2.Primary IgA nephropathy in 46 children: association of clinical and pathological findings with prognosis.
Ya-ping WANG ; Ai-min LIU ; Yu-wen DAI
Chinese Journal of Pediatrics 2005;43(11):866-867
Adolescent
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Child
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Female
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Glomerulonephritis, IGA
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diagnosis
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pathology
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Humans
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Male
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Prognosis
4.Expression and clinical significance of cathepsin K in intervertebral disk degeneration in humans
Ya LIU ; Zongliang WANG ; Ming CAI ; Min WANG ; Shang WANG
Chinese Journal of Tissue Engineering Research 2010;14(7):1319-1321
BACKGROUND: Several studies have confirmed that activation of intervertebral disc enzymes is closely related to matrix degradation. Matrix metalloproteinase and tissue inhibitor of metalloproteinase have been shown to exert important roles in the process of extracellular matrix degeneration in intervertebrel disk. Besides these two enzyme systems, whether other proteeses that exhibit degrading effects on extracellular matrix are involved in the intervertebral disk degeneration remains poorly understood.OBJECTIVE: To detect the cathepsin K expression in normal and degenerated human intervertebrel disc cells and investigate the correlation between cathepsin K and intervertebral disc degeneration.METHODS: Cathepsin K expression was detected in intervertebral disc tissue from 30 patients with lumbar intervertebral disc protrusion using immunohistochemistry SP method and ELISA. At the same time, the intervertebral disc tissue from 15 healthy adult cadavers and/or spine fracture patients was taken as control, Cathepsin K protein expression in normal and degenerated human intervertebral disc tissues were compared.RESULTS AND CONCLUSION: Cathepsin K expression was observed in normal and degenerated intervertebral disc tissues.The expression level was significantly higher in degenerated tissue than normal tissue (P < 0.05). These findings demonstrate that Cathepsin K possibly participates in the intervertebral disc degeneration.
5.Effects of Shenqi Compound Recipe on expression of COX-2 mRNA in aorta in GK rats
Min CHEN ; Hong GAO ; Chunguang XIE ; Ya LIU ; Can ZHUANG
China Journal of Traditional Chinese Medicine and Pharmacy 2006;0(09):-
Objective:To explore the effects of Shenqi Compound Recipe on the expression of Cycloxygenase-2 (COX-2)mRNA in aorta in GK rats.There were five groups:GK group,model group,atorvastatin group,Shenqi Compound Recipe group and normal control group.During the experiment periods,each group was administrated correspondent substance respectively for 35 days.Serum concentrations of C reactive protein(CRP)were determined by ELISA.The mRNA expressions of COX-2 in aorta were detemined by reverse transcriptase PCR(RT-PCR).Results:Compared with model group,concentrations of CRP in serum and the mRNA expression of COX-2 all decreased in atorvastatin group and Shenqi Compound Recipe group(P
6.Analysis laboratory results of three cases of COVID-19 complicated with falciparum malaria
ZHAO Rong ; SHI Ya-ling ; LIU Yan-xia ; JIANG Hong-ya ; FAN Yin-min ; ZHANG Zhan-feng
China Tropical Medicine 2023;23(1):106-
Abstract: Objective To analyze the laboratory indexes of patients infected with malaria patients and COVID-19, so as to provide reliable evidence for the diagnosis of mixed infection of both. Methods The routine clinical laboratory items such as routine blood, biochemistry and lymphocyte subsets were tested in three cases of COVID-19 complicated with falciparum malaria who admitted to Guangzhou Eighth People's Hospital Affiliated to Guangzhou Medical University from July to December 2020 were tested. Laboratory data were stage-wise analyzed in conjunction with changes in the course of disease. Results Three patients confirmed COVID-19 infection recruited all had malaria infection history. Fever, headache, and other symptoms emerged on the 4rd to 11th day after admission. Malaria parasite was detected by malaria parasite antigen testing and blood smear testing, and all three patients had re-ignition of malaria after being confirmed COVID-19 infection. In the early stage of malaria relapse, lymphocytes decreased, CRP and SAA increased, and gradually returned to normal level after antimalarial treatment. Interestingly, we only found one patient at the initial stage of malaria detection showed PLT decreased, no other unnormal changes in other routine blood results (WBC, ESO) and liver function results (ALT, AST, GGT, TBIL, DBIL, CG) were found from the beginning to end course of the disease. Conclusion COVID-19 infection may promote the resurgence of malaria, so the relapse of malaria should be monitored especially for the patient with malaria infection history who begin to develop fever and other symptoms a few days after the diagnosis of COVID-19. The inflammatory indicators would be worth able as an auxiliary judgment basis for the effective treatment of the two combined infection.
7.Neuroprotective effect of longistyline A against corticosterone-induced neurotoxicity in PC12 cells.
Bao-Ping JIANG ; Rui-Wu YANG ; Xin-Min LIU ; Ya-Min LIU ; Qi CHANG ; Jian-Yong SI ; Rui-Le PAN
Acta Pharmaceutica Sinica 2012;47(5):600-603
This study is to investigate the protective effect of longistyline A against corticosterone-induced neurotoxicity in PC12 cells. While PC12 cells were exposed to 100 micromol x L(-1) corticosterone for 48 h, cell survival rate was reduced and lactate dehydrogenase (LDH) release increased. In parallel, corticosterone caused significant elevations of DNA fragmentation, [Ca2+]i and caspase-3 activity. However, when the PC12 cells were incubated with longistyline A (4.0, 8.0 and 16.0 micromol x L(-1)) in the presence of 100 micromol x L(-1) corticosterone for 48 h, the effects were evidently alleviated, but dose-dependent manner was not obvious. In summary, longistyline A could generate a neuroprotective effect against corticosterone-induced neurotoxicity in PC12 cells possibly by decreasing [Ca2+]i and caspase-3 activity.
Animals
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Cajanus
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chemistry
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Calcium
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metabolism
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Caspase 3
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metabolism
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Cell Survival
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drug effects
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Corticosterone
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toxicity
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DNA Fragmentation
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drug effects
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L-Lactate Dehydrogenase
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metabolism
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Molecular Structure
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Neuroprotective Agents
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isolation & purification
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pharmacology
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PC12 Cells
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Phenols
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isolation & purification
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pharmacology
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Plant Leaves
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chemistry
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Plants, Medicinal
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chemistry
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Rats
8.A new cyclic diarylheptanoid from the bark of Myrica rubra.
Sheng-nan SHEN ; Fang-bo XIA ; He LI ; Ya-min LIU ; Rui-le PAN
Acta Pharmaceutica Sinica 2015;50(6):746-748
To study the chemical constituents from the bark of Myrica rubra, fourteen compounds were isolated from the methanolic extract using various chromatographic techniques, including silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified on the basis of chemical properties and spectroscopic data, as 3, 5-dimethoxy-4-hydroxymyricanol (1), myricanol (2), myricanone (3), myricanol 11-sulfate (4), myricitrin (5), quercetin (6), quercetin-3-rhamnoside (7), tamarixol (8), uvaol (9), ursolic acid (10), taraxerol (11), myricadiol (12), β-sitosterol (13) and β-daucosterol (14). Among them, compound 1 is a new compound, named as 3, 5-dimethoxy-4-hydroxymyricanol, compounds 8, 9 were isolated from the genus Myrica for the first time.
Diarylheptanoids
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chemistry
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isolation & purification
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Myrica
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chemistry
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Phytochemicals
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chemistry
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isolation & purification
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Plant Bark
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chemistry
9.Establishment and identification of HEK293 cell lines with stable and high expression of NTCP
Ya CHEN ; Jing LI ; Wenzheng ZHANG ; Min LUO ; Xiaozhong FU ; Ting LIU
Chinese Pharmacological Bulletin 2016;32(12):1767-1771,1772
Abstrate:Aim To construct HEK293 cell line with stable and high expression of sodium taurocholate cotransporting polypeptide (NTCP ) efficiently and rapidly.Method Vector expressing EGFP-NTCP fusion protein was constructed and verified by DNA sequencing.The pEGFP-NTCP expression vector was transfected into HEK293 cells by FuGENE 6 transfection reagent. The transfected cells with high expression of green fluorescent protein were selected using fluorescence microscope for screening of G418 for 14 days to obtain cell lines stably and highly expressing NTCP.NTCP expression was detected by RT-PCR,qRT-PCR, Western-blot and the uptake experiment of taurocholic acid.Re-sult RT-PCR,qRT-PCR,Western-blot and the uptake experi-ment revealed that compared to the control cells,the expression of NTCP was significantly positive (P<0.01)in stable trans-fected cells showing green fluorescence (P<0.05 ).Conclusion The HEK293 cell line with stable and high expression of NTCP has been established efficiently and rapidly,which provides a cellular model for the study of the mechanism of the uptake of bile acid derivatives.
10.Comparison of hPepT1 transfected MDCK cells to hPepT1 transfected HeLa cells
Min LUO ; Xiaozhong FU ; Tao XIAO ; Wenzheng ZHANG ; Jing LI ; Ya CHEN ; Ting LIU
Chinese Pharmacological Bulletin 2017;33(2):280-284
Aim To screen a more suitable transfection recep-tor,and improve the efficiency of constructing cell lines highly expressing human peptide transporters 1 (hPepT1 ).Methods The recombinant plasmid pcDNA3.1 (+)-hPepT1 was transfect-ed into MDCK cells and HeLa cells by LipofectamineTM 2000 transfection reagent,respectively.The monoclonal cells were se-lected and cultured.Expression of hPepT1 mRNA and protein were determined by qRT-PCR and Western blot,respectively. The uptake capacity of Glysar in transfected cells was examined. Results Compared with wild type cells,the expression of hPepT1 and the uptake of Glysar in transfected MDCK cells and HeLa cells significantly increased (P <0.05).Although the up-take of Glysar in HeLa cells was higher than that of MDCK cells,on the contrary,the expression of hPepT1 and the uptake of Glysar in MDCK-hPepT1 cells was higher than that of HeLa-hPepT1 cells.Conclusion MDCK cells may serve as a more suitable transfected receptor for the construction of a cellular model with high expression of hPepT1 ,which would make the construction of a cell model highly expressing hPepT1 more effi-cient.