

BACKGROUND: In vivo animal experiments have found that magnesium al oy stents can be completely degraded in a slow, orderly and non-toxic manner fol owing the vascular remodeling. OBJECTIVE: To investigate the applied effect and biocompatibility of a new type of magnesium al oy stent in blood vessels. METHODS: Forty-two crossbreed dogs were implanted with absorbable magnesium al oy stents via the coronary artery or left femoral artery. At days 1, 3, 5 and weeks 1, 2, 3, 4 after implantation, coronary or femoral artery angiography, inflammatory factor detection and morphological analysis targeting intimal hyperplasia were performed. RESULTS AND CONCLUSION: At 1 day after implantation, the stents had the complete shape and were ful y extended; at 3 days after implantation, the stents were degraded partial y; at 1 week after implantation, the stents were degraded completely. Within 1-3 weeks after implantation, the levels of tumor necrosis factor α, interleukin-6 and interleukin-8 continued to decrease. At 2 and 3 weeks after implantation, the target vessels presented with mild intimal hyperplasia. As time went on, the hyperplasia area increased gradual y, and the percentage of intimal hyperplasia increased gradual y. Blood magnesium concentration remained unchanged before and after stent implantation. The results show that the new absorbable magnesium al oy stent can be completely degraded within 1 week after stent implantation and has good biocompatibility.

Objective To investigate the turnover and factors influencing amniotic membrane transplantation into corneal stroma.Methods Amniotic membrane stained with dichlorotriazinyl aminofluoresclin (DTAF)was implanted into the corneal stroma of 1 5 New Zealand white rabbits.After transplantation,we observed the clinical changes.The tissue samples from grafted area were observed with HE staining and laser confocal microscope to trace amniotic membrane at 4,8,13,1 7 and 22 week after surgery.And the tissue samples at week 22 after surgery from grafted area were also observed by transmission electron microscopy.Results After 4 weeks of operation, amniotic membrane tissue bent with visible multi-layer homogeneous red dye.The structure remained intact;basement membrane and stroma were no dissolved.After 8 weeks, amniotic tissue was a strip structure of homogeneous red dye,and the substance layer was complete.After 13 weeks,amniotic membrane morphology appeared fuzzy,but still took the shape of strips;a small number of corneal cells accumulated around the amniotic membrane.At 1 7 weeks after operation,fragmented amnion was evident.At 22 weeks after operation,small pieces of amnion tissue remained in the corneal stroma.Laser scanning confocal microscopy revealed that amniotic membrane could retain for over 22 weeks in the corneal stroma.After 22 weeks,amnion stroma collagen fibers that were integrated into the corneal stroma were irregular.Corneal stromal ultrastructure surrounding the amniotic membrane did not obviously change.Conclusion Retention time of amniotic membrane transplanted into corneal stroma can be as long as over 22 weeks and amniotic membrane can be merged into the corneal tissue as a substitute, which wins time for corneal transplantation operation.