To observe the clinical efficacy of moxibustion plus cupping in treating trigeminal neuralgia due to wind-cold.Method Seventy patients with trigeminal neuralgia due to wind-cold were randomized into a treatment group and a control group, 35 cases in each group. The treatment group was intervened by moxibustion plus cupping, while the control group was by ordinary acupuncture. The Visual Analogue Scale (VAS) and other pain indexes were observed before treatment and after 2 treatment courses, and the clinical efficacies were compared.Result The total effective rate was 85.7% in the treatment group versus 57.1% in the control group, and the difference was statistically significant (P<0.05). The VAS scores were significantly changed after treatment in both groups (P<0.01). After treatment, the VAS score of the treatment group was significantly different from that of the control group (P<0.01). After intervention, the scores of pain range, pain intensity, pain duration, attack frequency, quality of life, affection, and global pain score in the treatment group were significantly different from that in the control group (P<0.05).Conclusion Moxibustion plus cupping is an effective approach in treating trigeminal neuralgia due to wind-cold.

2-(3-benzoylphenyl)propionitrile which is an important intermediate of ketoprofen was synthesized through phase-transfer catalytic by four steps, involving Friedel-crafts reaction, bromination and cyanation. Pure compound was obtained, and the content of crystal was about 98%.AIM　The content of 2-(3-benzoylphenyl)propionitrile was improved. METHODS　Phase-transfer catalytic methylation and suitable method of aftertreatment were used. RESULTS　The yield and content was both improved. CONCLUSION　Phase-transfer catalytic methylation was the best method, and suitable method of aftertreatment was essential.

Objective:To establish an HPLC method for the determination of gallic acid,quercetin and kaempferol in Gongyanping capsules. Methods:HPLC was applied with the chromatographic conditions as follows: The chromatographic column was Agilent-SB C18 (250 mm × 4. 6 mm,5 μm) at 30℃; the mobile phrase was methanol-0. 3% phosphoric acid solution with gradient elution; the flow rate was 1. 0 ml·min-1. Results: The linearity relationship of gallic acid, quercetin and kaempferol was within the range of 98.200-491.00 μg·ml-1(r=0.999 9), 7.520-37.600 μg·ml-1(r=0.999 9) and 4.940-24.700 μg·ml-1(r=0.999 9), re-spectively;the average recovery was 96. 74%(RSD=1. 33%), 98. 18%(RSD=1. 70%) and 97. 04%(RSD=1. 28%),respective-ly. Conclusion:The method is simple, accurate and repeatable.

The receptor for advanced glycation end product (RAGE) is a member of the immunoglobulin superfamily of cell surface molecules.As a pattern recognition receptor,it can bind with a variety of ligands.The adult lung is unique in which it expresses high amounts of RAGE under normal conditions while other tissues express low amounts normally and up-regulate RAGE during pathologic processes.The lung exhibits high basal level expression of RAGE localised mainly in alveolar type I cells,suggesting a potentially important role for the receptor in epithelial cell proliferation and differentiation and maintaining lung homeostasis.Indeed,disruption of RAGE levels has been implicated in the pathogenesis of a variety of pulmonary disorders such as acute and chronic lung injury.Furthermore,its soluble form sRAGE,as a decoy receptor,has been shown as an important marker of alveolar epithelial type I cell injury.Studies have suggested that RAGE plays an important role in the biology of the lung and may become a therapeutic target for lung injury.

Objective To study the effect of alcohol on apoptosis and apoptosis-associated genes-Bcl-2 and Bax of cardiac muscle cell.Methods We established an animal experimental model by giving different concentration of alcohol to the healthy mice.Myocardial apoptosis and the expression of apoptosis-associated gene Bcl-2 and Bax were quantitatively analysed by the nuclear fast red-crystal violet staining and immunohistochemical method.Results Some nuclears of the experimental groups were stained purple and agglutinated.Counting under the high power microscope,it was found that the amount of abnormal nuclears significantly increased in alcohol-treated groups compared with the control group (P

RKIP (Raf kinase inhibitor protein) is a member of PEBP (phosphatidylethanolamine-binding protein) family, which plays a pivotal modulatory role in MAPK (mitogen-activated protein kinase), CPCR (G-protein-coupled receptor) and NF-KB (nuclear factor-kappa B) signaling transduction pathways. In recent years, many studies reported that RKIP expression is weakened or lost in a series of malignant tumors such as prostate cancer, breast cancer and melanoma. RKIP may also play an important role in inhibition of tumor metastasis via inhibition of cell invasion and tumor angiogenesis. Recently, many experiments revealed that RKIP which is a metastasis-suppressor gene participates in the suppression of metastasis in a variety of digestive system malignant tumors and it will become a new target for prevention and control of digestive system cancer. In this article, the advances of research on RKIP in common digestive system malignant tumors were reviewed. Copyright © 2013 by TUMOR.

AIM To explore whether or not the function of insulin like growth factor (IGF I) in promoting osteopontin gene expression and protein synthesis of rat vascular smooth muscle cell (VSMC). METHODS Cultured cells were incubated with different concentration of IGF I for different hours, then RT PCR and Western blotting analysis were used to observed the effects of IGF I on osteopontin expression of rat vascular smooth muscle cells RESULTS IGF I (20 ?g?L -1 ) could obviously induce the gene expression and protein synthesis of osteopontin, RT PCR results showed that the ratio of mRNA of osteopontin and ? actin in cells incubated with IGF I (20 ?g?L -1 ) for 3, 24 and 48 h increased by 39 70%,40 44% and 54 05% respectively, as compared with that of the control ( P

Euthyroid Sick Syndromes ; blood ; etiology ; Female ; Gestational Age ; Humans ; Hypothyroidism ; blood ; etiology ; Infant, Newborn ; Infant, Premature ; Male ; Risk Factors ; Sex Factors ; Thyronines ; blood ; Triiodothyronine ; blood

Adiponectin is a kind of adipocyte-specific protein which is only inversely related with obesity by now. It is involved in enhancing insulin sensitivity,anti-atherogenic and antiinflammatory activities.Recent studies have reported that adiponectin is closely correlated with the genesis and development of a variety of obesity related malignant tumors,especially postmenopausal breast cancer.Obesity is an independent risk factor for the development of breast cancer.Recently researches about the correlation between adiponectin and breast cancer relations and the action mechanisms,signaling pathways have made a progress.

By analyzing the assessment of the recruit military doctors graduating from civil medical schools for their training pattern,some advantages and disadvantages about the current training pattern are discussed.According to the result,some suggestions were given to the training content,curriculum setting,curriculum duration and so on.