No abstract available.
Vulva*

Cell growth rate and the secretion of ectopin hormones such as human chorionic gonadotropin(hCG), free beta-subunit and beta core fragment of hCG, were measured in the culture media with various serum concentrations from ovarian carcinoma cell lines, Bixler and Bix2NMB. The concentrations of fetal bovine serum contained in the culture media that showed the most active cell growth and hormone secretion were investigated to decide the best optimal serum concentration as one of the appropriate culture conditions in the experiment using cell culture. Ovarian concer cells were not able to proliferate in the media without serum. Cells proliferated more actively in higher concentration of serum. They secreted more hormone according to the increment of confluency as long as they secreted hormone. These findings suggest that serum is essential for surviving and cellular activity of cancer cells. The selection of the optimal serum concentration of culture media is very important to get appropriate data from the experiments which use cell culture technique. It is considered that we must have the knowledge on the most efficient concentration of serum in the culture media or on the exact percentage of serum in the media that matched to that of in vivo.
Cell Culture Techniques ; Cell Line ; Chorion ; Chorionic Gonadotropin* ; Culture Media* ; Humans* ; Ovarian Neoplasms*

No abstract available.
Liver Diseases* ; Liver* ; Pregnancy*

OBJECTIVE: Recently, telomerase activity was found in normal physiologically regenerating cells as well as cancer cells or germ cells. Human endometrium is also physiologically regenerating tissues and undergoes regular and dynamic changes during the menstrual cycle. So the authors examined normal human endometrial tissues for telomerase activity according to menstrual cycle. METHOD: In the current study, 66 normal human endometrial tissues were analyzed for telomerase activity by a radioisotope polimerase chain reaction-based telomeric repeat amplification protocol assay. RESULTS: Of 27 proliferative phase endometrial samples, 22(81%) expressed telomerase activity, whereas 10 of 23(43%) secretory phase sample, 2 of 10(10%) postmenopausal sample, 1 of 6(17%) pregnant endometrial sample did(P<0.05). 4 of 27(15%) endometrial samples in the proliferative phase expressed high telomerase activity after 100-fold dilution of extract, whereas 1 of 23(4%) from secretory phase, none of the 10 postmenopausal and 6 pregnant endometrial samples did. The highest activity was observed in late proliferative phase. CONCLUSION: This study indicates that normal endometrium expresses telomerase, the activity of which changes dramatically and regularly during the course of the menstrual cycle.
Endometrium* ; Female ; Germ Cells ; Humans ; Menstrual Cycle* ; Telomerase*

To evaluate the effects of pregnancy-induced hypertension (PIH) to the iron status of fetuses, umbilical cord blood of 35 newborn borne by PIH mothers and of 37 normal term infants delivered at Yeungnam University Hospital from September 1, 1993 to September 30, 1994, were studied. The serum hemoglobin concentration of women with PIH was significantly higher than normal full-term pregnant women. There was no significant difference in serum hemoglobin concetration between women with PIH and normal full-term pregnant women and their newborn infants. There was no significant difference in serum hemoglobin concentration between infants iof women with PIH and normal full-term infants. The serum iron concentration of newborn infants of women with PIH was higher and the serum ferritin concentration of newborn infants of women with PIH was lower than normal full-term infants, but there were no significant difference between the two groups. The serum total iron-binding capactity and unsaturated iron-binding capacity of infants of women with PIH were significantly higher than normal full-term infants. The newborn infants of PIH women seemed that they might have occult depletion of iron store and need meticulous follow up during early neonatal period.
Female ; Ferritins ; Fetal Blood ; Fetus ; Follow-Up Studies ; Humans ; Hypertension* ; Hypertension, Pregnancy-Induced ; Infant ; Infant, Newborn* ; Iron ; Mothers* ; Pregnancy ; Pregnant Women

No abstract available.
Humans ; Mothers*

Meigs' syndrome consists of an ovarian fibroma, or fibroma-like benign tumor, associated with fluid in both abdomen and chest. Characteristically, the ascites and hydrothorax disappeared and do not recurred following removal of the ovarian tumor. The authors presented a case of Meigs' syndrome that was cured by surgical removal of ovarian tumor and resulted in normal pregnancy outcome in 27-year-old pregnant woman with a brief review of literatures.
Abdomen ; Adult ; Ascites ; Female ; Fibroma ; Humans ; Hydrothorax ; Meigs Syndrome* ; Pregnancy Outcome ; Pregnancy* ; Pregnant Women ; Thorax

No abstract available.
Twins, Conjoined*

No abstract available.
Twins, Conjoined*

Vaginal leiomyoma is rare. They usually arise from the anterior vaginal wall and may be confused with a variety of vaginal tumors. Correct preoperative diagnosis is difficult. We experienced leiomyoma of suburethral area. We report this case with a brief review of literature.
Diagnosis ; Leiomyoma* ; Vagina*