No abstract available.
Vulva*

Cell growth rate and the secretion of ectopin hormones such as human chorionic gonadotropin(hCG), free beta-subunit and beta core fragment of hCG, were measured in the culture media with various serum concentrations from ovarian carcinoma cell lines, Bixler and Bix2NMB. The concentrations of fetal bovine serum contained in the culture media that showed the most active cell growth and hormone secretion were investigated to decide the best optimal serum concentration as one of the appropriate culture conditions in the experiment using cell culture. Ovarian concer cells were not able to proliferate in the media without serum. Cells proliferated more actively in higher concentration of serum. They secreted more hormone according to the increment of confluency as long as they secreted hormone. These findings suggest that serum is essential for surviving and cellular activity of cancer cells. The selection of the optimal serum concentration of culture media is very important to get appropriate data from the experiments which use cell culture technique. It is considered that we must have the knowledge on the most efficient concentration of serum in the culture media or on the exact percentage of serum in the media that matched to that of in vivo.
Cell Culture Techniques ; Cell Line ; Chorion ; Chorionic Gonadotropin* ; Culture Media* ; Humans* ; Ovarian Neoplasms*

No abstract available.
Liver Diseases* ; Liver* ; Pregnancy*

OBJECTIVE: Recently, telomerase activity was found in normal physiologically regenerating cells as well as cancer cells or germ cells. Human endometrium is also physiologically regenerating tissues and undergoes regular and dynamic changes during the menstrual cycle. So the authors examined normal human endometrial tissues for telomerase activity according to menstrual cycle. METHOD: In the current study, 66 normal human endometrial tissues were analyzed for telomerase activity by a radioisotope polimerase chain reaction-based telomeric repeat amplification protocol assay. RESULTS: Of 27 proliferative phase endometrial samples, 22(81%) expressed telomerase activity, whereas 10 of 23(43%) secretory phase sample, 2 of 10(10%) postmenopausal sample, 1 of 6(17%) pregnant endometrial sample did(P<0.05). 4 of 27(15%) endometrial samples in the proliferative phase expressed high telomerase activity after 100-fold dilution of extract, whereas 1 of 23(4%) from secretory phase, none of the 10 postmenopausal and 6 pregnant endometrial samples did. The highest activity was observed in late proliferative phase. CONCLUSION: This study indicates that normal endometrium expresses telomerase, the activity of which changes dramatically and regularly during the course of the menstrual cycle.
Endometrium* ; Female ; Germ Cells ; Humans ; Menstrual Cycle* ; Telomerase*

To evaluate the effects of pregnancy-induced hypertension (PIH) to the iron status of fetuses, umbilical cord blood of 35 newborn borne by PIH mothers and of 37 normal term infants delivered at Yeungnam University Hospital from September 1, 1993 to September 30, 1994, were studied. The serum hemoglobin concentration of women with PIH was significantly higher than normal full-term pregnant women. There was no significant difference in serum hemoglobin concetration between women with PIH and normal full-term pregnant women and their newborn infants. There was no significant difference in serum hemoglobin concentration between infants iof women with PIH and normal full-term infants. The serum iron concentration of newborn infants of women with PIH was higher and the serum ferritin concentration of newborn infants of women with PIH was lower than normal full-term infants, but there were no significant difference between the two groups. The serum total iron-binding capactity and unsaturated iron-binding capacity of infants of women with PIH were significantly higher than normal full-term infants. The newborn infants of PIH women seemed that they might have occult depletion of iron store and need meticulous follow up during early neonatal period.
Female ; Ferritins ; Fetal Blood ; Fetus ; Follow-Up Studies ; Humans ; Hypertension* ; Hypertension, Pregnancy-Induced ; Infant ; Infant, Newborn* ; Iron ; Mothers* ; Pregnancy ; Pregnant Women

Apgar score is most widely used evaluating indicator of newborn health, but it is very subjective. Umbilical cord blood gas analysis is more objective and effective than Apgar score in evaluation of newborn status. Cord blood gas was changed slightly by processing of time after fetal birth. This study was undertaken to observe objectiveness and effectiveness of umbilical arterial blood gas analysis and effects of time interval of cord clamping to newborn health with 122 pregnant women and their babies. We observed following results: 1. There were poor correlation between Apgar score and umbilical cord arterial blood analysis in evaluating of newborn health (P>0.05). 2. There was no clinical significance of Apgar score or umbilical arterial blood gas analysis as single indicator in evaluating of newborn health (P>0.05). 3. Gas analysis and pH of umbilical arterial blood was more helpful in evaluating of newborn health than Apgar score. 4. There were no significant effects of time interval of umbilical cord clamping to newborn health. 5. If there were no indications of early umbilical cord clamping, cord blood sampling at immediately after birth without cord clamping was more effective to evaluate newborn status.
Apgar Score* ; Blood Gas Analysis ; Constriction ; Cordocentesis ; Female ; Fetal Blood ; Humans ; Hydrogen-Ion Concentration* ; Infant Health* ; Infant, Newborn* ; Parturition ; Pregnant Women ; Umbilical Cord

Meigs' syndrome consists of an ovarian fibroma, or fibroma-like benign tumor, associated with fluid in both abdomen and chest. Characteristically, the ascites and hydrothorax disappeared and do not recurred following removal of the ovarian tumor. The authors presented a case of Meigs' syndrome that was cured by surgical removal of ovarian tumor and resulted in normal pregnancy outcome in 27-year-old pregnant woman with a brief review of literatures.
Abdomen ; Adult ; Ascites ; Female ; Fibroma ; Humans ; Hydrothorax ; Meigs Syndrome* ; Pregnancy Outcome ; Pregnancy* ; Pregnant Women ; Thorax

No abstract available.
Humans ; Mothers*

Vaginitis is one of the most common disease in gynecologic field in recent days. About 90% of these patients suffer from the infection of the vagina caused by Gardnerella vaginalis, Candida, or Trichomonas, either alone or in combination. For the effective diagnosis and management, it is essential to get an accurate identification of the causative agent. Applying simple and easy diagnostic methods such as pH of vaginal discharge, amine test and microscopic examination of wet mount preparation of normal saline and 10% KOH to 549 cases of randomly selected patients visiting Out-patient Department of Obstetrics and Gynecology of Yeungnam University Hospital through May 1st to Sept. 31st, 1987, the following results were obtained. 1. In the Gardnerella vaginitis vaginal pH was more than 5.0 in 81.0% of total cases, positive amine test in 62.8%, decreased lactobacilli in 77.4% & decreased WBC counts in 70.1%, In the Trichomonas vaginitis vaginal pH more than 5.0 was noted in 93.3% of the cases, negative amine test in 90%, decreased showed vaginal pH of 3.0~4.0 in 83.2%, negative amine test in 90%, decreased lactobacilli in 90% & increased WBC counts in 93.3%, whereas Candida vaginitis and normal groups showed vaginal pH of 3.0~4.0 in 83.2%, negative amine test in 100%, normal distribution of lactobacilli in 89.7%, normal WBC counts in 72.4%. 2. The accuracy rates of physical diagnosis by wet mount preparation of normal saline and 10% KOH revealed 26.3%, 47.5%, 70.0% in Gardnerella vaginitis, Candida vaginitis, and Trichomonas vaginitis, respectively. The vaginal pH and amine test showed 83.2% of accuracy rate in Candida vaginitis group, 60.6% in Gardnerella vaginitis group and 83.3% in Trichomonas vaginitis group. 3. In 23 cases of Gardnerella vaginitis showing vaginal pH of 3.0 or 4.0 and positive amine test, the clue cells were observed in 10% or less in 12 cases, 30% in 5 cases, 50% in 4 cases and 50% or more in 2 cases. In summary vaginal pH and amine test could be useful in screening and differentiating the different types of infectious vaginitis. Furthermore by adding microscopic examination of normal saline and 10% KOH wet mount preparation to them, they not only could obtain higher diagnostic accuracy rate but would be more valuable in selecting the patients requiring cultures to confirm the diagnoses.
Candida ; Diagnosis ; Gardnerella ; Gardnerella vaginalis ; Gynecology ; Humans ; Hydrogen-Ion Concentration* ; Mass Screening ; Obstetrics ; Outpatients ; Trichomonas ; Trichomonas Vaginitis ; Vagina ; Vaginal Discharge* ; Vaginitis*

The World Association for Sexology (WAS) will adopt a Declaration of Sexual Right as basic and fundamental human lights August this year in Hong Kong. Two years ago WAS has been developing a declaration of Sexual Rights in order to further promote sexual health and protect the sexual rights of everyone. Undoubtedly sexuality is one of the most intriguing subject in the area of human behavior abd psychology, and has been so since antiquity. The influence of sexual factors of human on all aspects of the society has been immense. The varieties of marriage, from traditional to the open marriage model are presented in front of our sight. The influences of women's movement in Korea, which has produced sexual, psychological, economic, political, familial and social changes look so small and the changes are not that much in America. But the profound effects of the woman's movement affect on Korean society being realized as man's view of woman and woman's view of herself undergo constant change and revaluation. With increased sexual awareness on the part of the public owing to mass media, for example AH-U-SEONG aired in TV and with increased emphasis on sexual matters in daily life, more and more physicians are being asked to deal with and manage sex-related problems in thei daily practice. Yet, despite the obvious need for informed sexual counseling, doctors are often uncomfortable about sexual matters and many physicians have no special expertise in this area. So physicians concerned in sexology especially such as gynecologists, urologists, psychiatrists and endocrinologists must realize well about sex-related knowledge which is needed for counseling the patient as medical consumer and gap between reality and ideality in sexual diagnosis and treatment. Establishment of management system for the people who have sexual problems is strongly needed to promote sexual health and protect the sexual rights of everyone and for the Sexual Rights as basic and fundamental human rights.
Americas ; Counseling ; Diagnosis ; Female* ; Hong Kong ; Human Rights ; Humans ; Korea* ; Marriage ; Mass Media ; Psychiatry ; Psychology ; Reproductive Health ; Sexology* ; Sexuality ; Social Change