Objective: To observe the clinical efficacy of different media in pediatric tuina treatment for children with muscular torticollis. Methods: A total of 80 children with muscular torticollis were randomized into an observation group and a control group, 40 cases in each group. Both groups received conventional pediatric tuina therapy. The observation group was treated with Bai Mai ointment as the massage medium, while the common lubrication oil was used as medium in the control group. Both groups were treated five times a week, 20 min for each time, 20 times constituted a course of treatment. The traditional Chinese medicine (TCM) syndrome scores were evaluated before and after treatment, and the curative effect was observed after 3 courses and 6 courses of treatment respectively. Results: The TCM syndrome scores improved in both groups after treatment, and the intra-group differences were statistically significant (both P<0.01). The score of the observation group was statistically different from that in the control group (P<0.05). In the observation group, the total effective rates after 3 courses and 6 courses of treatment were 95.0%and 100.0%, respectively, versus 87.5% and 95.0% in the control group. The total effective rates of the two groups were statistically different (both P<0.05). Conclusion: Bai Mai ointment has a better curative effect than the common lubricating oil as a massage medium in the treatment of children with muscular torticollis.

The pathogenesis of congenital heart disease can not be explained by a simple genetic mechanism.Currently,it is recognized by the interaction of genetic and environmental factors.The environmental factors change epigenetic conditions in body.Methylation is the earliest research aspect in epigenetics.However,methylation in congenital heart disease is not being given enough attention until recent years.Therefore,this review describes the relationship between methylation and congenital heart disease,in order to make us understand the pathogenesis of congenital heart disease in-depthly.

Objective:To investigate the effect and the mechanism of interleukin-18 binding protein(IL-18BP) on acute graft-versus-host disease(aGVHD) in allogeneic bone marrow transplantation(allo-BMT) in mice.Methods:C57BL/6 recipient mice irradiated with 60Co were randomly divided into 2 groups: group A and B and were transfused with BALB/c donor cells.After transplantation recipient mice in group A were treated with NS subcutaneously daily from day 0 to day 5 and recipient mice in group B with 1 ?g IL-18BP,separately.The severity of aGVHD was evaluated in the two groups.Concentrations of IFN-?,IL-2,IL-4 and IL-10 were measured using enzyme-linked immunosorbent assay(ELISA).Results:aGVHD response was more severe in group A than that in group B.The serum concentrations of IFN-? and IL-2 in group A were higher than that in group B,while the serum concentrations of IL-10 and IL-4 were lower than that in group B.Conclusion:①IL-18BP can decrese aGVHD response;②That IL-18BP can promote the differentiation of CD4~+T cells into CD4~+Th2 cells,that is promoted by IL-18BP might be one of the mechanisms of decreasing aGVHD.

Objective To observe the therapeutic effect of Swertia mussotii Franch is extracted by alcohol on immunological liver injury in mice,and further evaluate its immun-regulating effect in decreasing aminotransferase and protecting heptocytes. Methods BCG vaccine and lipopolysaccharide were intravenously injected to establish liver injury model.The effects of Swertia mussotii Franch on liver injury were contrasted. Results The high-,medium-and low-dose Swertia mussotii Franch groups which had been administered for 10 consecutive days,had significantly inhibited serum ALT and AST acticities of immunological liver injury;Swertia mussotii Franch could effectively prevent immunological liver injury by BCG+LPS. Conclusion Swertia mussotii Franch can protect mice from immunological liver injury and protect the function of liver.

In this paper, anatomy of the liver and voluminal percentage of the hepatic segments in whole liver in twenty-two men's liver were studied.The results revealed that the average volume of the liver is 1289 cm3; the mean volume is 22.3cm3 in segment I, and is 9.9%,11.6%, 16.6%,17.0%,14.6%,14.7%,15.6% insegment II to VIII respectively.

Objective Through the UVB rays radiation skin injury model ,the study investigated the effect of seabuckthorn flavonoids on the protection and restoration of the skin ,in order to provide experimental basis of looking for the new drugs against ultraviolet injury .Methods By UVB radiating repeatedly ,the study built UVB radiation skin injury model in mice ,to observed the morphological changes of the skin in every groups of experimental animal ,the super oxide dismutase (SOD) activity of the skin and the peroxidation damage degree of the skin lipid ,which is also the content changes of malondialdehyde (MDA) and collagen .Results The change of model group whose epidermis layer increased and the dermis thickened was more obvious than the normal group ,so the UVB radiation skin injury model was built successfully .The model group compared with the normal group ,its SOD activity was significantly lower and its MDA content was significantly higher (P< 0 .01) .However ,the SOD activities of positive control group and being fed with seabuckthorn flavonoids group were significantly higher and their MDA contents were significantly lower (P<0 .01) .The model group compared with the normal group ,its collagen content was significantly lower (P< 0 .05) ,and the collagen contents of positive control group and being fed with seabuckthorn flavonoids group were higher ,whose difference was extremely significant(P< 0 .01) .However ,the positive control group had hyperplastic scar .Conclusion Seabuckthorn flavonoids had the fol‐lowing effects :preventing the UVB repeating radiation damage to the skin ,improving the SOD activity of skin tissue and the colla‐gen content ,inhibiting the increase of MDA content and scar hyperplasia ,and having antagonism effect of UVB causing mice skin photoaging .

Objective To investigate the clinical effect ofKudiezi injection combined with rosuvastatin on patients with acute cerebral infarction(ACI).Methods A total of 90 patients with ACI patients of Department of Neurology of our hospital, from March 2015 to June 2016, were randomly divided into treatment group Ⅰ, treatment group Ⅱ and the control group, 30 in each group. The control group was given atorvastatin, the treatment group Ⅰ received rosuvastatin, and the treatment group Ⅱ was adopted Kudiezi injection combined with rosuvastatin. The curative effect was observed, and blood lipid and coagulation function were compared, and daily life ability and nerve function were recorded.Results The curative ratein treatment group Ⅱ (96.7%)was significantly better than that in treatment group Ⅰ (76.7%) and the control group (73.3%) (χ2=6.537,P<0.05). Total cholesterol,three acyl glycerin,low density lipoprotein in treatment group Ⅱ (4.03 ± 0.83 mmol/L, 1.68 ± 0.46 mmol/L, 2.37 ± 0.48 mmol/Lvs. 4.61 ± 0.89 mmol/L, 2.02 ± 0.81 mmol/L, 2.74 ± 0.68 mmol/L and 5.42 ± 0.75 mmol/L, 2.38 ± 0.67 mmol/L, 3.11 ± 0.81 mmol/L) were significantly lower than those in treatment group Ⅰ and the control group (F=21.538, 8.585, 9.092,P<0.05), and those in treatment group Ⅰ was significantly lower than those in the control group (P<0.05).The fibrinogen (2.48 ± 0.37 g/Lvs.2.81 ± 0.46 g/L, 2.95 ± 0.51 g/L), platelet counts[(125.27 ± 11.88)×109vs.(132.13 ± 13.55)×109 and (133.83 ± 13.91)×109] and prothrombin time (17.52 ± 1.94 svs.(16.14 ± 1.62 s, 15.34 ± 1.18 s) in treatment group Ⅱ were significantly lower and higher than those in treatment group Ⅰ and control group (F=8.592, 3.571, 14.139,P<0.05). The scores of Barthel index (85.63 ± 4.10 vs.81.83 ± 3.92, 79.23 ± 4.81) and NIHSS (3.57 ± 1.17 vs. 4.63 ± 1.22, 5.57 ± 1.33) intreatment group Ⅱ were significantly higher and lower than those in treatment group Ⅰand the control group (F=16.873, 19.550,P<0.05), those in treatment group Ⅰ was significantly higher and lower than those in the control group (P<0.05).ConclusionsKudiezi injection combined with rosuvastatin could improve curative effect ,reduce blood lipid, improve coagulation function, improve quality of life and promote recovery of nerve function in patients with acute cerebral infarction.

Objective To assess the value of laparoscopy combined with hysteroscopy in the diagnosis and treatment of the ovulatory infertile female.Methods From Jan 2000 to May 2001, 140 ovulatory infertile patients were investigated and treated by laparoscopy combined with hysteroscopy. Findings of both the laparoscopy and hysteroscopy were compared with those of hysteroscopy alone and laparoscopy alone. Operative time, postoperative complications and days of hospitalization were recorded. Postoperative pregnancy outcomes were followed-up.Results This study demonstrates that combination of laparoscopy and hysteroscopy was superior to hysteroscopy alone and laparoscopy alone in the diagnosis of the ovulatory infertile female. It is short-time consumed, less traumatic, rapidly recovered. The postoperative pregnancy outcomes were satisfactory.Conclusions Laparoscopy combined with hysteroscopy is recommended as the primary precedure to the diagnosis and treatment of the ovulatory infertile female currently. It can cure some patients and provide reliable answer to whether assisted reproductive technique is needed as well.

Objective To investigate the effect of small dose of doxepin combined with psychological behav-ior intervention in the treatment of insomnia.Methods 21 patients with insomnia were selected as the study sub-jects,took doxepin 25mg before going to bed at every night,to the research object and psychological behavior interven-tion by hand,continuous treatment for 8 weeks.Used the method of questionnaire survey before and after two periods of time,assessed by using pittsburgh Sleep Quality mder(PSQI)scale,analysed the correlation between sleep quality and sleep beliefs attitudes.Results After treatment with 8 weeks,subjected sleep quality,sleep difficulties,sleep time,sleep efficiency,sleep disturbance,hypnotic drug use,and daytime functional scores were lower than before treat-ment (t =17.34,16.65,10.54,13.37,11.65,7.66,11.57,all P <0.05 ),the difference of the score of PSQI between after treatment[(5.80 ±0.97)points]and before treatment[(13.34 ±2.28)points]was significant(t =21.27,P <0.01).Conclusion Small dose of doxipin combined with psychological behavior intervention in treating insomnia can improve the sleep quality of patients.

Objective:To study the public health Emergency Operations Centers (EOCs)in the US, the European Union, the UK and Australia, and summarize the good practice for the improvement of National Health Emergency Response Command Center in Chinese National Health and Family Planning Commission.Methods:Literature review was conducted to explore the EOCs of selected countries.Results:The study focused on EOC function, organizational structure, human resources and information management. The selected EOCs had the basic EOC functions of coordinating and commanding as well as the public health related functions such as monitoring the situation, risk assessment, and epidemiological briefings. The organizational structures of the EOCs were standardized, scalable and flexible. Incident Command System was the widely applied organizational structure with a strong preference. The EOCs were managed by a unit of emergency management during routine time and surge staff were engaged upon emergencies. The selected EOCs had clear information management framework including information collection, assessment and dissemination.Conclusions:The performance of National Health Emergency Response Command Center can be improved by learning from the good practice of the selected EOCs, including setting clear functions, standardizing the organizational structure, enhancing the human resource capacity and strengthening information management.